30 results about "Erigeron breviscapus" patented technology

The invention discloses a preparation method and a quality control method of a traditional Chinese medicine composition for stroke and middle meridian syndromes caused by extravasated blood blocking collaterals, and relates to the field of traditional Chinese medicines. The preparation method comprises the following steps: extracting salvia miltiorrhiza by adopting a composite enzymolysis-two-phase low-temperature extraction method, extracting erigeron breviscapus by adopting citric acid-ethanol extraction, extracting ligusticum wallichii by adopting double-template molecular imprinting-stir-frying with bran, extracting kudzu vine root by adopting a microwave method, adding auxiliary materials including hydroxypropyl methyl cellulose, sodium carboxymethyl cellulose and beta-cyclodextrin, drying by adopting a centrifugal spray dryer, and packaging by adopting a packaging machine. The Maillard reaction is regulated and controlled. The method has the advantages of efficient extraction, component optimization, preparation stabilization, drug effect enhancement, adverse reaction rate reduction, quality controllability and the like.

The present invention discloses a scutellarin extract and a preparation method thereof. By combining the aqueous solution obtained by removing ethanol from the water extract or ethanol extract of the dried whole herb of the plant of the genus Erigeron in the Compositae family with the water extract of the stem and / or leaf of Scutellaria baicalensis, a scutellarin extract can be obtained, where 60% ≤ the scutellarin content < 100 wt%. The plant of the genus Erigeron in the Compositae family is selected from any one or more of Erigeron breviscapus, Erigeron multiradiatus, Erigeron elongatus, Conyza canadensis, and Erigeron acer. The water extract of the stem and / or leaf of Scutellaria baicalensis can efficiently enzymatically convert scutellarin (baicalin) in the aqueous solution obtained by removing ethanol from the water extract or ethanol extract of the dried whole herb of the plant of the genus Erigeron in the Compositae family into scutellarin aglycone (scutellarin). The present invention uses the plant of the genus Erigeron in the Compositae family as the raw material for preparing scutellarin, and fully utilizes the stems and leaves of Scutellaria baicalensis, reducing the cost of producing scutellarin; the content of scutellarin in the product obtained by the preparation method is high, and the production process is simple and feasible.

The invention discloses a construction method of an agrobacterium tumefaciens-mediated erigeron breviscapus genetic transformation system, which comprises the following steps: putting an erigeron breviscapus explant into a prepared transgenic infection solution containing recombinant plasmid 1300-EbGRF4-GFP agrobacterium tumefaciens, and sequentially carrying out vacuum infiltration transformation treatment, then respectively and sequentially placing in an erigeron breviscapus co-culture medium, a recovery culture medium and a screening culture medium for culturing to obtain new proliferated calluses; and transferring the new proliferated callus into a bud initial culture medium to induce budlets, culturing in a bud differentiation culture medium, differentiating into resistant seedlings, and transferring into a rooting culture medium to obtain a transgenic plant. The method disclosed by the invention has the advantages that the method is used for inducing calluses of the erigeron breviscapus and efficiently transforming agrobacterium tumefaciens mediation, so that the erigeron breviscapus can quickly obtain target characters, the self genetic barrier of the erigeron breviscapus can be broken through, and a new variety which is difficult to produce through a traditional breeding mode can be obtained.

The present invention belongs to the field of traditional Chinese medicine pain-relieving patches, and specifically discloses a preparation method and application of a self-heating product for dredging collaterals and relieving pain; this method uses a variety of Chinese herbal medicines including Erigeron breviscapus as raw materials, and obtains an extract for dredging collaterals and relieving pain through extraction and concentration processes, and then dilutes it and soaks non-woven fabric to make a medicinal paper layer. At the same time, a heat-generating layer is made by wrapping heat-generating materials such as iron powder and activated carbon with a film-coated non-woven fabric, and is fixed to the medicinal paper layer and the anti-adhesive layer by hot pressing to obtain a self-heating product. In addition, non-steroidal anti-inflammatory drugs such as ibuprofen can also be added to the extract for dredging collaterals and relieving pain to enhance the pain-relieving effect. During the preparation process, various process parameters such as extraction temperature and concentration pressure need to be strictly controlled to ensure product quality. After the product is prepared, it needs to be packaged in a sealed bag to isolate air to maintain its self-heating effect and medicinal efficacy. The present invention has the effects of dredging collaterals and relieving pain, and warming and soothing, and is suitable for relieving pain and discomfort caused by rheumatism, arthritis, etc.

