44 results about "Prolamin" patented technology

The application belongs to the field of SOD preparation, and discloses a preparation method and device of SOD resistant to pepsin degradation, which comprises the following steps: S1 powder preparation: dissolving SOD fermentation pure liquid in a buffer solution with pH of 5.2-7.8, adding protective agent and tea yellow in sequence, stirring, filtering, and placing in a freeze dryer to prepare freeze-dried powder; S2 drying; S3 tabletting; S4, coating liquid preparation: dissolving wheat gliadin and soybean protein in a mixed solution prepared from water and ethanol with a volume ratio of 1:1, wherein the mass ratio of the wheat gliadin and the soybean protein is 0.5-1:1, adding glycerol, stirring for 20 min by using a magnetic stirrer, and ultrasonicating for 40 s; S5, coating: placing the tablets in a coating machine, setting air inlet volume, air inlet temperature, pan rotating speed, atomization rate, fan face pressure, and mortar flow, until the coating weight increases to 3%-10% in mass fraction and the coating thickness is 20-50 μm, and then drying and cooling. The prepared SOD is not easy to be photolyzed and can resist gastric acid degradation to ensure the complete activity of the SOD.

The invention provides a wheat gliadin detection method, and belongs to the technical field of biology. The detection method comprises the following steps: mixing a sample to be detected with an ethanol solution to obtain a mixture; performing water bath on the mixture to obtain a mixed extract; centrifuging the mixed extract to obtain a supernatant; adding a reductive alkylation reagent into the supernate to obtain a reaction product; adding alkaline trypsin into the reaction product to carry out enzymolysis reaction to obtain an enzymolysis product; a first characteristic peptide fragment and a second characteristic peptide fragment as shown in SEQ ID NO: 1 in the sequence table are respectively used as markers, and the wheat gliadin is measured by the enzymolysis product through ultra-high performance liquid chromatography-tandem mass spectrometry by utilizing the markers. The method is simple in process and capable of efficiently detecting the wheat gliadin.

The invention belongs to the technical field of nano packaging, and relates to embedding of protein nanoparticles on vitamins, in particular to a method for embedding vitamin acetate with prolamin. Dissolving prolamin and vitamin acetate in glacial acetic acid to obtain a glacial acetic acid phase; dissolving a stabilizer in water to prepare a stabilizer phase; adding the stabilizer phase into the glacial acetic acid phase, and uniformly mixing to obtain a mixed solution; the volume of the stabilizer phase is lower than that of the glacial acetic acid phase; adding the mixed solution into water, and mixing to form the vitamin acetate-loaded prolamin particles wrapped by the stabilizer; the volume of the mixed liquid is lower than that of the water. According to the method provided by the invention, the embedding rate of the prolamin on the vitamin acetate can be improved, the release rate is slowed down, and the stability of the storage process is enhanced.

The invention discloses application of a ZmEREB93 gene knockout biological material in cultivation of high-yield and high-quality corn, and relates to the technical field of biology. The ZmEREB93 gene knockout strain is constructed, homozygous seeds of the ZmEREB93 gene knockout strain are subjected to seed test analysis, and it is found that corn kernels of the knockout strain are remarkably superior to a wild type in the aspects of the grain length, the grain width, the hundred-grain weight and the single plant yield. In the early, middle and late periods of grain filling, the corn grains of the knockout strain are obviously superior to a wild type control in shape and endosperm structure. Further determination of starch content and protein content shows that the starch content of seeds of the knockout strain is significantly higher than that of a wild type control group, the alcohol-soluble protein content is also significantly increased, and the total protein content is significantly higher than that of a control group. Therefore, the ZmEREB93 gene has an important application value in cultivation of a high-yield and high-quality new corn variety.

