67 results about "Prolamin" patented technology

The invention belongs to the technical field of cereal protein processing, and particularly relates to wheat gliadin with good color and luster and glutenin with high solubility as well as a preparation method and application of the wheat gliadin and the glutenin. According to the method, a cold water solubility control coupling one-step freezing purification technology is broken through, the concentration of ethanol and the temperature of the solution are controlled through cold water, the dissolving state and aggregation behavior of wheat gliadin are synchronously regulated and controlled, then freezing treatment and a one-step method are coupled to achieve controllable protein aggregation, precipitation and freezing, and the purposes of separation and purification are achieved. The technology is green, efficient, simple, convenient and energy-saving, the obtained alcohol-soluble protein is good in color and luster, and industrial production is easy to realize. Furthermore, a membrane separation coupling high-pressure microjet technology is broken through, and on the basis of a technology for obtaining a glutenin concentrated solution through membrane separation, the protein concentrated solution is directly treated by adopting an alkaline condition synchronous high-pressure microjet circulating modification method, so that the structural characteristics such as the scale and the surface hydrophobicity of the target protein and the aggregation behavior are further regulated and controlled; the glutenin is endowed with better solubility.

The invention relates to the technical field of plant genetic engineering, and particularly provides a rice endosperm tissue specific promoter pPROLM26 and application thereof. According to the invention, a tissue-specific promoter with a nucleotide sequence as shown in SEQ ID NO.1 is separated from a rice alcohol-soluble protein gene, the tissue-specific promoter is cut off for four times, and then the cut-off promoter fragment with an endosperm-specific promoter function as shown in SEQ ID NO.2-5 is obtained after molecular identification is carried out on the cut-off promoter fragment and an RUBY visual marker; the betaine content of the expression product can reach 2.5% of the dry weight of the seeds through determination, and the method has very high application value in the field of plant synthetic biology. The invention further discloses application of the promoter or the truncated promoter fragment in the aspects of improving crop seed quality, improving crop traits, cultivating new varieties of transgenic plants by using key elements of the promoter or expressing high value-added proteins by using a seed bioreactor and the like.

A method for modifying a gliadin and an application thereof are provided. The method includes the following steps: dissolving the gliadin in a solvent to obtain gliadin solution; and adding a proline endoprotease to the gliadin solution for a reaction, and adjusting the pH value of solution to neutral according to a gradient, to obtain a modified gliadin. The modified gliadin obtained by the present invention may be uniformly dispersed in water, and may still maintain a uniform dispersion state in the water after high-temperature thermal sterilization and long-term storage, or even in the presence of sodium chloride, it has good thermal stability, salt resistance, and storage stability.

The invention belongs to the field of food engineering and the field of biological macromolecular materials, and particularly relates to a preparation method and application of oleogel based on protein fibers. The oleogel is prepared from the prolamin from barley and different protein fiber and xanthan gum compounds, the obtained oleogel has high oil holding capacity, high storage modulus, better thixotropic resilience and good temperature and thermodynamic stability, and different types of oleogels can be suitable for different processing scenes, so that the oleogel can be applied to different processing fields. The method can be widely applied to the fields of foods, medicines and cosmetics.

The invention provides a construction method and an application method of a wheat yeast two-hybrid cDNA library, and belongs to the technical field of biology. The invention provides a construction method of a yeast two-hybrid cDNA library, which comprises the following steps: extracting total RNA, separating mRNA, converting into cDNA, transferring into escherichia coli competent cells through a series of reactions to obtain a cDNA primary library bacterial liquid, separating primary library plasmids, and obtaining a yeast two-hybrid cDNA library bacterial liquid through an LR recombination reaction and conversion of the escherichia coli competent cells. A high-quality wheat variety Zheng wheat 158 is taken as a research material, wheat gliadin genes Tagli-gamma-1 and Tagli-gamma-11 are cloned, a corresponding bait vector is constructed, the interaction protein is screened from a cDNA library of the Zheng wheat 158 by utilizing a yeast two-hybrid technology, and a foundation is laid for carrying out interaction protein screening of genes related to wheat quality and analyzing a regulation mechanism of the interaction protein in a later period.

