4 results about "Amplified fragment length polymorphism" patented technology

This invention discloses PCR primers, interspecific hybridization methods, and applications for identifying interspecific hybrid offspring of swimming crabs, belonging to the field of aquatic animal genetic engineering technology. The PCR primers of this invention include an upstream primer as shown in SEQ ID NO.1 and a downstream primer as shown in SEQ ID NO.2. When using these PCR primers for PCR amplification, a 786 bp band indicates *Portunus trituberculatus*; a 632 bp band indicates *Portunus cerana*; and a 963 bp band indicates *Portunus rubrum*. The hybrid offspring simultaneously exhibit two specific amplification bands, thus achieving rapid and accurate identification of the hybrid offspring. This invention establishes for the first time a method for identifying hybrid offspring based on PCR amplification fragment length polymorphism, which has advantages such as simple operation, low cost, high accuracy, and suitability for large-scale detection. It can be applied to the fields of swimming crab germplasm innovation, new variety breeding, and genetic improvement.

The invention discloses a molecular marker related to the high lysine character of corn as well as a primer group and application of the molecular marker, and belongs to the technical field of plant molecular breeding. On the basis of whole genome re-sequencing comparison, an InDel molecular marker closely linked with a corn high-lysine key gene is found, and the marker is located in a corn chromosome 7. The invention provides a specific primer group for detecting the marker, PCR (Polymerase Chain Reaction) amplification is carried out by utilizing the primer group, and identification can be carried out through agarose gel electrophoresis according to the length polymorphism of an amplified fragment: an individual amplified with a 835bp band is a high-lysine genotype, an individual amplified with a 610bp band is a common genotype, and an individual amplified with the 610bp band is a high-lysine genotype. And the individual with the 835bp band and the 610bp band simultaneously appearing is a heterozygous genotype. Therefore, the invention provides an efficient and reliable technical tool for molecular marker-assisted selective breeding of high-lysine corn.

According to the molecular marker method for rapidly identifying the southern and northern kelp culture population, the nucleotide sequence of the specific molecular marker of the northern strain is SEQ ID NO: 1, and the nucleotide sequence of the specific molecular marker of the southern strain is SEQ ID NO: 2. Compared with amplified fragment length polymorphism, restriction site amplification polymorphism, microsatellite DNA marking and the like, the method is more convenient, only simple PCR amplification needs to be carried out, distinguishing can be carried out according to sequencing data, and tedious experiment steps are omitted. The molecular marker provided by the invention can be quickly identified only through simple PCR amplification and next-generation sequencing, so that the detection cost is relatively low. The development of the specific molecular marker not only can avoid seedling mixing in production, but also can lay a foundation for evaluation and identification of subsequent new strains.

The invention relates to the technical field of aquaculture, and particularly discloses a southern sea cucumber artificial breeding method, which comprises the following steps: S1, collecting southern sea cucumber population samples, and carrying out genetic analysis on the samples by utilizing SSR (Simple Sequence Repeat) marking, SNP (Single Nucleotide Polymorphism) marking, AFLP (Amplified Fragment Length Polymorphism) marking and RAPD (Random Amplified Polymorphic DNA) marking technologies, the parent sea cucumbers which are high in growth speed, strong in stress resistance, full in body shape, delicious in meat quality and high in reproductive capacity are screened out; s2, performing health examination and pathogen detection on the screened parent sea cucumbers; according to the method, by adopting advanced genetic marker technologies such as SSR markers, SNP markers, AFLP markers and RAPD markers, parents with excellent characters such as high growth speed, strong stress resistance, full body type, delicious meat quality and high reproductive capacity are rapidly screened, parent screening is performed through a strict statistical method and a genetic algorithm, the accuracy and reliability of screening results are ensured, and the method is suitable for large-scale popularization and application. Through accurate genetic analysis and parent screening, unnecessary use of parent sea cucumbers is reduced, breeding resources are optimized, and the breeding cost is reduced.