108 results about "Keto acid" patented technology

The invention discloses herbicidal activity application of unsaturated keto ester compounds, and belongs to the technical field of organic pesticides. The alkylamino-containing unsaturated keto ester compound can be used as an effective component for preparing a herbicide, and the prepared herbicide has a relatively good weed growth inhibition effect, is safe to commercial crops such as tomatoes, radishes and lettuce, and has a wide application prospect.

The invention discloses a method for preparing a zalvizepam chiral intermediate by a chemical-enzyme method. According to the present invention, the aromatic hydrocarbon fragment of the compound III is directly removed through the concentrated sulfuric acid or the concentrated hydrochloric acid to obtain the ketonic acid compound IV, and then the ketonic acid compound IV reacts with the transaminase to obtain the zavazepam chiral intermediate I. The route has characteristics of simple operation and low cost, and is suitable for industrial production.

A skin care composition includes a plurality of agents that include at least one retinol derivative that may comprise, for example, hydroxypinacolone retinoate, and at least one bark extract that may comprise, for example, Eperua falcata bark extract, the plurality of agents providing reduced retinol associated inflammation as compared with a composition that includes the at least one retinol and lacks the at least one bark extract. The plurality of agents may also include hydroxy acid, for example, comprising lactic acid. The composition may confer one or a combination of increased skin cell proliferation, improved cellular migration, improved cellular differentiation, an anti-inflammatory retinol genetic signature as demonstrated by via bulk-RNA sequencing of human keratinocytes and restores epidermal disruption and reduce IL-1A and IL-23 inflammatory cytokines in human ex-vivo skin models of chronic inflammation.

The present disclosure pertains to improved methods of preparing high melting dicarboxylic acids or oxo-acids (e.g., carboxylic acid / aldehydes) by ozonolysis of their esters. For example, the present disclosure provides methods for preparing brassylic acid by way of the ozonolysis of erucic acid ester, the method comprising the steps of converting the erucic acid to an ester, performing ozonolysis on the ester, and converting the resulting brassylate ester product to brassylic acid.

The invention relates to the technical field of cosmetics, and particularly discloses anti-wrinkle cream containing sequoia stem fermentation products and a preparation method of the anti-wrinkle cream. The wrinkle removing cream containing the sequoia stem fermentation product is prepared from the following raw materials in percentage by mass: a material A: glycerol, butanediol, p-hydroxyacetophenone YS028, an ammonium acryloyldimethyl taurate / VP copolymer, p-hydroxyacetophenone YS008 and the balance of water; the material B is prepared from cetostearyl alcohol, an emulsifying agent MONTANOV202, butyrospermum parkii butter, isopropyl myristate, C10-18 fatty acid triglyceride, polydimethylsiloxane, ethylhexyl palmitate and XIAMETERPMX-0345Z Fluid; the material C is prepared from a material D and a material E; the material C is prepared from a sequoia stem fermentation product, Naturalpenet 2020 and SEPIGEL305; the material D is prepared from YONOME SPE II, Alps mountain leucas, ULA-L1004 coated ceramide, anti-wrinkle base peptide, super-oinmetidyl-D2, Chinese prescription hexyl A powder, XYREPIOR, annoine TM, essence and methyl dihydrojasmonate; the material D is prepared from YONOME SPE II, Alps mountain leucas, ULA-L1004 coated ceramide, anti-wrinkle base peptide, super-oinmetidyl-D2, Chinese prescription hexyl A powder, XYREPIOR, annoine TM In addition, the preparation method has the advantage of improving the wrinkle removing effect of the wrinkle removing cream.

This description describes a method for treating pain by administering a pharmaceutical composition containing a therapeutically effective amount of margaric acid to a subject requiring pain treatment. Pharmaceutical compositions, such as topical and transdermal compositions, containing margaric acid and a pharmaceutically acceptable excipient are also described. A pain-relieving composition containing margaric acid, eicosapentaenoic acid, and a pharmaceutically acceptable excipient is further described.

