342 results about "Absolute quantification" patented technology

The invention provides reagents, kits and methods for detecting and/or quantifying proteins in complex mixtures, such as a cell lysate. The methods can be used in high throughput assays to profile cellular proteomes. In one aspect, the invention provides a peptide internal standard labeled with a stable isotope and corresponding in amino acid sequence to the amino acid sequence of a subsequence of a target polypeptide. In another aspect, the peptide internal standard is labeled at a modified amino acid residue and is used to determine the presence of, and/or quantitate the amount of a particular modified form of a protein.

The present invention concerns a method for the quantification of a target nucleic acid in a sample comprising the following steps: (i) determination of the amplification efficiency of the target nucleic acid under defined amplification conditions, (ii) amplification of the target nucleic acid contained in the sample under the same defined reaction conditions, (iii) measuring the amplification in real-time, (iv) quantification of the original amount of target nucleic acid in the sample by correction of the original amount derived from step (iii) with the aid of the determined amplification efficiency. The efficiency correction of PCR reactions according to the invention for the quantification of nucleic acids can be used for absolute quantification with the aid of an external or internal standard as well as for relative quantification compared to the expression of housekeeping genes.

The invention relates to inspection and quarantine systems for biomedicine, agriculture, food hygiene and the like and particularly relates to an absolute quantification type digital nucleic acid analytic system based on an efficient liquid drop microreactor. The absolute quantification type digital nucleic acid analytic system is mainly used for detecting the molecular structure of nucleic acid so as to analyze attributes of organisms and belongs to the fields of biological and medical detection. The absolute quantification type digital nucleic acid analytic system comprises a confocal fluorescence image collecting system, a micro-fluidic chip, a temperature circulating heating system, an electric loading platform, an automatic sample feeding device, a controller and a computer. The formation and PCR amplification of micro-droplets are finished by virtue of the same micro-fluidic chip, namely the micro-fluidic chip has a droplet formation function and a PCR amplification (microreactor) function. According to the absolute quantification type digital nucleic acid analytic system, the complexity of the absolute quantification type nucleic acid analysis process is reduced, the nucleic acid analysis process is finished in a one-button manner, and culture and bacteria-proliferating processes are omitted, so that the rapid detection is realized, the sensitivity of the absolute quantification type nucleic acid detection is greatly improved, meanwhile, the sample consumption of the absolute quantification type nucleic acid analysis is substantially reduced, and the high throughput detection is realized.

A method for absolute protein or peptide quantitation by mass spectroscopy. A sample containing a protein or peptide of interest is prepared for mass spectroscopy analysis. The sample is subjected to mass spectroscopy analysis at low resolution whereby a single additive mass spectroscopy peak is obtained, then is subjected to high resolution mass spectroscopy analysis whereby a plurality of mass spectroscopy peaks are obtained. The intensity of each of the plurality of mass spectroscopy peaks is quantitated either by comparison to an internal standard set, or by using a standard curve generated for each isotopologue set. Quantitation using a standard curve enhances quantitation across a dynamic range of analyte.

In various aspects, the present teachings provide systems, methods, assays and kits for the absolute quantitation of protein expression. In various aspects, the present teachings provide methods of determining the concentration of one or more proteins of interest in one or more samples of interest. In various aspects, the present teachings provide methods of determining the absolute concentration of one or more isoforms of a protein using standard samples of signature protein fragments and parent-daughter ion transition monitoring (PDITM). In various embodiments, the absolute concentration of multiple isoforms of a biomolecule in a sample, multiple proteins in a biological process, a combination of multiple samples, or combinations thereof, can be determined in a multiplex fashion using the present teachings. In various aspects, provided are methods of assessing the response of a biological system to a chemical agent.

The application relates to the examining field of gene mutation and particularly relates to a PCR micro droplet kit for examining human EGFR gene mutation. The kit includes a nucleic acid amplification reagent; a reference substance and micro droplet PCR generated oil. The nucleic acid amplification reagent includes a primer and a probe for examining the No. 18 extron mutant, a primer and a probe for examining the No. 19 extron mutant, a primer and a probe for examining the No. 20 extron mutant, and a primer and a probe for examining the No. 21 extron mutant. The present application can be used for examining the seven species of the No. 18, 19, 20, and 21 extron mutants for the EGFR genes in the DNAs extracted from the paraffin-embedded pathology tissue slices and the peripheral blood dissociate DNAs. The present application provides a reference for the patients to choose the tumor molecularly targeted drug remedy and monitor. The present invention has absolute ration, high specificity, accuracy and high sensitivity.

The invention relates to a low abundant protein absolute quantification method based on a digital immunoassay technology. The method comprises the following steps: carrying out an immunoreaction on captured beads, target antigens and detected particles so as to form an immune complex, eluting the detected particles in the immune complex, collecting and concentrating the eluant, transferring the concentrated solution to a micro-fluidic particle counting chip, depositing and fixing the detected particles on the micro-fluidic particle counting chip after a solvent in the concentrated solution iscompletely volatilized, performing imaging recording on all the detected particles fixed on the micro-fluidic particle counting chip, and counting the quantity of the detected particles on the micro-fluidic particle counting chip, thereby obtaining the molecular number of the target protein. The method disclosed by the invention has the advantages that ultra-sensitive and absolute quantification analysis of disease-related proteins in clinical samples can be realized, any standard substance is not needed, a standard curve does not need to be drawn, and the method has the advantages of being wide in application range, high in detection sensitivity, high in analytical result accuracy, excellent in precision degree and high in throughput.