The invention discloses a method for preparing hollow nanoparticles from soybean protein isolate-wheat gliadin compound protein and delivering naringenin, and belongs to the technical field of food application. The method comprises the following steps: extracting soybean protein isolate, preparing a soybean protein isolate ethanol solution, preparing a wheat gliadin ethanol solution, carrying out alkaline heating, preparing hollow nanoparticles by taking sodium carbonate as a hard template, preparing a naringenin ethanol stock solution, and preparing the naringenin-loaded hollow nanoparticles. The functional characteristics of the soybean protein isolate and the wheat gliadin are improved, the requirements of the green food industry for environment-friendly and natural auxiliary materials are closely combined, a new path is provided for widening the application of plant protein in the field of green functional ingredients, and the method is simple to operate, low in cost and suitable for industrial production. And the food industry can be promoted to be transformed to a more nutritional, safer and more sustainable direction. The method for preparing the hollow nano-particle loaded naringenin by combining alkali heat treatment with an anti-solvent precipitation method lays a foundation for application of the hollow nano-particles in the food field and research in biological medicine.

A method of producing an aqueous solution of zein suitable for forming edible coatings includes combining zein with an alcohol-free, non-corrosive amphipathic carrier solvent (e.g., [1,3]-propanediol), and adding a surfactant (e.g., surfactin) thereto, forming a Phase I solution. The surfactant may be non-ionic or anionic as determined by the type of zein being used. The Phase I solution is combined with a Phase II aqueous solution including primarily water without alcohol to provide an aqueous coating solution of zein. A plasticizer (e.g., polyethylene glycol) or fatty acid (e.g., lauric acid) may be added to the Phase II solution to enhance the zein coating. The resulting coating solution may then be applied on food, and dried, to form an edible coating.

The invention discloses a preparation method of specific yolk immunoglobulin (IgY) for resisting gliadin. The preparation method comprises the following steps: immunizing and inoculating experimental laying hens, monitoring health behaviors, collecting eggs, and separating, extracting and purifying the IgY. The IgY prepared on the basis of the method can serve as a food base material to be added into low-gluten food (such as whole wheat bread and noodles), and the allergy risk caused by intake of a small amount of gluten is reduced through specific binding of the IgY and gliadin; patients are allowed to properly ingest gluten-containing food, diet diversity is improved, nutrient imbalance is avoided, and the life quality of the patients is improved. As the IgY has good thermal stability, the IgY purified by the method can still tolerate mild heating (such as baking and cooking) in food processing, and can be widely applied to various forms of products such as baked food, meal replacement powder, liquid beverage and the like; meanwhile, the acid-base stability of the antibody can ensure that the antibody keeps activity in a stomach environment after oral administration and finally reaches the intestinal tract to play a role, and the limitation that the conventional antibody is easy to digest and degrade after oral administration is broken through.

The invention belongs to the technical field of immunotherapy of allergic diseases, and provides a method for identifying dominant B cell epitopes and T cell epitopes in wheat omega-5 prolamin. According to the identification method provided by the invention, an immunoinformatics tool, a proliferation test, a degranulation test and the like are comprehensively applied, and the cell proliferation capacity, cell factor release, IgG / IgE binding capacity and degranulation capacity of T / B cell epitopes are evaluated. Results show that the T cell epitopes T1 and T2 sharing the same sequence are relatively strong in multiplication capacity, can up-regulate Th2 related cell factors, but do not have degranulation activity. Three B cell peptide fragments B3, B4 and B9 which share a common motif region QQXPQQQ (X = F, L) can promote IgG / IgE binding and degranulation capabilities. These findings would contribute to the development of epitope-based immunotherapies against wheat allergy individuals.

The invention belongs to the technical field of rice variety breeding, and particularly relates to a breeding method of a special rice variety for patients with kidney diseases and diabetes mellitus, which comprises the following specific steps: selecting a parent material: taking a low-water-soluble protein mutant variety LGC-1 introduced from a radiation breeding field of the Japanese biological resource research institute as a female parent; the Qingyou 308 rice variety bred by Institute of Rice Institute of Academy of Agricultural Sciences in Fujian Province is used as a male parent for hybridization; hybrid progenies are obtained through hybridization, selfing is carried out, progeny seeds are analyzed from F1, and seeds with low glutelin characters and high alcohol-soluble protein characters are screened out; selecting seeds with low glycemic index from the seeds with low glutelin character and high prolamin character; seeds with high amylose content are selected from the seeds with the low glycemic index. The breeding method has the advantages that the bred seeds can be suitable for kidney patients and also suitable for diabetics.

