49 results about "Screening duration" patented technology

The invention relates to the technical field of screening of natural medicines and provides a high-content rapid screening method for magnetic beads of ocean natural active materials. The method is a screening and evaluating method for active materials based on magnetic bead bonds and high-content multi-target protein integration. According to the high-content rapid screening method, the configuration is as follows: a hardware system is composed of a permanent magnet, the magnetic beads and targets; a software system is responsible for acquiring hygroplasm data. Compared with a conventional ultraviolet screening method, the screening method has the advantages that the screening efficiency is obviously improved and the screening time is shortened; various biological activities can be investigated at the same time and the synergistic effect among compounds is found. The screening method is rapid, accurate, great in information amount and good in repeatability and can be used for screening a natural product library or a combinatorial chemistry library in a large scale.

The invention discloses a method for screening protein aptamers by using a micro-fluidic chip. The method comprises steps that: a preset initial nucleic acid library is first incubated with a non-target protein, such that an optimized nucleic acid library is obtained; a target protein is incubated with microspheres, the target-protein-modified microspheres are positioned in a channel of a micro-fluidic chip; the optimized nucleic acid library is delivered into the channel of the micro-fluidic chip; the materials are processed through washing and eluting, such that an initial screened nucleic acid library is obtained; PCR amplification is carried out upon the initial screened nucleic acid library, the amplification products are separated through an affinity column, and the products are processed from denaturative melting and desalting, such that a primary nucleic acid library is obtained; the primary nucleic acid library is used for replacing the initial nucleic acid library, and the previous steps are repeated until a nucleic acid library containing target aptamers is obtained. The method provided by the invention has advantages of good versatility, short screening time, high screening efficiency, and good screening effect.

The invention discloses a chromosome division phase image quality evaluation method based on deep learning, and belongs to the technical field of chromosome image processing. Through the deep learningmodel, the quality of the chromosome split-phase images can be scored, the image quality of the chromosome images can be accurately evaluated, the robustness for evaluating different types of chromosome split-phase images is high, and the operation efficiency of doctors is improved. According to the invention, the chromosome division phase image quality can be evaluated accurately and efficiently; the method can effectively improve the evaluation and selection efficiency of the chromosome division phase images, shortens the screening time of the chromosome division phase images, can effectively reduce the workload of doctors, is not interfered by the outside, is simple and reasonable in process, can be popularized and applied to the outside in a large scale, and is simple in deployment.

The embodiment of the invention discloses a question processing method and device based on semantic matching, a terminal and a storage medium, and the method comprises the steps of obtaining a to-be-analyzed question, processing the to-be-analyzed question, obtaining a label of the to-be-analyzed question, obtaining a standard question set corresponding to the label, determining semantic similarity between the to-be-analyzed questions and each standard question in the standard question set, screening out target standard questions from the standard question set based on the semantic similarity, determining answers corresponding to the to-be-analyzed questions based on target answers corresponding to the target standard questions, and displaying the answers corresponding to the to-be-analyzed questions. By implementing the method, the labels of the questions can be analyzed, and the answers to the questions are found based on the labels of the questions, so that the screening time of the answers to the questions is shortened, and the processing efficiency and processing accuracy of the questions are improved. The scheme is applied to a medical scene, for example, questions raised by a patient are intelligently answered, and digital medical treatment is realized.

The invention provides a method for screening a compound with lipase inhibition activity by magnetic bead separation. The method is used for rapidly screening the compound with lipase inhibition activity rapidly from fructus aurantii by adopting a magnetic bead separation and liquid chromatography-mass spectrometry combination method. Compared with the traditional method and the prior art, the method disclosed by the invention has the advantages of being capable of rapidly screening and identifying the compound with potential lipase inhibition activity from a mixture, further separating and preparing the target compound and carrying out activity verification, thereby greatly reducing the blindness of separating effective ingredients and improving the activity screening efficiency. During screening, the compound does not need to be prepared and separated, therefore the screening efficiency is remarkably improved and the screening time is shortened. In addition, the screening method has the characteristics of high speed, accuracy, good reproducibility and the like and can be popularized to be used for rapidly screening the compound with lipase inhibition activity from natural medicines or Chinese medicine mixture. The compound with lipase inhibition activity, obtained by using the method disclosed by the invention, can be applied to preparation of diet pills and lipid-lowering medicaments.