The invention relates to a KASP molecular marker associated with scutellarin of erigeron breviscapus and application of the KASP molecular marker, and belongs to the technical field of genetic engineering. The primers of the KASP molecular marker associated with the scutellarin of the erigeron breviscapus comprise a forward primer 1, a forward primer 2 and a common reverse primer; the nucleotide sequence of the forward primer 1 is as shown in SEQ ID NO. 1; the nucleotide sequence of the forward primer 2 is as shown in SEQ ID NO. 2; the nucleotide sequence of the common reverse primer is as shown in SEQ ID NO.3. By utilizing the KASP technology, the SNP site and the corresponding primer combination provided by the invention not only improve the plant variety selection efficiency, but also are beneficial to shortening the breeding cycle and accelerating the cultivation of high-quality plant varieties. The markers have good genetic stability and high resolution, are suitable for high-throughput detection, and have significant application value in the field of plant breeding.

The invention discloses a preparation method of a compound preparation applied to eye diseases, which comprises the following steps: S1, mixing cleaned, dried and crushed chrysanthemum powder, erigeron breviscapus powder and pseudo-ginseng root powder according to a weight ratio of 10: (3-8): (1-5) to obtain compound powder, sieving with a 60-100-mesh sieve, adding deionized water according to a weight ratio of 1: (10-20), soaking in a water bath oscillator, and filtering with a filter screen to obtain filtrate; and then slightly boiling and decocting. The ophthalmic compound preparation prepared by the invention comprises basic components including a free amino acid extract, vitamin A ester, glycosaminoglycan, carboxymethyl chitosan and aminoethanesulfonic acid which are included by a compound hydrophilic polymer, and a gel matrix component consisting of polyglutamic acid and polygonatum polysaccharide, when the compound preparation is used, a specific buffer solution or an isotonic solution does not need to be adopted for dissolution, meanwhile, the prepared compound preparation for the eyes can improve the low adhesiveness of traditional eye washing liquid components, especially small molecule components, and the defects that traditional eye washing liquid is large in size, inconvenient to carry and poor in solution storage stability are overcome.

The invention provides a traditional Chinese medicine compound for promoting blood circulation to remove blood stasis, promoting qi circulation and dredging collaterals and suitable for rehabilitation of qi stagnation and blood stasis type diseases, and belongs to the technical field of traditional Chinese medicine rehabilitation treatment. The formula is prepared from radix clematidis, radix cyathulae, astragalus membranaceus, cassia twig, garden balsam stem, vinegar frankincense, vinegar myrrh, safflower carthamus, angelica sinensis, radix paeoniae rubra, erigeron breviscapus, golden cypress and rheum officinale. All the medicines are combined to achieve the effects of promoting blood circulation to remove blood stasis and promoting qi circulation to dredge collaterals. The traditional Chinese medicine composition has a clinical curative effect on various qi-stagnation and blood stasis type diseases, can improve the sleep quality of patients, has no adverse reaction, and is easier to accept by the patients compared with oral medicines. The traditional Chinese medicine compound for promoting blood circulation to remove blood stasis, promoting qi circulation and dredging collaterals has the effects of promoting blood circulation to remove blood stasis, promoting qi circulation and dredging collaterals, has a good curative effect on qi stagnation and blood stasis type diseases after being used for a long time, and can be widely used for treating various diseases in the rehabilitation department.

The present invention discloses a mutant of Erigeron breviscapus with enhanced chalcone synthase activity and / or selectivity, and a screening method and application thereof. p The invention relates to a recombinant plasmid for synthesizing phloretin using ‑coumaric acid as a substrate. Through structure prediction and catalytic mechanism, sites related to the catalytic activity and selectivity of EbCHS are screened, and effective mutants and effective mutation sites are obtained through experimental screening. EbCHS is compared with different CHS sequences, and screening experiments are performed to obtain effective mutants of non-conserved amino acid sites adjacent to the above-mentioned effective mutation sites. The two obtained effective mutants are combined in pairs to obtain relevant detection information. Based on the above-mentioned experimental information, a database is built and machine learning modeling is predicted. After experimental verification, EbCHS with significantly enhanced activity and selectivity in the phloretin synthesis pathway is obtained, which can be used as p ‑coumaric acid as a substrate for efficient synthesis of phloretin and reduced by-products, providing technical support for promoting the industrial production of phloretin.

