100 results about "Protein digestibility" patented technology

The invention provides an aquatic feed which is prepared from soybean waste, steam heated fish meal, fish meal, brewer's yeast, squid meal, shrimp shell powder, choline chloride, flour, calcium dihydrogenphosphate, scallop powder, Ulva lacbuca Linnaeus powder, glutelin powder, phosphatidy icholine, fish oil, palmitic oil, maltose dextrin, amino acid fish grout, vitamins for shrimps, microelements, immunological polysaccharides, copper-methionine and complex enzyme. The invention also discloses the pre-maturing process for the aquatic feed.

Nutritive proteins are provided. In some embodiments the nutritive proteins comprise a protein digestibility corrected amino acid score (PDCAAS) that exceeds a benchmark protein. Also provided are nucleic acids encoding the proteins, recombinant microorganisms that make the proteins, methods of making the proteins using recombinant microorganisms, compositions that comprise the proteins, and methods of using the proteins, among other things.

The invention discloses an alfalfa half-dry bagged silage method which can be used for solving the problem in harvesting alfalfa in rainy season. The alfalfa half-dry bagged silage method comprises the following steps of: harvesting, airing, chopping, packing, compacting, sealing, fermentation and the like. Through the silage regulation in the invention, relatively many nutrient substances can be stored, the texture is soft, the source and sweet fresh scent is realized, and the palatability is improved; and meanwhile, the alfalfa silage is higher than the hay product in terms of energy and protein digestibility.

The invention discloses a preparation method of a feather fermentation protein feed. The preparation method is characterized by comprising the following steps of adding each kilo of feather into 20 to 30l of inorganic salt culture medium for mixing, sterilizing at the temperature of 115 to 121DEG C for 15 to 20 minutes, and cooling to room temperature; inoculating a bacillus licheniformis seed solution into the inorganic salt culture medium in which the feather is added according to the volume ratio of 3 to 5 percent; introducing 0.15 to 0.18 cubic meter of sterile air into each cubic meter of solution in each minute for ventilating and fermenting at the fermentation temperature of 30 to 37DEG C until the feather is completely dissolved; performing filtering and impurity removal on fermentation liquor, then adding maize flour with the mass percent of 6 to 7 percent and bran with the mass percent of 4 to 5 percent into filtrate, uniformly mixing, drying and granulating to obtain the feather fermentation protein feed. According to the method disclosed by the invention, the content of soluble protein in the fermentation liquor reaches 31.4 to 34.3g / L; for the fermentation protein feed prepared from the maize flour, the bran and flour serving as auxiliary materials, the mass percent of crude protein reaches 28.6 to 29.5 percent, and the protein digestibility in vitro reaches 89.5 to 92.4 percent. The protein feed prepared by the method disclosed by the invention has natural maize and wheat fragrance, is good in palatability, and is conductive to digesting and promoting the growth.

The present invention provides a process for producing a fertilizer composition from still bottoms obtained from milling of a starch-containing feedstock (such as corn), comprising separating still bottoms into a solids stream and a thin stillage stream; removing water to generate a condensed distillers solubles stream; and recovering the condensed distillers solubles stream as a fertilizer composition. Novel fertilizer compositions as well as novel distillers grains products are co-produced with a fermentation product (such as ethanol or isobutanol). Benefits include the ability to recycle valuable crop nutrients in a sustainable manner; improved ethanol life cycle analysis due to lower crop inputs and lower energy consumption; reduced environmental burden from industrial animal farming due to nitrogen and phosphorous emissions; higher consumption of distillers grains in ruminants currently limited by sulfur and / or fat inhibition; and higher consumption of distillers grains in monogastrics due to higher protein digestibility and less heat damage.

Disclosed is a method for improving the digestibility and amino acid profile of proteinaceous animal feed products by the use of a combination of acidic, alkaline and / or neutral proteases. The selection and amounts of the proteases is based on the particular animal feed product and on the conditions of the gastrointestinal tract of the animal to be fed the treated animal feed. Use of the treated animal feed will reduce the amount of amino acid supplementation required, reduce the amount of nitrogen in animal feces, and improve the economical performance of the animal.

