176 results about "Marine fungi" patented technology

The present invention relates to the industrial application of ocean fission chytrid OUC88. The ocean fission chytrid OUC88 has capacity of generating rich docosahexaenoic acid (DHA), and has the preservation number of CGMCC No. 1240. The ocean fission chytrid OUC88 is new strain obtain with fission chytrid SR21 as originating strain and through chemical induction mutation and physical induction mutation. Through optimizing culture condition and replenishing carbon source in later culture stage, the present invention has dry cell weight reaching 25 g / L and DHA product up to 8.27 g / L. The thallus may be used as the additive for bread, mild product and other food products, especially baby's milk powder and can promote body's growth. The present invention has simple thallus fermenting process, high bioavailability, high yield of destination product and low cost.

The invention provides schizochytrium LX0809 (marine fungus), which has a high docosahexaenoic acid (DHA) production capability. The schizochytrium LX0809 of the invention was separated from the corroded soil collected from the red beach in Zhaoquanhe Town, Dawa County, Liaoning Province and was preserved in China General Microbiological Culture Collection Center with a preservation number CGMCC No.3535 on December 23th, 2009. When the schizochytrium LX0809 is used for fermenting supplementary food, the dry cell weight of the fermentation liquor can reach 72.5 g / L, and the DHA content can reach 15.3g / L. LX 0809 thallus cells and extracts thereof can be used as food additives for edible oil, dairy products, grain products and the like and can be used as a feed additive for aquaculture and livestock culture and the like. Saturated triglycercide in the thallus cells can be used to produce biodiesel through catalytic transesterification.

The present invention relates to the industrial application of ocean fission chytrid OUC88. The ocean fission chytrid OUC88 has capacity of generating rich docosahexaenoic acid (DHA), and has the preservation number of CGMCC No. 1240. The ocean fission chytrid OUC88 is new strain obtain with fission chytrid SR21 as originating strain and through chemical induction mutation and physical induction mutation. Through optimizing culture condition and replenishing carbon source in later culture stage, the present invention has dry cell weight reaching 25 g / L and DHA product up to 8.27 g / L. The thallus may be used as the additive for bread, mild product and other food products, especially baby's milk powder and can promote body's growth. The present invention has simple thallus fermenting process, high bioavailability, high yield of destination product and low cost.

The invention relates to a preparation method and application of a marine fungi aspergillus terreus butyrolactone compound butyrolactone-I. The invention discloses application of the marine fungi aspergillus terreus butyrolactone compound butyrolactone-I as shown in a formula (I) to preparation of medicaments for resisting to peripheral and neurogenic inflammation and resisting to neurodegenerative diseases. The formula is shown in the description. The invention finds that besides a DPPH free radical, butyrolactone-I can also well remove an ABTS free radical and an OH free radical; the marinefungi aspergillus terreus butyrolactone compound butyrolactone-I has better oxidation resistance and inflammation resistance and has better neuroprotection activity; therefore, the marine fungi aspergillus terreus butyrolactone compound butyrolactone-I has a wide application prospect in the aspect of preparing the medicaments for resisting to peripheral and neurogenic inflammation and resisting toneurodegenerative diseases. Meanwhile, by the preparation method of the butyrolactone-I, which is provided by the invention, the butyrolactone-I can be successfully prepared; the method is simple andeasy to realize large-scale production; culture conditions can also be optimized by addition of an inducer, so that yield of the butyrolactone-I is greatly improved.

The invention discloses a novel marine fungus-derived skeleton meroterpenoid derivative and an application thereof in preparation of an anti-inflammatory drug. According to the invention, the marine fungus, namely Aspergillus terreus GZU-311 is obtained through separation and screening; and the strain is preserved in the Guangdong Microbial Culture Collection Center on December 17th, 2019, and hasa preservation number of GDMCC No: 60789. Moreover, a meroterpenoid derivative is separated and identified from a fermentation culture product of the fungus; the meroterpenoid derivative has high NOproduction resisting activity; the anti-inflammatory activity of the meroterpenoid derivative is remarkably improved by about 2 times compared with the anti-inflammatory activity of a positive controlindomethacin; meanwhile, the meroterpenoid derivative has a high safety coefficient; thus, the meroterpenoid derivative provided by the invention has good clinical application prospect in preparationof anti-inflammatory drugs, specifically in preparation of drugs for inhibiting LPS-induced NO production.