The invention provides a traditional Chinese medicine preparation for cerebral arterial thrombosis and a preparation method thereof, and relates to the technical field of traditional Chinese medicine pharmacy, the traditional Chinese medicine preparation is prepared from trichosanthes kirilowii maxim, inula flower, tamarix chinensis, arisaema cum bile, calculus bovis factitius, hawksbill, erigeron breviscapus, rhodiola rosea, radix paeoniae rubra, radix curcumae, herba lycopi, campsis grandiflora, catechu and benzoin. Comprehensive animal experiment and clinical research results show that the traditional Chinese medicine preparation has a remarkable treatment effect on cerebral arterial thrombosis, mNSS scores of MCAO rats can be remarkably reduced, the cerebral infarction volume can be reduced, meanwhile, inflammatory response balance in vivo can be adjusted, and inflammation-mediated brain injury can be relieved; the preparation can also significantly reduce the NIHSS score and the traditional Chinese medicine syndrome score of the cerebral arterial thrombosis patient, increase the BI score, effectively regulate the serum inflammatory factor balance, and significantly improve the total effective rate of clinical treatment. In conclusion, the preparation plays the roles of nerve protection, anti-inflammation and function recovery promotion through multiple targets, and a safe and effective new scheme is provided for treatment of cerebral arterial thrombosis.

The invention discloses a construction method and application of an erigeron breviscapus Cas9 gene editing system, and the construction method comprises the following steps: selecting an erigeron breviscapus EbPDS gene sequence and designing a target site, amplifying a target fragment by taking a PGTR plasmid as a template, and connecting the fragment to an expression vector PV58-Cas9 through homologous recombination to obtain the erigeron breviscapus Cas9 gene editing system. An erigeron breviscapus leaf explant is placed in a suspension infection solution containing PV58-Cas9-EbPDS agrobacterium to be infected, and then is respectively cultured in an erigeron breviscapus co-culture medium, a delayed screening culture medium and a screening culture medium to obtain a new callus with successful transgenosis. The method has the advantages that (1) a gene editing system constructed by the recombinant plasmid PV58-Cas9-EbPDS is efficient and feasible; (2) the EbPDS gene is successfully applied to CRISPR / cas9 mediated genome editing, and individuals with specific genotypes can be quickly screened out by observing whether offspring plants have a whitening phenotype phenomenon or not, so that an important means is provided for erigeron breviscapus gene function research and gene editing breeding.

The invention specifically discloses an optimized extraction and purification method of an erigeron breviscapus general flavone extract with an anti-oxidation effect, and belongs to the field of natural product extraction. The ultrasonic-assisted extraction technology is adopted, the response surface optimization method is combined, the total flavonoids are optimized and extracted, and the optimal extraction condition is obtained. The total flavonoids are purified by adopting a macroporous adsorption resin chromatography method, the macroporous adsorption resin is good in stability, strong in adsorption capacity, easy to elute and strong in regeneration capacity, the optimal purification condition is determined by exploring the influence of different factors such as temperature, time and pH on the adsorption process of the macroporous resin, and the antioxidant activity of the purified total flavonoids is further explored.

This invention provides a method for extracting active ingredients from *Erigeron breviscapus*, belonging to the field of pharmaceutical technology. Based on the differences in the physicochemical properties of the compounds, this invention first achieves preliminary separation of caffeic acid esters and scutellarin, followed by further purification and separation based on the properties of the two types of substances. Specifically, this invention uses ethanol-water solutions of different concentrations to extract caffeic acid esters stepwise, and uses macroporous and microporous resins for preliminary separation of the caffeic acid esters. Scutellarin is then preliminarily separated through acid-base treatment. Finally, high-purity scutellarin, 3,5-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, and scutellarin are obtained through reversed-phase industrial chromatography. This invention achieves the extraction and separation of multiple active ingredients from *Erigeron breviscapus*, and all solvents used in the extraction and separation process are recyclable, resulting in low cost.