The invention relates to an optimal enzyme preparation application system (OEATM). The system comprises effective enzyme activity evaluation and effective nutrient value evaluation, wherein the effective enzyme activity evaluation comprises six indexes, namely labeled enzyme activity, a pH threshold value, excess gastric acid measurement, excess protease measurement, high-temperature resistance measurement and a three-month enzyme activity retention rate; and the effective nutrient value evaluation comprises four indexes, namely feed viscosity measurement, feed energy value measurement, feed protein digestibility measurement and animal growth performance. By a new idea and a new method, the problems in the application process of a feed enzyme preparation are solved; and experiments prove that the OEATM has higher pertinence to a complex enzyme and lower cost.

The invention provides noodles capable of reducing a blood sugar index. The noodles comprise effective nutrient components of whole grains. Besides, the whole grains which are rich in components for reducing blood sugar are compounded, so that after the noodles are eaten, the blood sugar index of a human body cannot be greatly increased. In addition, the invention further provides a making method of the noodles capable of reducing the blood sugar index. Through enzymolysis and super-fine comminution, the mouth feel of the noodles can be improved, the digestion and the absorption of beneficial nutrition are promoted, and besides, anti-nutritional factors in the whole grains can be eliminated; through an ageing technology, the content of resistant starch is increased. The hardness, elasticity and cohesiveness of the noodles capable of reducing the blood sugar index, provided by the invention, are equivalent to those of general fine dried noodles sold in markets, and the protein digestibility and the content of the resistant starch of the noodles disclosed by the invention are higher than those of the general fine dried noodles sold in markets.

The present invention provides methods for continuous measurement of protein and / or triglyceride digestibility during a meal. Stable isotope of 15N-spirulina protein and 2H5-phenylalanine was added to the nutritional supplement. Protein digestibility is calculated by measuring the ratio [15N]PHE to [2H5]PHE in plasma and the nutrition. In another embodiment, [1,1,1-13C3]tripalmitin and [2,2-2H2]palmitic acid were added to the meal. The ratio between [1-13C]palmitic acid / [2,2-2H2]palmitic acid will represent the percentage digestion of triglycerides by lipase from the pancreas.

The invention relates to the technical field of pet food and particularly relates to gluten-free cat food and a preparation method thereof. The cat food is mainly prepared from the following raw materials in parts by weight: 10 to 20 parts of potato, 10 to 15 parts of pea protein powder, 2 to 3 parts of pumpkin, 3 to 5 parts of spiral seaweed, 3 to 4 parts of spinach, 4 to 6 parts of leaf mustard,4 to 6 parts of carrot, 25 to 35 parts of beef, 15 to 25 parts of chicken, 5 to 10 parts of mussel meat, 1 to 2 parts of fructo-oligosaccharide powder, 5 to 10 parts of olive oil, 1 to 3 parts of lactobacillus paracasei and 1 to 5 parts of table salt. The pea protein powder, the potato, the pumpkin and the like added into the gluten-free cat food disclosed by the invention are hypoallergenic rawmaterials without cereal protein; the purpose of preventing allergy can be achieved by reducing cereals in the raw materials. The fructo-oligosaccharide added into the gluten-free cat food disclosed by the invention can achieve the purpose of improving the protein digestibility.

The invention discloses a whole grain premixed dumpling flour containing puffed sorghum and a preparation method thereof. According to the whole grain premixed dumpling flour, wheat flour and whole grain red sorghum puffed powder are taken as total flour materials, wherein weight ratio of wheat flour to the whole grain red sorghum puffed powder ranges from 5:5 to 7:3; and the whole grain premixed dumpling flour is prepared via adding vital gluten, soy isolate protein, and konjac glucomannan for mixing at an appropriate ratio. According to the preparation method, puffing technology is adopted for processing whole grain sorghum powder, so that problems caused by application of whole grain sorghum powder in dumpling wrapper processing are solved, wherein the problems are that: adding ratio is low, molding is difficult to realize, product palatability is poor, and protein digestibility is low. The obtained whole grain premixed dumpling flour possesses unique color and scorch aroma flavor of sorghum puffed powder; mouthfeel of dumpling wrappers prepared from the whole grain premixed dumpling flour is smooth; technology demands of hand made or machine made dumpling wrappers are satisfied; dumpling kinds are increased; application method is simple; and the whole grain premixed dumpling flour is suitable for household dumpling preparation, restaurant fancy cooking, and coarse cereal dumpling production of processing enterprises.