The invention relates to the technical field of food production, in particular to an automatic wheat gluten production system and a production method thereof.The production method comprises the following steps that wheat flour and water are put into a box; the main shaft rotates to drive the spiral blade to rotate, wheat flour is stirred into dough, and the dough gathers into a working cavity in the box body; acquiring the working state of the part, close to the working cavity, of the spiral blade; judging whether the current working state of the helical blade needs to adjust the volume of the working cavity and the spindle speed; when the volume of the working cavity and the rotating speed of the spindle need to be adjusted, the volume of the working cavity in the box and the rotating speed of the spindle are adjusted based on parts, close to the working cavity, of the spiral blades. According to the application, the volume of the area where the dough is located and the output power of the motor are adjusted in real time by implementing a mode of obtaining the working state of the spiral blade, and the stirring intensity is controlled based on the state of the dough, so that the denaturation probability of glutenin and gliadin during production is effectively reduced, and the product quality is improved.

Provided are a preparation method for an antibacterial gel film based on fish scale gelatin compounded with probiotics, and the use thereof. Fish scale gelatin is compounded with probiotics to prepare a gel film with excellent properties. Meanwhile, prolamin, trehalose and an amino acid are supplemented to facilitate the formation of a unique spatial structure in the fish scale gelatin, with uniform pores and a compact structure, thereby further enhancing the physicochemical properties thereof. The prepared gel film exhibits a good antibacterial effect and can be used for the storage and preservation of perishable foods. Therefore, the present invention not only achieves the utilization of fish scale waste, but also solves the problems of spoilage and deterioration caused by the microbial contamination of the prepared product, thereby achieving a significant technical effect.

The invention discloses an application of a rice phosphorus transporter gene and an encoding protein thereof in improving nutrient utilization efficiency and rice quality. According to the invention, OsPHT1 is mutated or inhibited; 1 gene expression can significantly improve nutrient utilization efficiency and rice taste quality and nutritional quality. The method is mainly embodied in that accumulation of nitrogen and phosphorus nutrients of rice is remarkably reduced, the total protein content, glutelin content, prolamin content, globulin content, albumin content and phytic acid content of rice grains are remarkably reduced, the gel consistency of the rice is increased, the reduction value is reduced, and the eating quality and the nutritional quality of the rice are further improved.

A water-soluble prolamin composition, such as a zein composition, and methods for producing the same. A method for tagging items comprising applying a plurality of non-coding DNA tags in a prolamin composition, such as a zein composition, wherein the selection of the particular taggants corresponds with a binary or nonbinary code sequence containing information about the tagged items.

The application discloses a preparation method of a nitrite emulsion inhibitor and application thereof, and belongs to the technical field of food processing. The application prepares a double-protein particle solution by mixing wheat prolamin and sodium caseinate, then adds the double-protein particle solution into a mixed oil phase containing Chinese litse oil, and obtains a Pickering emulsion containing Chinese litse oil through a homogenization reaction. In addition, the prepared emulsion is added into meat paste, the lipid oxidation and spoilage of the meat paste are improved, the accumulation of nitrite in the meat paste is obviously inhibited during cold storage, the purposes of inhibiting bacteria and inhibiting the residue of nitrite in the meat paste during storage and improving the quality of the meat paste are realized, and finally high-quality meat paste with rich nutritional components and low nitrite residue is obtained. Moreover, the process provided by the application is simple, energy-saving and environment-friendly, and the large-scale production process is easy to implement, so the application can be widely promoted and has wide application prospects.

The invention discloses a primer probe combination for detecting wheat and peanut allergens based on a dual Proofman-LMTIA technology and application of the primer probe combination, and belongs to the technical field of molecular detection. The primer probe combination comprises an XM-F1, an XM-B1, an XM-LB1, an HS-F2, an HS-B2, an HS-LB2, an XM-Probe1 and an HS-Probe2 which are as shown in SEQ ID NO. 1 to 8. The invention further discloses a kit for detecting the content of the XM-B1 in the kit. On the basis of a Proofman-LMTIA technology, specific primers and probes for wheat and peanut allergens are designed, a rapid detection method for wheat allergen gamma-gliadin and peanut allergen Arah 2.01 protein is established, and the method is used for detecting whether the wheat and peanut allergens are mixed in the links of production, processing and circulation of processed food. And technical support is provided for improving effective supervision of food supervision departments on allergen-containing products.