A skin care composition includes a plurality of agents that include at least one retinol derivative and at least one bark extract, the plurality of agents providing reduced retinol associated inflammation as compared with a composition that includes the at least one retinol and lacks the at least one bark extract. The plurality of agents may include at least one retinol derivative comprising hydroxypinacolone retinoate and at least one bark extract which may include at least one bark extract comprising Eperua falcata bark extract. The plurality of agents may also include at least one hydroxy acid, for example, comprising lactic acid. The composition confers one or more of increased skin cell proliferation, improved cellular migration, improved cellular differentiation, or a combination thereof. Application the plurality of agents confers to skin cells and tissues an anti-inflammatory retinol genetic signature as demonstrated by via bulk-RNA sequencing of human keratinocytes and restores epidermal disruption and reduce IL-1A and IL-23 inflammatory cytokines in human ex-vivo skin models of chronic inflammation.

This application provides a method for simultaneously detecting hydroxy acids and keto acids in a sample. The method includes the following steps: preparing a standard solution; performing ketone and carboxyl derivatization on the sample to be tested containing hydroxy acids and keto acids to obtain the sample to be tested; performing ketone and carboxyl derivatization on the standard solution to obtain a derivatized standard solution; and sequentially adding a ketone derivatization reagent and... 13 The method involves derivatization with a C6-labeled carboxyl derivatization reagent to obtain an isotope internal standard solution. This solution is then added to both the sample to be tested and the derivatized standard solution for LC-MS / MS analysis. The method described in this application achieves unified detection of paired hydroxy acids and keto acids by selectively derivatizing the ketone group followed by the carboxyl group. A sequential derivatization strategy is constructed based on the functional group differences between keto and hydroxy acids, significantly improving the stability, repeatability, and quantitative reliability of keto acid detection while enhancing overall detection sensitivity.

The present application relates to the technical field of Fischer-Tropsch synthesis wax analysis chemistry, and in particular to a method for detecting oxygen-containing compound components in Fischer-Tropsch synthesis wax by using solid-phase microextraction and comprehensive two-dimensional gas chromatography-mass spectrometry. The method comprises the following steps: first, performing solid-phase microextraction on a to-be-tested wax sample to obtain an extract rich in oxygen-containing compound components; and then using comprehensive two-dimensional gas chromatography-mass spectrometry to qualitatively determine the composition of the oxygen-containing compound components contained in the extract. In the method, the content of the oxygen-containing compound components is less than or equal to 7 wt% based on the total weight of the Fischer-Tropsch synthesis wax. The method has the characteristics of high throughput, high sensitivity and high accuracy, and solves the problems of difficult analysis and separation and difficult qualitative determination of alcohol, aldehyde, ketone, acid, ester and other oxygen-containing compound components in Fischer-Tropsch synthesis wax.

The invention relates to a fragrance composition for relieving mood of children as well as a preparation method and application of the fragrance composition. The fragrance composition is prepared from the following components in percentage by weight: 40 to 60 percent of methyl dihydrojasmonate, 30 to 50 percent of brazyl acid glycol cyclic ester, 1 to 10 percent of terpinyl dihydroacetate, 1 to 10 percent of tetrahydrolinalool and 1 to 5 percent of methyl cedryl ketone. Compared with the prior art, the emotional soothing fragrance has universality (can be used for cosmetics of various dosage forms), the emotional soothing effect is achieved by reducing the heart rate, breath and the like used by children, meanwhile, the brain wave alpha / beta power is improved, and the soothing and nerve soothing effect is achieved.

An electrochemical method for preparing N-arylsulfonylimine compound, comprising the following steps: in air atmosphere, a sulfoximine compound and an alpha-keto acid are added into a reactor in a molar ratio of 1:2, an electrolyte tetrabutylammonium tetrafluoroborate, a catalyst nickel bromide are added, and then an acetone solution is added; the mixture is stirred by a magnetic stirring device, and is dissolved; two electrodes are inserted, a graphite electrode is used as a positive electrode, and a platinum sheet electrode is used as a negative electrode; 6mA of current is passed, and the current passing time is 3h; after the reaction is completed, the solvent is removed by vacuum evaporation to obtain a crude product, and the N-arylsulfonylimine compound is obtained by column chromatography purification. Compared with a traditional synthesis method, the method has the advantages that the reaction condition is mild, can be successfully carried out at room temperature, operation is simple, all operations can be carried out in an open system, an electric current is used as an oxidation method, pollution of a chemical oxidant is avoided, raw materials are easy to obtain, functional group compatibility is good, and the scope of application of a substrate is wide.