The invention belongs to the technical field of food processing, and in particular relates to a preparation method of soy sauce brewed by extruding expanded soybean meal and flour. The preparation method of the soy sauce brewed by extruding the expanded soybean meal and the flour comprises the following steps: (1) mixing the expanded soybean meal and the flour for extruding; (2) adding bran and mixing, and moistening a mixture of the bran and extrudate of expanded soybean meal and the flour with water; (3) according to the conventional soy sauce preparation method, mixing soybean pieces with the flour, inoculating, carrying out starter propagation, and fermenting to obtain the soy sauce; (4) sterilizing and filtering the obtained soy sauce, and then packaging to obtain the finished product. According to the preparation method, the raw materials such as the soybean meal and the flour, which are used for producing the soy sauce, are pretreated by adopting a extrusion and swelling technique, so that the aims of moderately modifying proteins, gelatinizing starches and passivating anti-nutritional factors as well as sterilizing can be achieved; compared with the traditional cooking method for treating the raw materials, the preparation method provided by the invention can increase protein digestibility and amino acid production rate.

The invention provides an in-vitro method for comprehensive assessment of protein biological quality in infant formula milk powder. The infant milk powder is firstly re-dissolved to detect total proteins and total acid hydrolyzed amino acids, then the infant milk powder is digested by artificial simulated gastric juice and intestinal juice and precipitated by trichloroacetic acid with the concentration of 10 percent, and soluble nitrogen and amino acids in supernatant liquor are detected. The protein digestibility and the essential amino acid digestibility are calculated through the detection results, the modified amino acid score is obtained by multiplying the total amount of essential amino acids in the supernatant liquor and a model contrast value which is recommended by WHO by the essential amino acid digestibility, and the protein biological quality is determined by the value which is obtained by multiplying the protein digestibility by the modified amino acid score.

The invention provides a cold oil pressing method. The method comprises the following steps: (1) cleaning raw oil; (2) husking the raw oil to separate husks from kernels; (3) performing flaking to obtain a material blank; (4) softening the material blank; (5) performing cold pressing on the material blank; (6) precipitating the material blank, filtering oil, and collecting a leach liquor to obtain crude oil; and collecting a precipitate to obtain a filter cake; (7) adjusting the acid value of the crude oil till the pH value is less than or equal to 3; (8) centrifuging the crude oil obtained in the step (7), and stopping centrifuging after an index is reached; and (9) deodorizing the centrifuged crude oil, and performing inspection to obtain a refined oil product after the inspection is passed. The refined oil product prepared by using the cold oil pressing method provided by the invention can well keep nutrient substances in the raw oil, the oil and the oil cake do not change color, and meanwhile energy is saved; obtained protein meal is golden yellow; and compared with the conventional process, the cold oil pressing method has the advantages that the protein digestibility of low-temperature cottonseed meal is increased greatly, the gossypol content is lowered greatly, and damages to animal health are reduced while nutrients are increased.

The invention discloses a method for synergistic fermentation of soybean meal by employing bacillus subtilis and neutral protease. The hydrolysis efficiency of the soybean meal is improved; the stability of a product is well improved; the optimal process is optimized; the inoculation amount is 2.7%; the adding amount of the neutral protease is 304U / g; the material-water ratio is 1 to 1.7; the treatment temperature is 45 DEG C; the treatment time is 47 hours; the content of peptide in the soybean meal which is subjected to the synergistic fermentation by the bacillus subtilis and the neutral protease is increased to 182.15mg / g from 13.35mg / g; the content of crude protein is increased to 56.82% from 47.12%; the in-vitro protein digestibility is increased to 93.19% from 82.46%; the in vitro attrition rate of dry matters is increased to 72.33% from 66.11%; meanwhile, antigen protein and macromolecular protein in the soybean meal of a fermented material are effectively degraded; anti-nutritional factors in the soybean meal can be reduced by the synergistic treatment of the bacillus subtilis and the neutral protease; the nutrient value of the soybean meal can also be improved; and a foundation is laid for production of the high-quality forage soybean meal.