The present invention relates to a preparation method of an Erigeron breviscapus extract, its product and application. The preparation method of the Erigeron breviscapus extract comprises the following steps: mixing an Erigeron breviscapus raw material with an extraction solvent and then carrying out water bath heating extraction, centrifuging and filtering the extraction solution to obtain a filtrate, thus obtaining the extract; the extraction solvent is selected from a deep eutectic solvent or a mixed solvent of a deep eutectic solvent and water. This preparation method creatively uses a deep eutectic solvent or a mixed solvent of a deep eutectic solvent and water to extract the Erigeron breviscapus raw material. On the one hand, it can make the contents of scutellarin and total flavonoids in the obtained extract high, providing an effective strategy for the utilization of the Erigeron breviscapus raw material and the preparation of scutellarin; on the other hand, the obtained extract also has excellent inhibitory activities against hyaluronidase, elastase and collagenase, excellent promoting expression activities of aquaporin, filaggrin and involucrin, excellent anti-free radical activity and excellent anti-aging activity.

The invention relates to the technical field of biological medicine, in particular to an anti-allergic composition, a preparation method thereof and application of the anti-allergic composition as a skin repairing agent, and the anti-allergic composition comprises a Sophora davurica DAVIDII seed extract, an ERIGERON BREVISCAPUS extract and a Dendrobium nobile (DENDROBIUM NOBILE) extract. Meanwhile, the invention further provides the anti-allergic composition, a preparation method of the anti-allergic composition and application of the anti-allergic composition as a skin repairing agent, and the anti-allergic composition is derived from whole plants and can achieve a good synergistic effect in the anti-allergic aspect.

The present invention provides a camellia oil anti-wrinkle, anti-aging, antioxidant cream and a preparation method thereof, belonging to the technical field of cosmetics. The preparation method comprises the following steps: adding glycerol, 1,3-butanediol, sorbitol, sodium hyaluronate, ethylhexylglycerin, methylparaben into water according to the formula, heating and stirring for 10-20 min, then successively adding camellia oil, plant fermentation active substances, squalane, L-arginine, tocopherol, PEG-7 glyceryl cocoate, cetearyl alcohol, glyceryl behenate and continuing to stir for 20-40 min, then homogenizing for 4-6 min, sterilizing, and discharging to obtain the product; wherein, the plant fermentation active substances are obtained by crushing, air explosion and fermentation treatment of Erigeron breviscapus and Plantago asiatica, and then through heating extraction and supercritical extraction; the fermentation bacteria are Bacillus subtilis and / or Lactiplantibacillus plantarum. The cream of the present invention has stable storage performance, is mild and moisturizing, can timely supplement moisture and nutrition to the skin, and has excellent antioxidant, anti-aging and anti-wrinkle effects.

The invention discloses a traditional Chinese medicine injection for treating cardiovascular and cerebrovascular diseases and a preparation method thereof, and relates to the technical field of traditional Chinese medicine injections.The injection is an erigeron breviscapus freeze-dried powder injection, takes an erigeron breviscapus extract as a unique active ingredient and contains pharmaceutical auxiliary materials; wherein each unit of the preparation contains 3-5g of erigeron breviscapus total flavonoids, the water content of the freeze-dried powder injection is less than or equal to 3.0%, and the pharmaceutical auxiliary materials comprise a stabilizer and a supporting agent; the stabilizer is one of sodium pyrosulfite or sodium hydrogen sulfite, and the addition amount of the stabilizer is 0.05-0.1% of the total weight of the preparation; the supporting agent is a combination of mannitol and glucose, and the addition amount of the supporting agent is 3-5% of the total weight of the preparation, according to the scheme, through purification-molding-quality control three-in-one innovation, on the premise that no high-end equipment is added, four breakthrough of safety, effectiveness, stability and economical efficiency is achieved, the upgrading requirement of the traditional Chinese medicine injection industry is completely met, and the application prospect is wide. The problem of safety of traditional Chinese medicine injections is effectively solved.