The invention discloses a near-infrared two-window fluorescent probe for detecting viscosity as well as a preparation method and application thereof, belongs to the technical field of biological fluorescence imaging contrast agents, and provides a structural formula of a viscosity probe and a preparation method of the near-infrared two-window fluorescent probe for detecting viscosity. Comprising the following steps: S1, cyclohexanone and 4-diethylaminoketonic acid are subjected to a reaction in concentrated sulfuric acid, and a solid product (a compound 1) is obtained through post-treatment; and S2, carrying out a reaction on the compound 1 obtained in the step S1 and 9-aldehyde julolidine in an ethanol solution. And carrying out post-treatment to obtain solid R-CHO. The detection method disclosed by the invention has the advantages of simplicity and convenience in operation, rapidness, accuracy, high sensitivity and the like, and is suitable for biomedical research, clinical diagnosis and other related fields.

The application provides a synthesis method of 4-(hydroxymethyl phosphine) 2-carbonyl butyric acid, comprising the following steps: a) mixing acryloyl chloride, a first solvent, a polymerization inhibitor, a catalyst and sodium cyanide, performing a substitution reaction, and obtaining an acryloyl cyanide intermediate after reduced pressure distillation; b) mixing the acryloyl cyanide intermediate obtained in the step a) with hydrochloric acid and a polymerization inhibitor, performing a hydrolysis reaction, and obtaining 2-carbonyl-3-butenoic acid crude product after purification treatment; c) mixing the 2-carbonyl-3-butenoic acid crude product obtained in the step b) with a second solvent and methyl phosphinite, performing an addition reaction, recovering the solvent after water separation and extraction, and obtaining a ketonic acid ester solution; and d) adding hydrochloric acid to the ketonic acid ester solution obtained in the step c) to adjust the pH to be less than or equal to 3, performing a hydrolysis reaction, and obtaining 4-(hydroxymethyl phosphine) 2-carbonyl butyric acid after purification. The synthesis method selects inexpensive acryloyl chloride as a raw material, has mild reaction conditions, is simple to operate, has low production cost, is easy to realize industrialization, and has high yield.

An oxidation strategy of benzyl C-H compounds, halides, alkenes, alkynes or alcohols mediated by butanedione, which can efficiently prepare alcohol, aldehyde, ketone or carboxylic acid compounds by using 1o, 2oor 3obenzyl C-H compounds, halides, alkenes, alkynes or alcohols as raw materials, adding butanedione, acid and solvent, and then irradiating under oxygen condition by visible light. The method provides an oxidation method for many natural products, drugs and materials, which does not need traditional oxidants, does not use transition metal catalysts, uses cheap butanedione as photosensitizer, uses water as solvent, and efficiently oxidizes 1o, 2oor 3obenzyl C-H compounds, halides, alkenes, alkynes or alcohols to prepare alcohol, aldehyde, ketone and carboxylic acid compounds under the induction of visible light. The whole production process is green, low-cost, wide-substrate applicability, high-yield, simple operation, no explosion risk, and has very significant advantages compared with the previous production process.

The invention relates to a super-crosslinking photosensitive material as well as a preparation method and application thereof. According to the photosensitive material, photosensitive molecules such as 2, 4, 5, 6-tetra (9-carbazolyl)-isophthalonitrile, 1, 3-dicyano-2, 4, 5, 6-tetra (diphenylamino)-benzene, anthraquinone, thioxanthone and the like are used as a basic structural unit as a skeleton; according to the preparation method, p-xylylene dichloride, 1, 4-di (bromomethyl) benzene, 1, 3, 5-tri (bromomethyl) benzene or biphenyl xylylene dichloride is used as a cross-linking agent, and photosensitive molecules are bonded with the cross-linking agent to obtain the super-crosslinked photosensitive material with the photosensitive property. The photosensitive material is applied to the asymmetric alpha-hydroxylation reaction of beta-keto ester, and an asymmetric alpha-hydroxylation reaction process with oxygen as an oxidizing agent is developed. The method provided by the invention is environment-friendly and has relatively high stereoselectivity.