The invention discloses a large yellow croaker feed additive with an intestinal tract protection effect and a preparation method and application of the large yellow croaker feed additive. The large yellow croaker feed additive is characterized by consisting of the following raw materials in parts by weight: 18-25 parts of rosemary oil, 16-26 parts of peppermint oil, 15-20 parts of citrus oil, 20-30 parts of calcium alginate, 10-20 parts of arginine and 5-10 parts of glutamine. The preparation method comprises the following steps: uniformly mixing the rosemary oil, peppermint oil, citrus oil, calcium alginate, arginine and glutamine according to the weight parts, and performing spray drying. The large yellow croaker feed additive has the advantages that the addition mass of the large yellow croaker feed additive in the feed is only 0.07-0.12 percent, villus in the intestinal tract of the large yellow croaker can be prevented from being damaged by antinutritional factors in a plant protein source, growth of the large yellow croaker is obviously promoted, the weight gain rate can be improved by 8 percent, the daily ration is improved by over 10 percent, and the protein digestibility is improved by 13 percent.

A protein beverage containing protein and water is prepared from the proteins of plant and animal through defatting, mixing, adding deionized water, adding composite proteinose, enzymolyzing, filtering, separating to obtain refined liquid, concentrating, adding deionized water, sterilizing, and filling it in containers. It is rich in balanced multiple kinds or protein and 9 amino acids.

The present disclosure relates, according to some embodiments, to methods and systems for processing a high-concentration protein product from a microcrop (e.g., aquatic species, Lemna) and compositions thereof. According to some embodiments, the present disclosure relates to a method of processing a biomass comprising a microcrop (e.g., Lemna), where the method may include: blanching a first portion of the biomass in a blanching solution to form a wet protein concentrate; separating the first wet protein concentrate from a separated solution (e.g., using a screw press, using a vibratory screen); and drying the first wet protein concentrate to form at least one of a first protein concentrate flake and a first protein concentrate granule. In some embodiments at least one of the first protein concentrate flake and the first protein concentrate granule may comprise at least 45% protein and a Protein Digestibility Corrected Amino Acid Score (PDCASS) value of at least 0.88. In some embodiments, the present disclosure relates to protein products and compositions derived from a microcrop (e.g., derived from Lemna).

The present invention provides high-collagen protein and high-calcium noodles. The noodles have high collagen protein and calcium content, and have efficacy of beautifying, health care, and preventing osteoporosis. In addition, the invention provides a preparation method of the noodles. Through Crushing and ultra-micronizing process, bones are finely micronized, so that the content of free calcium and the absorption rate of calcium are increased. Meanwhile, the elasticity and toughness of the noodles are improved, and mouthfeel is more chewable. Decocting process can promote the dissolution of bone nutrients, improve the nutritional value of the product, and provide thick aroma and flavor. The noodles provided by the invention have slightly better mouthfeel, elasticity and toughness than commercially available ordinary noodles. The calcium content, collagen protein content, protein digestibility and starch digestibility is higher than ordinary noodles. The mouthfeel is better.

The invention relates to a preparation method of aquatic plant fodder, which is characterized in that hyacinth and / or alligator weed are adopted as the material; the powder is produced after pressing dry, fermenting, drying and grinding; then 50% to 70% of the obtained powder by weight is taken with 30% to 50% of bran residue by weight added in and mixed to prepare the coarse fodder; and the flavoring agent with the weight equivalent to 0.5% to 1% of that of the coarse fodder is added in and mixed with the obtained coarse fodder to prepare the concentrated fodder. The preparation method and the prepared aquatic plant fodder have the advantages that protein digestibility in the fodder can be improved by 13% to 17%; milk yield, milk quality and fleshy fresh flavor of pigs, cows and sheep can be improved; feeding attraction quantity and palatable taste of pigs, cows and sheep can be improved; production, sale and use can be conducted according to three different ways of powder, coarse fodder and concentrated fodder; the problems brought to the environment and shipping by the aquatic plants such as hyacinth and alligator weed can be solved; and the fodder has great economic and social benefits.