The application belongs to the field of SOD preparation, and discloses a preparation method and device of SOD resistant to pepsin degradation, which comprises the following steps: S1 powder preparation: dissolving SOD fermentation pure liquid in a buffer solution with pH of 5.2-7.8, adding protective agent and tea yellow in sequence, stirring, filtering, and placing in a freeze dryer to prepare freeze-dried powder; S2 drying; S3 tabletting; S4, coating liquid preparation: dissolving wheat gliadin and soybean protein in a mixed solution prepared from water and ethanol with a volume ratio of 1:1, wherein the mass ratio of the wheat gliadin and the soybean protein is 0.5-1:1, adding glycerol, stirring for 20 min by using a magnetic stirrer, and ultrasonicating for 40 s; S5, coating: placing the tablets in a coating machine, setting air inlet volume, air inlet temperature, pan rotating speed, atomization rate, fan face pressure, and mortar flow, until the coating weight increases to 3%-10% in mass fraction and the coating thickness is 20-50 μm, and then drying and cooling. The prepared SOD is not easy to be photolyzed and can resist gastric acid degradation to ensure the complete activity of the SOD.

The invention provides a wheat gliadin detection method, and belongs to the technical field of biology. The detection method comprises the following steps: mixing a sample to be detected with an ethanol solution to obtain a mixture; performing water bath on the mixture to obtain a mixed extract; centrifuging the mixed extract to obtain a supernatant; adding a reductive alkylation reagent into the supernate to obtain a reaction product; adding alkaline trypsin into the reaction product to carry out enzymolysis reaction to obtain an enzymolysis product; a first characteristic peptide fragment and a second characteristic peptide fragment as shown in SEQ ID NO: 1 in the sequence table are respectively used as markers, and the wheat gliadin is measured by the enzymolysis product through ultra-high performance liquid chromatography-tandem mass spectrometry by utilizing the markers. The method is simple in process and capable of efficiently detecting the wheat gliadin.

A method for modifying a gliadin and an application thereof are provided. The method includes the following steps: dissolving the gliadin in a solvent to obtain gliadin solution; and adding a proline endoprotease to the gliadin solution for a reaction, and adjusting the pH value of solution to neutral according to a gradient, to obtain a modified gliadin. The modified gliadin obtained by the present invention may be uniformly dispersed in water, and may still maintain a uniform dispersion state in the water after high-temperature thermal sterilization and long-term storage, or even in the presence of sodium chloride, it has good thermal stability, salt resistance, and storage stability.

The invention discloses a preparation method and application of grape cluster-like prolamin / cellulose nano-composite particles. The preparation method comprises the following steps: preparing a nano-cellulose aqueous dispersion; preparing an alcohol-soluble protein alcohol-water solution; the preparation method comprises the following steps: preparing grape-cluster-shaped nano composite particles: mixing a nano cellulose aqueous dispersion with an alcohol-soluble protein alcohol-water solution, adding water into the obtained mixture to separate out and deposit alcohol-soluble protein on the surface of nano cellulose, and heating and evaporating to remove alcohol to obtain a grape-cluster-shaped alcohol-soluble protein / cellulose nano composite particle aqueous dispersion. The method is simple, the cost is low, the sustainable development requirement is met, the size and the number of the prolamin nanoparticles on the surface of the nanocellulose have certain controllability, meanwhile, the obtained nano composite particles have good emulsifying capacity, stable Pickering emulsion can be prepared, and the method is suitable for the fields of cosmetics, food, medicine and the like.

