Angelica sinensis blood-enriching oral liquid and preparation process thereof
By using symbiotic fermentation with lactic acid bacteria and bifidobacteria and adding specific nutrients, the problem of low release rates of angelica polysaccharides and ferulic acid was solved, improving the nutritional value and stability of the oral liquid and enhancing its blood-tonifying effect.
Patent Information
- Application Number
- CN202511596910.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-11-04
- Publication Date
- 2025-12-09
AI Technical Summary
In the existing technology, the symbiotic effect of lactic acid bacteria and bifidobacteria is not good during the fermentation process of Angelica polysaccharide, resulting in low polysaccharide release rate and unstable ferulic acid content, making it difficult to effectively improve the blood-tonifying effect.
By cultivating lactic acid bacteria and bifidobacteria to establish a symbiotic relationship, adding nutrients such as cysteine and manganese sulfate, a compound bacterial agent is formed. This agent is then fermented with donkey-hide gelatin, red algae polysaccharides, and ferric sulfate. Angelica sinensis water extract is then added, followed by the addition of sodium alginate and β-cyclodextrin to form a stable oral liquid.
It improved the release rate and stability of polysaccharides and ferulic acid, enhanced the nutritional value and bioavailability of the oral liquid, and improved its taste and stability.
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Figure CN121081518A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of oral liquid technology, specifically to an Angelica sinensis blood-tonifying oral liquid and its preparation process. Background Technology
[0002] The Chinese herbal medicine Angelica sinensis refers to the dried root of Angelica sinensis, a plant in the Apiaceae family. It is rich in Angelica sinensis polysaccharides, ferulic acid, vitamin B12, folic acid, etc. It can stimulate bone marrow hematopoiesis, increase the level of red blood cells and hemoglobin, and improve blood deficiency, chlorosis, dizziness and palpitations. In traditional Chinese medicine, it has the functions of nourishing blood, moistening the intestines and relieving constipation.
[0003] Conventional methods for preparing Angelica sinensis extract include alcohol extraction and water extraction. After industrial improvements, the dissolution efficiency of these two methods for Angelica sinensis polysaccharides is similar. Furthermore, water extraction is simpler, lower in cost, and can retain water-soluble polysaccharides, ferulic acid, amino acids, choline, and other nutrients, exhibiting clear anti-inflammatory and hematopoietic effects. However, the polysaccharides extracted by water extraction are macromolecular polysaccharides, with smaller polysaccharides like Angelica sinensis polysaccharides aggregated within them. This results in lower absorption efficiency of macromolecular polysaccharides by the human body. Therefore, fermentation of the Angelica sinensis water extract is necessary to convert the macromolecular polysaccharides and other lower-molecular polysaccharides. To improve the activity of Angelica sinensis extract, existing fermentation techniques for Angelica sinensis extract, while capable of rapidly acidifying the environment, lack the ability to decompose cellulose and pectin, resulting in low release rates of Angelica sinensis polysaccharides. Strictly anaerobic bacteria such as Bifidobacteria struggle to proliferate due to oxygen stress and insufficient substrate in the early stages of fermentation, limiting their metabolic activity. Furthermore, the lack of nutritional regulation methods for the symbiotic relationship between these two bacteria leads to unstable content of active ingredients such as ferulic acid in the fermentation products. Therefore, this invention provides an Angelica sinensis blood-tonifying oral liquid and its preparation process to address the aforementioned problems in the prior art. Summary of the Invention
[0004] In view of the shortcomings of the existing technology, the purpose of this invention is to provide an Angelica sinensis blood-tonifying oral liquid and its preparation process.
[0005] A preparation process for an Angelica sinensis blood-tonifying oral liquid includes the following steps:
[0006] S1: Preparation of Angelica sinensis aqueous extract
[0007] Angelica sinensis was frozen, pulverized, and sieved. Then it was added to pure water and decocted twice. The filtrates from the two decoctions were combined to obtain Angelica sinensis aqueous extract.