Compositions and methods for the production of L-glufosinate are provided. The method involves converting racemic glufosinate to the L-glufosinate enantiomer or converting PPO to L-glufosinate in an efficient manner. In particular, the method involves the specific amination of PPO to L-glufosinate, using L-glutamate, racemic glutamate, or another amine source as an amine donor. PPO can be obtained by the oxidative deamination of D-glufosinate to PRO (2-oxo-4-(hydroxy (methyl) phosphinoyl) butyric acid) or generated via chemical synthesis. PPO is then converted to L-glufosinate using a transaminase in the presence of an amine donor. When the amine donor donates an amine to PPO. L-glufosinate and a reaction by product are formed. Because the PPO remaining represents a yield loss of L-glufosinate, it is desirable to minimize the amount of PPO remaining in the reaction mixture. Degradation, other chemical modification, extraction, sequestration, binding, or other methods to reduce the effective concentration of the by-product. i.e., the corresponding alpha ketoacid or ketone to the chosen amine donor will shift the reaction equilibrium toward L-glufosinate, thereby reducing the amount of PPO and increasing the yield of L-glufosinate. Therefore, the methods described herein involve the conversion or elimination of the alpha ketoacid or ketone by-product to another product to shift the equilibrium towards L-glufosinate.

The invention discloses simple synthesis and antibacterial application of amino alcohol spiro indoline. The invention provides a skeleton structure of amino alcohol spiro indoline. The invention provides a synthesis method of the amino alcohol spiro-indoline compound, which comprises the following steps: uniformly mixing indole and o-aminobenzoic acid ester in a solvent, reacting at 80 DEG C under an acidic condition, and preparing the amino alcohol spiro-indoline compound without separation and borane reduction after an intermediate is obtained. The invention also provides an application of the amino alcohol spiroindoline skeleton in the aspect of bacteriostasis, and the amino alcohol spiroindoline skeleton is used for preparing a bactericide. The invention provides a method for efficiently synthesizing amino alcohol spiro indoline through two-step continuous reaction.

The invention belongs to the technical field of nano biological materials, and discloses a preparation method and application of a betulinic acid self-assembly nano composite system loaded with dihydromyricetin, and the nano composite system takes betulinic acid as a carrier and dihydromyricetin as an active component. The assembly mechanism of the prepared nano assembly system is as follows: the hydrogen bond interaction generated by combining the phenolic hydroxyl group of the dihydromyricetin molecule with the carbonyl group in the betulinic acid molecule and the carbonyl group in the carboxyl group in the betulinic acid molecule is the main non-covalent interaction force. The nano-composite system has good biocompatibility, and the HT-29 cells have a good uptake effect on the nano-composite system. The method has certain research and application prospects in the field of nano biological materials.

COMPOSITION SUITABLE FOR KERATINOUS FIBERS The present invention relates principally to a composition for keratinous fibers such as hair, comprising: (a) at least one compound selected from the group consisting of glyoxylic acid, glyoxylic acid solvates, glyoxylic acid salts, glyoxylic acid esters, glyoxylic acid amides, and mixtures thereof; (b) at least one compound, other than (a), selected from the group consisting of keto acids, their salts, and mixtures thereof; and (c) at least one quater ester. The present invention can improve the smoothing of keratinous fibers such as hair based on heating the keratinous fibers with glyoxylic acid or one of its derivatives, providing more durable remodeling effects. Figure for abstract: none

The invention relates to the technical field of chemistry and chemical engineering, in particular to an ultrasonic-promoted tembotrione acid preparation method which comprises the following steps: in the presence of inorganic base, performing substitution hydrolysis on bromide and trifluoroethanol in an organic solvent A under the promotion of ultrasonic waves; after the reaction is finished, distilling and recovering the organic solvent A, and if the organic solvent B is the same as the organic solvent A, omitting the distilling step; adding water and an organic solvent B into the distilled reaction product, if the organic solvent B is the same as the organic solvent A, not adding the organic solvent B, standing for layering, and extracting a water layer by using the organic solvent B; combining organic phases, and recycling and reusing by distillation; combining water phases, adjusting the pH value to 1-2 by using hydrochloric acid, filtering and washing to obtain a tembotrione acid product; according to the present invention, the reaction speed is improved so as to shorten the reaction time, improve the reaction selectivity, and reduce the impurity generation so as to improve the reaction yield;