The invention relates to marine fungi aspergillus terreus C23-3, a fermentation liquor active extract as well as a preparation method and application thereof. The marine fungi aspergillus terreus C23-3 is collected in the Guangdong Microbiological Culture Collection Center (GDMCC) on Jan 22th, 2018 with the collection number GDMCC No. 60316. Active substances of standing fermentation liquor of theaspergillus terreus C23-3 are subjected to extraction and purification; a fermentation liquor total extract of a bacterial strain can well resist oxidization and restrain the activity of acetylcholinesterase; an active extract prepared by purifying the fermentation liquor of the bacterial strain has very high oxidization resistance and / or acetylcholin esterase restraining activity, and has a very good application prospect on the aspect of preparation of an anti-senile dementia medicament and on the aspect of preparation of an anti-oxidization and / or acetylcholin esterase activity restrainingmedicament.

The invention discloses isobenzazole compounds derived from marine fungi, a preparation method of the isobenzazole compounds and application of the isobenzazole compounds in the preparation of anti-inflammatory drugs. The structural formulae of the isobenzazole new compounds are represented by a formula I, II, III or IV. The new compounds are capable of remarkably inhibiting the generation of LPS-induced NO and can be used for preparing the anti-inflammatory drugs, and the IC50 of the compounds are respectively 8.9mM(I), 12.4mM(II), 18.2mM(III) and 22.7mM(IV). Therefore, the isobenzazole compounds have the clinical application potential in the anti-inflammatory treatment.

The invention discloses a liquid fermentation method for producing laccase by using marine endogenous pestalotia, which comprises the following steps of: (1) screening a strain: screening soil in the depth of 10-20m on the offshore of the East China Sea to obtain a novel marine fungi; (2) preserving the strain: inoculating the strain in a modified potato dextrose agar (PDA) medium for culture, and preserving on a slope at the temperature of 4DEG C; (3) activating and propagating the strain: inoculating a spore of the strain in a PDA medium added with special components, and inoculating the activated spore in a seed culture medium for propagation; (4) preparing a marine endogenous pestalotia liquid fermentation medium; and (5) fermenting: performing liquid fermentation and enzyme production by using the newly prepared medium, and determining the optimized enzyme production condition. In the method for producing the laccase, the novel marine endogenous pestalotia is taken as a production strain; and the method has the characteristic that the media are coarse, wide in sources, low in prices and the like.

The invention discloses a marine fungi penicillium crustosum bacterial strain, quinolinone compounds derived from marine fungi penicillium crustosum, and a preparation method and applications of the quinolinone compounds. Specific structural formulas of the quinolinone compounds are represented by formula R1-formula R6. According to the preparation method, a culture medium is inoculated with the marine fungi penicillium crustosum bacterial strain AP2T1 CGMCC No.8516 for static fermentation; an obtained fermentation broth is extracted with ethyl acetate, and mycelium is extracted with an organic solvent; obtained crude extracts are mixed, an obtained mixture is subjected to sephadex column chromatography, reverse-phase silica-gel column chromatography, and thin layer chromatography detection using organic solvents; an obtained elution ingredient containing intermediates is subjected to methylation, and thin layer chromatography and reverse-phase silica-gel column chromatography separation and purification so as to obtain target compounds R1-R6. The target compounds R1-R6 possesses wide application prospect in preparation of antitumor drugs, antibacterial agents, and agricultural insecticides.