[0008] S2: Preparation of Angelica sinensis fermentation broth
[0009] Lactic acid bacteria were inoculated into the culture medium, and then cysteine and manganese sulfate were added to obtain a lactic acid bacteria culture. Bifidobacterium was inoculated into the culture medium, and then Angelica sinensis water extract was added to obtain a Bifidobacterium culture. The lactic acid bacteria culture and Bifidobacterium culture were mixed to obtain a compound bacterial agent. Donkey-hide gelatin, red algae polysaccharide and ferric sulfate were added to the Angelica sinensis water extract to obtain a water extract base liquid. The compound bacterial agent was added to the water extract base liquid and cultured to obtain the Angelica sinensis extract fermentation broth.
[0010] S3: Preparation of Angelica sinensis blood-tonifying oral liquid
[0011] Angelica extract fermentation broth was added to an ethanol-water solution for precipitation to remove protein and polysaccharide impurities. The supernatant was concentrated to obtain Angelica fermentation concentrate. The Angelica fermentation concentrate was sterilized, and sodium alginate was added. The mixture was stirred and cooled, and β-cyclodextrin was added. The mixture was stirred, and xylitol and glycine ferrous chelate were added to obtain oral liquid stock solution. The solution was degassed to eliminate foam and dissolved oxygen, and then filled with nitrogen to obtain Angelica blood-tonifying oral liquid.
[0012] Furthermore, the preparation of Angelica sinensis aqueous extract by S1 includes the following steps:
[0013] Angelica sinensis was frozen at -20 to -15°C for 3-4 hours, then pulverized and passed through a 300-mesh sieve. It was then added to purified water at 10 to 15°C with a material-to-liquid ratio of 1g:(8-10)mL and soaked for 8-12 hours. The mixture was then heated to 95 to 100°C and decocted until it was reduced to one-fifth of its original volume. The mixture was filtered to obtain a primary filtrate. The residue was mixed with purified water at a material-to-liquid ratio of 1g:(6-8)mL and decocted again at 95 to 100°C until it was reduced to one-fifth of its original volume. The mixture was then filtered to obtain a secondary filtrate. The primary and secondary filtrates were mixed and stirred for 2-3 minutes to obtain an aqueous extract of Angelica sinensis.
[0014] Furthermore, the preparation of Angelica sinensis fermentation broth by S2 includes the following steps:
[0015] S2.1: Inoculate lactic acid bacteria into the culture medium, then add 0.1-0.2% cysteine and 0.05-0.08% manganese sulfate by weight of the system. Shake and culture at 37-38℃ for 24-25 hours at a speed of 200-250 r / min to obtain lactic acid bacteria culture.
[0016] S2.2: Inoculate Bifidobacterium into the culture medium, then add 5-6% of Angelica sinensis water extract by weight of the system, adjust the pH to 5.5-6, and culture at 37-38℃ with shaking at 200-250 r / min for 24-25 h to obtain Bifidobacterium bacterial culture;
[0017] S2.3: Mix lactic acid bacteria and bifidobacteria in a mass ratio of 5:(2-3) to obtain a compound bacterial agent. Add donkey-hide gelatin, red algae polysaccharide and ferric sulfate to the Angelica sinensis water extract to obtain a water extract base liquid. Add the compound bacterial agent to the water extract base liquid for cultivation. The amount of compound bacterial agent added is 5-8% of the system mass. Then, shake and culture at 37-38℃ for 60-62h. Centrifuge the product at 4℃ at a speed of 8000-8100r / min for 10-15min to remove the precipitate and obtain the Angelica sinensis extract fermentation broth.
[0018] Furthermore, the preparation of Angelica sinensis blood-tonifying oral liquid by S3 includes the following steps:
[0019] S3.1: Add the fermentation broth of Angelica sinensis extract to 2-4 times the amount of ethanol aqueous solution and precipitate for 45-48 hours to remove protein and polysaccharide impurities. Concentrate the supernatant to one-fifth of the original volume at 45-50℃ and 0.1-0.2MPa to obtain Angelica sinensis fermentation concentrate.
