Preparation method of proteins separated from a corn germ
A corn germ and protein separation technology, applied in the field of food processing, can solve problems such as rare reports, and achieve the effects of high extraction rate and simple preparation method.
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[0011] The method utilizes water, 5% sodium sulfite solution, 70% ethanol solution and 0.4% sodium hydroxide to step-by-step extract albumin, globulin, alcohol-soluble protein and alkali-soluble protein in corn germ, specifically comprising the following steps:
[0012] (1) Weigh 5g of defatted corn germ, add water to dissolve it, preheat it in a water bath, and put it in a constant temperature magnetic stirrer at 40°C to shake the protein to dissolve the protein in the liquid, centrifuge at 4000r / min for 20min-30min, and collect The supernatant is albumin;
[0013] (2) Take the residue extracted by water in step (1), add 5% sodium sulfite solution, and after fully dissolving, preheat in a water bath at a temperature of 40°C and place it in a constant temperature magnetic stirrer for shaking to dissolve the protein in In the liquid, centrifuge at 4000r / min for 30min, collect the supernatant, which is the globulin;
[0014] (3) Add 70% ethanol solution to the residue of step (...
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