The invention discloses a preparation method of succinic-anhydride-modified corn prolamin drug-loading particles. The method comprises the following steps of: dissolving corn prolamin in dimethyl sulfoxide or ethanol aqueous solution, regulating pH of the solution to 10-11, then mixing with succinic anhydride, reacting for 1-2 hours at a temperature of 40-45 DEG C, keeping the pH of the solution being alkaline in the reaction process, obtaining succinylated corn prolamin liquid, placing the liquid in a dialysis bag for dialysis after reaction is ended, freezing and drying into powdery solid, and dissolving hydrophobic drugs and freeze-dried succinylated corn prolamin in the ethanol aqueous solution; filling hydrochloric-acid aqueous solution under stirring, and after stabilizing, centrifuging to remove supernatant, thus obtaining the drug-loading particles. The preparation method disclosed by the invention has the beneficial effects that the prepared drug-loading particles are high insphericity degree, good in dispersity, narrow in particle-size distribution and uniform in size, the stability is obviously improved compared with unmodified corn prolamin particles under the physiological pH environment, the slow release effect is good, and the prepared drug-loading particles can be used for oral or sucking type administration or be prepared into suspensions for injection use.