The invention discloses an environment-friendly production process of high-protein feeding meat meal, comprising the following steps of: (1) removing impurities and cleaning leftovers generated after animal products are processed; (2) grinding and cutting so as to crush the leftovers; (3) entering into a hot spraying furnace and then carrying out three-stage heating and pressurizing, wherein the pressure is 0.5-1kg, the first-stage temperature is 100-110 DEG C, the second-stage temperature is 120-140 DEG C, and the third-stage temperature is 150-160 DEG C; recovering water steam by condensation and deodorizing and discharging non-condensable gas; and (4) entering into a squeezer to carry out solid-liquid separation, wherein solid phase: cooling, drying, crushing and sieving meat meal, then inspecting and finally packaging and putting in storage, and liquid phase: inspecting oil and then packaging and putting in storage. The invention has the advantages that: 1. by adopting a hot spraying technology, defatting and dewatering are carried out by the screw type squeezer through three-stage heating and pressurizing at the temperature of 100-160 DEG C to synchronously carry out water evaporation, fat collection and meat meal preparation, thereby not only improving the protein digestive rate of the meat meal but also increasing the dry substance content and the protein content in the meat meal; 2. the water content and the fat content are low, and the shelf life is long; 3. the palatability of the meat meal is improved, and the grazing rate of animals is increased for more than 90 percent from 50 percent; and 4. energy saving and environmental-protection discharge are realized by water steam recovery and discharged gas purification.

The invention provides nutrient milk powder. Each 1,000kg of the nutrient milk powder comprises the following components: 18-32kg of desalted whey powder, 312-393kg of alpha-lactalbumin, 12-31kg of concentrated lactalbumin, 291-359kg of cream, 22-44kg of n-3 polyunsaturated fatty acid and 132-201kg of n-6 polyunsaturated fatty acid, wherein the mass content ratio of the n-6 polyunsaturated fatty acid and the n-3 polyunsaturated fatty acid is (3:1)-(6:1). The nutrient milk powder ensures the demanded amount of comprehensive breakfast nutrition, and meanwhile the amino acid composition is conditioned and optimized, so that the PDCAAS (Protein Digestibility Corrected Amino Acids Score) of a product is equal to 1, the product is natural and safe, the raw materials are strictly selected, no food additives such as flavors and fragrances are added, and the nutrition components completely meet the recommended amount of food instruction of China. The nutrient milk powder is taken together with staple food, and energy, protein, carbohydrate and fat all meet the requirements on nutrition of breakfast.

The invention discloses probiotic fermented germinated acid soybean biscuits and a production process thereof. The probiotic fermented germinated acid soybean biscuits consist of the following components in part by weight: 1.5 to 3 parts of germinated acid soybean dregs, 2.5 to 5 parts of flour, 1 to 1.5 parts of sugar, 1.5 to 3 parts of fermentation liquor, 1 to 1.5 parts of water, 0.5 to 1 partof edible oil, 0.25 to 0.5 part of egg liquid, 0.2 to 0.5 part of milk powder, and 0.05 to 0.1 part of baking soda. The production process comprises the following steps of: 1, soaking for germination; 2, fermenting germinated soybean; 3, obtaining germinated acid soybean dregs and fermentation liquor; 4, blending for the germinated acid soybean biscuits; and 5, molding and baking. By the invention, the content of toxic, harmful or anti-nutrient substances in the soybean can be reduced or removed, the protein digestibility is improved, the content of nutrients such as limiting amino acid and vitamins in the soybean is improved, various amino acids are generated through probiotic fermentation, the digestibility, absorption and nutritional value of the probiotic fermented germinated acid soybean biscuits can be improved, and the probiotic fermented germinated acid soybean biscuits have the effects of regulating the intestines, resisting bacteria, preventing diseases, improving immunity and cancer resistance, and slowing cell aging to prolong life, and have moderate sour and sweet taste, rich bean flavor and mellow fermentation aroma.

The invention provides a high anthocyanin noodle, which takes purple sweet potato as the main raw material, can supplement anthocyanin, cellulose, amino acids, vitamins and other nutrients. In addition, the invention provides a making method of the high anthocyanin noodle. By means of acidic alcohol solution extraction, high concentration anthocyanin powder can be obtained, and the anthocyanin content of the noodle is also greatly improved; by means of super-fine comminution, the dissolution or degradation surface area of nutrients can be increased, dissolution of anthocyanin and dietary fiber, protein digestibility and starch digestibility can be greatly improved; and through high temperature standing after dough kneading, partial gelatinization of starch can be promoted, viscoelasticity of the dough is increased, noodle breaking during noodle rolling and forming can be reduced, and soup turbidity of the product is also reduced. The noodle provided by the invention has taste and other mechanical properties similar to those of commercially available ordinary fine dried noodles, and has anthocyanin content, water soluble dietary fiber content, protein and starch digestive performance superior to those of ordinary fine dried noodles.

