198 results about "Keto acid" patented technology

The present technical solution discloses a method for producing fermented fish sausages and fermented fish sausages that improve the distinctive flavor of the sausage. The present invention adds 5-pyridoxal phosphate, which is a cofactor that catalyzes the transaminase reaction and can catalyze the leucine in the fish protein to produce α-ketoisocaproic acid, thereby increasing the content of 3-methylbutyraldehyde; by adding a composite fermentation agent, Lactococcus lactis subspecies cremoris contains transaminase, which promotes the catabolism of leucine and isoleucine to produce 3-methylbutyraldehyde and 2-methylbutyraldehyde; Lactobacillus sakei contains α-ketoacid decarboxylase, which promotes the catabolism of leucine to produce 3-methylbutyraldehyde. The present technical solution can improve the fermentation efficiency of naturally fermented sausages and regulate the flavor of the fermented sausages, so that the fermented fish sausages have a rich caramelized aroma, fruity flavor, and bacon flavor, and a delicate, smooth, and elastic taste.

The invention relates to the field of bioengineering, in particular to a mutant, an expression vector, a host and application thereof. The invention provides application of a mutation site as a target spot in preparation of alpha keto acid and / or L-glufosinate-ammonium. The mutation sites comprise one or more of the 52nd site, the 54th site, the 56th site, the 58th site, the 217th site, the 354th site and the 355th site of the D-amino acid oxidase. According to the invention, an active pocket of D-amino acid oxidase is modified, and a D-amino acid oxidase single-point or multi-point combined mutant with significantly enhanced catalytic activity on a catalytic substrate DL-glufosinate-ammonium is screened through multiple rounds of iterative saturated mutation. By utilizing the D-amino acid oxidase mutant provided by the invention, kinetic resolution of racemic glufosinate-ammonium is realized, and the D-amino acid oxidase mutant can be widely applied to production and application of L-glufosinate-ammonium.

The invention belongs to the technical field of biological medicines, and discloses application of beta-elemonic acid or derivatives thereof or pharmaceutically acceptable salts thereof in preparation of medicines for preventing and / or treating polycystic ovarian syndrome (PCOS). The beta-elemonic acid is screened from a medicinal and edible library, and experiments prove that the beta-elemonic acid can remarkably improve the PCOS ovarian form and the number of mature follicles, normalize the disordered estrus cycle, remarkably improve the serum hormone level and further remarkably improve the sugar tolerance, insulin tolerance and weight of PCOS. The beta-elemonic acid disclosed by the invention is a compound based on homology of medicine and food, can be used as a medicine as well as a natural ingredient of food, is relatively low in toxicity, ensures the safety after long-term use, is relatively low in safety evaluation cost of a relatively brand-new synthetic compound, and can be applied to development and preparation of a medicine or a lead compound thereof for preventing and / or treating PCOS, or a health care product.

The invention belongs to the technical field of gestational diabetes diagnosis marker screening, and particularly relates to a biomarker for predicting or diagnosing gestational diabetes and application of the biomarker. The invention discloses a biomarker for predicting or diagnosing gestational diabetes mellitus, the biomarker is selected from one or more of hypoxanthine, cortisol, dihydrotachysterol and cycloleucine, and more preferably, the biomarker comprises hypoxanthine, cortisol, dihydrotachysterol, (3R, 4R)-D-ketolactone, creatine, cycloleucine and L-hydroorotic acid. The seven biomarkers disclosed by the invention can predict or diagnose gestational diabetes mellitus independently or jointly through plasma detection at the early pregnancy stage, effectively improve the early diagnosis rate of gestational diabetes mellitus and improve disease prognosis, and have clinical use and popularization values.

The invention discloses a method for synthesizing thioamide through photocatalysis. According to the method, sulfur powder, ketonic acid and amine are taken as starting raw materials, and the thioamide compound is synthesized in a green and efficient manner through dehydration condensation, photooxidation reduction decarboxylation and coupling sulfur insertion at room temperature under blue light irradiation. The method designed by the invention is novel and efficient, the raw materials are green and odorless, the synthesis method is simple, the conditions are mild, the yield is higher, the substrate application range is wide, and the method has very strong popularization potential.