The present invention discloses a method for constructing and applying a Cas9 gene editing system for Erigeron breviscapus, comprising the following steps: selecting an Erigeron breviscapus EbPDS gene sequence and designing a target site, amplifying a target fragment using a PGTR plasmid as a template, connecting the fragment to an expression vector PV58-Cas9 by homologous recombination, infecting an Erigeron breviscapus leaf explant in a suspension infection solution containing PV58-Cas9-EbPDS Agrobacterium, and then culturing the explant in a Erigeron breviscapus co-culture medium, a delayed screening medium, and a screening medium, respectively, to obtain newly generated callus tissue. The advantages of the present invention are: (1) the gene editing system constructed with the recombinant plasmid PV58-Cas9-EbPDS is efficient and feasible; (2) the EbPDS gene is successfully applied to CRISPR / Cas9-mediated genome editing, and individuals with a specific genotype can be quickly screened out by observing whether the offspring plants show an albinism phenotype, thereby providing an important means for gene function research and gene editing breeding in Erigeron breviscapus.

This invention belongs to the field of drug detection technology, specifically relating to a detection method for *Erigeron breviscapus* (Dendrobium nobile) formula granules. These granules are made from *Erigeron breviscapus* medicinal slices through decoction, concentration, drying, and granulation. The detection method includes morphology, identification, inspection, extractives, characteristic chromatograms, and content determination. The identification method uses thin-layer chromatography; the characteristic chromatogram uses high-performance liquid chromatography, with the baicalin reference peak as the S peak, indicating 11 characteristic peaks; and the content determination measures the content of baicalin. The detection method established by this invention improves the detection requirements for *Erigeron breviscapus* medicinal materials, medicinal slices, standard decoctions, and formula granules, contributing to the establishment of a comprehensive quality control system for various *Erigeron breviscapus* products and helping to ensure the quality and clinical efficacy of *Erigeron breviscapus* formula granules.

The utility model relates to the field of erigeron breviscapus seed screening, and discloses a seed screening device for erigeron breviscapus planting, which comprises a base, the top of the base is fixedly connected with a collecting shell, a sliding assembly is arranged in the collecting shell, the sliding assembly comprises two symmetrically distributed sliding grooves formed in the two sides of the collecting shell, and the sliding grooves are communicated with the collecting shell. Four sliding grooves are formed in the bottom of the collecting shell, sliding blocks are slidably connected into the four sliding grooves, a screening assembly is arranged in the collecting shell, a motor is fixedly installed at the top of the base, and the output end of the motor is fixedly connected with a first rotating shaft. Through reciprocating motion of the first screening shell and the second screening shell, seeds can be accelerated to pass through the first screening shell and the second screening shell at the moment, the screening time is shortened, more unnecessary workload of workers is reduced, and use of the workers is greatly facilitated.

The invention discloses an asexual propagation method for erigeron breviscapus, and belongs to the technical field of plant asexual culture. According to the method, erigeron breviscapus varieties with excellent characters are subjected to plant division treatment, small erigeron breviscapus plants obtained through plant division are put into a diluted potassium permanganate solution with the mass concentration of 95% to be soaked and properly turned over, and water culture is conducted after surface moisture is aired. According to the method disclosed by the invention, the separated plantlets can be subjected to water culture, the seedling recovery period is short, the growth vigor is strong, the tillering is fast, the plantlets are not easy to bloom early, the effective content is high, the yield is also high, the keep-alive rate is from 10% to more than 95%, and excellent characters of a female parent are also maintained, so that progeny plants and the female plant have the same quality and characteristics, heritable variation is reduced, and the survival rate is increased. And moreover, the method is simple and convenient to operate, relatively low in cost, relatively good in economic benefit and suitable for large-scale production.

The utility model relates to the field of Chinese herbal medicine, and discloses a screening device for processing erigeron breviscapus, which comprises a collecting round box, side plates fixedly mounted at the top of the collecting round box, a driving motor fixedly mounted on the inner wall of the bottom of the collecting round box, connecting plates fixedly connected to the side plates, and a mounting frame arranged between the two groups of side plates, when the vibration motor drives the mounting frame to shake, particles on erigeron breviscapus are removed, and the purity of medicines is ensured through separation treatment, so that unnecessary side effects can be avoided, and the effectiveness and safety of the medicines are ensured; the particles are screened out of the screening holes in the rotating process of the screening rod, the particles can be ensured to be consistent in size and weight through particle screening, and accurate metering and taking are facilitated. The method is especially important for medicine treatment requiring strict dosage control, and a more reliable treatment effect can be provided.