The embodiment of the invention discloses a screen locking method and device, a terminal and a memory medium. The method comprises the steps of obtaining a target face image of a current user when a screen is in a bright screen state; matching the target face image with a pre-stored child face image, and determining a target identity of the current user; obtaining current distance between the current user and the screen if the target identity is a child identity, and recording current bright screen duration; and carrying out black screen locking on the screen if it is detected that the currentdistance or the current bright screen duration satisfies a screen locking condition. According to the technical scheme provided by the embodiment of the invention, watching distance and watching duration of a child can be automatically controlled, so physical and psychological health of the child is prevented from being influenced due to long-time near distance watching is avoided.

The invention relates to a multimedia play method for switching from a first channel to a second channel and a multimedia play device. The multimedia play method for switching from the first channel to the second channel comprises the steps that when a player receives a complete frame of a multimedia code stream of the second channel, the player sends a stop command to a decoder, and the decoder stops transmitting a video code stream in a multimedia code stream of the first channel to the player. With the adoption of the multimedia play method and the multimedia play device, the blank screen duration time during channel switching can be shortened significantly, so that a watching feel of a video watcher can be raised.

The invention discloses an evidence-based method for screening gastric cancer prognosis molecular markers. The method comprises the steps as follows: 1), an inclusion criterion is set for a gastric cancer prognosis biomarker experimental research; 2), a research evidence retrieval strategy is set; 3), the prognosis biomarkers are screened preliminarily; 4), research data are extracted; 5), quality assessment on the experimental research quality is performed; 6), standardized data are processed; 7), the prognosis biomarkers are screened; and 8), according to the screening criterion, the gastric cancer prognosis biomarkers having potential application values are screened, and the prognosis values are described. In conclusion, the evidence-based method for screening the gastric cancer prognosis biomarkers is provided, and the gastric cancer prognosis biomarkers are screened, so that the screening cost is reduced, the screening time is shortened, professional thresholds are reduced, and the application values of the prognosis biomarkers are increased.

The invention relates to the technical field of video live broadcast and discloses a method and a system for quickly displaying a real-time video stream in an industrial scene. The method comprises the following steps of caching I frames / and P frames belonging to GOP picture frames on a buffer server side; receiving a code stream request message from a display terminal; according to the type of the current real-time picture frame in the MPEG video stream, obtaining the MPEG video stream; after a first frame is transmitted to a display terminal, inserting a sub-frame containing a cached I frame / and a cached P frame selectively. Therefore, the method can be based on the coding characteristics of the GOP picture frame; it is ensured that the display terminal can decode and acquire the video image corresponding to the first frame after receiving the first frame; and the video image is directly displayed without waiting for audio synchronization, so that the picture can be opened at the first time after successful decoding, the black screen duration is effectively shortened, the user experience is improved, and the method is particularly suitable for real-time monitoring application ofequipment in an industrial scene.

The invention discloses a screening device used for a finished nut product, and relates to the technical field of machining. The screening device comprises a base, wherein two symmetric longitudinal damping parts are fixedly connected to one surface of the base; one ends of the two longitudinal damping parts are fixedly connected with a first supporting seat and a second supporting seat respectively; vibration motors are fixedly connected with the top surface of the first supporting seat and the bottom surface of the second supporting seat; two transverse guide rods are fixedly connected between inner surfaces of the first supporting seat and between inner surfaces of the second supporting seat respectively; and a screening mechanism is connected to peripheral side surfaces of the two transverse guide plates. By means of design of the screening mechanism, a multi-stage separation process of the nut can be completed automatically, and besides, by configuration positions of multiple sieve pore parts in a screening area, pore diameter and density design and speed-limiting design of speed-limiting baffle plates, on one hand, the purpose of screening step by step can be achieved, and onthe other hand, the screening time of a nut material in the screening area can be effectively prolonged.