The present disclosure provides systems and methods for the recovery of protein species from wet mill grain process streams. Systems and methods of the present disclosure may be integrated with a wet mill grain process to separate out protein species that may limit efficiency of the grain process and produce one or more product streams comprising these separated protein species. A feed stream may be fractionated by at least two membranes into retentate and permeate streams. Removing larger proteins through the membrane fractionation may allow previously soluble prolamin products in the permeate stream(s) to precipitate. The recovered protein species may include prolamin, such as zein from a corn grain feed.

The application belongs to the technical field of immunochromatographic detection, and particularly relates to a detection method of residual wheat-specific protein in rice products. The detection method comprises the following steps: step S1: preparing an antibody; step S2: preparing an immunochromatographic test paper; step S3: preparing a developing solution; and step S4: wheat-specific protein detection. The application significantly improves the detection sensitivity and specificity of wheat prolamin by optimizing the formula of the developing solution to screen inhibitory proteins, synergizing with surfactants, and combining with the preparation process of multiple batches of antibodies.

The present invention relates to a transgenic wheat event, which we have designated T258, that leads to reduced gliadin content, as well as cells, seeds and plants that comprise the event. The invention also relates to methods for detecting the presence of the T258 event, probes and primers for use in the method and methods of breeding with T258 to produce wheat with reduced gliadin content.

The application belongs to the technical field of plant genetic engineering, and particularly relates to a millet gene Seita.8G189900 and Seita.8G190200 application in controlling prolamine quality. The nucleotide sequence of the millet gene Seita.8G189900 is shown as SEQ ID NO. 1, and the nucleotide sequence of the millet gene Seita.8G190200 is shown as SEQ ID NO. 2. The application proves two genes that can down-regulate the content of prolamine in millet, and the prolamine content of millet can be reduced by reducing the expression level of the genes. Knocking out the two genes can not only down-regulate the content of prolamine, but also up-regulate the content of amino acid, increase the nutritional value of millet, and be more beneficial to gastrointestinal absorption and digestion, thereby providing a new gene resource for protein quality improvement.

This invention discloses a gene that regulates the content of gliadin in rice grains. qPro7 This invention relates to the fields of genetic engineering and plant molecular breeding, and its applications. The amino acid sequence of the gene is shown in SEQ ID NO.1. Through genome-wide association analysis, this invention identified and cloned the gene on rice chromosome 7. qPro7 By constructing genes qPro7 Knockout vectors and self-promoted overexpression vectors were used to transform rice, and the results showed that knockout vectors... qPro7 The gene can significantly reduce the content of prolactin in rice grains, while overexpression qPro7 The gene significantly increases the content of prolamins in rice grains; this invention provides new genetic resources for the genetic improvement of rice quality, especially the regulation of protein content, and has important application value.

The utility model relates to an automatic device for extracting alcohol soluble protein from distillers' grains. The automatic device aims at efficiently extracting the alcohol soluble protein from the distillers' grains. The process comprises the following steps: receiving bran-containing vinasse from a vinasse pool, heating the vinasse into dry vinasse through a combustion pool, and conveying the dry vinasse to a screening device through a first conveyor belt; the screening device separates bran hulls from vinasse, the bran hulls are conveyed to the combustion pool through the second conveying belt, and the vinasse is stored in the storage bin. The vinasse in the storage bin is pulverized into powder by the pulverizing machine, the powder is conveyed to the stirring device and stirred in the alcohol solution, so that the alcohol-soluble protein is dissolved into the solution, and insoluble waste is also conveyed to the combustion pool through the second conveying belt. The alcohol solution containing the alcohol-soluble protein is stored in the pre-storage tank and then enters the drying machine, alcohol steam and the alcohol-soluble protein are separated out, and the alcohol-soluble protein is stored in the finished product storage tank. Alcohol steam is converted into alcohol liquid through the condensing device, the alcohol liquid is stored in the alcohol reflux tank, and the alcohol liquid flows back to the stirring device to be recycled after the concentration and the volume of the alcohol solution are adjusted through the storage tank. According to the device, all links are tightly matched, the full-automatic process from vinasse receiving, treatment and alcohol-soluble protein extraction to alcohol recycling is achieved, the alcohol-soluble protein extraction efficiency is improved, waste and alcohol are reasonably recycled, the cost is reduced, meanwhile, environmental pollution is reduced, and remarkable economic and environment-friendly benefits are achieved.

