958 results about "Enol" patented technology

The present invention relates to the production of a non-transgenic plant resistant or tolerant to a herbicide of the phosphonomethylglycine family, e.g., glyphosate. The present invention also relates to the use of a recombinagenic oligonucleobase to make a desired mutation in the chromosomal or episomal sequences of a plant in the gene encoding for 5-enol pyruvylshikimate-3-phosphate synthase (EPSPS). The mutated protein, which substantially maintains the catalytic activity of the wild-type protein, allows for increased resistance or tolerance of the plant to a herbicide of the phosphonomethylglycine family, and allows for the substantially normal growth or development of the plant, its organs, tissues or cells as compared to the wild-type plant irrespective of the presence or absence of the herbicide.

The invention provides a non-naturally occurring microbial organism having a muconate pathway having at least one exogenous nucleic acid encoding a muconate pathway enzyme expressed in a sufficient amount to produce muconate. The muconate pathway including an enzyme selected from the group consisting of a beta-ketothiolase, a beta-ketoadipyl-CoA hydrolase, a beta-ketoadipyl-CoA transferase, a beta-ketoadipyl-CoA ligase, a 2-fumarylacetate reductase, a 2-fumarylacetate dehydrogenase, a trans-3-hydroxy-4-hexendioate dehydratase, a 2-fumarylacetate aminotransferase, a 2-fumarylacetate aminating oxidoreductase, a trans-3-amino-4-hexenoate deaminase, a beta-ketoadipate enol-lactone hydrolase, a muconolactone isomerase, a muconate cycloisomerase, a beta-ketoadipyl-CoA dehydrogenase, a 3-hydroxyadipyl-CoA dehydratase, a 2,3-dehydroadipyl-CoA transferase, a 2,3-dehydroadipyl-CoA hydrolase, a 2,3-dehydroadipyl-CoA ligase, a muconate reductase, a 2-maleylacetate reductase, a 2-maleylacetate dehydrogenase, a cis-3-hydroxy-4-hexendioate dehydratase, a 2-maleylacetate aminoatransferase, a 2-maleylacetate aminating oxidoreductase, a cis-3-amino-4-hexendioate deaminase, and a muconate cis/trans isomerase. Other muconate pathway enzymes also are provided. Additionally provided are methods of producing muconate.

The invention relates to a catalyst used in a ring-opening hydrogenation reaction of a furan derivative. The catalyst is applied to direct preparation of one-step ring-opening hydrogenation of 1,5-pentanediol and 1,2-pentanediol by taking furfural or furfuryl alcohol serving as a raw material under a mild condition. The catalyst can provide two active ingredients, namely the ring-opening active center of a transition metal oxide and the hydrogenation active center of Pt, Pd, Rh, Ru, Co or Ni, wherein the active center of the transition metal oxide is mainly used for adsorbing furfural or furfuryl alcohol and directly hydrogenating a furan ring for opening the furan ring; and the hydrogenation active center of a noble metal or Co, Ni and the like is mainly used for quickly hydrogenating an intermediate material and hydrogenating subsequent enol so as to obtain 1,5-pentanediol and 1,2-pentanediol. An environmentally-friendly, reproducible, low-cost, mild and effective method is provided for producing 1,5-pentanediol and 1,2-pentanediol. The high-performance ring-opening hydrogenation catalyst is also suitable for the ring-opening hydrogenation reaction of other furan derivatives.

The present invention relates to the production of a non-transgenic plant resistant or tolerant to a herbicide of the phosphonomethylglycine family, e.g., glyphosate. The present invention also relates to the use of a recombinagenic oligonucleobase to make a desired mutation in the chromosomal or episomal sequences of a plant in the gene encoding for 5-enol pyruvylshikimate-3-phosphate synthase (EPSPS). The mutated protein, which substantially maintains the catalytic activity of the wild-type protein, allows for increased resistance or tolerance of the plant to a herbicide of the phosphonomethylglycine family, and allows for the substantially normal growth or development of the plant, its organs, tissues or cells as compared to the wild-type plant irrespective of the presence or absence of the herbicide. The present invention also relates to a non-transgenic plant cell in which the EPSPS gene has been mutated, a non-transgenic plant regenerated therefrom, as well as a plant resulting from a cross using a regenerated non-transgenic plant having a mutated EPSPS gene. The amino acids at the positions 126, 177, 207, 438, 479, 480 and/or 505 are changed tp produce a mutant EPSPS gene product.

