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183 results about "Infants milk" patented technology

Analyzing method of sialic acid in infant milk powders with ultra-high performance liquid chromatography tandem quadrupole mass spectrometry

The ineveniton discloses an analyzing method of sialic acid in infant milk powders with an ultra-high performance liquid chromatography tandem quadrupole mass spectrometry method. On the basis of an original detecting of the sialic acid in milk powders with high performance liquid chromatography, the ultra-high performance liquid chromatography (UPLC) technology is combined, the sialic acid in three forms in a sample is extracted, and therefore the method of detecting the sialic acid in infant formula milk with the UPLC tandem mass spectrometry, wherein the method is higher in sensitivity and faster. According to the method, the average recovery of the sialic acid is within 84.3%-98.9%, the relative standard deviation (RSD) is 4.9%-8.2%, and the lowest detection limit of the sialic acid is 0.01 microgram/milliliter. The analyzing method of the sialic acid in infant milk powders with the UPLC tandem quadrupole mass spectrometry is simple and convenient to operate, a better separation mode is adopted, matrix effects are relatively small, the process of high-speed centrifugation after hydrolysis is adopted in pretreatment and simple, easy and convenient to operate, the loss of the sialic acid in a pretreatment process is effectively avoided, and the recovery rate is improved.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY
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