The invention relates to a quinones derivative as well as a preparation method of the quinones derivative and an application of the quinones derivative as an antibacterial agent. During the preparation, marine fungi Nigrospora sp.(HM565952) is subjected to strain culture in a culture medium, then, the marine fungi are subjected to fermentation culture in a fermentation culture medium, next, the obtained fermentation liquid is filtered, thalli are removed, and the fermentation liquid is extracted by ethyl acetate; after the extraction liquid is concentrated, chromatographic separation is carried out, and the obtained eluent is concentrated to obtain two red crystals which are respectively 4-Deoxybostrycin and Bostrycin; and acetyl oxide is added into solution in which the two compounds dissolve for reaction to obtain 3-Acetoxy-4-deoxybostrycin and 3-Acetoxy-bostrycin. The quinones derivative provided by the invention has the obvious antibacterial activity on Bacillus subtilis, Bacillus cereus, Micrococcus tetragenus, Micrococcus lutea, Staphylococcus aureus, Staphylococcus albus, Escherichia coli, Vibrio parahaemolyticus and Vibrio anguillarum and can be used for developing antibacterial agents, in addition, raw materials can be produced in large scales, and the resource limitation is avoided, so the application prospect is wide.

The invention relates to a quinolinone alkaloid derivant as well as a preparation method and the application thereof. The preparation method comprises the following steps: firstly, carrying out strain culture for marine fungi in a strain culture medium; then carrying out fermentation culture in a fermentation culture medium; filtering obtained fermentation liquor to obtain thallus; extracting theobtained thallus by an organic solvent; carrying out chromatographic separation for an extracting solution after the extracting solution is concentrated; and concentrating obtained eluent to obtain yellow powder, i.e. the quinolinone alkaloid derivant. The compound has very high killing effect on cotton aphids, diamond back moths and heliothis virescens, can be used as pesticide of microorganismsand also has large-scale production of raw materials and wide application prospect.

The invention provides a penicillazine derivative and the preparation method and the application thereof. The preparation method comprise the following steps: culturing strains of marine fungi in a culture medium, fermentation culturing the fungi in a fermentation culture medium, filtering the obtained fermented solution to remove thalli; heating to concentrate the filtrate and extracting with an organic solvent; carrying out the chromatographic separation and concentrating the eluate to obtain penicillazine; and adding an alkylation or acylation reagent and reacting to obtain the penicillazine derivative. The penicillazine derivative derived from the marine fungihas an enzyme inhibiting activity, can effectively inhibit Alpha-glucosidase, and can be applied for developing enzyme inhibiting pharmaceuticals. The raw material can be produced in a large scale, and the application prospect is wide.

The invention discloses an isocoumarin compound as well as a preparation method and application of the isocoumarin compound as a natural marine organism antifouling agent. The isocoumarin compound is characterized in that the compound has a structure as shown in formula I, wherein R2 and R2 independently represent H and a structure as shown in specification, and R1 or R2 is H; and R3 and R4 independently represent H and OCH3, and R3 or R4 is H. The compound as shown in formula I can be prepared through adopting such means as cultivation, fermentation and chromatographic separation on marine fungi, i.e., Eurotium sp. (HM991283). The natural marine organism antifouling agent disclosed by the invention is efficient and low in toxicity, and is characterized in that the compound of formula I or salt thereof, as an active ingredient, is used for preventing and controlling marine organism fouling caused by barnacle.

The invention belongs to a stain of biological field and relates to marine fungi aspergillus unguis, active extract thereof and a preparation method and the use of the active extract thereof and active components thereof. A stain of marine fungi strain DLEP2008001 separated from Dalian sea area is appraised as aspergillus unguis. After the bacterial stain is fermented in a shake flask, the ethyl acetate extract of the fermentation liquor of the bacterial strain is combined with the alcohol extract of mycelia to form the total coarse extract having methyl penicillin-resistant staphylococcus aureus, pseudomonas aeruginosa and Candida albicans resistance activities; and the extract is subjected to silica gel column chromatography, silica gel thin-layer chromatography and reversed-phase high-efficiency liquid phase separation to obtain two active ingredients, namely a white powder component 1 and a component 2. The extract has remarkable antibacterial activities for the methyl penicillin-resistant staphylococcus aureus, the pseudomonas aeruginosa and the Candida albicans and has potential use as an antibacterial agent.