The invention provides a novel empoasca vitis gothe attractant formula and application of the novel empoasca vitis gothe attractant. The application method comprises the following steps: mainly preparing the attractant by the following components including tea tree volatile matters trans-2-hexenal, cis-3-hexene-1-ol and cis-3-hexenyl acetate, geraniol, linalool and trans-ocimene as components of the attractant and an additional antioxidant 2, 6-butylated hydroxytoluene (BHT) in a weight ratio of (0.5-5.0):(10-20):(1.0-15):(5.0-15):(30-40):(1.0-15):(1.0-10); loading the attractant on a silicone rubber head to prepare a lure; and embedding the lure on a yellow sticky colored card to form a trap of empoasca vitis gothe. Imago of empoasca vitis gothe is trapped in a stable stage of trapping, preventing and treating the empoasca vitis gothe, so that the quantity of the empoasca vitis gothe can be effectively restricted. Researches show that the lure with the novel empoasca vitis gothe attractant can obviously enhance the trapping and killing effects of a single yellow sticky colored card, the lasting trapping capacity of the trap to the empoasca vitis gothe can be remarkably improved, the service period of the trap is remarkably prolonged, and use of chemical pesticides in the tea garden is remarkably reduced and cancelled. The attractant applied to the tea garden has the advantages of convenience in use, safety, greenness and no pollution and meets the national green prevention and control principle, thereby accelerating the industrial development of organic tea gardens.

This invention is directed to a method of using a therapeutic composition comprising a compound of an alpha-ketoalkanoic acid (pyruvate) and/or its derivatives for the treatment of cytokine-mediated inflammatory conditions. The compound is an alpha-ketoalkanoic acid, a physiologically acceptable salt of an alpha-ketoalkanoic acid, an ester of an alpha-ketoalkanoic acid, or an amide of an alpha-ketoalkanoic acid. A component for inducing and stabilizing the enol resonance form of the ester at physiological pH values is also disclosed. The cytokine-mediated inflammatory conditions are mediated by, for example, an “early” (Tumor Necrosis Factor (TNF), interleukin-1β (IL-1β)) or “late” (high mobility group B-1 (HMGB-1)) mediator of inflammation. Exemplary cytokine-mediated inflammatory conditions include, but are not limited to, local and systemic inflammation, inflammatory bowel disease (Crohn's disease and ulcerative colitis), rheumatoid arthritis, asthma (including status asthmaticus), sepsis or septic shock, also including inflammatory skin conditions, for example, psoriasis and eczema.

A chemical mechanical polishing composition contains 1) water, 2) optionally an abrasive material, 3) an oxidizer, preferably a per-type oxidizer, 4) a small amount of soluble metal-ion oxidizer/polishing accelerator, a metal-ion polishing accelerator bound to particles such as to abrasive particles, or both; and 5) at least one of the group selected from a) a small amount of a chelator, b) a small amount of a dihydroxy enolic compound, and c) a small amount of an organic accelerator. Ascorbic acid in an amount less than 800 ppm, preferably between about 100 ppm and 500 ppm, is the preferred dihydroxy enolic compound. The polishing compositions and processes are useful for substantially all metals and metallic compounds found in integrated circuits, but is particularly useful for tungsten. The present invention also pertains to surface-modified colloidal abrasive polishing compositions and associated methods of using these compositions, particularly for chemical mechanical planarization, wherein the slurry comprises low levels of chelating free radical quenchers, non-chelating free radical quenchers, or both.

The present invention includes a process for the manufacture of dihydrothebaine, dihydrocodeinone enol acetate, hydrocodone, and analogs thereof by reacting dihydrocodeine or analogs thereof with benzophenone in the presence of potassium tert-alkylate in a hydrocarbon solvent to generate a reaction mixture containing an enolate of the corresponding ketone, followed by addition of the reaction mixture to the electrophilic agent and isolation of the product.

Disclosed are preparing processes for industrialized application of Corosolic acid with blood sugar lowering, weight reducing, anti-tumor and anti-inflammatory actions and maslinic acid with anti-tumor, anti-virus and cardiovascular diseases resistant actions. The processes include respectively preparing Corosolic acid and maslinic acid using 3-carbonyl ursolic acid ester and 3-carbonyl oleanolic acid ester by acid catalysed enolization esterification reaction, hydroboration oxidation reaction, catalytic hydrogenolysis reaction and haloidlysis reaction.

The invention provides an antistatic surface protection film, the pollution to a pasted body is light, and the antistatic surface protection film is provided with excellent anti-stripping electrostatic properties without time dependent degradation. The antistatic surface protection film is formed in a way that a binder layer (2) composed of a binder composition is formed on one side of a base film (1) formed by a transparent resin, the binder composition comprises an acrylate-based polymer containing copolymers, (F) an isocyanate compound containing more than two functional groups, (G) a crosslinking accelerator and (H) a ketone-enol tautomer compound, a stripping agent layer (4) containing an antistatic agent is laminated on one side of a resin film (3) to form a stripping film (5), and the stripping film (5) is attached to the surface of the binder layer (2) through the stripping agent layer (4). The stripping agent layer (4) is formed by a resin composition, and the resin composition comprises a stripping agent taking dimethyl polysiloxane as the key component, a polysiloxane compound which is liquid under 20 DEG C and an antistatic agent, and the stripping electrostatic pressure of the binder layer (2) is lower than +/-0.6kV.