[0020] S3.2: Sterilize the Angelica sinensis fermentation concentrate at 100-105℃ for 3-5 min, then add 0.1-0.3% sodium alginate by weight at 60-65℃, stir at 200-250 r / min for 20-25 min, then let it stand and cool to 45-50℃, add 1.5-2.5% β-cyclodextrin by weight, then start stirring, add 3-5% xylitol and 0.02-0.05% glycine ferrous chelate at 50-60 r / min to obtain the oral liquid stock solution, degas at -0.08 MPa and 40-45℃ for 15-20 min to eliminate foam and dissolved oxygen, then fill with nitrogen and bottle to obtain Angelica sinensis blood-tonifying oral liquid.
[0021] Furthermore, the culture medium is prepared by mixing 10-12 g / L peptone, 3-4 g / L beef extract, 5-6 g / L glucose and 5-7 g / L sodium chloride, and adjusting the pH of the system to 7-7.5 to obtain the culture medium.
[0022] Furthermore, the amount of donkey-hide gelatin, red algae polysaccharide, and ferric sulfate added is 5-6g of donkey-hide gelatin, 3-4g of red date polysaccharide, and 0.1-0.2g of ferric sulfate per 100mL of Angelica sinensis aqueous extract.
[0023] Furthermore, the concentration of the ethanol-water solution is 85-95%.
[0024] Furthermore, the glycine ferrous chelate is specifically Fe(C2H4NO2)2.
[0025] An oral liquid for replenishing blood with Angelica sinensis is prepared using the above-mentioned preparation process for oral liquid for replenishing blood with Angelica sinensis.
[0026] The present invention has the following advantages:
[0027] 1. This invention involves fermenting lactic acid bacteria and bifidobacteria in a specific ratio to establish a symbiotic relationship. The dominance of lactic acid bacteria enables rapid acid production, lowering the environmental pH and creating a more strictly anaerobic and acidic environment, which is conducive to the growth of strictly anaerobic bifidobacteria. This allows lactic acid bacteria to quickly initiate acidification while providing sufficient reaction start points and a suitable environment for bifidobacteria. Furthermore, lactic acid bacteria can quickly utilize simple sugars, while bifidobacteria utilize complex carbohydrates. The two work together to improve substrate utilization. The organic acids produced by both create an antibacterial environment, resulting in more comprehensive substrate transformation. By adding cysteine, manganese sulfate, donkey-hide gelatin, red algae polysaccharides, and ferric sulfate, sufficient and optimized nutritional and environmental conditions are provided for the growth and metabolism of microorganisms at different stages, thereby increasing the nutrient content in the Angelica sinensis fermentation broth.
[0028] 2. This invention adds sodium alginate to the Angelica sinensis fermentation broth to thicken and stabilize it, forming a weak gel network to prevent precipitation and stratification, improve the texture of the oral liquid, and combine with the active ingredients in the Angelica sinensis fermentation broth to encapsulate easily oxidized components, reduce degradation, delay release, and improve bioavailability. Furthermore, the hydrophobic cavity of β-cyclodextrin is used to encapsulate the gel network through molecular interactions, accommodating hydrophobic substances. Simultaneously, xylitol is added to increase the solution viscosity. Xylitol can synergistically form a uniform colloid with sodium alginate, preventing the precipitation of ferrous glycine. The reducing colloid formed by the synergistic effect of xylitol and sodium alginate protects ferrous iron from oxidation, avoids chelate dissociation, and improves the nutritional value of the oral liquid. Attached Figure Description
[0029] Figure 1 This is a flowchart illustrating the preparation process of the Angelica sinensis blood-tonifying oral liquid of the present invention. Detailed Implementation
[0030] To enable those skilled in the art to better understand the technical solutions of this invention, the technical solutions of this invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of this invention.