The invention belongs to the technical field of pesticides, and relates to a pesticide composition containing a diphenyl sulfide compound and application of the pesticide composition. The pesticide composition containing the diphenyl sulfide compound comprises an active component A and an active component B, the active component A is a diphenyl sulfide compound, and the active component B is any one or more insecticidal and acaricidal agents: B1 heterocycles, B2 tetronic acids, B3 benzoylacetonitrile, B4 oxazoles, B5 pyrazole amides and the like. The pesticide composition can effectively prevent and control multiple phytophagous pest mites, has a synergistic effect in a certain proportion range, and can enlarge the mite killing spectrum, reduce the use cost, reduce the dosage, prolong the lasting period and delay the development of drug resistance.

The invention discloses herbicidal activity application of unsaturated keto ester compounds, and belongs to the technical field of organic pesticides. The alkylamino-containing unsaturated keto ester compound can be used as an effective component for preparing a herbicide, and the prepared herbicide has a relatively good weed growth inhibition effect, is safe to commercial crops such as tomatoes, radishes and lettuce, and has a wide application prospect.

The invention discloses a Zymomonas mobilis recombinant strain for producing D-pantothenic acid as well as a preparation method and application of the Zymomonas mobilis recombinant strain. A hydroxymethyl transferase gene panB, a ketopantoic acid reductase gene panE and a pantothenic acid synthase gene panC are integrated in a genome of the recombinant strain; an expression plasmid containing an acetolactate synthase gene Bsals, a keto acid reductoisomerase gene ilvC and a dihydroxy acid dehydratase gene ilvD is also transferred into the recombinant strain; wherein the nucleotide sequences of the gene Bsals, the gene ilvC, the gene ilvD, the gene panB, the gene panE and the gene panC are shown as SEQ ID NO. 1 to SEQ ID NO. 6 in sequence. The zymomonas mobilis ZM4 is systematically modified through a genetic engineering means, and an optimal enzyme combination adaptive to the zymomonas mobilis is defined by screening hydroxymethyltransferase, pantothenic acid synthase and ketopantoic acid reductase from different sources, so that a core foundation is laid for efficient operation of a D-pantothenic acid synthesis route, and the method has a wide application prospect. The technical bottlenecks of single metabolic pathway and low synthesis efficiency of the traditional strain are broken through.

The invention relates to a novel spirocyclopropane tetronic acid derivative with insecticidal and acaricidal activity as well as preparation and application of the novel spirocyclopropane tetronic acid derivative. Specifically, the invention provides a compound as shown in a formula (I), and an optical isomer, a cis-trans isomer or a pharmaceutically acceptable salt thereof. The compound disclosed by the invention has obvious insecticidal and acaricidal activity, and can be used for developing novel crop insecticidal and acaricidal agents which are low in toxicity, efficient and environment-friendly. # imgabs0 #

The invention discloses a method for preparing a zalvizepam chiral intermediate by a chemical-enzyme method. According to the present invention, the aromatic hydrocarbon fragment of the compound III is directly removed through the concentrated sulfuric acid or the concentrated hydrochloric acid to obtain the ketonic acid compound IV, and then the ketonic acid compound IV reacts with the transaminase to obtain the zavazepam chiral intermediate I. The route has characteristics of simple operation and low cost, and is suitable for industrial production.

The application relates to a buffer reagent, a kit, a branched-chain alpha-keto acid or a branched-chain amino acid detection method in the technical field of biological detection. The buffer reagent comprises 15-25 mM, pH 8.5-9.5 Tris-HCl buffer solution, 10-50 g / L trehalose, 0.1-1.5 g / L Proclin 300, 0.1-0.5 g / L Triton X-100, 0.1-1.5 g / L sodium salt of EDTA, 1-10 kU / L ascorbic acid oxidase and 0.5-3 g / L bovine serum albumin. The buffer reagent is suitable for preparation of a reagent containing leucine dehydrogenase, the reagent containing the buffer reagent and the leucine dehydrogenase and the kit prepared by using the reagent can effectively eliminate interference problems in the process of being used for branched-chain alpha-keto acid, branched-chain amino acid detection, and has good correlation with mass spectrometry.

