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Method for identifying Qianshan No.2, new variety of Erigeron Breviscapus (Vant.)Hand.-Mazz. by utilizing AFLP (amplified fragment length polymorphism)-CE (capillary electrophoresis) technique

A technology of capillary electrophoresis and Dengzhanhua, which is used in biochemical equipment and methods, determination/inspection of microorganisms, material analysis by electromagnetic means, etc., to achieve the effects of easy standardized analysis, reduction of errors, and improvement of reliability.

Inactive Publication Date: 2014-01-01
红河千山生物工程有限公司
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Problems solved by technology

But so far, no AFLP molecular marker technology has been used for molecular identification of breviscapine, nor has AFLP-capillary electrophoresis technology been used for molecular identification of breviscapine in different regions and molecular identification of a new species of breviscapine Qianshan No. 2

Method used

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  • Method for identifying Qianshan No.2, new variety of Erigeron Breviscapus (Vant.)Hand.-Mazz. by utilizing AFLP (amplified fragment length polymorphism)-CE (capillary electrophoresis) technique
  • Method for identifying Qianshan No.2, new variety of Erigeron Breviscapus (Vant.)Hand.-Mazz. by utilizing AFLP (amplified fragment length polymorphism)-CE (capillary electrophoresis) technique
  • Method for identifying Qianshan No.2, new variety of Erigeron Breviscapus (Vant.)Hand.-Mazz. by utilizing AFLP (amplified fragment length polymorphism)-CE (capillary electrophoresis) technique

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Embodiment

[0038] Main experimental instruments:

[0039] CEQ8000 capillary electrophoresis system (Beckman Coulter); desktop refrigerated centrifuge (Beckman Coulter); PCR instrument (MJ Research PTC200-well thermal cycler); ultraviolet spectrophotometer (UV-2550, SHIMADZU company), gel imaging system.

[0040] The method of the present invention adopts conventional AFLP-capillary electrophoresis technology except for the following special instructions. Various reagents used in each example were purchased from Shanghai Sangong Hebao Biological Co., Ltd.

[0041] The molecular identification method of Qianshan No. 2, a kind of breviscapine new variety of the present invention, is carried out according to the following steps:

[0042] (1) Collection of samples: A total of 5 samples were collected from Luxi County (No. Five species of Erigeron breviscapi from four areas in Shilin County, Kunming City, with Qianshan No. Differential samples, 15 plants per sample. The five species of bre...

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Abstract

The invention discloses a method for identifying Qianshan No.2, a new variety of Erigeron Breviscapus (Vant.)Hand.-Mazz. by utilizing AFLP (amplified fragment length polymorphism)-CE (capillary electrophoresis) technique, belonging to the technical field of molecular markers. The method comprises the steps of extraction of sample DNA, double digestion of DNA samples and linking of double digestion products, pre-amplification of linking products, selective amplification, AFLP-CE detection and result analysis and identification. By adopting the method, an AFLP-CE separation diagram of Qianshan No.2, a new variety of Erigeron Breviscapus (Vant.)Hand.-Mazz. is successfully established, whether the samples are Qianshan No.2, a new variety of Erigeron Breviscapus (Vant.)Hand.-Mazz. can be easily and accurately identified and scientific bases are provided for protecting Qianshan No.2, a new variety of Erigeron Breviscapus (Vant.)Hand.-Mazz..

Description

technical field [0001] The invention relates to the technical field of molecular markers, in particular to random amplified fragment length polymorphism (AFLP)-capillary electrophoresis technology and molecular identification of the new species of Erigeron breviscapus Qianshan No. 2. Background technique [0002] Breviscapus [Erigeron breviscapus (Vant.) Hand.-Mazz.] is a perennial plant of the genus Asteraceae. It is a good natural medicine for treating cardiovascular and cerebrovascular diseases. Its total flavonoids, breviscapus, and caffeoylquinic acid There are 4 types of injections developed with similar compounds as active ingredients, and it is one of the most potential medicinal plants in Yunnan Province. [0003] Scutellarin is one of the main functional components of scutellarin. The content of scutellarin determines the curative effect of medicinal materials, and is also an important criterion for identifying the quality of medicinal materials. Due to the incre...

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Application Information

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IPC IPC(8): C12Q1/68G01N27/447
CPCC12Q1/686C12Q2535/138C12Q2565/125C12Q2531/113
Inventor 张广辉杨生超张薇杨建文吴道聪龙光强岳艳玲
Owner 红河千山生物工程有限公司
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