The invention belongs to the technical field of colloid and interface chemistry, and discloses a Pickering double emulsion and a preparation method thereof.The preparation method comprises the steps that enzymatic oxidation lignin is prepared on the basis of ball-milling activated lignin, alcohol-soluble protein-enzymatic oxidation lignin turbid liquid is prepared on the basis of alcohol-soluble protein ethanol water and the enzymatic oxidation lignin, and the Pickering double emulsion is obtained. A ternary composite nano dispersion liquid is prepared based on a carboxymethylated beta-glucan aqueous solution and a prolamin-enzymatic oxidation lignin suspension, an oil cake precipitate is obtained based on a curable oil phase and the ternary composite nano dispersion liquid, and a Janus particle dispersion liquid is prepared based on the oil cake precipitate and 2-morpholinoethanesulfonic acid. Obtaining an inner water phase / oil phase / outer water phase type Pickering double emulsion based on the Janus particle dispersion liquid, the inner water phase, the oil phase and the outer water phase; the method disclosed by the invention can show excellent stability and interface regulation and control capability.

The application provides a modification method of millet prolamin, uniformly mixing the millet prolamin with a solvent to obtain a millet prolamin solution; uniformly mixing a cationic surfactant with the millet prolamin solution to obtain a spinning solution; and electrospinning the spinning solution to obtain a millet prolamin nanofiber product. The cationic surfactant is added in the millet prolamin solution for modification, and the millet prolamin nanofiber product is successfully prepared, the mechanical strength and water stability of the nanofiber product are greatly improved, and the application of the millet prolamin is widened.

The invention relates to a natural component-based hydrophobic finishing agent for goose down and a preparation method thereof, and the hydrophobic finishing agent for goose down is prepared from the following raw materials in parts by weight: 35-45 parts of ethyl cellulose, 12-18 parts of plukenetia volubilis extract, 15-20 parts of highland barley alcohol-soluble protein, 10-15 parts of sorghum alcohol-soluble protein, 5-7 parts of tung oil, 2-5 parts of citric acid and 3-8 parts of castor oil. The plukenetia volubilis extract is obtained by taking stems and leaves of plukenetia volubilis as raw materials and extracting with an organic solvent. The plukenetia volubilis extract, the highland barley alcohol-soluble protein and the sorghum alcohol-soluble protein are added into a hydrophobic finishing agent system to be compounded for use, so that the antibacterial property and the hydrophobic property of the goose down can be remarkably improved, and the antibacterial property and the hydrophobic property can still be kept at a high level after being washed for multiple times; therefore, the three components have a certain synergistic effect.

The application discloses a starch-based nano intelligent label, a preparation method and application thereof in visual monitoring of meat freshness. The method uses debranched starch, prolamin and anthocyanin as raw materials, fully stirs and mixes, and then is ultrasonically homogenized. After centrifugation, the composite coagulation layer is taken as a spinning solution, and then the starch-based nano intelligent label is prepared through electrospinning. The average fiber diameter of the starch-based nano intelligent label is 450-600 nm, and the water contact angle is 100-120 DEG. After the starch-based nano intelligent label is applied to meat product freshness monitoring after volatile acid fumigation, the color of the starch-based nano intelligent label presents color changes of red, pink and purple corresponding to the aggravation of the meat product corruption degree, the ΔE value is greater than 5, and the visual monitoring of the meat freshness is realized in a food-grade safe and reliable manner.

The kit (100) comprises a preparation solution (17) configured to prepare a compound to be analyzed, a sensor (1) for determining the gluten contamination in the compound and a processing unit connected thereto. The sensor comprises a base layer (2) having a pair of electrodes (2a); an upper layer (4) having a membrane (4a) permeable to at least one gas releasable from the compound according to the concentration of at least one prolamin present; and a resistive layer (3) interposed between the base layer (2) and the upper layer (4) and configured to detect the gas released by the compound and permeating through the membrane (4a) and to change its resistivity according to the gas detected. The pair of electrodes (2a) is configured to detect the change in resistivity in the resistive layer (3) and to generate a signal according to the change in resistivity detected.

