17 results about "Complex carbohydrate" patented technology

The present invention relates to a method for hydrolyzing carbohydrates present in raw materials containing carbohydrates, comprising the step a) of contacting the raw material containing carbohydrate with a medium in which bacteria capable of secreting carbohydrate-hydrolyzing enzymes have been fermented, to a medium in which bacteria capable of secreting carbohydrate-hydrolyzing enzymes have been fermented, to a method for simultaneously liquifying and saccharifying starch, a method for simultaneously liquifying, saccharifying and fermenting starch, and to a method for producing a biomass, the method comprising the step of cultivating bacteria secreting carbohydrate-hydrolyzing enzymes on a medium comprising complex carbohydrates as a major carbon source.

The invention belongs to the field of microbial fermentation, and particularly relates to a plant lactobacillus fermentation extract for improving sleep. According to the method, through the synergistic effect of double enzymes, the degradation efficiency of complex carbohydrates in the raw materials is greatly improved, a substrate capable of being utilized by zymophyte is released to the maximum extent, and the generation amount of GABA is remarkably increased. In addition, fermentation strains are reasonably optimized, and when a small amount of clostridium butyricum assists lactobacillus plantarum in fruit and vegetable fermentation, the clostridium butyricum can play a synergistic role and promote generation of GABA (gamma-aminobutyric acid). Xylose is added as an additional carbon source, so that the problem of carbon source supplementation is avoided, and the fermentation environment is optimized. Compared with the traditional method, the fermentation extract prepared by the method disclosed by the invention is more excellent in sleep aiding effect, higher in GABA content and obvious in sleep improving function, and has a wide application prospect. The method has obvious advantages in the aspects of technical innovation and functional effects.

A composition for effervescing and flavoring water includes 20 to 45 weight percent flavor enhancing ingredient; 10 to 35 weight percent sodium bicarbonate; 10 to 20 weight percent citric acid; 10 to 45 weight percent complex carbohydrate; one or more of: (i) a first natural sweetener comprising 0.1 to 2 weight percent Rebaudioside M; and (ii) a second natural sweetener comprising 0.1 to 2 weight percent Rebaudioside A. The composition is configured to generate bubbles in the water when added to the water and to add flavor and sweetness to the water.

The present invention provides nutritional supplements consisting of an alginate hydrogel with sub-saturating amounts of calcium ions and comprising an aqueous solution comprising active ingredients, selected from sugars, complex carbohydrates, electrolytes, caffeine, and amino acids. The nutritional supplements allow for intake of carbohydrates without causing oral pH lowering and risk of dental caries, and without causing gastrointestinal discomfort while maintaining a high rate of intestinal uptake and oxidation of carbohydrates.

Glycan-binding proteins, and compositions thereof, are generally described, including methods of making and using such proteins. The proteins may include scaffolds based on easily evolvable DNA-binding proteins, with binding sites able to specifically bind to mono- or disaccharides, such as monosaccharide-binding determinants, disaccharide-binding determinants, more complex carbohydrates, etc. In certain aspects, a protein may be generated starting from a small DNA-binding protein, such as Sso7d. Such glycan-binding proteins may have numerous applications, including in enzyme-linked immunosorbent assays (ELISAs), glycan characterization, cell selection, flow cytometry, histology, imaging, arrays, affinity purification, enzyme-linked visualization, binding to a target for pharmaceutical purposes, etc.

Disclosed is a composition comprising 20-80% by weight of total fatty matter; 0.1 to 5 wt% of an electrolyte; 0.001% to 5% by weight of a PPAR activator selected from the group consisting of 10-hydroxystearic acid or 12-hydroxystearic acid; 1-45% by weight of a composite carbohydrate comprising starch; and 10 to 50% by weight of water.

A novel analytical system and method to analyze complex carbohydrates such as human milk oligosaccharides by low-temperature High-Performance Anion Exchange Chromatography with Pulsed Amperometric Detection and High-resolution Mass Spectrometry (HPAE-PAD-MS). The analytical system controls the temperature of the column, electrochemical detector, and ion removal device at or below 15° C. The HPAE-PAD workflow with high-resolution mass spectrometry provides useful molecular structure information. It facilitates the detection of milk oligosaccharides, particularly unknown structures, without the use of analytical standards.

The invention discloses a multi-vitamin taurine electrolyte solid beverage capable of efficiently supplementing water in a balanced manner and improving physical ability, and relates to the technical field of special diet food. The solid beverage consists of a compound carbohydrate matrix, an electrolyte system, a compound nutrition enhancer, taurine and a food additive, the composite carbohydrate matrix takes isomaltooligosacharide, inulin and erythritol as cores, the electrolyte system comprises potassium citrate, edible salt and sodium citrate, and the compound nutrition enhancer contains B vitamins and vitamin C; according to the beverage, exogenous cane sugar, protein and fat are not added; all the components have a synergistic effect, essential nutrients are provided while water and electrolyte are supplemented, and the energy is low. The sports beverage solves the problems that an existing sports beverage is too high in energy, single in nutrition and unbalanced in water and energy supplementation, is suitable for people with a large amount of sweating, and achieves the dual effects of efficient balanced water supplementation and physical ability improvement.

