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Industrial use of marine fungus fission chytrid OUC88

A Schizochytrium and marine technology, applied in fungi and other directions, can solve the problems of low DHA production, and achieve the effect of increasing DHA content and increasing cell biomass

Inactive Publication Date: 2006-07-19
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to address the defects of the prior art: the problem of low DHA yield in the screening culture of this bacterium. It is planned to start from the original strain and through artificial mutagenesis to select and breed a schizopot with a higher content of DHA. new varieties of bacteria; and the factors affecting the growth of Schizochytrium and the growth characteristics of the bacteria were studied, further improved on the basis of traditional culture techniques, and a set of Schizochytrium with simple preparation process and high DHA content was established. Cultivation process for the development of new species of Schizochytrium for industrial application

Method used

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  • Industrial use of marine fungus fission chytrid OUC88
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: Mutation selection of Schizochytrium:

[0026] Pick a single colony of Schizochytrium SR21 and place it in a conical shaker flask containing 50ml of liquid medium (glucose 30g / l, yeast extract 10g / l, 50% natural seawater), and cultivate it with shaking at 180rpm at 25°C Two days later, add 0.05 mol / mL ethyl methanesulfonate (EMS) under aseptic conditions to make the final concentration 0.08 mol / L, shake continuously for 30 minutes, centrifuge the culture solution from the previous step under aseptic conditions and Wash the bacteria, that is, after washing 5 times with sterile water, centrifuge the washed bacteria solution to make the OD of the bacteria solution 650 After reaching 0.3---0.8, transfer it to a petri dish to make the centrifuged and washed bacterial liquid into a thin layer of bacterial liquid with a thickness of 1-5 mm; then mutagenize 0 (control) at a distance of 30 cm under a 20 W ultraviolet lamp. , 10, 20, 30, 40, 50, 60 seconds, then ce...

specific Embodiment 2

[0032] Specific embodiment 2: the impact of carbon, nitrogen source concentration and carbon-nitrogen ratio on the biomass of Schizochytrium OUC88 and DHA content:

[0033] In a 250ml Erlenmeyer shaker flask, add 50ml of natural seawater (taken from the sea area of ​​Qingdao) and 50ml of distilled water respectively, add carbon source (glucose) and nitrogen source (yeast extract) according to the concentrations shown in Table 1, and adjust the pH value to 6.0 , after autoclaving, insert 5ml of pre-cultivated OUC88 strain seed solution, and vibrate and culture on an air bath shaker at 25° C. for 5 days, and the shaking speed is 180 rpm. The cells were collected by centrifugation, freeze-dried to constant weight at -50°C, and the dry weight was measured; 3 -methyl esterification by methanol method, as described in Example 1, the percentage composition of DHA in the thalline was determined by gas chromatography. The results are shown in Table 2.

[0034] instance numbe...

Embodiment 3

[0037] Example 3: Determination of the growth status and DHA accumulation status of Schizochytrium OUC88 in different periods:

[0038] In a 150ml Erlenmeyer shaker flask, add 25ml of natural seawater (taken from the sea area of ​​Qingdao) and 25ml of distilled water respectively, then add glucose with a concentration of 60g / L and yeast extract of 30g / L to adjust the pH value to 6.0, and do 7 A parallel sample, after autoclaving, was inserted into 1ml of pre-cultivated seed solution, and was shaken and cultivated on an air bath shaker at 25°C, with a shaking speed of 180rpm, and a bottle of sample was taken out for analysis every 22 hours, in order to observe fission Chytrium growth and DHA accumulation. Bacteria were collected by centrifugation and freeze-dried to measure their dry weight; the content of DHA in them was determined by the method described in Example 1. see results figure 1 .

[0039] From figure 1 As can be seen in , Schizochytrium cell proliferation and D...

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Abstract

The present invention relates to the industrial application of ocean fission chytrid OUC88. The ocean fission chytrid OUC88 has capacity of generating rich docosahexaenoic acid (DHA), and has the preservation number of CGMCC No. 1240. The ocean fission chytrid OUC88 is new strain obtain with fission chytrid SR21 as originating strain and through chemical induction mutation and physical induction mutation. Through optimizing culture condition and replenishing carbon source in later culture stage, the present invention has dry cell weight reaching 25 g / L and DHA product up to 8.27 g / L. The thallus may be used as the additive for bread, mild product and other food products, especially baby's milk powder and can promote body's growth. The present invention has simple thallus fermenting process, high bioavailability, high yield of destination product and low cost.

Description

technical field [0001] The invention relates to the improvement of marine microorganism application technology, in particular to the industrial application of a marine fungus Schizochytrium OUC88. It belongs to the technical field of microbial application. Background technique [0002] In recent years, the important role of polyunsaturated fatty acids (PUFAs) on human health has been paid more and more attention. Among them, as one of the important ω-3 PUFAs, docosahexaene (hereinafter referred to as: DHA) [docosahexaenoic acid (22:6, n-3)] has received much attention because of its important physiological functions in humans and animals. People's favor. Studies have shown that DHA is the basic component of cell membranes in some tissues of the human body; it plays an important role in the development of the baby's visual system and nervous system; it also has pharmacological effects such as lowering cholesterol, anticoagulation, and preventing cancer; An essential fatty ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P7/64
Inventor 张学成朱路英宋晓金况成宏
Owner OCEAN UNIV OF CHINA
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