15 results about "Erigeron breviscapus" patented technology

The invention discloses a preparation method and a quality control method of a traditional Chinese medicine composition for stroke and middle meridian syndromes caused by extravasated blood blocking collaterals, and relates to the field of traditional Chinese medicines. The preparation method comprises the following steps: extracting salvia miltiorrhiza by adopting a composite enzymolysis-two-phase low-temperature extraction method, extracting erigeron breviscapus by adopting citric acid-ethanol extraction, extracting ligusticum wallichii by adopting double-template molecular imprinting-stir-frying with bran, extracting kudzu vine root by adopting a microwave method, adding auxiliary materials including hydroxypropyl methyl cellulose, sodium carboxymethyl cellulose and beta-cyclodextrin, drying by adopting a centrifugal spray dryer, and packaging by adopting a packaging machine. The Maillard reaction is regulated and controlled. The method has the advantages of efficient extraction, component optimization, preparation stabilization, drug effect enhancement, adverse reaction rate reduction, quality controllability and the like.

The invention relates to a KASP molecular marker associated with scutellarin of erigeron breviscapus and application of the KASP molecular marker, and belongs to the technical field of genetic engineering. The primers of the KASP molecular marker associated with the scutellarin of the erigeron breviscapus comprise a forward primer 1, a forward primer 2 and a common reverse primer; the nucleotide sequence of the forward primer 1 is as shown in SEQ ID NO. 1; the nucleotide sequence of the forward primer 2 is as shown in SEQ ID NO. 2; the nucleotide sequence of the common reverse primer is as shown in SEQ ID NO.3. By utilizing the KASP technology, the SNP site and the corresponding primer combination provided by the invention not only improve the plant variety selection efficiency, but also are beneficial to shortening the breeding cycle and accelerating the cultivation of high-quality plant varieties. The markers have good genetic stability and high resolution, are suitable for high-throughput detection, and have significant application value in the field of plant breeding.

The present invention discloses a mutant of Erigeron breviscapus with enhanced chalcone synthase activity and / or selectivity, and a screening method and application thereof. p The invention relates to a recombinant plasmid for synthesizing phloretin using ‑coumaric acid as a substrate. Through structure prediction and catalytic mechanism, sites related to the catalytic activity and selectivity of EbCHS are screened, and effective mutants and effective mutation sites are obtained through experimental screening. EbCHS is compared with different CHS sequences, and screening experiments are performed to obtain effective mutants of non-conserved amino acid sites adjacent to the above-mentioned effective mutation sites. The two obtained effective mutants are combined in pairs to obtain relevant detection information. Based on the above-mentioned experimental information, a database is built and machine learning modeling is predicted. After experimental verification, EbCHS with significantly enhanced activity and selectivity in the phloretin synthesis pathway is obtained, which can be used as p ‑coumaric acid as a substrate for efficient synthesis of phloretin and reduced by-products, providing technical support for promoting the industrial production of phloretin.

The invention provides a traditional Chinese medicine preparation for cerebral arterial thrombosis and a preparation method thereof, and relates to the technical field of traditional Chinese medicine pharmacy, the traditional Chinese medicine preparation is prepared from trichosanthes kirilowii maxim, inula flower, tamarix chinensis, arisaema cum bile, calculus bovis factitius, hawksbill, erigeron breviscapus, rhodiola rosea, radix paeoniae rubra, radix curcumae, herba lycopi, campsis grandiflora, catechu and benzoin. Comprehensive animal experiment and clinical research results show that the traditional Chinese medicine preparation has a remarkable treatment effect on cerebral arterial thrombosis, mNSS scores of MCAO rats can be remarkably reduced, the cerebral infarction volume can be reduced, meanwhile, inflammatory response balance in vivo can be adjusted, and inflammation-mediated brain injury can be relieved; the preparation can also significantly reduce the NIHSS score and the traditional Chinese medicine syndrome score of the cerebral arterial thrombosis patient, increase the BI score, effectively regulate the serum inflammatory factor balance, and significantly improve the total effective rate of clinical treatment. In conclusion, the preparation plays the roles of nerve protection, anti-inflammation and function recovery promotion through multiple targets, and a safe and effective new scheme is provided for treatment of cerebral arterial thrombosis.