A skin care composition includes a plurality of agents that include at least one retinol derivative that may comprise, for example, hydroxypinacolone retinoate, and at least one bark extract that may comprise, for example, Eperua falcata bark extract, the plurality of agents providing reduced retinol associated inflammation as compared with a composition that includes the at least one retinol and lacks the at least one bark extract. The plurality of agents may also include hydroxy acid, for example, comprising lactic acid. The composition may confer one or a combination of increased skin cell proliferation, improved cellular migration, improved cellular differentiation, an anti-inflammatory retinol genetic signature as demonstrated by via bulk-RNA sequencing of human keratinocytes and restores epidermal disruption and reduce IL-1A and IL-23 inflammatory cytokines in human ex-vivo skin models of chronic inflammation.

The present disclosure pertains to improved methods of preparing high melting dicarboxylic acids or oxo-acids (e.g., carboxylic acid / aldehydes) by ozonolysis of their esters. For example, the present disclosure provides methods for preparing brassylic acid by way of the ozonolysis of erucic acid ester, the method comprising the steps of converting the erucic acid to an ester, performing ozonolysis on the ester, and converting the resulting brassylate ester product to brassylic acid.

The invention relates to the technical field of cosmetics, and particularly discloses anti-wrinkle cream containing sequoia stem fermentation products and a preparation method of the anti-wrinkle cream. The wrinkle removing cream containing the sequoia stem fermentation product is prepared from the following raw materials in percentage by mass: a material A: glycerol, butanediol, p-hydroxyacetophenone YS028, an ammonium acryloyldimethyl taurate / VP copolymer, p-hydroxyacetophenone YS008 and the balance of water; the material B is prepared from cetostearyl alcohol, an emulsifying agent MONTANOV202, butyrospermum parkii butter, isopropyl myristate, C10-18 fatty acid triglyceride, polydimethylsiloxane, ethylhexyl palmitate and XIAMETERPMX-0345Z Fluid; the material C is prepared from a material D and a material E; the material C is prepared from a sequoia stem fermentation product, Naturalpenet 2020 and SEPIGEL305; the material D is prepared from YONOME SPE II, Alps mountain leucas, ULA-L1004 coated ceramide, anti-wrinkle base peptide, super-oinmetidyl-D2, Chinese prescription hexyl A powder, XYREPIOR, annoine TM, essence and methyl dihydrojasmonate; the material D is prepared from YONOME SPE II, Alps mountain leucas, ULA-L1004 coated ceramide, anti-wrinkle base peptide, super-oinmetidyl-D2, Chinese prescription hexyl A powder, XYREPIOR, annoine TM In addition, the preparation method has the advantage of improving the wrinkle removing effect of the wrinkle removing cream.

The application belongs to the technical field of compound synthesis, and particularly relates to an alpha-ketoamide derivative and a preparation method thereof, wherein the preparation method of the alpha-ketoamide derivative comprises the following steps: S1, first, a graphite carbon rod electrode and a platinum sheet electrode are respectively assembled on two sides of a 10mL three-port glass bottle; S2, then, a magnetic son is added into the three-port glass bottle, the bottle mouths on two sides are sealed, the three-port glass bottle is transferred to a glove box for feeding, and benzoylformic acid, morpholine and 10mol% of ferrocene in 4mL hexafluoroisopropanol are sequentially added; S3, after the feeding is completed, the last bottle mouth of the three-port glass bottle is sealed, and a constant current of 3mA is introduced for electrolysis. The application realizes the amination reaction of alpha-keto acid by using an electrocatalytic decarboxylation strategy, selectively synthesizes alpha-ketoamide derivatives, and the reaction is suitable for primary amine compounds, secondary amine compounds, is also suitable for aromatic and aliphatic alpha-keto acids, and excellent functional group tolerance is exhibited.

This description describes a method for treating pain by administering a pharmaceutical composition containing a therapeutically effective amount of margaric acid to a subject requiring pain treatment. Pharmaceutical compositions, such as topical and transdermal compositions, containing margaric acid and a pharmaceutically acceptable excipient are also described. A pain-relieving composition containing margaric acid, eicosapentaenoic acid, and a pharmaceutically acceptable excipient is further described.