The invention discloses a preparation method of wheat gliadin / soybean protein isolate nanoparticle embedded astaxanthin, and belongs to the field of functional food. The preparation process comprises the following steps: (1) weighing wheat gliadin and soybean protein isolate according to different proportions; (2) dissolving the samples in different proportions in distilled water; (3) stirring the mixed liquid for 1 hour under the condition of 600 r / min; (4) carrying out ultrasonic dispersion treatment on the protein solution to promote the expansion of the protein structure; (5) adding the astaxanthin ethanol solution into the protein solution, and stirring for 1 hour under the condition of 600 r / min; (6) slowly adjusting the pH value of the mixed system to 7.0 by using a 1M citric acid solution; and (7) carrying out centrifugal treatment on the mixed system, and taking supernate to obtain the G / SPI nano-particles and the G / SPI / ASTA nano-particles. According to the preparation method, the double-protein nano-particles prepared by adopting a PH driving method have good stability, the embedding rate of astaxanthin is greatly improved, internal astaxanthin can be effectively protected from being damaged by the external environment, and it is indicated that the double-protein nano-particles have potential application prospects in carrying of bioactive substances.

The invention belongs to the technical field of nano packaging, and relates to embedding of protein nanoparticles on vitamins, in particular to a method for embedding vitamin acetate with prolamin. Dissolving prolamin and vitamin acetate in glacial acetic acid to obtain a glacial acetic acid phase; dissolving a stabilizer in water to prepare a stabilizer phase; adding the stabilizer phase into the glacial acetic acid phase, and uniformly mixing to obtain a mixed solution; the volume of the stabilizer phase is lower than that of the glacial acetic acid phase; adding the mixed solution into water, and mixing to form the vitamin acetate-loaded prolamin particles wrapped by the stabilizer; the volume of the mixed liquid is lower than that of the water. According to the method provided by the invention, the embedding rate of the prolamin on the vitamin acetate can be improved, the release rate is slowed down, and the stability of the storage process is enhanced.

The invention discloses application of a ZmEREB93 gene knockout biological material in cultivation of high-yield and high-quality corn, and relates to the technical field of biology. The ZmEREB93 gene knockout strain is constructed, homozygous seeds of the ZmEREB93 gene knockout strain are subjected to seed test analysis, and it is found that corn kernels of the knockout strain are remarkably superior to a wild type in the aspects of the grain length, the grain width, the hundred-grain weight and the single plant yield. In the early, middle and late periods of grain filling, the corn grains of the knockout strain are obviously superior to a wild type control in shape and endosperm structure. Further determination of starch content and protein content shows that the starch content of seeds of the knockout strain is significantly higher than that of a wild type control group, the alcohol-soluble protein content is also significantly increased, and the total protein content is significantly higher than that of a control group. Therefore, the ZmEREB93 gene has an important application value in cultivation of a high-yield and high-quality new corn variety.

The invention discloses a method for separating a hordein B-component by utilizing iota-carrageenan. The method comprises the following steps: step 1, extracting hordein; step 2, pretreating protein and polysaccharide; 3, preparing a protein / polysaccharide complex coacervation precipitate based on electrostatic interaction; step 4, removing iota-carrageenan, removing iota-carrageenan precipitate at the bottom layer, and collecting supernate, so as to obtain a B-hordein solution; and step five, freeze-drying B-hordein: collecting a B-hordein solution, removing ethanol by using a rotary evaporator, freezing a sample, and freeze-drying to obtain B-hordein protein powder. According to the method, the B-hordein can be separated in a low-loss and high-selectivity manner, the operation process is simple, the equipment requirement is low, large-scale production is easy, the dosage of iota-carrageenan is small, the iota-carrageenan can be recycled through membrane separation, and the C-hordein in the hordein can be recycled at the same time.

The invention discloses a method for preparing hollow nanoparticles from soybean protein isolate-wheat gliadin compound protein and delivering naringenin, and belongs to the technical field of food application. The method comprises the following steps: extracting soybean protein isolate, preparing a soybean protein isolate ethanol solution, preparing a wheat gliadin ethanol solution, carrying out alkaline heating, preparing hollow nanoparticles by taking sodium carbonate as a hard template, preparing a naringenin ethanol stock solution, and preparing the naringenin-loaded hollow nanoparticles. The functional characteristics of the soybean protein isolate and the wheat gliadin are improved, the requirements of the green food industry for environment-friendly and natural auxiliary materials are closely combined, a new path is provided for widening the application of plant protein in the field of green functional ingredients, and the method is simple to operate, low in cost and suitable for industrial production. And the food industry can be promoted to be transformed to a more nutritional, safer and more sustainable direction. The method for preparing the hollow nano-particle loaded naringenin by combining alkali heat treatment with an anti-solvent precipitation method lays a foundation for application of the hollow nano-particles in the food field and research in biological medicine.