The invention discloses a synthesis process of vecuronium bromide. The synthesis process comprises the following steps: generating epiandrosterone sulfonyl ester (III) by carrying out esterification reaction between epiandrosterone (II) and paratoluensulfonylchloride; generating 5Alpha-androst-2-alkene-17-ketone (IV) by carrying out elimination and dehydration reaction between the (III) and 2,6-lutidines; generating 17-acetoxyl-5Alpha-androstane-2,16-diene (V) by carrying out enolization and esterification reaction between the (IV) and isopropenyl acetate; generating (2Alpha, 3Alpha, 16Alpha,17Alpha)-diepoxy-17Beta-acetyl-5Alpha-androstane (VI) by epoxy reaction of the (V) under the effect of hydrogen peroxide; generating 2Beta, 16Beta-di(1-piperidyl)-5Alpha-androstane-3Alpha-hydroxyl-17-ketone (VII) by ring-opening and addition reaction of the (VI) under the effect of hexahydropyridine; generating 2Beta, 16Beta-di(1-piperidyl)-5Alpha-androstane-3Alpha,17Beta-diol (VIII) by the (VII)under the reduction of potassium borohydride; generating 2Beta, 16Beta-di(1-piperidyl)-3Alpha, 17Beta- acetoxyl-5Alpha-androstane (IX) by carrying out esterification reaction of the (VIII) under the acetylation of acetic anhydride; and generating vecuronium bromide (I) by carrying out quaternary ammonium salt reaction between the (IX) and bromomethane. The invention has the advantages of low cost,less pollution and high yield.

The invention relates to the technical field of a preparation method of an antithrombotic drug Prasugrel. The preparation method utilizes the existing cyclopropyl-2- fluoro-benzylone which is treated with enol esterification, oxidization, sulfonylation, condensation and synthesis to obtain the Prasugrel. Compared with the prior art, the preparation method provided by the invention has low toxicity in raw materials and reagents used, low requirements in labor protection, low prices of and easy access to raw materials and reagents, less emission of three wastes (waste gas, waste water and waste residues), less pollution to the environment and no corrosion to equipment during the production; simultaneously, the preparation method also has simple operation and greater yield compared with the prior art and is applicable to industrial mass production.

According to the present invention, the oxidation agent uses air or oxygen, or mixture gas of oxygen and inert gas, or other chemical oxidation agent, the reaction pressure is controlled to be 1-10 atm, the temperature being 20-150 deg. C, mu-oxygen single metalloporphyrin or double metalloporphyrin, or their carrier is separately selected as catalyst, or they form composite catalyst along with filtering metallic salts or oxidants, the catalyst concentration being 1-50PPM, reaction time being 0.5-5 hours, thus alpha, beta-enol, alpha, beta-ketene and epoxy compounds are obtained through catalytic oxidation of olefin or cyclomonoolefin.

The invention relates to novel anti-tumor application of an alkaloid compound penicillium enol A1 from penicillium citrinum. The penicillium citrinum IBPT-5 is collected in the China Center for Type Culture Collection (CCTCC), which is located in Wuhan University and has the collection number of CCTCC NO:M2013713, on December 25, 2013. Experiments prove that the compound has better anti-tumor activity on various tumor cells, and can be used for preparing cell proliferation inhibition medicines or anti-tumor medicines for anti-tumor study, wherein tumor cells comprise human colon cancer cells SW620, human hepatoma cells Huh7, human gastric carcinoma cells BGC-823, human colon cancer cells SW480, human esophageal squamous carcinoma cells KYSE450, human esophageal cancer cells EC9706, human highly metastatic lung carcinoma cells 95-D, human hepatoma cells PLC and human gastric carcinoma cells HGC-27.

The invention relates to a synthesis method of methyl-alpha-fluoroacrylate and analogues thereof. The method comprises the following steps: step 1, based on methyl fluoroacrylate and dimethyl oxalate serving as raw materials, dissolving the raw materials in a solvent, and adding base for carrying out condensation reaction so as to generate sodium enolate or potassium enolate intermediate; and step 2, reacting sodium enolate or potassium enolate intermediate with paraformaldehyde or trioxane in the solvent so as to generate methyl-alpha-fluoroacrylate. According to the invention, cheap methyl fluoroacrylate and dimethyl oxalate are used as the raw materials, and the low-cost solvent and other reactants are selected, thus the method has the advantages of low preparation cost and high yield and can be suitable for large-scale industrial production.

The invention relates to application of a bacillus subtilis in the degradation of mycotoxins deoxynivalenol enol. The application method is as follows: 1. a method for eliminating the interference of a liquid medium to an indirect competitive ELISA which detects the deoxynivalenol enol; 2. a method for preparing an enzyme preparation from a strain cultured product; 3. a method for preparing a living bacteria preparation; and 4. a method for preparing an oral preparation or a drug for human body or animal body detoxification. The invention has the advantages that the bacillus subtilis can completely degrade the polluted mycotoxin deoxynivalenol enol in food or feed through biosynthesis and zymological approach under a moderate condition, and can avoid the disadvantages of incomplete degradation and destruction of nutrient caused by using a physical or a chemical method to process mycotoxin.

This invention has disclosed a kind of synthetic method to paclitaxol and side chain of its analogue. This invention using low-cost alpha- glycolic acid as raw material, the asymmetry addition reaction induced by chirality tertiary butyl sulfinamide as committed step, prepares paclitaxol and polyene paclitaxol side chain. The chemical product quotiety of key reaction is high, protecting group is easy to control and operate, synthesis process is short.