The invention provides oleanolic acid, and a preparation method of the oleanolic acid comprises the following steps: S1, dissolving 5g of arginic acid in 500ml of dichloromethane, adding 7g of pyridinium dichlorochromate, stirring at room temperature for 18 hours, and detecting the reaction degree by thin-layer chromatography; s2, after the reaction is completed and vacuum concentration is carried out, a sample is mixed with 200-300-mesh silica gel, the mixture is volatilized to be dry and then placed on a silica gel column, column chromatography separation is carried out, petroleum ether / ethyl acetate is selected as a mobile phase, 2.87 g of oleanolic acid is obtained, the molecular formula is C30H46O3, and the yield is 57.6%. The oleanolic acid provided by the invention can be used for preparing medicines for treating oligospermia and medicines for improving dyszoospermia. The oleanolic acid provided by the invention is a natural product or an artificially synthesized product, is low in toxicity and small in side effect, and has good effects of improving dyszoospermia and promoting fertility.

A diaminopimelate dehydrogenase mutant and a use in D-amino acid synthesis. A diaminopimelate dehydrogenase mutant obtained by performing molecular modification on a Bacillus thermozeamaize diaminopimelate dehydrogenase, a nucleic acid encoding the diaminopimelate dehydrogenase mutant, a recombinant expression vector containing the nucleic acid, a recombinant expression transformant containing the recombinant expression vector, and a use of the diaminopimelate dehydrogenase mutant in the synthesis of a D-amino acid by means of the asymmetric reductive amination of a 2-keto acid. Compared with other D-biphenylalanine synthesis methods, the present invention has a simple route, a high theoretical yield, good atom economy, and a product optical purity of more than 99%. Therefore, the present invention relates to an environmentally-friendly, highly-stereoselective and green biological synthesis method, and has good prospects for application in actual production of D-biphenylalanine and other D-amino acids.

The invention discloses an application of a branched ketonic acid dehydrogenase kinase (BCKDK) inhibitor in preparation of a medicine for preventing and treating osteoporosis. The invention finds that BCKDK can inhibit osteoblast differentiation and reduce the expression of endogenous BCKDK in osteogenic precursor cells so as to promote osteoblast differentiation; a small molecule compound inhibitor BT2 (3, 6-dichloro-2-benzothiophene carboxylic acid) of BCKDK promotes osteoblast differentiation of ovariectomized mice, inhibits osteoclast differentiation, reduces ovariectomized mouse bone loss and increases bone mass. According to the invention, the BCKDK inhibitor is proposed for the first time to be capable of promoting osteoblast differentiation, inhibiting osteoclast differentiation and increasing body bone mass by reducing BCKDK expression or inhibiting BCKDK protein biological functions, and has the effect of preventing and treating osteoporosis.

The application provides a kinase inhibitor leading compound, a virtual screening method thereof and application of the kinase inhibitor leading compound in preparation of branched-chain alpha-keto acid dehydrogenase kinase inhibitors and / or drugs for treating diseases mediated by branched-chain alpha-keto acid dehydrogenase kinase. The virtual screening method provided by the application combines various virtual screening platforms to screen a large-scale compound library, improves the true positive rate of screening results, reduces the number of compounds needing experimental screening, and saves screening time and cost. In addition, the compounds I-III screened out with good binding affinity to BCKDK can be used in researches on targeting BCKDK to treat major diseases such as obesity, insulin resistance, maple syrup urine disease, neurological dysfunction, liver cancer, colorectal cancer and tumor cachexia.

The invention discloses a preparation method of methyl dehydrojasmonate, and belongs to the technical field of carbocyclic compounds, the method comprises the following steps: bromination reaction, debromination reaction and post-treatment; the bromination reaction method comprises the following steps: mixing dihydro jasmonic acid methyl ester alkenyl ester, a boron trifluoride diethyl etherate complex and a solvent, cooling the system to 9-11 DEG C after mixing is completed, dropwise adding a bromine solution into the mixed system, and controlling the bromine dropwise adding time to be 5.8-6.2 hours; after the bromine is dropwise added, 4-dimethylaminopyridine is added, the system temperature is kept at 9-11 DEG C, the reaction is continued for 3.8-4.2 h, and after the reaction is finished, a bromination reaction filtrate is filtered and collected. According to the methyl dehydrojasmonate prepared by the method disclosed by the invention, the purity of the product is 99.1 to 99.4 percent, and the final yield is 89.8 to 93.1 percent.