The invention provides an application of a TaMYB46L-5A protein or a gene for coding the TaMYB46L-5A protein in regulating and controlling the protein content of plant seeds, and belongs to the technical field of molecular biology. The invention provides an application of a TaMYB46L-5A protein or a gene for coding the TaMYB46L-5A protein in at least one of regulation and control of protein content of plant seeds, creation of transgenic plants with dominant protein content and breeding of plant varieties with dominant protein content. The result of the embodiment of the invention shows that the seed protein content of a TaMYB46L-5A overexpression plant is obviously reduced, the seed protein content of a TaMYB46L-5A mutant plant is increased, and the contents of prolamin and glutenin are obviously increased. Therefore, the TaMYB46L-5A can negatively regulate and control the protein content of the plant seeds, and plays an important role in plant breeding.

A method of producing an aqueous solution of zein suitable for forming edible coatings includes combining zein with an alcohol-free, non-corrosive amphipathic carrier solvent (e.g., [1,3]-propanediol), and adding a surfactant (e.g., surfactin) thereto, forming a Phase I solution. The surfactant may be non-ionic or anionic as determined by the type of zein being used. The Phase I solution is combined with a Phase II aqueous solution including primarily water without alcohol to provide an aqueous coating solution of zein. A plasticizer (e.g., polyethylene glycol) or fatty acid (e.g., lauric acid) may be added to the Phase II solution to enhance the zein coating. The resulting coating solution may then be applied on food, and dried, to form an edible coating.

The invention discloses a preparation method of specific yolk immunoglobulin (IgY) for resisting gliadin. The preparation method comprises the following steps: immunizing and inoculating experimental laying hens, monitoring health behaviors, collecting eggs, and separating, extracting and purifying the IgY. The IgY prepared on the basis of the method can serve as a food base material to be added into low-gluten food (such as whole wheat bread and noodles), and the allergy risk caused by intake of a small amount of gluten is reduced through specific binding of the IgY and gliadin; patients are allowed to properly ingest gluten-containing food, diet diversity is improved, nutrient imbalance is avoided, and the life quality of the patients is improved. As the IgY has good thermal stability, the IgY purified by the method can still tolerate mild heating (such as baking and cooking) in food processing, and can be widely applied to various forms of products such as baked food, meal replacement powder, liquid beverage and the like; meanwhile, the acid-base stability of the antibody can ensure that the antibody keeps activity in a stomach environment after oral administration and finally reaches the intestinal tract to play a role, and the limitation that the conventional antibody is easy to digest and degrade after oral administration is broken through.

The invention belongs to the technical field of immunotherapy of allergic diseases, and provides a method for identifying dominant B cell epitopes and T cell epitopes in wheat omega-5 prolamin. According to the identification method provided by the invention, an immunoinformatics tool, a proliferation test, a degranulation test and the like are comprehensively applied, and the cell proliferation capacity, cell factor release, IgG / IgE binding capacity and degranulation capacity of T / B cell epitopes are evaluated. Results show that the T cell epitopes T1 and T2 sharing the same sequence are relatively strong in multiplication capacity, can up-regulate Th2 related cell factors, but do not have degranulation activity. Three B cell peptide fragments B3, B4 and B9 which share a common motif region QQXPQQQ (X = F, L) can promote IgG / IgE binding and degranulation capabilities. These findings would contribute to the development of epitope-based immunotherapies against wheat allergy individuals.

The invention belongs to the technical field of rice variety breeding, and particularly relates to a breeding method of a special rice variety for patients with kidney diseases and diabetes mellitus, which comprises the following specific steps: selecting a parent material: taking a low-water-soluble protein mutant variety LGC-1 introduced from a radiation breeding field of the Japanese biological resource research institute as a female parent; the Qingyou 308 rice variety bred by Institute of Rice Institute of Academy of Agricultural Sciences in Fujian Province is used as a male parent for hybridization; hybrid progenies are obtained through hybridization, selfing is carried out, progeny seeds are analyzed from F1, and seeds with low glutelin characters and high alcohol-soluble protein characters are screened out; selecting seeds with low glycemic index from the seeds with low glutelin character and high prolamin character; seeds with high amylose content are selected from the seeds with the low glycemic index. The breeding method has the advantages that the bred seeds can be suitable for kidney patients and also suitable for diabetics.