The invention discloses a method for detecting gluten allergen alcohol-soluble protein based on multiple molecularly imprinted polymer enrichment. The method comprises the following steps: sample enzymolysis, preparation of multiple molecularly imprinted polymers, and enrichment and purification of multiple target molecules. Performing LC-MS / MS (liquid chromatography-mass spectrometry / mass spectrometry) analysis, quantification and confirmation: respectively quantifying the three peptide fragments by using an external standard method until the concentration of any peptide fragment is gt; if the concentration is 1.0 mu g / kg, determining that the sample is gluten positive. According to the prepared multi-molecularly imprinted polymer, three peptide fragments serve as common templates, the multi-molecularly imprinted polymer capable of recognizing three structures at the same time is synthesized at a time, one-time synchronous enrichment of main gluten sensitizers is achieved, enzymolysis and multi-MISPE enrichment are converted into LC-MS / MS multi-target analysis, and the multi-molecular imprinted polymer has the advantages of being simple in preparation method, low in cost and good in application prospect. The excellent selective enrichment capacity of the multi-molecularly imprinted polymer is combined with the high specificity and high-sensitivity quantitative capacity of LC-MS / MS, and the problems of insufficient sensitivity and matrix interference of trace gluten protein detection in a complex matrix are solved.

The present disclosure belongs to the technical field of functional ingredient extraction, and provides preparation and application of a distiller's grains arabinoxylan-sorghum prolamin complex. The preparation method comprises the following steps: drying and crushing distiller's grains, removing oil and starch, obtaining a suspension in an alkaline environment through infrared irradiation, stirring, shearing and centrifuging the suspension to obtain a supernatant, adjusting the supernatant to be acidic, centrifuging the supernatant again, alcohol precipitation, drying to obtain an extract, and coupling reaction at a proper temperature and humidity. The present disclosure also discloses the complex prepared by the above method, an emulsifier comprising the complex, and application of the emulsifier in the fields of food and cosmetics. The present disclosure adds infrared-assisted extraction, enhances dissolution and extraction of polysaccharides and sorghum prolamin, shortens extraction time and improves extraction rate; and further enhances emulsification through a dry heating method. The prepared complex has good water solubility, fast dissolution speed and strong emulsifying capacity, and can be used as a natural emulsifier in various scenarios.

The invention discloses an application of a Zm00001d029512 gene and a protein coded by the Zm00001d029512 gene in regulation and control of corn protein quality, and belongs to the technical field of plant genetic engineering. The CDS (Coding Sequence) of the Zm00001d029512 gene is as shown in SEQ ID NO. 1 (Sequence Identifier Number 1). According to the invention, the Zm00001d029512 gene is knocked out from corn, so that the accumulation proportion of prolamin in corn kernel endosperm can be effectively reduced, and meanwhile, the content of essential amino acids including lysine is remarkably increased. Experimental data prove that after the function of the gene is lost, the transcriptional level of various alcohol-soluble protein components is remarkably reduced, and the proportion of non-alcohol-soluble protein in grains is correspondingly increased, so that the composition of stored protein is fundamentally optimized, and the core nutritional defect of unbalanced amino acid composition in corn protein is directly solved. The invention provides a direct target for cultivating a new high-quality corn variety, and has important breeding application value.

The invention belongs to the technical field of functional component extraction, and provides preparation and application of a vinasse araboxylan-sorghum prolamin compound. The preparation method comprises the following steps: drying and crushing the vinasse, and then carrying out degreasing and starch removal treatment; performing infrared irradiation in an alkaline environment to obtain turbid liquid; stirring, shearing and centrifuging the turbid liquid to obtain supernate, adjusting the supernate to be acidic, centrifuging to obtain supernate, performing alcohol precipitation, and drying to obtain an extract; and carrying out coupling reaction on the extract at proper temperature and humidity. The invention also discloses the compound prepared by the method, an emulsifier comprising the compound, and application of the emulsifier in the fields of food and cosmetics. Infrared-assisted extraction is added, so that dissolution and extraction of polysaccharide and sorghum prolamin are enhanced, the extraction time is shortened, and the extraction rate is increased; and the emulsifying property is further enhanced through a dry heating method. The prepared compound is good in water solubility, high in dissolving speed and high in emulsifying capacity, and can be used as a natural emulsifier in various scenes.