The invention relates to the field of traditional Chinese medicines, and particularly discloses a traditional Chinese medicine composition, a pharmaceutical preparation and a preparation method and application thereof. The traditional Chinese medicine composition comprises the following components in parts by weight: 6-12 parts of eclipta, 3-9 parts of pseudo-ginseng, 6-12 parts of dogwood, 9-15 parts of teasel root, 6-12 parts of grifola, 3-6 parts of felwort, 3-10 parts of polygala tenuifolia, 2-3 parts of hypericum perforatum, 10-30 parts of sedum sarmentosum, 3-9 parts of inula flower, 6-9 parts of curcuma zedoary, 5-10 parts of cowherb seed, 6-12 parts of changium smyrnioides and 9-15 parts of erigeron breviscapus. The traditional Chinese medicine composition can regulate and control non-alcoholic steatohepatitis related signal channels on HepG2, LX2, THP1 and HUVECT1 cell lines, reduce the fat content in a HepG2 cell high-fat model, reduce the serum alanine aminotransferase, serum aspartate aminotransferase, serum low-density lipoprotein cholesterol and liver triglyceride level of a mouse non-alcoholic steatohepatitis model, and improve the non-alcoholic steatohepatitis content in the mouse non-alcoholic steatohepatitis model. The traditional Chinese medicine composition is capable of reducing liver fat accumulation, relieving liver injury and improving liver fibrosis pathology, and is used for related treatment or adjuvant treatment of non-alcoholic steatohepatitis.

The invention discloses a traditional Chinese medicine formula for preventing and treating cerebrovascular diseases. The traditional Chinese medicine formula comprises Yulangsan, bauhinia championii, corydalis amabilis, lumbricus, astragalus membranaceus, ligusticum chuanxiong hort, cassia twig, radix paeoniae rubra, safflower, rhizoma acori graminei and erigeron breviscapus. The traditional Chinese medicine composition has good clinical effects on treating cerebral atherosclerosis, stenosis, infarction, atherosclerosis and apoplexy sequelae, hemiplegia, aphasia and the like caused by cerebral atherosclerosis, stenosis, infarction and atherosclerosis, and can gradually or basically recover the apoplexy sequelae caused by cerebral atherosclerosis, stenosis, infarction and atherosclerosis; yulangsan and bauhinia championii have the effects of relaxing tendons and activating collaterals, and corydalis amabilis, ligusticum wallichii, radix paeoniae rubra and safflower have the effects of promoting blood circulation and removing blood stasis and are The earthworm, the cassia twig, the rhizoma acori graminei and the erigeron breviscapus are used for dredging brain collaterals and are assistant and guide; the whole formula has the effects of tonifying qi, activating blood, dispersing blood stasis, dredging collaterals, inducing resuscitation and inducing resuscitation.

Provided is a traditional Chinese medicine composition for reducing blood pressure, which is prepared from the following active pharmaceutical ingredients in parts by weight: 20-35 parts of Uncaria rhynchophylla, 15-30 parts of Laggera pterodonta, 10- 30 parts of Rubus sachalinensis, 15-30 parts of Chinese yam, 10-15 parts of Erigeron breviscapus, and 5-15 parts of Tetrapanax papyrifer. The preparation method comprises: crushing part of the active pharmaceutical ingredients into medicinal powder, preparing medicinal juice from the remaining active pharmaceutical ingredients, uniformly mixing the pharmaceutical juice with the pharmaceutical powder, forming the mixture into strips, kneading into pills, and air-drying. Also provided is use of the traditional Chinese medicine composition in preparing a drug for preventing and treating hypertension diseases.

The invention discloses an erigeron breviscapus chalcone synthase activity and / or selectivity enhanced mutant as well as a screening method and application thereof. The construction method comprises the following steps: constructing a recombinant plasmid for synthesizing phloretin by taking p-coumaric acid as a substrate, screening out sites related to the catalytic activity and selectivity of EbCHS through structural prediction and a catalytic mechanism, and carrying out experimental screening to obtain effective mutants and effective mutation sites; comparing the EbCHS with different CHS sequences, and carrying out screening experiments to obtain effective mutants of non-conservative amino acid sites adjacent to the effective mutation sites; combining the two obtained effective mutants in pairs to obtain related detection information; according to the experimental information library building, machine learning modeling prediction and experimental verification, the EbCHS with obviously enhanced activity and selectivity in the phloretin synthesis route is obtained, the phloretin can be efficiently synthesized by taking p-coumaric acid as a substrate, byproducts are reduced, and a technical support is provided for promoting the industrial production of the phloretin.