[0031] Example 1:
[0032] A preparation process for an Angelica sinensis blood-tonifying oral liquid, such as... Figure 1 As shown, it includes the following steps:
[0033] S1: Preparation of Angelica sinensis aqueous extract
[0034] Angelica sinensis was frozen at -20°C for 3 hours, then pulverized and passed through a 300-mesh sieve. It was then added to purified water at 10°C with a material-to-liquid ratio of 1g:8mL and soaked for 8 hours. The mixture was then heated to 95°C and decocted until it was reduced to one-fifth of its original volume. The filtrate was filtered to obtain the first filtrate. The residue was mixed with purified water at a material-to-liquid ratio of 1g:6mL and decocted again at 95°C until it was reduced to one-fifth of its original volume. The filtrate was filtered to obtain the second filtrate. The first and second filtrates were mixed and stirred for 2 minutes to obtain the Angelica sinensis aqueous extract.
[0035] S2: Preparation of Angelica sinensis fermentation broth
[0036] S2.1: Mix 10 g / L peptone, 3 g / L beef extract, 5 g / L glucose and 5 g / L sodium chloride and adjust the pH of the system to 7 to obtain a culture medium. Inoculate lactic acid bacteria into the culture medium, and then add 0.1% cysteine and 0.05% manganese sulfate by weight of the system. Shake and culture at 37°C for 24 h at 200 r / min to obtain a lactic acid bacteria culture.
[0037] S2.2: Inoculate Bifidobacterium into the culture medium, then add 5% of Angelica sinensis water extract by mass, adjust the pH to 5.5, and culture at 37℃ with shaking at 200 r / min for 24 h to obtain Bifidobacterium bacterial suspension;
[0038] S2.3: Mix lactic acid bacteria and bifidobacteria in a mass ratio of 5:2 to obtain a compound bacterial agent. Add donkey-hide gelatin, red algae polysaccharide and ferric sulfate to the Angelica sinensis aqueous extract. The amount of donkey-hide gelatin, red algae polysaccharide and ferric sulfate added is 5g donkey-hide gelatin, 3g jujube polysaccharide and 0.1g ferric sulfate per 100mL of Angelica sinensis aqueous extract to obtain the aqueous extract base liquid. Add the compound bacterial agent to the aqueous extract base liquid for culture. The amount of compound bacterial agent added is 5% of the system mass. Then, culture at 37℃ with shaking for 60h. Centrifuge the product at 8000r / min for 10min at 4℃ to remove the precipitate and obtain the Angelica sinensis extract fermentation broth.
[0039] S3: Preparation of Angelica sinensis blood-tonifying oral liquid
[0040] S3.1: Add the fermentation broth of Angelica sinensis extract to twice the concentration of 85% ethanol aqueous solution and precipitate for 45 hours to remove protein and polysaccharide impurities. The supernatant is concentrated to one-fifth of the original volume at 45℃ and 0.1MPa to obtain Angelica sinensis fermentation concentrate.
[0041] S3.2: Sterilize the Angelica sinensis fermentation concentrate at 100℃ for 3 min, then add 0.1% sodium alginate by weight at 60℃, stir at 200 r / min for 20 min, then let it stand and cool to 45℃, add 1.5% β-cyclodextrin by weight, then start stirring, add 3% xylitol and 0.02% glycine ferrous chelate Fe(C2H4NO2)2 at 50 r / min to obtain the oral liquid stock solution, degas at -0.08 MPa and 40℃ for 15 min to eliminate foam and dissolved oxygen, then fill with nitrogen and bottle to obtain Angelica sinensis blood-tonifying oral liquid.