The invention discloses a method for screening melanin formation resistant medicines on the basis of magnetic bead separation. Target protein conjugates are analyzed by the aid of magnetic separation and chemical information acquisition (liquid chromatography-mass spectrometry) technologies, and the method can be used for screening and evaluating melanin formation resisting effects of the medicines. The optimal incubation temperatures, the optimal incubation time, the optimal pH (potential of hydrogen) values of solution during incubation, the optimal ion strength and the optimal denaturation cleaning solution are screened by the aid of large quantities of experiments. Compared with the traditional methods for screening melanin formation resistant medicines, the method has the advantages that the screening efficiency can be obviously improved, and the screening time can be shortened; synergistic effects among compounds can be discovered, the method can be used for screening large-scale natural product libraries or combinatory chemical libraries, is suitable for screening the melanin formation resistant medicines (including chemically synthetic medicines and natural products) and discovering lead compounds at early stages, is speedy and accurate and is good in repeatability, and the like.

The invention discloses a screening method of a fenpropathrin pesticide degradation bacterium, which comprises the following steps: acquiring a sample; carrying out enrichment culture; selecting a culture medium, and culturing; separating and purifying; and obtaining the degradation bacterial strain, and preserving. The method overcomes the defects of time consuming, high workload and high cost in the traditional fenpropathrin pesticide degradation bacterium, is simple and convenient, shortens the screening time, and reduces the workload for screening.

The invention discloses a novel constructional engineering sand screening device which comprises a screening box body. A feeding hopper is installed on one side of the top of the screening box body; agroup of opposite fixed sliding rails are installed in the screening box body; connecting sliding rods are arranged on the fixed sliding rails; a screening frame is installed at the bottoms of the connecting sliding rods. a fixing plate is installed in the screen box body and is located on one side of the screening frame; a connecting rod is installed on one side of the screening frame and is slidably connected with the fixing plate; a telescopic spring sleeves the connecting rod; and a screened sand discharging mechanism is arranged on the screen box body. The novel constructional engineering sand screening device provided by the invention has the beneficial effects that the telescopic end of the hydraulic oil cylinder drives the screening frame to swing left and right to screen sand, sothat the screening speed is high, the working efficiency is improved, and the screening time is shortened; and an electric telescopic rod pushes a containing frame to move, so that automatic discharging can be achieved, manual taking-out is not needed, the workload is reduced, and time and labor are saved.

The invention discloses multi-grade separation ore sand screening equipment for building materials. The equipment comprises a support mounted on the ground, a first shell arranged on the support, a material guide plate arranged on a first housing and a screening device arranged in the first shell. The screening device comprises a second shell arranged in the first shell, multiple connecting blocksconnected to the first shell and the second shell, a feeding opening arranged below the second shell, a stepped screening structure arranged in the second shell, a transmission structure used for enabling the stepped screening structure to cyclically move and a discharging structure arranged below the stepped screening structure. Through the arrangement of the stepped screening structure, the occupied area of the ore sand screening equipment is effectively reduced, the sand stone screening duration is prolonged, the screening effect is enhanced, finally-obtained screening products are more standard, and the percent of pass is higher.

The invention relates to the technical field of early blight prevention material screening methods, and discloses a screening method for virus-free potato test-tube plantlet early blight prevention. The method includes the following steps that firstly, pathogenic bacteria are separated; secondly, the pathogenic bacteria are purified; thirdly, sporangium turbid liquid is prepared; fourthly, inoculation is carried out. Compared with a traditional screening method, the screening method for virus-free potato test-tube plantlet early blight prevention is small in error, short in screening time and low in screening cost. Meanwhile, the screening method for virus-free potato test-tube plantlet early blight prevention makes up for the blanks in the aspect of potato early blight research and prevention in the Xinjiang region. The screening method suitable for potato early-blight-prevention test-tube plantlets in the Xinjiang region is established, timeliness, effectiveness and accuracy of early blight identification are greatly improved, and the method plays has great significance in quick epidemic disease identification and prevention.

