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Method for degrading alcohol soluble protein

A technology of gliadin and keratinase, which is applied in the field of enzyme application, can solve problems affecting the economic benefits of breeding, reduce the digestibility of feed protein, and environmental nitrogen pollution, and achieve the goal of improving economic benefits of breeding, reducing nitrogen content, and high economic value Effect

Inactive Publication Date: 2019-03-19
JINANBESTZYME BIO ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Corn, wheat and other high prolamin raw materials are widely used in the feed industry. If the indigestible high prolamin components cannot be fully utilized, the digestibility of feed protein will be greatly reduced, which will affect the economic benefits of breeding. High nitrogen content, discharge into the environment will also cause environmental nitrogen pollution

Method used

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  • Method for degrading alcohol soluble protein
  • Method for degrading alcohol soluble protein
  • Method for degrading alcohol soluble protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The preparation of bacillus licheniformis producing keratinase comprises the following steps:

[0023] (1) Strain and culture medium preparation

[0024] Strains: keratinase strain Bacillus licheniformis, purchased from China Guangdong Microbial Culture Collection Center, the strain number is GIM1.11;

[0025] Media include slant broth media, expansion media, seed media and fermentation media;

[0026] Slope culture: broth medium composition: 0.8-1.2% beef extract, 0.8-1.2% peptone, 0.5% sodium chloride, 1.8-2.2% agar, pH7.2-7.5, sterilized at 118-122°C for about 30-40min ;

[0027] Expansion medium: 0.5% sodium chloride, 0.4%-0.6% yeast extract, 0.8%-1.2% beef extract, sterilized at 118-122°C for about 30-40min;

[0028] Primary seed medium: peptone 1.8%-2.2%, beef extract 0.8%-1%, sterilized at 118-122°C for about 30 minutes;

[0029] Secondary seed medium: corn steep liquor 5%-7%, defoamer 0.01%, ammonium sulfate 0.3%-0.4%, dextrin 2.0%-2.2%, glucose 0.6%-0.8%, s...

Embodiment 2

[0043] The preparation of bacillus licheniformis producing keratinase comprises the following steps:

[0044] (1) Strain and culture medium preparation

[0045] Strains: keratinase strain Bacillus licheniformis, purchased from China Guangdong Microbial Culture Collection Center, the strain number is GIM1.11;

[0046] Media include slant broth media, expansion media, seed media and fermentation media;

[0047]Slope culture: broth medium composition: 0.8-1.2% beef extract, 0.8-1.2% peptone, 0.5% sodium chloride, 1.8-2.2% agar, pH7.2-7.5, sterilized at 118-122°C for about 30-40min ;

[0048] Expansion medium: 0.5% sodium chloride, 0.4%-0.6% yeast extract, 0.8%-1.2% beef extract, sterilized at 118-122°C for about 30-40min;

[0049] Primary seed medium: peptone 1.8%-2.2%, beef extract 0.8%-1%, sterilized at 118-122°C for about 30 minutes;

[0050] Secondary seed medium: corn steep liquor 5%-7%, defoamer 0.01%, ammonium sulfate 0.3%-0.4%, dextrin 2.0%-2.2%, glucose 0.6%-0.8%, st...

Embodiment 3

[0064] The preparation of bacillus licheniformis producing keratinase comprises the following steps:

[0065] (1) Strain and culture medium preparation

[0066] Strains: keratinase strain Bacillus licheniformis, purchased from China Guangdong Microbial Culture Collection Center, the strain number is GIM1.11;

[0067] Media include slant broth media, expansion media, seed media and fermentation media;

[0068] Slope culture: broth medium composition: 0.8-1.2% beef extract, 0.8-1.2% peptone, 0.5% sodium chloride, 1.8-2.2% agar, pH7.2-7.5, sterilized at 118-122°C for about 30-40min ;

[0069] Expansion medium: 0.5% sodium chloride, 0.4%-0.6% yeast extract, 0.8%-1.2% beef extract, sterilized at 118-122°C for about 30-40min;

[0070] Primary seed medium: peptone 1.8%-2.2%, beef extract 0.8%-1%, sterilized at 118-122°C for about 30 minutes;

[0071] Secondary seed medium: corn steep liquor 5%-7%, defoamer 0.01%, ammonium sulfate 0.3%-0.4%, dextrin 2.0%-2.2%, glucose 0.6%-0.8%, s...

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Abstract

The invention relates to the technical field of enzyme application, and in particular to a method for degrading alcohol soluble protein. The method comprises the step of degrading alcohol soluble protein by using keratinase. The method to prepare the low-alcohol soluble protein feed has excellent effect, can well degrade the alcohol soluble protein, and reaches protein digestibility of 90% or more, and alcohol soluble protein digestibility of 80% or more. The method fully utilizes indigestible alcohol soluble protein in the alcohol soluble protein feed, improves the feed protein digestibility,increases economic benefits of aquaculture, reduces the nitrogen content of livestock and poultry excrement, and avoids environmental nitrogen pollution caused by emission.

Description

technical field [0001] The invention relates to the technical field of enzyme application, in particular to a method for degrading gliadin. Background technique [0002] Glamin is a kind of protein in cereal protein powder that does not have enzyme function and can be dissolved in alcohol solution. It has some special physical and chemical properties so that it cannot be digested by animal endogenous enzymes. Prolamin is a protein with relatively high content in cereal protein powder. For example, cereal grain metabolic protein accounts for about 15% of the total grain protein, and gliadin and glutenin account for about 85% of the total grain protein. The protein content in corn gluten powder is as high as 40%-70%, of which zein accounts for about 68%, corn glutenin accounts for about 28%, and contains a small amount of globulin and albumin. [0003] Corn, wheat and other high prolamin raw materials are widely used in the feed industry. If the indigestible high prolamin com...

Claims

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Application Information

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IPC IPC(8): A23J3/34A23K20/147
CPCA23J3/346A23K20/147
Inventor 赵素珍刘延杰陈新胜
Owner JINANBESTZYME BIO ENG CO LTD
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