The invention discloses a marine fungus Trichoderma harzianum DLEN2008005 and application thereof. The marine fungus Trichoderma harzianum DLEN2008005 is collected in China General Microbiological Culture Collection Center (CGMCC) on January 29, 2016, the collection number is CGMCC No.12105, and the collection address is Institute of Microbiology of Chinese Academy of Sciences, Datun Road, Chaoyang District, Beijing City. Meanwhile, active substances in standing fermentation liquid of the marine fungus Trichoderma harzianum DLEN2008005 are extracted and purified, and the strain, a culture of the strain and an extract of the fermentation liquid have very good antioxidant activity and acetylcholin esterase inhibition activity and have very good application prospects in preparation of anti-senile dementia medicines, antioxidant medicines and / or medicines for inhibiting the activity of acetylcholin esterase.

The invention discloses a marine fungus, novel skeleton meroterpenoid derivatives prepared from the marine fungus as well as a preparation method and application of the novel skeleton meroterpenoid derivatives. The marine fungus Aspergillus terreus GZU-31-1 is preserved in Guangdong Microbiological Culture Collection Center on December 17, 2019 and has a preservation number of GDMCC No:60789. Sixnovel skeleton meroterpenoid derivatives are separated from fermented liquid of the marine fungus, have novel structures and are capable of remarkably reducing the activity of triglyceride; particularly, when the concentrations of the compounds 1-5 are 2 microliters and 5 microliters, the content of triglyceride in cells is remarkably reduced; especially the lipid decreasing activity of the compound 1 at a concentration of 5 microliters is even superior to that of a positive control, namely berberine at the same concentration. The compounds provided by the invention have a great application value to lipid decreasing and can be prepared into lipid decreasing drugs for use.

Compounds originally isolated from marine fungi are useful as antifouling (antibacterial and / or anti-larval settlement) agents. The compounds are 3-chloro-2, 5-dihydroxy benzyl alcohol, cyclo-(Pro-Phe),3-methyl-N-(2-phenylethyl) butanamide, and succinic acid. The compounds are non-toxic or low-toxic. They can be used alone or in combination, as active ingredients for making environment-friendly antifouling formulations / coatings.

The invention discloses application of a ring(L-tyrosine-L-proline) in preparation of bacterial quorum sensing system inhibitors. The invention aims to provide a novel bacterial QS inhibitor ring(L-tyrosine-L-proline), the content of the ring(L-tyrosine-L-proline) is relatively high in the metabolites marine fungi, and the ring(L-tyrosine-L-proline) can be extracted on a large scale, or can be obtained by artificial synthesis. Therefore, according to the present invention, the ring(L-tyrosine-L-proline) has the advantages of simple separation method and no drug resistance, and is suitable forlarge-scale preparation.

The invention belongs to the technical field of biological pharmacy and particularly relates to indolizidine alkaloid extracted from marine fungi liquid fermentations, and a preparation method and an application thereof. The indolizidine alkaloid is obtained through separating and purifying marine curvularin IFB-Z10 (curvularia sp.) fermentation liquid. The alkaloid has higher inhibiting effect on acetylcholine esterase and does not have toxicity on cells, so the indolizidine alkaloid can be used as a lead compound of acetylcholine esterase inhibitors, is applied to the acetylcholine esterase inhibitor preparation and can be prepared into medicine for treating Alzheimer's diseases.