The invention relates to the technical field of food production, in particular to an automatic wheat gluten production system and a production method thereof.The production method comprises the following steps that wheat flour and water are put into a box; the main shaft rotates to drive the spiral blade to rotate, wheat flour is stirred into dough, and the dough gathers into a working cavity in the box body; acquiring the working state of the part, close to the working cavity, of the spiral blade; judging whether the current working state of the helical blade needs to adjust the volume of the working cavity and the spindle speed; when the volume of the working cavity and the rotating speed of the spindle need to be adjusted, the volume of the working cavity in the box and the rotating speed of the spindle are adjusted based on parts, close to the working cavity, of the spiral blades. According to the application, the volume of the area where the dough is located and the output power of the motor are adjusted in real time by implementing a mode of obtaining the working state of the spiral blade, and the stirring intensity is controlled based on the state of the dough, so that the denaturation probability of glutenin and gliadin during production is effectively reduced, and the product quality is improved.

The present invention belongs to the technical field of microbial engineering, and particularly relates to a Bacillus subtilis for enhancing the available protein in alcoholic corn distillers grains and the preparation and application of its composite bacterium agent. The present invention provides a Bacillus subtilis with a preservation number of CGMCC No. 32291. The Bacillus subtilis of the present invention can secrete protease and cellulase, degrade macromolecular proteins in alcoholic corn distillers grains into small molecular weight proteins during fermentation, and reduce the content of prolamin. The present invention also provides a composite bacterium agent, which includes Lactobacillus plantarum and the Bacillus subtilis described in the above technical solution. The composite bacterium agent of the present invention can increase the viable bacteria count and the content of corresponding beneficial metabolites in fermented alcoholic corn distillers grains, enrich the nutrition of alcoholic corn distillers grains, and improve the utilization rate of alcoholic corn distillers grains.

Provided are a preparation method for an antibacterial gel film based on fish scale gelatin compounded with probiotics, and the use thereof. Fish scale gelatin is compounded with probiotics to prepare a gel film with excellent properties. Meanwhile, prolamin, trehalose and an amino acid are supplemented to facilitate the formation of a unique spatial structure in the fish scale gelatin, with uniform pores and a compact structure, thereby further enhancing the physicochemical properties thereof. The prepared gel film exhibits a good antibacterial effect and can be used for the storage and preservation of perishable foods. Therefore, the present invention not only achieves the utilization of fish scale waste, but also solves the problems of spoilage and deterioration caused by the microbial contamination of the prepared product, thereby achieving a significant technical effect.

This specification relates to an active substance carrier comprising a core-shell network structure formed by using a biopolymer. In one aspect of this specification, the core-shell network structure comprises core-shell particles composed of a core containing prolamin; and a shell containing pullulan and pectin. The pullulan contained in the shell surrounds the core, and the pectin is located on the outermost layer of the shell and forms an interconnected network between the core-shell particles, thereby effectively promoting the transdermal absorption of the active substance.

The invention discloses an application of a rice phosphorus transporter gene and an encoding protein thereof in improving nutrient utilization efficiency and rice quality. According to the invention, OsPHT1 is mutated or inhibited; 1 gene expression can significantly improve nutrient utilization efficiency and rice taste quality and nutritional quality. The method is mainly embodied in that accumulation of nitrogen and phosphorus nutrients of rice is remarkably reduced, the total protein content, glutelin content, prolamin content, globulin content, albumin content and phytic acid content of rice grains are remarkably reduced, the gel consistency of the rice is increased, the reduction value is reduced, and the eating quality and the nutritional quality of the rice are further improved.