A skin care composition includes a plurality of agents that include at least one retinol derivative and at least one bark extract, the plurality of agents providing reduced retinol associated inflammation as compared with a composition that includes the at least one retinol and lacks the at least one bark extract. The plurality of agents may include at least one retinol derivative comprising hydroxypinacolone retinoate and at least one bark extract which may include at least one bark extract comprising Eperua falcata bark extract. The plurality of agents may also include at least one hydroxy acid, for example, comprising lactic acid. The composition confers one or more of increased skin cell proliferation, improved cellular migration, improved cellular differentiation, or a combination thereof. Application the plurality of agents confers to skin cells and tissues an anti-inflammatory retinol genetic signature as demonstrated by via bulk-RNA sequencing of human keratinocytes and restores epidermal disruption and reduce IL-1A and IL-23 inflammatory cytokines in human ex-vivo skin models of chronic inflammation.

This application provides a method for simultaneously detecting hydroxy acids and keto acids in a sample. The method includes the following steps: preparing a standard solution; performing ketone and carboxyl derivatization on the sample to be tested containing hydroxy acids and keto acids to obtain the sample to be tested; performing ketone and carboxyl derivatization on the standard solution to obtain a derivatized standard solution; and sequentially adding a ketone derivatization reagent and... 13 The method involves derivatization with a C6-labeled carboxyl derivatization reagent to obtain an isotope internal standard solution. This solution is then added to both the sample to be tested and the derivatized standard solution for LC-MS / MS analysis. The method described in this application achieves unified detection of paired hydroxy acids and keto acids by selectively derivatizing the ketone group followed by the carboxyl group. A sequential derivatization strategy is constructed based on the functional group differences between keto and hydroxy acids, significantly improving the stability, repeatability, and quantitative reliability of keto acid detection while enhancing overall detection sensitivity.

The present invention discloses a method for synthesizing hydroxytyrosol by multi-enzyme cascade, comprising: 1) preparing a hydroxytyrosol product by using 3,4-dihydroxybenzaldehyde, L-threonine, sodium formate, NAD + As raw material, L-threonine transaldolase, alcohol dehydrogenase, and formate dehydrogenase are used to catalyze the synthesis of L-threo-3-(3,4-dihydroxyphenyl)serine; 2) L-threo-3-(3,4-dihydroxyphenyl)serine is used as substrate, glucose, NAD + Hydroxytyrosol is synthesized through a cascade catalysis of phenylserine dehydratase, α-ketoacid decarboxylase, aldehyde reductase, and glucose dehydrogenase. The synthesis method provided by the present invention achieves a hydroxytyrosol yield of >99% and a space-time yield of 0.88 g / L / h, currently the highest, showing great potential for industrial application and suitable for further promotion and application.

The present application relates to the technical field of Fischer-Tropsch synthesis wax analysis chemistry, and in particular to a method for detecting oxygen-containing compound components in Fischer-Tropsch synthesis wax by using solid-phase microextraction and comprehensive two-dimensional gas chromatography-mass spectrometry. The method comprises the following steps: first, performing solid-phase microextraction on a to-be-tested wax sample to obtain an extract rich in oxygen-containing compound components; and then using comprehensive two-dimensional gas chromatography-mass spectrometry to qualitatively determine the composition of the oxygen-containing compound components contained in the extract. In the method, the content of the oxygen-containing compound components is less than or equal to 7 wt% based on the total weight of the Fischer-Tropsch synthesis wax. The method has the characteristics of high throughput, high sensitivity and high accuracy, and solves the problems of difficult analysis and separation and difficult qualitative determination of alcohol, aldehyde, ketone, acid, ester and other oxygen-containing compound components in Fischer-Tropsch synthesis wax.

The present invention discloses a meso-diaminopimelate dehydrogenase mutant of Proteus vulgaris and its application, belonging to the technical field of bioengineering. The present invention constructs a PvDAPDH mutant, which, compared with the wild enzyme, can catalyze the synthesis of D-amino acids from a variety of aromatic α-ketoacids, and the stereoselectivity can reach 95%. The triple mutant W121I / H227I / R181S of pvDAPDH constructed in the present invention shows good compatibility with different large-volume aromatic α-ketoacid substrates, can simultaneously catalyze the conversion of most aromatic α-ketoacid substrates into D-amino acids, shows high catalytic activity towards 2-oxo-4-phenylbutyric acid, and the yield of the product D-homophenylalanine reaches 2.1 g / L. The present invention effectively expands the toolbox for the biosynthesis of aromatic D-amino acids.