The invention belongs to the technical field of pharmaceutical compounds, and particularly discloses a di-sesquiterpene compound derived from marine fungi, a preparation method of the di-sesquiterpenecompound and application of the di-sesquiterpene compound in preparation of anti-inflammatory drugs. The structural formula of the di-sesquiterpene compound is shown in formulas I, II and III, and the di-sesquiterpene compound is prepared by fermenting marine fungus Aspergillus sp. ZJ-68, wherein the marine fungus Aspergillus sp. ZJ-68 was preserved in Guangdong Culture Collection Center on Jan.21, 2019 with the preservation number of GDMCC No: 60541; the production of NO induced by LPS can be significantly inhibited by compounds I, II, and III, specifically, IC50 is 16.48 micrometer, 20.7 micrometer and 24.4 micrometer, respectively, and the IC50 of the positive control indomethacin was 37.5 micrometer. Therefore, the di-sesquiterpene compound can be used for preparing the anti-inflammatory drugs and has the clinical application potential of anti-inflammatory treatment.

A preparation method of a diphenyl ether compound and application of the compound as a natural marine organism antifouling agent. The preparation method is as below: culturing marine fungus Aspergillus sp. (HM535361) in a fungus culture medium; conducting fermentation culture on the fungi in a fermentation medium; then soaking the fermentation products with methanol; conducting decompression concentration to obtain a methanol crude extract; suspending the extract in water; extracting the extract with ethyl acetate, conducting vacuum concentration on the extract and conducting chromatographic separation; and concentrating the eluent to obtain four colorless or light red oil, 4-methoxycarbonyl-diorcinol, 3, 3 '-O-dimethyl-violaceol-I, cordyolC and diorcinol. The invention provides an environment-friendly natural marine organism antifouling agent, which is characterized by using the diphenyl ether compound as an effective ingredient. The antifouling agent can be used for the prevention and treatment of marine organism biofouling caused by attachment of barnacle Balanus Amphitrite larvae, and has the advantages of high efficiency and low toxicity. The raw material can be obtained from mass production by fungal fermentation, and is not subjected to resource constraints; therefore, the invention has wide application prospect.

The invention discloses an extract for marine fungi culture, a preparation method thereof and application thereof. The extract is an oily matter prepared by the following steps: after seed culture and fermentation culture of marine fungi CCTCC M 208095, collection of culture fermentation broth, extraction of the fermentation broth by ethyl acetate and concentration and drying of the residual matter. The extract has the advantages of resisting pathogenic bacteria and oral cancer cells KB and inhibiting acetylcholinesterase, topoisomerase I, and a drug-resistant strain KBv200 of the extract, and can be used for preparing antibacterial and antineoplastic medicines for treating senile dementia.

The invention discloses a compound lithocarolsA-F, a preparation method and application thereof in preparation of antitumor drugs. The compound lithocarolsA-F is isolated from a fermentation culture of marine fungi Phomopsis lithocarpus FS508. Experiments prove that the IC50 values of the compound lithocarolsA-F on liver cancer cells HepG-2, breast cancer cells MCF-7, glioma cells SF-268 and non-small cell lung cancer cells A549 are 10.5-87.7 muM, showing that the compound lithocarolsA-F has relatively significant antitumor activity and can be used for preparing antitumor drugs.

The invention relates to a marine fungi secondary metabolite derivative and an application of the marine fungi secondary metabolite derivative as a marine biological antifoulant. The derivative has the structure represented by a formula I as shown in the specification, wherein R1 and R2 are independently H, a group as shown in the specification, a group as shown in the specification or a group as shown in the specification and one of R1 and R2 is H; R3 and R4 are independently H and OCH3 and one of R3 and R4 is H; R5 and R6 are independently H, C1-C4 alkyl, C2-C4 alkylacyl, C1-C4 haloalkyl, C1-C4 alkylsulfonyl, C2-C4 alkenyl, C3-C8 cycloalkyl and C7-C12 arylalkyl, wherein alkyl, alkylacyl, haloalkyl, alkylsulfonyl, alkenyl, cycloalkyl and arylalkyl are optionally substituted with one or more hydroxy, mercapto, amino, halo, C1-C4 alkyl groups; '-----' represents a single bond or absence; and the prerequisite is that at least one of R5 and R6 is not H when '-----' represents a single bond.