This invention provides a complete nutritional food that can be used as a meal replacement, its preparation method, and its application. The food is scientifically formulated from natural ingredients and medicinal and edible homologous components, including glutinous rice flour, kudzu root powder, yam powder, wolfberry extract, jujube extract, camel milk powder, dehydrated egg flower powder, soybean flour, sea cucumber powder, ginseng powder, turtle shell powder, dried ginger extract, codonopsis extract, astragalus extract, eucommia extract, sesame oil, and salt. This complete nutritional food covers the basic nutrients required by the human body, including high-quality protein, complex carbohydrates, healthy fats, essential minerals, vitamins, dietary fiber, sodium, and various beneficial components with physiological activity, such as total polysaccharides and total flavonoids. Its nutritional structure is balanced and scientifically proportioned. Long-term consumption as a meal replacement or substitute can not only meet daily energy and nutritional needs but also avoid nutrient deficiencies or metabolic imbalances, exhibiting good safety and sustainability. Therefore, it can be used as a complete nutritional meal replacement for daily dietary substitution.

A composition of several nutrient powders containing a mixture (10) of physical powders, wherein the mixture (10) of physical powders comprises several stable building blocks (11), wherein the respective stable building block (11) contains: a first powder (12) of functional ingredients having a content of 27.5~32.5% and being selected from a group of ingredients consisting of physically or chemically converted dry, stable substances, wherein the first powder (12) of functional ingredients has properties of organic acid salts with a molecular mass of 1,000 to 10,000 Da, properties of the structure of soluble short-chain carbohydrates, properties of the polymer structure of acidic polysaccharides, and properties of fragments of amino group-containing complex carbohydrates, wherein the first powder (12) of functional ingredients is non-bioactive, can be stably produced and homogenized in a production process for food-safe agents, and is heat-resistant; a carbohydrate powder (13) made of oligosaccharides, which has a content of 24.5~29.5% and is tasteless and readily soluble; a food-grade structurally stable powder (14) having a content of 12.5∼17.5%, produced from natural ingredients by drying and refining, containing polysaccharides and protein segments, having natural flavors, and containing neither biological ingredients nor cellular tissue; a second powder (15) of functional ingredients having a content of 10-15%, a curly or irregular particle structure, capable of being physically converted into a dry powder from plant or naturally derived substances, and having stabilizing and nutritional supplementary effects; an acerola fruit powder (16) which has a content of 5.5∼10.5% and contains natural vitamin C and antioxidants; and an elderberry powder (17) having a content of 5-10.0%, containing anthocyanidins and providing the desired taste and color of the composition according to the invention.

The invention provides an enzyme-labeled antibody protective agent and application of the enzyme-labeled antibody protective agent in an ELISA (Enzyme-Linked Immunosorbent Assay) detection kit. The provided enzyme-labeled antibody protective agent is prepared from 0.1 to 0.5 percent of aminated mesoporous silicon dioxide nano particles, 0.5 to 2 percent of bovine serum albumin, 5 to 9 percent of complex saccharides, 0.01 to 0.1 percent of poloxamer 188, 0.01 to 0.05 percent of reduced glutathione and 0.02 to 0.05 percent of Proclin-300. The protective agent provided by the invention can effectively maintain the structure and activity of the enzyme-labeled antibody under various adverse conditions such as temperature fluctuation, oxidative stress and repeated freezing and thawing. The protective agent has broad-spectrum stability, not only can significantly reduce the activity loss of the antibody in the storage, transportation and use processes, but also can maintain the native conformation of the enzyme and the antibody by slowing down the oxidation effect, thereby avoiding inactivation and aggregation, and significantly prolonging the storage life and the use cycle of the antibody.

There is a need for analytical methods to identify and quantify complex carbohydrate compositions, particularly methods using liquid chromatography-mass spectrometry ("LC-MS systems") to analyze the polysaccharide components of glycoproteins in samples. For example, there is a demand for the use of LC-MS systems in process control during the production of complex carbohydrates. [Solution] A method for analyzing the polysaccharide components of a glycoprotein in a sample, comprising: identification of the polysaccharide components; and absolute quantification of the polysaccharide components, the method comprising: (a) establishing a calibration curve for the polysaccharide components using an LC-MS system; (b) measuring the sample using the same LC-MS system; and (c) comparing the results of (a) and (b) thereby analyzing and comparing the polysaccharide components in the sample, wherein the glycoprotein is in its natural state.