[0042] Example 2:
[0043] A preparation process for an Angelica sinensis blood-tonifying oral liquid, such as... Figure 1 As shown, it includes the following steps:
[0044] S1: Preparation of Angelica sinensis aqueous extract
[0045] Angelica sinensis was frozen at -20°C for 3 hours, then pulverized and passed through a 300-mesh sieve. It was then added to purified water at 10°C with a material-to-liquid ratio of 1g:10mL and soaked for 8 hours. The mixture was then heated to 95°C and decocted until it was reduced to one-fifth of its original volume. The filtrate was filtered to obtain the first filtrate. The residue was mixed with purified water at a material-to-liquid ratio of 1g:8mL and decocted again at 95°C until it was reduced to one-fifth of its original volume. The filtrate was filtered to obtain the second filtrate. The first and second filtrates were mixed and stirred for 2 minutes to obtain the Angelica sinensis aqueous extract.
[0046] S2: Preparation of Angelica sinensis fermentation broth
[0047] S2.1: Mix 12 g / L peptone, 4 g / L beef extract, 6 g / L glucose and 7 g / L sodium chloride and adjust the pH of the system to 7 to obtain a culture medium. Inoculate lactic acid bacteria into the culture medium, and then add 0.2% cysteine and 0.08% manganese sulfate by weight of the system. Shake and culture at 37°C for 24 h at 200 r / min to obtain a lactic acid bacteria culture.
[0048] S2.2: Inoculate Bifidobacterium into the culture medium, then add 6% of Angelica sinensis water extract by mass, adjust the pH to 6, and culture at 37℃ with shaking at 200 r / min for 24 h to obtain Bifidobacterium bacterial culture;
[0049] S2.3: Mix lactic acid bacteria and bifidobacteria in a mass ratio of 5:3 to obtain a compound bacterial agent. Add donkey-hide gelatin, red algae polysaccharide and ferric sulfate to the Angelica sinensis aqueous extract. The amount of donkey-hide gelatin, red algae polysaccharide and ferric sulfate added is 6g donkey-hide gelatin, 4g jujube polysaccharide and 0.2g ferric sulfate per 100mL of Angelica sinensis aqueous extract to obtain the aqueous extract base liquid. Add the compound bacterial agent to the aqueous extract base liquid and culture. The amount of compound bacterial agent added is 8% of the system mass. Then, culture at 37℃ with shaking for 60h. Centrifuge the product at 8000r / min for 10min at 4℃ to remove the precipitate and obtain the Angelica sinensis extract fermentation broth.
[0050] S3: Preparation of Angelica sinensis blood-tonifying oral liquid
[0051] S3.1: Add the fermentation broth of Angelica sinensis extract to 4 times the concentration of 95% ethanol aqueous solution and precipitate for 45 hours to remove protein and polysaccharide impurities. The supernatant is concentrated to one-fifth of the original volume at 45℃ and 0.1MPa to obtain Angelica sinensis fermentation concentrate.
[0052] S3.2: Sterilize the Angelica sinensis fermentation concentrate at 100℃ for 3 min, then add 0.3% sodium alginate by weight at 60℃, stir at 200 r / min for 20 min, then let it stand and cool to 45℃, add 2.5% β-cyclodextrin by weight, then start stirring, add 5% xylitol and 0.05% glycine ferrous chelate Fe(C2H4NO2)2 at 50 r / min to obtain the oral liquid stock solution, degas at -0.08 MPa and 40℃ for 15 min to eliminate foam and dissolved oxygen, then fill with nitrogen and bottle to obtain Angelica sinensis blood-tonifying oral liquid.
[0053] Example 3:
[0054] A preparation process for an Angelica sinensis blood-tonifying oral liquid, such as... Figure 1 As shown, it includes the following steps:
[0055] S1: Preparation of Angelica sinensis aqueous extract
[0056] Angelica sinensis was frozen at -15°C for 4 hours, then pulverized and passed through a 300-mesh sieve. It was then added to purified water at 15°C at a material-to-liquid ratio of 1g:8mL and soaked for 12 hours. The mixture was then heated to 100°C and decocted until it reached one-fifth of its original volume. The filtrate was filtered to obtain the first filtrate. The residue was mixed with purified water at a material-to-liquid ratio of 1g:6mL and decocted again at 100°C until it reached one-fifth of its original volume. The filtrate was filtered to obtain the second filtrate. The first and second filtrates were mixed and stirred for 3 minutes to obtain the Angelica sinensis aqueous extract.