The invention discloses a genetic transformation method of erigeron breviscapus chloroplast. According to the method, plasmids containing target genes are introduced into explants prepared from erigeron breviscapus leaves through a gene gun method, and erigeron breviscapus transfection plants are obtained; wherein the parameters of the gene gun bombardment are as follows: the plasmid DNA concentration is 1-1.5 [mu] g / [mu] l, the number of bombardment times is 1-2, the target distance is 3-9 cm, the bombardment pressure is 900-1100 psi, and the vacuum degree is 26-28 mmHg. By adopting the method established by the invention, the homogenized erigeron breviscapus transfection plant can be efficiently obtained. The method has an important application value.

The invention relates to an application of Erigeron breviscapus transcription factor EbbHLH80 in enhancing the salt tolerance of tobacco and a method for enhancing the salt tolerance of tobacco plants, belonging to the technical field of genetic engineering.

This invention provides a composition with menstrual regulating and blood-activating effects and its application in the preparation of external products, relating to the field of biomedical technology. This invention utilizes a composition composed of Chinese herbal raw materials including *Cephalotaxus fortunei* bark, star anise, *Erigeron breviscapus*, *Piper longum*, *Caulis thunbergii*, *Radix Achyranthis Bidentatae*, *Radix Scutellariae*, *Radix Achyranthis Bidentatae*, *Radix Panax notoginseng*, *Radix Bambusae in Taeniam*, and *Radix Achyranthis Bidentatae* to prepare a traditional Chinese medicine acupoint patch for treating or improving primary dysmenorrhea. The herbal acupoint patch can inhibit the expression of inflammatory factors, downregulate the expression level of COX-2, thereby inhibiting PGF2α, increasing the level of PGE2, and reducing uterine tissue inflammation, achieving multi-target treatment of primary dysmenorrhea. The herbal acupoint patch utilizes the power of the medicine through the acupoint, and the acupoint utilizes the properties of the medicine. The meridians are the channels, and the acupoints are the gateways. The medicinal power directly reaches the Chong and Ren meridians and the uterus, so that cold is warmed and dispelled, blood stasis is cleared and eliminated, and qi and blood flow smoothly, thus curing dysmenorrhea. It plays a role in warming the meridians and dispelling cold, promoting qi and blood circulation, removing blood stasis and relieving pain, providing a new direction for the external treatment strategy of traditional Chinese medicine for primary dysmenorrhea.

The utility model relates to the field of Chinese herbal medicine, and discloses an erigeron breviscapus Chinese herbal medicine seedling transplanting device which comprises a square frame, a vertical main rod is fixedly arranged at the position, corresponding to the middle, of the top of the square frame, two sets of side plates are connected to the square frame in a sliding mode, and a first arc plate is fixedly connected to the bottom of one set of side plates. A first arc plate is fixedly arranged at the bottom of one group of side plates, a second arc plate is fixedly arranged at the bottom of the other group of side plates, a soil transplanting assembly is further included, the first arc plate and the second arc plate are close to each other to extrude soil and erigeron breviscapus, the root system of the erigeron breviscapus can be protected against interference through soil transplanting, and the soil structure around the root system keeps stable; in addition, due to the fact that the erigeron breviscapus does not need to adapt to brand-new soil conditions, the transplanting method can reduce the pressure generated by the fact that the plants adapt to the new environment, and the withering risk is reduced.

The invention provides a metal organic framework which is prepared from zinc nitrate and ligands as raw materials, and the ligands are terephthalic acid (PTA) and phenylenediboronic acid (BDBA); the molar ratio of zinc to the ligand (PTA + BDBA) is (1: 4)-(5: 4), and the molar percentage of BDBA in the ligand is 0-50%. The invention also provides a preparation method of the metal organic framework and a purification method of scutellarin. According to the method, firstly, the scutellarin in the traditional Chinese medicinal material water extract is efficiently adsorbed through the boric acid group-MOF, then the MOF material is eluted by adopting DES as an eluent, the volume of the eluent is small, the content of the scutellarin is high, the amount of water added during water precipitation is small, and the content of the scutellarin is high. The practical application of the preparation of the scutellarin in the traditional Chinese medicine erigeron breviscapus is facilitated.