The invention discloses a fluorescent probe for detecting the viscosity of a cell membrane as well as a preparation method and application of the fluorescent probe, and belongs to the technical field of small molecule probes. The structural formula of the fluorescent probe is shown in the specification. The preparation method specifically comprises the following steps: (1) reacting 4-diethylaminoketonic acid and cyclohexanone in concentrated sulfuric acid, pouring into crushed ice, stirring, adding perchloric acid, stirring, standing and filtering; (2) dissolving 4-diethylaminobenzaldehyde in absolute ethyl alcohol, heating and refluxing, removing the solvent, and carrying out chromatography; and (3) dissolving benzotriazole-N, N, N ', N'-tetramethylurea hexafluorophosphate and N, N-diisopropylethylamine in N, N-dimethylformamide, stirring at normal temperature, adding a compound 43-(tetradecyl amino) propane-1-sulfonate, continuously stirring, removing the solvent, and carrying out chromatography to obtain the compound 43-(tetradecyl amino) propane-1-sulfonate. The fluorescent probe has excellent anchoring and imaging capabilities and viscosity-triggered near-infrared (NIR) fluorescence response characteristics, can be used for fluorescence imaging of cell membrane viscosity, and can also be used for viscosity imaging analysis of breast cancer mice.

The application belongs to the field of industrial catalysis, and particularly relates to a method for synthesizing 2-keto acid by alternation magnetic field assisted Fe3N catalytic oxidation of 2-hydroxy acid, wherein 2-hydroxy acid of formula 1 ( ), an oxidizing agent and Fe3N are mixed and catalytic oxidation is carried out under the assistance of an alternation magnetic field to obtain 2-keto acid of formula 2 ( ). The application innovatively finds that the alternation magnetic field assisted Fe3N catalytic oxidation of 2-hydroxy acid can cyclically synthesize 2-keto acid with high selectivity and high stability under mild conditions; thus, the industrial amplification production of 2-keto acid can be facilitated.

The invention discloses an artificial synthesis method and application of malonyl coenzyme A. An artificial synthesis route for synthesizing malonyl-CoA by taking beta-alanine (beta-ala) as a precursor is constructed by heterologous expression of aminotransferase and malonyl-CoA reductase: firstly, under the catalysis of transaminase, amino of beta-ala is transferred to alpha-keto acid (such as pyruvic acid, oxaloacetic acid or alpha-ketoglutaric acid and the like), and then the malonyl-CoA is obtained; an intermediate product 3-oxopropionic acid and corresponding amino acid are formed; and the malonyl-CoA is generated from the 3-oxopropionic acid under the action of the malonyl-CoA reductase. According to the method, the defects existing in a natural malonyl-CoA synthesis route, such as low carbon utilization rate, energy substance ATP consumption, greenhouse gas CO2 release, pyruvate dehydrogenase (PDH) of route enzymes and acetyl-CoA carboxylase (ACC) which are strictly regulated and controlled, are overcome, and high yield of products taking malonyl-CoA as a precursor, including light flavomycin, octanoic acid, phloroglucinol, pentadecheptaene, natamycin, spinosad and the like, is realized.

The invention discloses a beta-elemonic acid derivative and a preparation method and application thereof, and belongs to the technical field of biological medicine, the structural formula of the beta-elemonic acid derivative is shown in the specification, the R1 group is one of 4-H, 4-Cl, 4-OCH3 and 3, 4, 5-(OCH3) 3; and R2 is one of 4-H, 4-F, 4-Cl and 4-OCH3. Tetracyclic triterpenoid beta-elemonic acid is used as a lead compound, a 1, 2, 3-triazole structure with anticancer activity is introduced, and the generated beta-elemonic acid derivative has low toxicity to human normal hepatocytes, high selectivity to tumor cells and remarkable anti-proliferation effect.

Article side polymers useful in the packaging of transdermal patches are disclosed. These films do not absorb skin permeation enhancers that are contained in transdermal patches including keto acids such as levulinic acid or sulfoxides such as an alkyl sulfoxide, e.g., dimethyl sulfoxide. Using such polymeric film, multilayer pouch constructions can be manufactured which include other polymeric layers, such as aluminum foil for moisture and oxygen barrier properties, polyester for tear resistance and paper layers for optimal printing and design.