The invention relates to a fragrance composition for relieving mood of children as well as a preparation method and application of the fragrance composition. The fragrance composition is prepared from the following components in percentage by weight: 40 to 60 percent of methyl dihydrojasmonate, 30 to 50 percent of brazyl acid glycol cyclic ester, 1 to 10 percent of terpinyl dihydroacetate, 1 to 10 percent of tetrahydrolinalool and 1 to 5 percent of methyl cedryl ketone. Compared with the prior art, the emotional soothing fragrance has universality (can be used for cosmetics of various dosage forms), the emotional soothing effect is achieved by reducing the heart rate, breath and the like used by children, meanwhile, the brain wave alpha / beta power is improved, and the soothing and nerve soothing effect is achieved.

An electrochemical method for preparing N-arylsulfonylimine compound, comprising the following steps: in air atmosphere, a sulfoximine compound and an alpha-keto acid are added into a reactor in a molar ratio of 1:2, an electrolyte tetrabutylammonium tetrafluoroborate, a catalyst nickel bromide are added, and then an acetone solution is added; the mixture is stirred by a magnetic stirring device, and is dissolved; two electrodes are inserted, a graphite electrode is used as a positive electrode, and a platinum sheet electrode is used as a negative electrode; 6mA of current is passed, and the current passing time is 3h; after the reaction is completed, the solvent is removed by vacuum evaporation to obtain a crude product, and the N-arylsulfonylimine compound is obtained by column chromatography purification. Compared with a traditional synthesis method, the method has the advantages that the reaction condition is mild, can be successfully carried out at room temperature, operation is simple, all operations can be carried out in an open system, an electric current is used as an oxidation method, pollution of a chemical oxidant is avoided, raw materials are easy to obtain, functional group compatibility is good, and the scope of application of a substrate is wide.

The invention discloses a near-infrared two-window fluorescent probe for detecting viscosity as well as a preparation method and application thereof, belongs to the technical field of biological fluorescence imaging contrast agents, and provides a structural formula of a viscosity probe and a preparation method of the near-infrared two-window fluorescent probe for detecting viscosity. Comprising the following steps: S1, cyclohexanone and 4-diethylaminoketonic acid are subjected to a reaction in concentrated sulfuric acid, and a solid product (a compound 1) is obtained through post-treatment; and S2, carrying out a reaction on the compound 1 obtained in the step S1 and 9-aldehyde julolidine in an ethanol solution. And carrying out post-treatment to obtain solid R-CHO. The detection method disclosed by the invention has the advantages of simplicity and convenience in operation, rapidness, accuracy, high sensitivity and the like, and is suitable for biomedical research, clinical diagnosis and other related fields.

The application provides a synthesis method of 4-(hydroxymethyl phosphine) 2-carbonyl butyric acid, comprising the following steps: a) mixing acryloyl chloride, a first solvent, a polymerization inhibitor, a catalyst and sodium cyanide, performing a substitution reaction, and obtaining an acryloyl cyanide intermediate after reduced pressure distillation; b) mixing the acryloyl cyanide intermediate obtained in the step a) with hydrochloric acid and a polymerization inhibitor, performing a hydrolysis reaction, and obtaining 2-carbonyl-3-butenoic acid crude product after purification treatment; c) mixing the 2-carbonyl-3-butenoic acid crude product obtained in the step b) with a second solvent and methyl phosphinite, performing an addition reaction, recovering the solvent after water separation and extraction, and obtaining a ketonic acid ester solution; and d) adding hydrochloric acid to the ketonic acid ester solution obtained in the step c) to adjust the pH to be less than or equal to 3, performing a hydrolysis reaction, and obtaining 4-(hydroxymethyl phosphine) 2-carbonyl butyric acid after purification. The synthesis method selects inexpensive acryloyl chloride as a raw material, has mild reaction conditions, is simple to operate, has low production cost, is easy to realize industrialization, and has high yield.

A method for reducing fouling in a styrene production process, the method comprising the steps of introducing an additive into a stream containing styrene and a byproduct, divinylbenzene (DVB), wherein the additive comprises at least one compound comprising one or more functional groups selected from the group consisting of amines, alcohols, aminoalcohols, labile C-C, esters, carbamates, aldehydes, ketones, acids, acetates, benzoates, labile hydrogen, and combinations thereof, and having a boiling point greater than or equal to 170°C and within ±10°C, ±20°C, ±30°C, ±40°C, ±50°C, or ±60°C of the boiling point of divinylbenzene (DVB), which is 195°C, wherein the at least one compound is effective in inhibiting crosslinking of the divinylbenzene (DVB). A system for performing the method is also provided.