The invention relates to medicine, specifically, to methods for drug preparations screening, more specifically, to choosing a drug and its optimal dose to treat a particular patient.It is suggested to choose a drug preparation basing on the results of culturing of whole heparinized patient's blood in the presence of aqueous solutions of drugs to be compared, to analyze the ratio of -SH and -SS groups in the cellular fraction of patient's blood after culturing, and to choose the drug that makes the greatest value of the given ratio.The method allows decreasing the screening time down to a few hours and is useful in treatment of viral, cancerous, autoimmune, and other diseases.

The invention discloses a plant transgenic visual tracking expression system and application thereof. The plant transgenic visual tracking expression system is a plant expression vector which contains a visual marker gene expression kit, the visual marker gene expression kit consists of an expression kit of a transcription factor bi gene in the anthocyanin biosynthetic pathway and an expression kit of a transcription factor cl gene in the anthocyanin biosynthetic pathway. The color regulator gene is applied on plant genetic transformation operation, the screening work is effectively relieved after the transformation, according to the gene performance on the plant, the valuable transformation materials can be selectively kept, accordingly, the screening time and the research cost are greatly saved, the accuracy and the reliability of the experimental result are greatly improved, so good foundation is laid for carrying out analysis work after the transformation. In addition, the transient expression of the color gene is utilized, the transformation effect can be visually and more directly analyzed, and accordingly, various transformation influencing factors are prevented from being generated.

The invention discloses a novel polypeptide and application thereof in diagnosis and treatment of prostatic cancer, an extracellular recognition region sequence of a TABP-EIC cell is screened through a phage-polypeptide library, a polypeptide ligand obtained through screening can replace a traditional single-chain antibody to be used for preparing the TABP-EIC cell, and compared with a traditional method for preparing the TABP-EIC cell, the screening time of the sequence of the extracellular recognition region of the TABP-EIC cell is remarkably shortened, the binding efficiency of the screened polypeptide ligand and the related tumor specific antigen is higher, the recognition polypeptide ligand aiming at an antigen-indefinite tumor can be screened out, a new thought is provided for immunotherapy of the tumor, the screened polypeptide ligand has extremely high application value in tumor cell therapy.

The invention discloses an apparatus and a method for screening cell characteristic by a bioreactor, the cell characteristic screening method is characterized in that according to different metabolism rate of the cells under different cell states, by adding physical, chemical and biological factors in a cell characteristic screening process to cause the different specific growth rate of the cell, the cell with fast specific growth rate is the screened cell. The apparatus and method can realize the automated management during the screening process and a culture process, and enable continuous characteristic screening to pair cell (such as G418pressurization or continuous serum reduction), a lot of target protein or target cell can be simultaneously obtained while screening, in addition, the screening process of a reactor is capable of greatly shortening the screening time, reducing the workload and reducing the pollution rate, artificial factor can be better removed, and the apparatus can provide the guaranteed cell strain for industrial application.

The invention discloses universal length grading screening equipment and relates to the technical field of screening equipment. The equipment comprises a base; a discharging structure fixedly arranged on the upper wall of the left end of the base and located on the center line; a sorting structure fixedly arranged on the upper wall of the base and located on the right side of the discharging structure; and a conveying assembly movably arranged on the sorting structure. A plurality of workpieces can enter the sorting structure in sequence by means of transmission through the discharging structure; through cooperation of the sorting structure and the conveying assembly, at most four kinds of workpieces with different lengths can be rapidly conveyed and screened; and at most four kinds of workpieces with different lengths can be screened at the same time through overall cooperation of the equipment, the splitting length can be adjusted according to needs, the overall feeding and guiding screening speed is high, and screening time is shortened.

The invention relates to a coal screening equipment, which comprises a bottom plate and four circular vibrating screens distributed around the center of the bottom plate, screens with different apertures are distributed in the vibrating screen, and a plurality of screens installed on the bottom plate to store multiple screens. There is a storage rack, and a conveying device for transporting the screen to the corresponding vibrating screen is distributed above the bottom plate, and a lifting mechanism is provided on the bottom plate to drive the storage rack up and down. The invention realizes multi-aperture screening of raw coal without multiple screening, reduces screening steps, shortens screening time, and transports sieve plates with different apertures into the corresponding vibrating screen through the cooperation of the conveying device and the lifting mechanism, without manual installation , Reduce the work intensity of workers, shorten the screening preparation time, and enhance the screening efficiency.