[0057] S2: Preparation of Angelica sinensis fermentation broth
[0058] S2.1: Mix 10 g / L peptone, 3 g / L beef extract, 5 g / L glucose and 5 g / L sodium chloride and adjust the pH of the system to 7.5 to obtain a culture medium. Inoculate lactic acid bacteria into the culture medium, and then add 0.1% cysteine and 0.05% manganese sulfate by weight of the system. Shake and culture at 38°C for 25 h at 250 r / min to obtain a lactic acid bacteria culture.
[0059] S2.2: Inoculate Bifidobacterium into the culture medium, then add 5% of Angelica sinensis water extract by mass, adjust the pH to 6, and culture at 38℃ with shaking at 250 r / min for 25 h to obtain Bifidobacterium bacterial culture;
[0060] S2.3: Mix lactic acid bacteria and bifidobacteria in a mass ratio of 5:2 to obtain a compound bacterial agent. Add donkey-hide gelatin, red algae polysaccharide and ferric sulfate to the Angelica sinensis aqueous extract. The amount of donkey-hide gelatin, red algae polysaccharide and ferric sulfate added is 5g donkey-hide gelatin, 3g jujube polysaccharide and 0.1g ferric sulfate per 100mL of Angelica sinensis aqueous extract to obtain the aqueous extract base liquid. Add the compound bacterial agent to the aqueous extract base liquid and culture. The amount of compound bacterial agent added is 5% of the system mass. Then, culture at 38℃ with shaking for 62h. Centrifuge the product at 8100r / min for 15min at 4℃ to remove the precipitate and obtain the Angelica sinensis extract fermentation broth.
[0061] S3: Preparation of Angelica sinensis blood-tonifying oral liquid
[0062] S3.1: Add the fermentation broth of Angelica sinensis extract to twice the amount of 85-95% ethanol aqueous solution and precipitate for 48 hours to remove protein and polysaccharide impurities. Concentrate the supernatant to one-fifth of the original volume at 50℃ and 0.1-0.2MPa to obtain Angelica sinensis fermentation concentrate.
[0063] S3.2: Sterilize the Angelica sinensis fermentation concentrate at 105℃ for 5 min, then add 0.1% sodium alginate by weight at 65℃, stir at 250 r / min for 25 min, then let it stand and cool to 50℃, add 1.5% β-cyclodextrin by weight, then start stirring, add 3% xylitol and 0.02% glycine ferrous chelate Fe(C2H4NO2)2 at 60 r / min to obtain the oral liquid stock solution, degas at -0.08 MPa and 45℃ for 20 min to eliminate foam and dissolved oxygen, then fill with nitrogen and bottle to obtain Angelica sinensis blood-tonifying oral liquid.
[0064] Comparative Example 1:
[0065] Compared with Example 1, the difference of Comparative Example 1 is that no lactic acid bacteria liquid is added in step S2.3, while the other steps remain unchanged, and it is referred to as Comparative Example 1.
[0066] Comparative Example 2:
[0067] Compared with Example 1, Comparative Example 2 differs in that Bifidobacterium bacterial solution is not added in step S2.3, while the other steps remain unchanged, and is referred to as Comparative Example 2.
[0068] Comparative Example 3:
[0069] Compared with Example 1, Comparative Example 3 differs in that sodium alginate is not added in step S3.2, while the other steps remain unchanged, and is referred to as Comparative Example 3.
[0070] Comparative Example 4:
[0071] Compared with Example 1, Comparative Example 4 differs in that xylitol is not added in step S2.3, while the other steps remain unchanged, and is referred to as Comparative Example 4.
[0072] The contents of total polysaccharides, ferulic acid, and angelica polysaccharides in Examples 1-3 and Comparative Examples 1-4 are shown in Table 1.