An oxidation strategy of benzyl C-H compounds, halides, alkenes, alkynes or alcohols mediated by butanedione, which can efficiently prepare alcohol, aldehyde, ketone or carboxylic acid compounds by using 1o, 2oor 3obenzyl C-H compounds, halides, alkenes, alkynes or alcohols as raw materials, adding butanedione, acid and solvent, and then irradiating under oxygen condition by visible light. The method provides an oxidation method for many natural products, drugs and materials, which does not need traditional oxidants, does not use transition metal catalysts, uses cheap butanedione as photosensitizer, uses water as solvent, and efficiently oxidizes 1o, 2oor 3obenzyl C-H compounds, halides, alkenes, alkynes or alcohols to prepare alcohol, aldehyde, ketone and carboxylic acid compounds under the induction of visible light. The whole production process is green, low-cost, wide-substrate applicability, high-yield, simple operation, no explosion risk, and has very significant advantages compared with the previous production process.

The invention relates to a super-crosslinking photosensitive material as well as a preparation method and application thereof. According to the photosensitive material, photosensitive molecules such as 2, 4, 5, 6-tetra (9-carbazolyl)-isophthalonitrile, 1, 3-dicyano-2, 4, 5, 6-tetra (diphenylamino)-benzene, anthraquinone, thioxanthone and the like are used as a basic structural unit as a skeleton; according to the preparation method, p-xylylene dichloride, 1, 4-di (bromomethyl) benzene, 1, 3, 5-tri (bromomethyl) benzene or biphenyl xylylene dichloride is used as a cross-linking agent, and photosensitive molecules are bonded with the cross-linking agent to obtain the super-crosslinked photosensitive material with the photosensitive property. The photosensitive material is applied to the asymmetric alpha-hydroxylation reaction of beta-keto ester, and an asymmetric alpha-hydroxylation reaction process with oxygen as an oxidizing agent is developed. The method provided by the invention is environment-friendly and has relatively high stereoselectivity.

Compositions and methods for the production of L-glufosinate are provided. The method involves converting racemic glufosinate to the L-glufosinate enantiomer or converting PPO to L-glufosinate in an efficient manner. In particular, the method involves the specific amination of PPO to L-glufosinate, using L-glutamate, racemic glutamate, or another amine source as an amine donor. PPO can be obtained by the oxidative deamination of D-glufosinate to PRO (2-oxo-4-(hydroxy (methyl) phosphinoyl) butyric acid) or generated via chemical synthesis. PPO is then converted to L-glufosinate using a transaminase in the presence of an amine donor. When the amine donor donates an amine to PPO. L-glufosinate and a reaction by product are formed. Because the PPO remaining represents a yield loss of L-glufosinate, it is desirable to minimize the amount of PPO remaining in the reaction mixture. Degradation, other chemical modification, extraction, sequestration, binding, or other methods to reduce the effective concentration of the by-product. i.e., the corresponding alpha ketoacid or ketone to the chosen amine donor will shift the reaction equilibrium toward L-glufosinate, thereby reducing the amount of PPO and increasing the yield of L-glufosinate. Therefore, the methods described herein involve the conversion or elimination of the alpha ketoacid or ketone by-product to another product to shift the equilibrium towards L-glufosinate.

The invention discloses simple synthesis and antibacterial application of amino alcohol spiro indoline. The invention provides a skeleton structure of amino alcohol spiro indoline. The invention provides a synthesis method of the amino alcohol spiro-indoline compound, which comprises the following steps: uniformly mixing indole and o-aminobenzoic acid ester in a solvent, reacting at 80 DEG C under an acidic condition, and preparing the amino alcohol spiro-indoline compound without separation and borane reduction after an intermediate is obtained. The invention also provides an application of the amino alcohol spiroindoline skeleton in the aspect of bacteriostasis, and the amino alcohol spiroindoline skeleton is used for preparing a bactericide. The invention provides a method for efficiently synthesizing amino alcohol spiro indoline through two-step continuous reaction.