In various aspects, the present teachings provide systems, methods, assays and kits for the absolute quantitation of protein expression. In various aspects, the present teachings provide methods of determining the concentration of one or more proteins of interest in one or more samples of interest. In various aspects, the present teachings provide methods of determining the absolute concentration of one or more isoforms of a protein using standard samples of signature protein fragments and parent-daughter ion transition monitoring (PDITM). In various embodiments, the absolute concentration of multiple isoforms of a biomolecule in a sample, multiple proteins in a biological process, a combination of multiple samples, or combinations thereof, can be determined in a multiplex fashion using the present teachings. In various aspects, provided are methods of assessing the response of a biological system to a chemical agent.

The invention relates to a proteome label-free quantification method combining tandem mass spectrometry with machine learning algorithm, which is used for absolute and relative quantitative analysis of a proteome level. The method firstly analyzes an enzymolytic peptide fragment mixture of a proteome actual sample for establishing a training data set and an enzymolytic peptide fragment mixture of a proteome sample to be analyzed by using a liquid chromatography-tandem mass spectrometry system. The total amount of the samples can be obtained by cell counting or protein concentration determination, and the absolute amount of each protein can be calculated according to the percents and sample total amount calculated in the last step. The absolute amounts of the same protein in different samples are compared to obtain relative quantitative information of the protein in different samples. The method has good accuracy in both absolute quantification and relative quantification.

The invention discloses a digital PCR-based novel coronavirus nucleic acid quantitative detection kit and an application. The reaction total system of the digital PCR-based novel coronavirus nucleic acid quantitative detection kit has 20 ul, comprising 10ul of 2x One-Step RT-ddPCR Supermix, 0.8ul of 25mM manganese acetate solution, 5ul of to-be-detected sample RNA, 1ul of ORFlab gene primer probeworking solution, 1ul of N gene primer probe working solution, 1ul of RPP30 gene primer probe working solution and 1.2ul of Nuclease-Free Water, wherein 5ul of negative control RNA extraction solutionand 5ul of positive control RNA extraction solution are adopted to replace the to-be-detected sample RNA in a negative control reaction system and a positive control reaction system respectively. Based on the innovative RNA one-step reverse transcription microdroplet type digital PCR technology, nucleic acid absolute quantification is carried out specific to highly conservative ORFlab gene and Ngene in the novel coronavirus (2019-nCoV) genome, so that the detection accuracy is improved, and the kit can be used for clinical assisted diagnosis and viral load analysis of novel coronavirus (2019-nCoV) infection, and has a wide clinical application value.

The invention provides a kit and method for detecting the mutation of an EGFR gene and particularly discloses a method, a primer and a probe for detecting the mutation of the EGFR gene, a kit including prime probe mixing liquid, a primer and a probe for detecting the mutation of a C797S locus, a primer and a probe for detecting the mutation of a T790M locus, a primer and a probe for detecting themutation of a 19del locus and a primer and a probe for detecting the mutation of a L858 locus. According to the method provided by the invention, 0.1% mutation rate can be detected based on a digitalPCR platform; and the method has the advantages that the optimization process is simple, much detected mutation types can be detected, the absolute quantification is realized, the sensitivity is high,samples are easily acquired, and the like.

The invention discloses a typing detecting kit and method for detecting the digital PCR absolute quantification of HBV-B/C. The kit comprises a DNA extraction solution, a digital PCR reaction buffer solution A, a digital PCR reaction buffer solution B, an HBV-B virus gene positive quality control, an HBV-C virus gene positive quality control, a negative quality control and an internal standard solution. The method includes the following steps of firstly, processing a to-be-detected sample; secondly, processing a quality control product; thirdly, preparing a digital PCR reaction mixed solution; fourthly, generating a micro-reaction liquid drop, and conducting digital PCR reaction amplification; fifthly, reading a fluorescent signal, analyzing an HBV type and calculating the number of copying times. By means of the digital PCR technology and the dual-color florescent probe, two subtypes of HBV are detected at the same time; without depending on an external standard object or a standard curve, the kit and method are easy and convenient to operate and can directly detect the HBV precise absolute quantification.

The present application provides methods and devices for absolute quantification of polymerase chain reaction target nucleic acids. In particular, the methods and devices of the present application provide for splitting a nucleic acid sample to be analyzed into small, isolated volumes, conducting the method of polymerase chain reaction (PCR) on said volumes, detecting PCR amplification products, analyzing said detected PCR amplification products, performing absolute quantification of the PCR target and presenting said quantification results.