The invention discloses a marine fungi penicillium thomii, an extract and application thereof. A marine fungi penicillium thomii (penicillium thomii) FS77 was preserved at China Center for Type Culture Collection (CCTCC) at Wuhan University of Wuhan City of China on November 24th, 2011 and the preserving number is CCTCC M201414. The fermentation extract has restraining effects on staphyloccocus aureus, bacillus subtilis and pseu domonas aeruginosa. Simultaneously, the fermentation extract has resisting effects on plant pathogen fungi alternaria alternate, colletotrichum gloeosporioides and cylindrocladium scoparium. Accordingly, the marine fungi penicillium thomii provides selectable chemicals for studying and developing new antibacterial chemicals and biopesticides, and provides scientific evidences for developing natural active materials of a marine fungi source.

The invention discloses mutagenized Aspergillus unguis 6-20-6, collected in Guangdong Microbial Culture Collection Center on May, 20, 2018 under GDMCC 60377; the collection address is Guangdong Institute of Microbiology, Fifth floor, No. 59 building, No. 100 Compound, Central Xianlie Road, Guangzhou. The mutagenized Aspergillus unguis 6-20-6 provided herein can generate active materials, compoundsA and B, having antibacterial, antifungal, insecticidal or acetylcholinesterase-inhibiting effects; the structural formulas of the compounds A and B are shown as formula 1 and formula 2 respectively;the mutagenized Aspergillus unguis 6-20-6 has a promising application prospect in the preparation of antifungal agents, antibacterial agents, agricultural insecticides or acetylcholinesterase inhibitors.

The invention provides a marine fungi-derived azaphilones compound, a preparation method thereof and application thereof in preparing a medicine for preventing agricultural pathogenic fungi, the marine fungi is Pleosporales sp. HBU-135 strain, the preservation date is October 18th, 2018, and the preservation number is CGMCC No. 16379, the preservation unit is the General Microbiology Center of theChina Microbial Culture Collection Management Committee, and the preservation address is No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing. The structural formula of the compound is as shownin a formula 1 or a formula 2. The Pleosporales sp. HBU-135 strain is cultured by fermentation, and the above compound is isolated from the fermentation product. Through the verification, the compound has strong inhibitory activity against various agricultural pathogenic fungi, can be prepared into an antibacterial agent, and has broad application prospects.

The invention discloses an application of a quinazolinone alkaloid compound derived from marine fungi in the prevention and control of marine fouling organisms. The application of the quinazolinone alkaloid compound in the prevention and control of marine fouling organisms is disclosed for the first time, and the quinazolinone alkaloid compound has a significant inhibitory effect on the adhesion of marine fouling organisms on solids, and has a significant effect under low content coating. At the same time, when inhibiting the attachment of marine organisms, it will not kill marine organisms, but will play a repellent role. The compound can not only be extracted from the fermentation products of marine fungi, but also be obtained through artificial mature synthesis process, has reliable andstable sources, and has good application prospects in the prevention and control of fouling organisms in various marine artificial facilities.

The invention provides anthraquinone compounds, a preparation method and an application, and belongs to the field of compounds. The anthraquinone compounds have the activity of resisting pathogenic bacteria of plants, and can be used for preventing and treating plant diseases caused by the pathogenic bacteria of the plants when applied as bactericides. The anthraquinone compounds are extracted from marine fungus Fusarium equiseti GLY27 with the collection number being CGMCC (China General Microbiological Culture Collection Center) 14348. The anthraquinone compounds are used for preventing and treating the plant diseases caused by the pathogenic bacteria of the plants, wherein the pathogenic bacteria of the plants comprise P.syringae, A.avenae and E.carotovora.