This invention provides a method for extracting active ingredients from *Erigeron breviscapus*, belonging to the field of pharmaceutical technology. Based on the differences in the physicochemical properties of the compounds, this invention first achieves preliminary separation of caffeic acid esters and scutellarin, followed by further purification and separation based on the properties of the two types of substances. Specifically, this invention uses ethanol-water solutions of different concentrations to extract caffeic acid esters stepwise, and uses macroporous and microporous resins for preliminary separation of the caffeic acid esters. Scutellarin is then preliminarily separated through acid-base treatment. Finally, high-purity scutellarin, 3,5-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, and scutellarin are obtained through reversed-phase industrial chromatography. This invention achieves the extraction and separation of multiple active ingredients from *Erigeron breviscapus*, and all solvents used in the extraction and separation process are recyclable, resulting in low cost.

The invention discloses a traditional Chinese medicine injection for treating cardiovascular and cerebrovascular diseases and a preparation method thereof, and relates to the technical field of traditional Chinese medicine injections.The injection is an erigeron breviscapus freeze-dried powder injection, takes an erigeron breviscapus extract as a unique active ingredient and contains pharmaceutical auxiliary materials; wherein each unit of the preparation contains 3-5g of erigeron breviscapus total flavonoids, the water content of the freeze-dried powder injection is less than or equal to 3.0%, and the pharmaceutical auxiliary materials comprise a stabilizer and a supporting agent; the stabilizer is one of sodium pyrosulfite or sodium hydrogen sulfite, and the addition amount of the stabilizer is 0.05-0.1% of the total weight of the preparation; the supporting agent is a combination of mannitol and glucose, and the addition amount of the supporting agent is 3-5% of the total weight of the preparation, according to the scheme, through purification-molding-quality control three-in-one innovation, on the premise that no high-end equipment is added, four breakthrough of safety, effectiveness, stability and economical efficiency is achieved, the upgrading requirement of the traditional Chinese medicine injection industry is completely met, and the application prospect is wide. The problem of safety of traditional Chinese medicine injections is effectively solved.

The present invention discloses a method for constructing and applying a Cas9 gene editing system for Erigeron breviscapus, comprising the following steps: selecting an Erigeron breviscapus EbPDS gene sequence and designing a target site, amplifying a target fragment using a PGTR plasmid as a template, connecting the fragment to an expression vector PV58-Cas9 by homologous recombination, infecting an Erigeron breviscapus leaf explant in a suspension infection solution containing PV58-Cas9-EbPDS Agrobacterium, and then culturing the explant in a Erigeron breviscapus co-culture medium, a delayed screening medium, and a screening medium, respectively, to obtain newly generated callus tissue. The advantages of the present invention are: (1) the gene editing system constructed with the recombinant plasmid PV58-Cas9-EbPDS is efficient and feasible; (2) the EbPDS gene is successfully applied to CRISPR / Cas9-mediated genome editing, and individuals with a specific genotype can be quickly screened out by observing whether the offspring plants show an albinism phenotype, thereby providing an important means for gene function research and gene editing breeding in Erigeron breviscapus.

This invention belongs to the field of drug detection technology, specifically relating to a detection method for *Erigeron breviscapus* (Dendrobium nobile) formula granules. These granules are made from *Erigeron breviscapus* medicinal slices through decoction, concentration, drying, and granulation. The detection method includes morphology, identification, inspection, extractives, characteristic chromatograms, and content determination. The identification method uses thin-layer chromatography; the characteristic chromatogram uses high-performance liquid chromatography, with the baicalin reference peak as the S peak, indicating 11 characteristic peaks; and the content determination measures the content of baicalin. The detection method established by this invention improves the detection requirements for *Erigeron breviscapus* medicinal materials, medicinal slices, standard decoctions, and formula granules, contributing to the establishment of a comprehensive quality control system for various *Erigeron breviscapus* products and helping to ensure the quality and clinical efficacy of *Erigeron breviscapus* formula granules.

The utility model relates to the field of erigeron breviscapus seed screening, and discloses a seed screening device for erigeron breviscapus planting, which comprises a base, the top of the base is fixedly connected with a collecting shell, a sliding assembly is arranged in the collecting shell, the sliding assembly comprises two symmetrically distributed sliding grooves formed in the two sides of the collecting shell, and the sliding grooves are communicated with the collecting shell. Four sliding grooves are formed in the bottom of the collecting shell, sliding blocks are slidably connected into the four sliding grooves, a screening assembly is arranged in the collecting shell, a motor is fixedly installed at the top of the base, and the output end of the motor is fixedly connected with a first rotating shaft. Through reciprocating motion of the first screening shell and the second screening shell, seeds can be accelerated to pass through the first screening shell and the second screening shell at the moment, the screening time is shortened, more unnecessary workload of workers is reduced, and use of the workers is greatly facilitated.