The invention provides high-calcium noodles and a making method thereof. Natural high-calcium food materials are used as raw auxiliary materials of the noodles, which can complement calcium sources and high quality protein, so that the high-calcium noodles have the functions of preventing hyperosteogeny, improving physique and the like. Through an ultra tiny and nutrient compounding technique, the making method provided by the invention can grind skeletons into granules under 5 microns, enlarges the dissolving surface area or the degrading surface area of nutrients, is favorable for absorption, is good in mouth feel, and greatly improves the protein digestibility and the starch digestibility. The noodles provided by the invention have the similar mouth feel and the similar mechanical property as those of general sold fine dried noodles, the content of the calcium and the content of collagen in the high-calcium noodles disclosed by the invention are higher than those of the general fine dried noodles, and protein digestibility and the starch digestibility are better than those of the general fine dried noodles.

The invention relates to the technical field of enzyme application, and in particular to a method for degrading alcohol soluble protein. The method comprises the step of degrading alcohol soluble protein by using keratinase. The method to prepare the low-alcohol soluble protein feed has excellent effect, can well degrade the alcohol soluble protein, and reaches protein digestibility of 90% or more, and alcohol soluble protein digestibility of 80% or more. The method fully utilizes indigestible alcohol soluble protein in the alcohol soluble protein feed, improves the feed protein digestibility,increases economic benefits of aquaculture, reduces the nitrogen content of livestock and poultry excrement, and avoids environmental nitrogen pollution caused by emission.

The invention discloses a processing method for improving use efficiency of soybean meal and / or rapeseed meal and / or cottonseed meal in a digestion and absorption process of ruminants. The processing method comprises a processing technology, required materials and technological parameters. Through a series of physical and chemical reactions, saccharified soybean meal and / or rapeseed meal and / or cottonseed meal can reach abomasums through rumens of the ruminants, and the protein digestibility of the saccharified soybean meal and / or rapeseed meal and / or cottonseed meal in a whole digestive system of the ruminants is improved, accordingly, protein resources are saved, and are converted into animal products to the greatest extent, the culture benefit of the ruminants is further improved, and remarkable social benefits and economic benefits are achieved.

The invention relates to the fields of agricultural planting and aquaculture and discloses an oryzias latipes cultivation method. According to the oryzias latipes cultivation method disclosed by the invention, carp and crucian varieties of oryzias latipes is cultivated in a rice field planting water system; as a lot of nutrient substances and physiological active substances contained in rice flower are absorbed, the oryzias latipes with high nutrient content is obtained; especially the protein content of the oryzias latipes is 4 to 5 times of that of ordinary fish. The oryzias latipes is delicate in meat, fragrant in flavor and especially easy to digest by a human body, and the protein digestibility can reach 98%. As no any pesticide is utilized in a cultivation process of the oryzias latipes cultivation method, the oryzias latipes and rice have no any chemical pollution; thus, the oryzias latipes cultivation method has extremely high market prospect, utilized cultivation fodder can serve as excellent nutrients of the rice after being absorbed and utilized by the oryzias latipes, rice yield is improved, and the oryzias latipes cultivation method is an extremely scientific and economic cultivation mode.

The invention discloses a fermentation method for improving water-soluble proteins of soybean meal. The fermentation method includes the following steps: separately carrying out liquid fermentation culture on Saccharomyces cerevisiae and Bacillus subtilis with a fermentation tank, and then mixing fermentation culture medium of the Saccharomyces cerevisiae and the Bacillus subtilis to form probiotic fermentation broth; mixing the probiotic fermentation broth, neutral protease, water and soybean meal to obtain a fermentation mixture, carrying out initial fermentation at 30 DEG C for 24 hours, heating the fermentation mixture to 45 DEG C, carrying out additional fermentation for 48 hours, and carrying out low temperature drying, thereby obtaining fermented soybean meal. The fermented soybeanmeal obtained by the fermentation method has high contents of water soluble proteins, and the goal of improving protein digestibility is achieved.