Novel anti-tumor application of penicillium enol B1 from penicillium citrinum

A technology of Penicillium citrinum and methanol, applied in the direction of antineoplastic drugs, methods based on microorganisms, medical preparations containing active ingredients, etc., to achieve significant antitumor activity

Inactive Publication Date: 2014-06-18
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Studies have found that the alkaloid compounds shown have anti-tumor activity, but there is no known effect of this compound on human colon cancer cells SW620, human liver cancer cells Huh7, human gastric cancer cells SGC-7901, human gastric cancer cells BGC-823, human colon cancer cells SW480, Human esophageal cancer cell EC9706, human highly metastatic lung cancer cell 95-D, human liver cancer cell PLC, human gastric cancer cell HGC-27, and human lymphoma cell line RAJI have reported cell proliferation inhibitory activity, so there is no related product in the market. drug

Method used

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  • Novel anti-tumor application of penicillium enol B1 from penicillium citrinum
  • Novel anti-tumor application of penicillium enol B1 from penicillium citrinum
  • Novel anti-tumor application of penicillium enol B1 from penicillium citrinum

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Embodiment 1

[0020] Fermentative production and separation and purification of the compound of embodiment 1

[0021] 1 Fermentation production

[0022] Fermentation culture of production bacteria: according to the conventional method of cultivating microorganisms, take Penicillium citrinum ( Penicillium citrinum ) IBPT-5 (preserved in China Center for Type Culture Collection on December 30, 2013, address: Wuhan University, Wuhan, deposit number: CCTCC NO: M 2013713) appropriate amount, inoculated on PDA solid slant medium at 28 Cultivate in an incubator for 4 days.

[0023] Get the Penicillium citrinum ( Penicillium citrinum. ) appropriate amount of IBPT-5, inoculated into 400mL culture medium [Culture medium composition (g / L): mannitol 20.0, yeast extract 3.0, maltose 20.0, monosodium glutamate 10.0, glucose 10.0, KH 2 PO 4 0.5 , MgSO 4 0.3, NaCl 30.0 constant volume] in a 1000mL Erlenmeyer flask, after 30 days of static culture at 28°C, the mycelia and fermentation broth were obt...

Embodiment 2

[0033] Example 2 Test of anti-tumor activity in vitro

[0034] 1 Experimental samples and experimental methods

[0035] Preparation of the tested sample solution The test sample is the pure product of the compound separated and refined in the above-mentioned implementation 1. Accurately weigh an appropriate amount of sample, and prepare a solution with the required concentration with methanol for activity measurement.

[0036] Cell lines and subculture of cells Tumor cell lines were used, and tumor cells were subcultured in DMEM medium containing 10% FBS at 37°C in an incubator filled with 5% carbon dioxide.

[0037] Cell Proliferation Inhibitory Activity Test Method

[0038] Tetrazolium salt (MTT) method Take tumor cells in the logarithmic growth phase and adjust the cell density to 2×10 per ml 5 Cells were inoculated into 96-well cell culture plates at 200 microliters per well, and filled with 5% CO at 37°C 2for 4 hours in an incubator. Add 2 microliters of sample solut...

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Abstract

The invention relates to novel anti-tumor application of an alkaloid compound penicillium enol B1 from penicillium citrinum. The penicillium citrinum IBPT-5 is collected in the China Center for Type Culture Collection (CCTCC), which is located in Wuhan University and has the collection number of CCTCC NO:M2013713, on December 25, 2013. The compound has good anti-tumor activity to various tumor cells, and can be used for preparing cell proliferation inhibition medicines or anti-tumor medicines for anti-tumor study, wherein tumor cells comprise human colon cancer cells SW620, human hepatoma cells Huh7, human gastric carcinoma cells SGC-7901, human gastric carcinoma cells BGC-823, human colon cancer cells SW480, human esophageal cancer cells EC9706, human highly metastatic lung carcinoma cells 95-D, human hepatoma cells PLC, human gastric carcinoma cells HGC-27 and human lymphoma cells RAJI.

Description

technical field [0001] The invention relates to a new anti-tumor application of an alkaloid compound penicramol B1 derived from Penicillium citrinum. Background technique [0002] Alkaloids are a type of nitrogen-containing organic compounds produced by biological secondary metabolism. There are many types of alkaloids in nature, most of which come from plants, so they are also called plant alkaloids. Alkaloids have important physiological effects on humans and animals, including anti-asthma and antitussive, hypoglycemic, hypolipidemic, antibacterial, antitumor, analgesic, etc., among which antibacterial and antitumor activities are the most prominent. Natural structural alkaloids are an important source for discovering lead compounds in the research of innovative drugs, and there are nearly a hundred kinds of alkaloid drugs currently used in clinical practice. Studies have found that some marine fungi can produce alkaloids with novel structures and good activities in the p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C07D207/02C12P17/10A61K31/4015A61P35/00C12R1/80
Inventor 陈立周彤宫美维张其清
Owner FUZHOU UNIV
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