Active carboxymethylpachymaran, production process and application thereof

Abstract

The invention relates to an industrialized production process of active carboxymethylpachymaran. Firstly, carrying out superfine grinding on the tuckahoe sclerotium to obtain tuckahoe superfine powder; extracting the superfine powder with edible alcohol at normal temperature, filtering to obtain filter cakes; taking the mixture of water and alcohol as a medium, wherein the volume ratio of alcohol to water is 15-10:1; and carrying out reaction between the filter cakes, chloroacetic acid and excessive sodium hydroxide to obtain the carboxymethylpachymaran with anti-tumor activity. The carboxymethylpachymaran obtained by the invention has high purity, favorable water solubility, moderate molecular weight, moderate substitution degree of carboxymethyl and obvious anti-tumor activity. The active carboxymethylpachymaran can be applied to preparing antitumor drugs or health foods. The preparation route of the invention is economic and environment-friendly, and is suitable for large-scale industrialized production.

Description

technical field [0001] The invention belongs to the field of anti-tumor macromolecular polysaccharides, and in particular relates to carboxymethylpachypolysaccharide with a medium degree of carboxymethyl substitution as a biological response regulator and its low-cost production process and application. Background technique [0002] Finding general and effective anticancer drugs and methods is a research hotspot in medicine and related fields. A large number of studies at home and abroad have proved that polysaccharides of traditional Chinese medicine have strong immune regulation and anti-tumor effects. Poria polysaccharide (Pachyman) is the main composition and active ingredient of the dry sclerotia of Poria cocos (Poria cocos), and its structure is (1→3) with a small amount of (1→6) branched chains. )-β-D-glucan. It has the natural advantages of natural polysaccharides such as good biocompatibility, low toxicity, biodegradability and edibility. Poria has a long history...

AI Technical Summary

Problems solved by technology

The extraction rate of the boiling water method is extremely low, the extraction time is long and the post-treatment workload is large, the yield of effective active substances of Poria cocos after decoction is only 1%, and the excessively high extraction temperature will also destroy the structure and activity of polysaccharides; The alcohol precipitation method requires low-temperature operation and relatively concentrated lye, and the post-treatment is difficult, time-consuming, and consumes a large amount of organic solvents such as ethanol and acetone; in addition, the polysaccharide itself has high molecular weight, poor solubility, viscosity and long-chain structure. The characteristics will also lead to complex preparation procedures of active carboxymethylpachyran, high viscosity of the reaction system, easy agglomeration of polysaccharides and difficult handling, and difficult separation of products

The above-mentioned shortcomings lead to the high production cost of carboxymethyl pachyrgan, polluting the environment and being difficult to refine, making it difficult to realize its large-scale production

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