[0073] Table 1
[0074] Content (mg / mL) Total polysaccharides Angelica polysaccharides ferulic acid Example 1 176 112 0.12 Example 2 175 110 0.12 Example 3 173 109 0.11 Comparative Example 1 142 87 0.08 Comparative Example 2 141 86 0.07 Comparative Example 3 138 82 0.05 Comparative Example 4 139 81 0.05
[0075] As shown in Table 1, the total polysaccharide, angelica polysaccharide, and ferulic acid contents of Comparative Examples 1 and 2 were significantly lower than those of the Example. The synergistic fermentation of lactic acid bacteria and bifidobacteria was the core factor in enhancing the release of polysaccharides and ferulic acid. Comparative Example 3 had the lowest active ingredient content, indicating that sodium alginate, as a microcapsule wall material, can protect active ingredients such as polysaccharides and ferulic acid in a liquid environment. The data of Comparative Example 4 were similar to those of Comparative Example 3, suggesting that the lack of xylitol also led to a significant decrease in activity, indicating that xylitol also has protective capabilities, and the combination of the two provides better protection for active ingredients.
[0076] It should be understood that those skilled in the art can make improvements or modifications based on the above description, and all such improvements and modifications should fall within the protection scope of the appended claims. Parts not described in detail in this specification are prior art known to those skilled in the art.
Claims
1. A preparation process for an Angelica sinensis blood-tonifying oral liquid, characterized in that, Includes the following steps: S1: Preparation of Angelica sinensis aqueous extract Angelica sinensis was frozen, pulverized, and sieved. Then it was added to pure water and decocted twice. The filtrates from the two decoctions were combined to obtain Angelica sinensis aqueous extract. S2: Preparation of Angelica sinensis fermentation broth Lactic acid bacteria were inoculated into the culture medium, and then cysteine and manganese sulfate were added to obtain a lactic acid bacteria culture. Bifidobacterium was inoculated into the culture medium, and then Angelica sinensis water extract was added to obtain a Bifidobacterium culture. The lactic acid bacteria culture and Bifidobacterium culture were mixed to obtain a compound bacterial agent. Donkey-hide gelatin, red algae polysaccharide and ferric sulfate were added to the Angelica sinensis water extract to obtain a water extract base liquid. The compound bacterial agent was added to the water extract base liquid and cultured to obtain the Angelica sinensis extract fermentation broth. S3: Preparation of Angelica sinensis blood-tonifying oral liquid Angelica extract fermentation broth was added to an ethanol-water solution for precipitation to remove protein and polysaccharide impurities. The supernatant was concentrated to obtain Angelica fermentation concentrate. The Angelica fermentation concentrate was sterilized, and sodium alginate was added. The mixture was stirred and cooled, and β-cyclodextrin was added. The mixture was stirred, and xylitol and glycine ferrous chelate were added to obtain oral liquid stock solution. The solution was degassed to eliminate foam and dissolved oxygen, and then filled with nitrogen to obtain Angelica blood-tonifying oral liquid.
2. The preparation process of the Angelica sinensis blood-tonifying oral liquid according to claim 1, characterized in that, S1 The preparation of Angelica sinensis aqueous extract includes the following steps: Angelica sinensis was frozen at -20 to -15°C for 3-4 hours, then pulverized and passed through a 300-mesh sieve. It was then added to purified water at 10 to 15°C with a material-to-liquid ratio of 1g:(8-10)mL and soaked for 8-12 hours. The mixture was then heated to 95 to 100°C and decocted until it was reduced to one-fifth of its original volume. The mixture was filtered to obtain a primary filtrate. The residue was mixed with purified water at a material-to-liquid ratio of 1g:(6-8)mL and decocted again at 95 to 100°C until it was reduced to one-fifth of its original volume. The mixture was then filtered to obtain a secondary filtrate. The primary and secondary filtrates were mixed and stirred for 2-3 minutes to obtain an aqueous extract of Angelica sinensis.