The present invention involves a method of screening nail protein specific nucleic acid ligands using capillary electrical technology, including the following steps: design and build random 寡 nucleotide libraries, combined with capillary electrophoresis technology and SELEX screening method, which is rich and can be available to be ableThe oligonucleotide combined with nail protein, after cloning, sequencing, capillary electrophoretic analysis, and immunofluorescence verification, oligraphy nucleotides that can be combined with taiffendin, that is, the adaptation body.The present invention provides an efficient and special adaptive screening method.The advantage of the present invention is that the advantages of the capacity of the capillary tube are shortened, and the screening time can be shortened, and only 4 rounds of cycle can be completed. At the same time, the non -specific screening caused by the influence is reduced.

The invention relates to coal screening equipment which comprises a bottom plate. Four circular vibrating screens are distributed around the center of the bottom plate, and screen nets with differentapertures are distributed in the vibrating screens. The coal screening equipment further comprises a storage frame installed on the bottom plate for storing the multiple screen nets. A conveying device for conveying the screen nets into the corresponding vibrating screens is distributed above the bottom plate, and the bottom plate is provided with a lifting mechanism which drives the storage frameto ascend and descend. Multi-aperture screening of raw coal is realized, repeated screening is not needed, screening steps are reduced, screening time is shortened, screen plates with different apertures are conveyed into corresponding vibrating screens through cooperation of the conveying device and the lifting mechanism, manual installation is not needed, the work intensity of workers is reduced, screening preparation time is shortened, and screening efficiency is enhanced.

The invention relates to the field of bacterial colony screening, in particular to a bacterium screening reagent, a screening method, a kit and application. The invention provides a bacteria dissolving reagent, which is prepared from the following components: 10 to 30 mM of Tris-HCl, 10 to 20 mM of EDTA (Ethylene Diamine Tetraacetic Acid) and 0.01 to 0.55 mg / mL of lysozyme. According to the invention, the CRISPR Cas12a technology is applied to bacterial colony screening for the first time, and is improved and optimized based on the technical principle, so that the CRISPR Cas12a technology is more suitable for high-throughput screening. In plasmid design, a PAM sequence is introduced to the tail end of a gene, so that all crRNA sequences can simultaneously recognize a gene segment and a vector segment. In addition, automatic equipment and a large-batch screening reaction system and reaction conditions are utilized, so that the method is fully suitable for an automatic implementation scheme of bacterial colony screening, the screening time is greatly shortened, and the strain breeding speed and the strain screening efficiency are greatly improved.

The invention discloses automatic screening equipment based on coal, relates to the technical field of automatic screening, and provides the following scheme for solving the problems that when the coal is automatically screened, due to the fact that accurate screening cannot be achieved manually, the quality of the coal is prone to being mixed, the percent of pass is reduced, the manual salary ishigh, the screening time is long, the capital is wasted, and the efficiency is affected. The automatic screening equipment is characterized in that a motor is fixedly arranged at the bottom of an outer box in a sleeved mode; an output shaft of the motor is fixedly connected to a first bevel gear; the right side of the first bevel gear is provided with a vibration mechanism; the left side of the first gear is provided with a push-pull mechanism; the right side of the vibration mechanism is provided with a transmission mechanism; the vibration mechanism comprises a first fixing rod fixedly connected to the top of the outer box; and the top of the first fixing rod is fixedly connected to a first bearing. The automatic screening equipment is automatic in operation, not only accurately screensexcellent coal according to standards and improves the percent of pass, but also saves labor funds, shortens the screening time and improves the efficiency.

The invention relates to the technical field of genetic breeding, in particular to an InDel marker remarkably related to soybean cell nucleus male sterility, application of the InDel marker and a method for identifying soybean plant fertility by using the InDel marker. The InDel marker is located between 22776268bp and 222815034bp of the No.13 chromosome of the soybean, the InDel marker can be used for rapidly identifying the nuclear male sterile plant of the soybean plant, meanwhile, the screening time is shortened, and the screening efficiency and the screening accuracy are improved.