A water-soluble prolamin composition, such as a zein composition, and methods for producing the same. A method for tagging items comprising applying a plurality of non-coding DNA tags in a prolamin composition, such as a zein composition, wherein the selection of the particular taggants corresponds with a binary or nonbinary code sequence containing information about the tagged items.

The application discloses a preparation method of a nitrite emulsion inhibitor and application thereof, and belongs to the technical field of food processing. The application prepares a double-protein particle solution by mixing wheat prolamin and sodium caseinate, then adds the double-protein particle solution into a mixed oil phase containing Chinese litse oil, and obtains a Pickering emulsion containing Chinese litse oil through a homogenization reaction. In addition, the prepared emulsion is added into meat paste, the lipid oxidation and spoilage of the meat paste are improved, the accumulation of nitrite in the meat paste is obviously inhibited during cold storage, the purposes of inhibiting bacteria and inhibiting the residue of nitrite in the meat paste during storage and improving the quality of the meat paste are realized, and finally high-quality meat paste with rich nutritional components and low nitrite residue is obtained. Moreover, the process provided by the application is simple, energy-saving and environment-friendly, and the large-scale production process is easy to implement, so the application can be widely promoted and has wide application prospects.

The invention discloses a primer probe combination for detecting wheat and peanut allergens based on a dual Proofman-LMTIA technology and application of the primer probe combination, and belongs to the technical field of molecular detection. The primer probe combination comprises an XM-F1, an XM-B1, an XM-LB1, an HS-F2, an HS-B2, an HS-LB2, an XM-Probe1 and an HS-Probe2 which are as shown in SEQ ID NO. 1 to 8. The invention further discloses a kit for detecting the content of the XM-B1 in the kit. On the basis of a Proofman-LMTIA technology, specific primers and probes for wheat and peanut allergens are designed, a rapid detection method for wheat allergen gamma-gliadin and peanut allergen Arah 2.01 protein is established, and the method is used for detecting whether the wheat and peanut allergens are mixed in the links of production, processing and circulation of processed food. And technical support is provided for improving effective supervision of food supervision departments on allergen-containing products.

The present disclosure provides systems and methods for the recovery of protein species from wet mill grain process streams. Systems and methods of the present disclosure may be integrated with a wet mill grain process to separate out protein species that may limit efficiency of the grain process and produce one or more product streams comprising these separated protein species. A feed stream may be fractionated by at least two membranes into retentate and permeate streams. Removing larger proteins through the membrane fractionation may allow previously soluble prolamin products in the permeate stream(s) to precipitate. The recovered protein species may include prolamin, such as zein from a corn grain feed.

The invention relates to the field of food preservation, and particularly discloses a highland barley vinasse prolamin-based preservation coating liquid and a preparation method, and every 100 mL of the preservation coating liquid comprises 1800-2200 mg of highland barley vinasse prolamin, 1.3-1.6 mL of a humectant, 2.2-2.7 g of vitamin C and 75-85 mL of an alcoholic solution. The prepared film coating liquid can obviously reduce the weight loss rate of the fruits in the storage period and effectively reduce the water loss; the decay rate is obviously inhibited, and an experimental group does not have a decay phenomenon within a 28-day storage period; the fruit hardness is effectively maintained, and the hardness reduction speed is slowed down; change of titratable acid content is controlled, organic acid reduction is delayed, and fruit flavor is reserved; the activity of polyphenol oxidase is obviously inhibited, the enzymatic browning reaction is retarded, and the color and quality of fruits are maintained; the total number of bacterial colonies is greatly reduced, and growth and reproduction of microorganisms are inhibited, so that the shelf life of fruits is effectively prolonged.

The application belongs to the technical field of immunochromatographic detection, and particularly relates to a detection method of residual wheat-specific protein in rice products. The detection method comprises the following steps: step S1: preparing an antibody; step S2: preparing an immunochromatographic test paper; step S3: preparing a developing solution; and step S4: wheat-specific protein detection. The application significantly improves the detection sensitivity and specificity of wheat prolamin by optimizing the formula of the developing solution to screen inhibitory proteins, synergizing with surfactants, and combining with the preparation process of multiple batches of antibodies.