3. The preparation process of the Angelica sinensis blood-tonifying oral liquid according to claim 2, characterized in that, The preparation of Angelica sinensis fermentation broth using S2 includes the following steps: S2.1: Inoculate lactic acid bacteria into the culture medium, then add 0.1-0.2% cysteine and 0.05-0.08% manganese sulfate by weight of the system. Shake and culture at 37-38℃ for 24-25 hours at a speed of 200-250 r / min to obtain lactic acid bacteria culture. S2.2: Inoculate Bifidobacterium into the culture medium, then add 5-6% of Angelica sinensis water extract by weight of the system, adjust the pH to 5.5-6, and culture at 37-38℃ with shaking at 200-250 r / min for 24-25 h to obtain Bifidobacterium bacterial culture; S2.3: Mix lactic acid bacteria and bifidobacteria in a mass ratio of 5:(2-3) to obtain a compound bacterial agent. Add donkey-hide gelatin, red algae polysaccharide and ferric sulfate to the Angelica sinensis water extract to obtain a water extract base liquid. Add the compound bacterial agent to the water extract base liquid for cultivation. The amount of compound bacterial agent added is 5-8% of the system mass. Then, shake and culture at 37-38℃ for 60-62h. Centrifuge the product at 4℃ at a speed of 8000-8100r / min for 10-15min to remove the precipitate and obtain the Angelica sinensis extract fermentation broth.
4. The preparation process of the Angelica sinensis blood-tonifying oral liquid according to claim 3, characterized in that, The preparation of Angelica sinensis blood-tonifying oral liquid by S3 includes the following steps: S3.1: Add the fermentation broth of Angelica sinensis extract to 2-4 times the amount of ethanol aqueous solution and precipitate for 45-48 hours to remove protein and polysaccharide impurities. Concentrate the supernatant to one-fifth of the original volume at 45-50℃ and 0.1-0.2MPa to obtain Angelica sinensis fermentation concentrate. S3.2: Sterilize the Angelica sinensis fermentation concentrate at 100-105℃ for 3-5 min, then add 0.1-0.3% sodium alginate by weight at 60-65℃, stir at 200-250 r / min for 20-25 min, then let it stand and cool to 45-50℃, add 1.5-2.5% β-cyclodextrin by weight, then start stirring, add 3-5% xylitol and 0.02-0.05% glycine ferrous chelate at 50-60 r / min to obtain the oral liquid stock solution, degas at -0.08 MPa and 40-45℃ for 15-20 min to eliminate foam and dissolved oxygen, then fill with nitrogen and bottle to obtain Angelica sinensis blood-tonifying oral liquid.
5. The preparation process of the Angelica sinensis blood-tonifying oral liquid according to claim 3, characterized in that, The culture medium is prepared by mixing 10-12 g / L peptone, 3-4 g / L beef extract, 5-6 g / L glucose and 5-7 g / L sodium chloride, and adjusting the pH of the system to 7-7.5 to obtain the culture medium.
6. The preparation process of the Angelica sinensis blood-tonifying oral liquid according to claim 3, characterized in that, The dosage of donkey-hide gelatin, red algae polysaccharide, and ferric sulfate is 5-6g of donkey-hide gelatin, 3-4g of red date polysaccharide, and 0.1-0.2g of ferric sulfate per 100mL of Angelica sinensis aqueous extract.
7. The preparation process of the Angelica sinensis blood-tonifying oral liquid according to claim 4, characterized in that, The concentration of the aqueous ethanol solution is 85-95%.
8. The preparation process of the Angelica sinensis blood-tonifying oral liquid according to claim 4, characterized in that, The glycine ferrous chelate is specifically Fe(C2H4NO2)2.
9. An oral liquid for replenishing blood with Angelica sinensis, characterized in that, It is prepared by the preparation process of Angelica sinensis blood-tonifying oral liquid according to any one of claims 1-8.