The invention relates to a quantitative detection method for transgene protein CP4-EPSPS in a plant. The quantitative detection method comprises: extracting protein from a plant to obtain a crude protein extraction solution; and carrying out digestion on the crude protein extraction solution, adopting three 18O-labeled CP4-EPSPS specific peptide segments as internal standards, adopting UPLC/ESI-QQQ MS detection, and carrying out quantification on CP4-EPSPS in a peptide segment level, wherein the three specific peptide segment sequences are ITGLLEGEDVINTGK, SFMFGGLASGETR and LAGGEDVADLR. According to the present invention, the method has high sensitivity, and absolute CP4-EPSPS quantification can be achieved through combination of an isotope dilution method and an MRM strategy.

The invention provides a HBV nucleic acid quantitative detection system based on a micro-droplet digital PCR technology, the HBV nucleic acid quantitative detection system comprises an upstream primerand a downstream primer for amplifying hepatitis B virus DNA, a specific probe for detecting the hepatitis B virus DNA; a non-infected linearizing double-stranded plasmid quality control standard product and a probe for detecting the quality control standard product; and a buffer system comprising deoxyribonucleoside triphosphates dATP, dCTP, dGTP, and dUTP, and UNG. The HBV nucleic acid quantitative detection system is based on the micro-droplet digital PCR technology to realize the accurate absolute quantification of HBV DNA, faces the clinical actual demand, and has an important application prospect.

The invention provides a method for detecting the number of arbuscular mycorrhizal fungi in wheat rhizosphere soil based on real-time fluorescence quantification PCR. The method includes the steps that firstly, sample total DNA of wheat rhizosphere soil is extracted and serves as a template of PCR amplification; then, amplification is conducted with SEQ ID No.1 and SEQ ID No. 2 as primers, and specific fragments of 129 bp are obtained; after bond and conversion are conducted, qPCR amplification is conducted. By means of the method, arbuscular mycorrhiza is authenticated, absolute quantification can be conducted on the arbuscular mycorrhiza accurately, the copy number of the arbuscular mycorrhizal fungi in all periods of duration of transgenic wheat is obtained, and in the growth and development stages of wheat, the arbuscular mycorrhiza copy number tends to be gradually increased as a whole. The rapid, convenient and precise authentication method is built, a theoretical and test basis is provided for subsequent tests, great significance is provided for further evaluation of safety of transgenic crops, and compared with an existing method, the method is high in sensitivity, high in specificity, good in repeatability, convenient to operate and visual in result.

The invention discloses a method for detecting aldehydes in rice. According to the method, the method is realized by employing a headspace derived solid phase micro-extraction-gas chromatography-mass spectrometry method. The method comprises the following steps of: grinding a rice sample, adding the detected sample into a headspace bottle, introducing protective gas, inserting an extraction head into the headspace bottle filled with derivative liquid for deriving for 20 minutes, and inserting the extraction head into a headspace sample introduction bottle, wherein the extraction temperature is 40 DEG C, and the extraction time is 80 minutes; detecting in a gas chromatograph-mass spectrometer. The fineness and headspace derivatization conditions of the ground rice particles are optimized, so that newly generated aldehydes in the analysis process are avoided, and the accuracy of an experimental result is improved. According to the detection method, five steps of adsorbing derivative liquid of the extraction head, deriving and extracting the aldehydes, performing sample introduction, separating and measuring can be sequentially finished in a process, automation is realized, the operation is simple, the detection efficiency and accuracy are improved, and absolute quantification of nine aldehydes in rice is realized.

The invention discloses a novel coronavirus 2019-nCoV nucleic acid kit and a virus nucleic acid acquisition method. The method comprises the following steps: according to novel coronavirus 2019-nCoV ORF1ab and an N gene as amplification target areas, designing specific primers and fluorescent probes for detecting novel coronavirus 2019-nCoV nucleic acid in samples; and performing Digital PCR (polymerase chain reaction) absolute quantification on 2019-nCoV nucleic acid RNA (ribonucleic acid) by using a one-step method by using a prepared Super premix reaction liquid A together with Super premixreaction liquids B and C, and performing Digital PCR detection on RNA of a diluted positive virus sample. The kit and the method have the beneficial effects that by using the novel virus nucleic acidacquisition method, and together with a Digital PCR probe method, the nucleic acid positive detection rate of infection of the novel coronavirus 2019-nCoV can be increased, the process is ensured tobe specified and standardized, the influence of artificial non-standard operation upon detection results can be reduced, result reliability can be ensured, and meanwhile, false negative results causedby detection of sputum and throat swab sampling together with real-time fluorescent PCR methods can be avoided.

The invention discloses an absolute quantitative analysis method of lysophosphatidyl choline based on an HPLC-MS/MS detection platform. The absolute quantitative analysis method establishes the HPLC-MS/MS detection platform, takes reserpine as an internal standard to draw the standard curves of LPC 14:0-LPC 18:0 serial substances, verifies the linearity, precision and accuracy of the detection platform, and detects the matrix effect of blood serum. Proven by verification, the HPLC-MS/MS detection platform established by the invention has higher stability and accuracy in detection of the LPC substances in the human blood serum, meets the clinical requirements and is suitable for clinical popularization.