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2 results about "Amplified fragment length polymorphism" patented technology
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AFLP-PCR or just AFLP is a PCR-based tool used in genetics research, DNA fingerprinting, and in the practice of genetic engineering. Developed in the early 1990s by Keygene, AFLP uses restriction enzymes to digest genomic DNA, followed by ligation of adaptors to the sticky ends of the restriction fragments. A subset of the restriction fragments is then selected to be amplified. This selection is achieved by using primers complementary to the adaptor sequence, the restriction site sequence and a few nucleotides inside the restriction site fragments (as described in detail below). The amplified fragments are separated and visualized on denaturing on agarose gel electrophoresis , either through autoradiography or fluorescence methodologies, or via automated capillary sequencing instruments.
PCR primer for identifying offsprings of interspecific cross of portunus trituberculatus, and interspecific cross method and application thereof
PendingCN122405844APortunus sanguinolentusInterspecific hybridization
This invention discloses PCR primers, interspecific hybridization methods, and applications for identifying interspecific hybrid offspring of swimming crabs, belonging to the field of aquatic animal genetic engineering technology. The PCR primers of this invention include an upstream primer as shown in SEQ ID NO.1 and a downstream primer as shown in SEQ ID NO.2. When using these PCR primers for PCR amplification, a 786 bp band indicates *Portunus trituberculatus*; a 632 bp band indicates *Portunus cerana*; and a 963 bp band indicates *Portunus rubrum*. The hybrid offspring simultaneously exhibit two specific amplification bands, thus achieving rapid and accurate identification of the hybrid offspring. This invention establishes for the first time a method for identifying hybrid offspring based on PCR amplification fragment length polymorphism, which has advantages such as simple operation, low cost, high accuracy, and suitability for large-scale detection. It can be applied to the fields of swimming crab germplasm innovation, new variety breeding, and genetic improvement.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Molecular marker related to high lysine character of corn as well as primer group and application of molecular marker
PendingCN121496092AMicrobiological testing/measurementFood processingGenome resequencingGel electrophoresis
The invention discloses a molecular marker related to the high lysine character of corn as well as a primer group and application of the molecular marker, and belongs to the technical field of plant molecular breeding. On the basis of whole genome re-sequencing comparison, an InDel molecular marker closely linked with a corn high-lysine key gene is found, and the marker is located in a corn chromosome 7. The invention provides a specific primer group for detecting the marker, PCR (Polymerase Chain Reaction) amplification is carried out by utilizing the primer group, and identification can be carried out through agarose gel electrophoresis according to the length polymorphism of an amplified fragment: an individual amplified with a 835bp band is a high-lysine genotype, an individual amplified with a 610bp band is a common genotype, and an individual amplified with the 610bp band is a high-lysine genotype. And the individual with the 835bp band and the 610bp band simultaneously appearing is a heterozygous genotype. Therefore, the invention provides an efficient and reliable technical tool for molecular marker-assisted selective breeding of high-lysine corn.
Owner:RICE & SORGHUM INST SICHUAN ACAD OF AGRI SCI