The invention relates to the technical field of oral liquid, in particular to angelica sinensis blood-enriching oral liquid and a preparation process thereof. Comprising the following steps: preparing an angelica sinensis water extract, preparing an angelica sinensis fermentation liquor and preparing the angelica sinensis blood-enriching oral liquid. The lactic acid bacteria and the bifidobacteria are cultured, the symbiotic relationship is established according to a specific proportion for fermentation, the number of the lactic acid bacteria is dominant, acid can be rapidly produced, the pH value of the environment is reduced, stricter anaerobic and acid environments are created, the growth of the strictly anaerobic bifidobacteria is facilitated, the lactic acid bacteria can rapidly start acidification, and the fermentation efficiency is improved. In addition, the lactic acid bacteria can quickly utilize simple carbohydrates, the bifidobacteria utilize complex carbohydrates, the lactic acid bacteria and the bifidobacteria cooperate to metabolize, the substrate utilization rate is increased, organic acids generated by the lactic acid bacteria and the bifidobacteria jointly create a bacteriostatic environment, and the antibacterial activity of the bifidobacteria is improved. And the content of nutrient substances in the Chinese angelica fermentation liquor is increased by more comprehensively converting the substrate.

The invention discloses application of a cordyceps sinensis mycelium fermentation culture in promotion of calf growth, and belongs to the technical field of animal breeding. The cordyceps sinensis mycelium fermentation culture is obtained by performing fermentation culture on cordyceps sinensis liquid, performing solid-liquid separation, collecting a liquid part, and performing concentration and spray drying. Research finds that the cordyceps sinensis mycelium fermentation culture can effectively enhance the body resistance of calves, reduce diarrhea and cold morbidity, remodel rumen micro-ecology, improve microbial population structure, and up-regulate complex carbohydrate decomposition and galactose metabolic pathway in rumen microorganisms, so as to improve the immunity of calves. The digestion and utilization potential of the calves on nutrient substances such as fibers is remarkably improved, and protein and energy metabolism is optimized, so that the growth of the calves is promoted.

The invention discloses nutrition-balanced Chinese yam, radix puerariae and corn soup and a preparation method thereof. The nutrition-balanced Chinese yam, radix puerariae and corn soup is prepared from, by weight, 300-400 g of corn flour, 50-80 g of Chinese yam powder, 30-60 g of radix puerariae powder, 10-20 g of lotus seed powder, 10-20 g of poria cocos powder, 5-15 g of fructus lycii, 5-15 g of white hyacinth beans, 5-15 g of coix seeds and 5-15 g of hericium erinaceus. Balanced proportioning of carbohydrates, proteins, dietary fibers, vitamins and minerals is realized, the formula is rich in various active ingredients, such as Chinese yam polysaccharide, puerarin and hericium erinaceus polysaccharide, and the meal replacement powder is helpful for tonifying spleen, nourishing stomach and enhancing immunity after being eaten for a long time, realizes unification of nutritional supplement and function conditioning, is far superior to a common meal replacement product with a single ingredient, and is suitable for popularization and application. According to the present invention, the corn, the Chinese yam and the radix puerariae in the formula are rich in composite carbohydrates and dietary fibers so as to provide lasting satiety, and the whole fat content is low so as to easily perform scientific body weight management and calorie control, such that the natural flavor and the nutrition of the food material are retained, and the requirement of the high-end health food on the clean label is met; the corn, the Chinese yam and the radix puerariae in the formula are rich in the composite carbohydrates and the dietary fibers, and can provide the lasting satiety;

This invention relates to functional food technology, specifically the formulation and manufacturing process of corn-based snack bars for athletes. The main ingredient used is corn flour, combined with soybeans, roasted almonds, honey, and mineral salts to produce a solid bar-shaped product containing complex carbohydrates, vegetable protein, and balanced healthy fats. The manufacturing process includes mixing dry and wet ingredients, molding, and low-temperature baking at 110–120°C to maintain nutritional value and natural flavor. The resulting product is crispy, highly nutritious, easy to consume, and has a long shelf life, as an alternative local functional food for athletes' energy recovery.

The invention discloses a glucoside hydrolase capable of degrading cellobiose and maltotriose. The glucoside hydrolase is characterized in that the amino acid sequence of the glucoside hydrolase is SEQ ID NO. 1. The strain has relatively high activity on cellobiose and maltotriose, the enzyme activities are respectively 0.1 U and 0.102 U, and the specific activities are respectively 80.4 U / mg and 82.0 U / mg. The invention aims to enrich the existing carbohydrate enzyme gene bank and develop a novel efficient enzyme preparation. According to the present invention, the novel efficient carbohydrate enzyme is explored, and has efficient degradation ability on cellobiose and maltotriose, such that the reference is provided for the exploration of the novel enteric microorganism encoded CAZYmes. CAZymes generated by animal intestinal microorganisms are beneficial to decomposition of complex carbohydrates, the carbohydrates are difficult to decompose in a digestive system of a host, and the carbohydrate active enzyme obtained through excavation is expected to improve the digestion efficiency. By studying CAZymes in intestinal microorganisms, a novel enzyme preparation is expected to be developed and used in food industry, feed industry or as a therapeutic agent.