The invention discloses a traditional Chinese medicine formula for preventing and treating cerebrovascular diseases. The traditional Chinese medicine formula comprises Yulangsan, bauhinia championii, corydalis amabilis, lumbricus, astragalus membranaceus, ligusticum chuanxiong hort, cassia twig, radix paeoniae rubra, safflower, rhizoma acori graminei and erigeron breviscapus. The traditional Chinese medicine composition has good clinical effects on treating cerebral atherosclerosis, stenosis, infarction, atherosclerosis and apoplexy sequelae, hemiplegia, aphasia and the like caused by cerebral atherosclerosis, stenosis, infarction and atherosclerosis, and can gradually or basically recover the apoplexy sequelae caused by cerebral atherosclerosis, stenosis, infarction and atherosclerosis; yulangsan and bauhinia championii have the effects of relaxing tendons and activating collaterals, and corydalis amabilis, ligusticum wallichii, radix paeoniae rubra and safflower have the effects of promoting blood circulation and removing blood stasis and are The earthworm, the cassia twig, the rhizoma acori graminei and the erigeron breviscapus are used for dredging brain collaterals and are assistant and guide; the whole formula has the effects of tonifying qi, activating blood, dispersing blood stasis, dredging collaterals, inducing resuscitation and inducing resuscitation.

The invention discloses an erigeron breviscapus chalcone synthase activity and / or selectivity enhanced mutant as well as a screening method and application thereof. The construction method comprises the following steps: constructing a recombinant plasmid for synthesizing phloretin by taking p-coumaric acid as a substrate, screening out sites related to the catalytic activity and selectivity of EbCHS through structural prediction and a catalytic mechanism, and carrying out experimental screening to obtain effective mutants and effective mutation sites; comparing the EbCHS with different CHS sequences, and carrying out screening experiments to obtain effective mutants of non-conservative amino acid sites adjacent to the effective mutation sites; combining the two obtained effective mutants in pairs to obtain related detection information; according to the experimental information library building, machine learning modeling prediction and experimental verification, the EbCHS with obviously enhanced activity and selectivity in the phloretin synthesis route is obtained, the phloretin can be efficiently synthesized by taking p-coumaric acid as a substrate, byproducts are reduced, and a technical support is provided for promoting the industrial production of the phloretin.

The invention relates to an application of Erigeron breviscapus transcription factor EbbHLH80 in enhancing the salt tolerance of tobacco and a method for enhancing the salt tolerance of tobacco plants, belonging to the technical field of genetic engineering.

This invention provides a composition with menstrual regulating and blood-activating effects and its application in the preparation of external products, relating to the field of biomedical technology. This invention utilizes a composition composed of Chinese herbal raw materials including *Cephalotaxus fortunei* bark, star anise, *Erigeron breviscapus*, *Piper longum*, *Caulis thunbergii*, *Radix Achyranthis Bidentatae*, *Radix Scutellariae*, *Radix Achyranthis Bidentatae*, *Radix Panax notoginseng*, *Radix Bambusae in Taeniam*, and *Radix Achyranthis Bidentatae* to prepare a traditional Chinese medicine acupoint patch for treating or improving primary dysmenorrhea. The herbal acupoint patch can inhibit the expression of inflammatory factors, downregulate the expression level of COX-2, thereby inhibiting PGF2α, increasing the level of PGE2, and reducing uterine tissue inflammation, achieving multi-target treatment of primary dysmenorrhea. The herbal acupoint patch utilizes the power of the medicine through the acupoint, and the acupoint utilizes the properties of the medicine. The meridians are the channels, and the acupoints are the gateways. The medicinal power directly reaches the Chong and Ren meridians and the uterus, so that cold is warmed and dispelled, blood stasis is cleared and eliminated, and qi and blood flow smoothly, thus curing dysmenorrhea. It plays a role in warming the meridians and dispelling cold, promoting qi and blood circulation, removing blood stasis and relieving pain, providing a new direction for the external treatment strategy of traditional Chinese medicine for primary dysmenorrhea.