3 results about "Screening duration" patented technology

The application provides a kinase inhibitor leading compound, a virtual screening method thereof and application of the kinase inhibitor leading compound in preparation of branched-chain alpha-keto acid dehydrogenase kinase inhibitors and / or drugs for treating diseases mediated by branched-chain alpha-keto acid dehydrogenase kinase. The virtual screening method provided by the application combines various virtual screening platforms to screen a large-scale compound library, improves the true positive rate of screening results, reduces the number of compounds needing experimental screening, and saves screening time and cost. In addition, the compounds I-III screened out with good binding affinity to BCKDK can be used in researches on targeting BCKDK to treat major diseases such as obesity, insulin resistance, maple syrup urine disease, neurological dysfunction, liver cancer, colorectal cancer and tumor cachexia.

This invention provides a method for screening highly specific nucleic acid aptamers and specific *Lactobacillus helveticus* aptamers, belonging to the field of nucleic acid aptamers. Specifically, the method for screening highly specific nucleic acid aptamers provided by this invention includes the following steps: S1: A second library is obtained after N rounds of forward screening of a first library; S2: A third library is obtained after M rounds of reverse screening of the second library; S3: The third library is obtained after X rounds of forward screening of the third library to obtain selected random single-stranded oligonucleotides, which are then subjected to PCR amplification, purification, cloning, and sequencing; S4: The sequences cloned and sequenced in step S3 are used for affinity and specificity assays to select sequences with high affinity and specificity, thereby obtaining highly specific nucleic acid aptamers. The aptamers provided by this invention have the advantages of high specificity and strong affinity, and the construction method of the aptamers provided by this invention has the advantage of short screening time.

This utility model relates to the field of raw material screening technology, specifically to a raw material grading, screening, and conveying device. The device includes a screening mechanism comprising a housing with an inlet at the top and a first outlet on one side. A first support member is mounted on the inner wall of the housing. A first spring is mounted on top of the first support member. A connecting member is mounted on top of the first spring. A screen is hinged to one end of the connecting member. A vibrating motor is mounted at the bottom of the screen. A first drive mechanism for rotating the screen is mounted below the screen. A second support member is slidably mounted on the rear wall of the housing. A second spring is mounted on top of the second support member. A third support member is mounted on top of the second spring. A first electric cylinder is mounted on one side of the second support member. A fourth support member is mounted at the first outlet. This device can perform screening in a horizontal state, extending the screening time and ensuring more thorough screening of the raw materials.