118 results about "Genetically modified maize" patented technology

The invention belongs to the technical field of genetic engineering, and particularly relates to a method for creating a corn dwarfing material and application of the corn dwarfing material. The invention discloses a method for creating a corn dwarfing material, which specifically comprises the following steps: carrying out specific key site editing on a target gene ZmGA20ox3 and / or ZmGA20ox5 in corn by using a genetic engineering means, and carrying out specific amino acid site mutation on a protein coded by the target gene ZmGA20ox3 and / or ZmGA20ox5 subjected to specific key site editing, and further cultivating and screening to obtain the corn dwarfing material. The method provided by the invention can be used for cultivating transgenic corn plants with different plant heights and other agronomic traits, and meanwhile, a new breeding technical scheme is provided for creating dwarf corn, reducing the height of a corn ear, reducing the included angle of corn plant leaves and increasing the corn yield; crops can be more densely planted in agriculture, so that the yield of the crops in unit area is improved, and the method has important breeding significance.

The invention discloses an effect protein E56, a coding gene and application of the effect protein E56 to improvement of southern rust resistance of corn, and belongs to the technical field of agricultural biology. The amino acid sequence of the effector protein E56 is as shown in SEQ ID NO. 1. A reverse genetics method is utilized to analyze the Puccinia polystachys effect protein E56 gene, and the E56 gene is up-regulated in expression in the Puccinia polystachys infection process. The E56 gene is silenced by adopting a host-mediated gene silencing technology, and the toxic function of the E56 gene in the infection process of the southern rust disease is determined. A specific fragment of the E56 gene is cloned to an RNAi interference vector, a corn immature embryo is transformed by using an agrobacterium tumefaciens-mediated transgenic technology, and an obtained transgenic corn plant shows resistance to the corn southern rust disease. The E56-RNAi transgenic plant shows resistance to puccinia polypoda, so that the effect protein E56 can be used for creating a new strain for resisting the southern rust disease.

The invention provides a primer pair, a kit, a PCR (Polymerase Chain Reaction) detection method and application of the PCR detection method in detection of transgenic maize LD05.

The invention provides an application of a corn disease-resistant gene ZmCRK10 and a disease-resistant molecular marker thereof in improvement of corn stem rot resistance, and belongs to the technical field of plant genetic engineering. The invention provides application of a disease-resistant gene ZmCRK10 or a disease-resistant protein ZmCRK10-T03 in improvement of corn stem rot resistance. The disease-resistant gene ZmCRK10 or the disease-resistant protein ZmCRK10-T03 can be used for constructing a transgenic corn variety resistant to corn stem rot. The invention further provides application of the disease-resistant molecular marker ZmCRK10TypeB of the corn disease-resistant gene ZmCRK10 in improving the corn stem rot resistance, and effective auxiliary molecular marker selection can be provided for corn stem rot resistance breeding.

The invention relates to a nucleic acid molecule for detecting a transgenic maize event DBN9641 containing a universal hybrid seed production (UHT) and a detection method of the nucleic acid molecule, and the nucleic acid molecule comprises SEQ ID NO: 1 or a complementary sequence of the SEQ ID NO: 1, and SEQ ID NO: 2 or a complementary sequence of the SEQ ID NO: 2. The corn plant DBN9641 is endowed with a function of keeping sterility of a sterile line in a hybrid seed production technology, so that a castration step in a hybrid seed production process is omitted, manpower and material resources are saved, the operation is simple, and the detection method can accurately and quickly identify whether a biological sample contains DNA molecules of the transgenic corn event DBN9641 or not.

The invention relates to a corn transformation event MN85zN-Ex nucleic acid molecule and a detection method. The corn transformation event MN85zN-Ex nucleic acid molecule comprises a sequence shown in SEQ ID NO: 1 or a reverse complementary sequence thereof, or a sequence shown in SEQ ID NO: 2 or a reverse complementary sequence thereof. The corn transformation event MN85zN-Ex has the characteristics of insect resistance and glufosinate-ammonium herbicide resistance, and the detection method can accurately and quickly identify whether a biological sample contains the DNA molecule of the transgenic corn event MN85zN-Ex or not.

The invention discloses a primer group for homozygosity and heterozygosity identification of transgenic maize CC-2 and a detection method. The invention provides a primer pair group. The primer pair group consists of a primer pair 1 (SEQ ID No.1 and SEQ ID No.2) and a primer pair 2 (SEQ ID No.3 and SEQ ID No.2), the primer pair 1 is used for specifically detecting whether to-be-detected corn is transgenic corn CC-2 or not; the primer pair 2 is used for detecting whether the insertion of an exogenous target gene maroACC in the transgenic maize CC-2 is heterozygous or homozygous. Two pairs of primers are designed for the transgenic maize CC-2, a set of PCR detection method for homozygosis and heterozygosis of the transgenic maize CC-2 is established, and the PCR detection method is of great significance to screening of homozygous transformants and improvement of seed purity and quality in the breeding process.

The invention discloses a transgenic corn insect-resistant effect evaluation method, which comprises the following steps: constructing a standardized planting system, adopting a special powder-flocculent feed to realize unified inoculation of multiple insects, standardizing survey indexes and calculation logic, and finishing data processing and insect-resistant grade judgment in combination with a Python automatic analysis system. According to the method, a complete evaluation system of standardized planting, unified insect inoculation, unified investigation and automatic analysis is formed, the insect inoculation efficiency of the method is improved by 17 times compared with that of a traditional method, the data processing efficiency of the method is improved by 1620 times compared with that of an Excel + SPSS combination, standardized technical support is provided for safety evaluation, variety certification and industrial popularization of transgenic maize, and the method has important practical application value.

The invention belongs to the technical field of herbicides, and particularly relates to a herbicide for preventing and removing weeds in transgenic corn and soybean fields. A herbicide for preventing and removing weeds in transgenic corn and soybean fields comprises an active component and an auxiliary component, and the active component is formed by compounding glyphosate or glufosinate-ammonium and Epyrifenacil according to the mass ratio of (1-80): (80-1). After glyphosate or glufosinate-ammonium is compounded with Epyrifenacil, the control effect on eleusine indica, abutilon fiber and the like can be improved, and the herbicide controlling spectrum is expanded. Besides, the herbicide active component is formed by compounding two weeding active components according to a certain proportion, compared with a single-action-mechanism herbicide, the herbicide resistance evolution of weeds can be effectively controlled, the service life of the herbicide can be prolonged, and then important support is provided for scientific and effective prevention and control of the weeds.

The invention discloses a primer probe combination and a kit for on-site detection of a transgenic maize transformant, and relates to the technical field of transgenic detection. The invention provides a primer probe combination for on-site detection of a transgenic maize transformant. The primer probe combination comprises a primer pair and a probe for detecting a transgenic maize strain MON810, the primer pair and the probe are used for detecting the transgenic maize strain TC1507; the primer pair and the probe are used for detecting the transgenic maize strain Bt11; the invention discloses a primer pair and a probe for detecting corn internal reference zSSIIb. The method for detecting the transgenic maize by adopting the primer probe combination and the kit disclosed by the invention integrates sample pretreatment, nucleic acid extraction, purification, amplification and detection, has better sensitivity and accuracy, is simple, convenient, rapid and time-saving to operate, and plays an important role in on-site rapid detection of transgenic maize research and safety management.

The present invention discloses a method for increasing the yield of maize. The method is to overexpress an auxin influx carrier protein in maize, screen for transformed plants with significantly increased ear row number, grain weight per ear, or test weight, and increase the yield of maize. Overexpression of the auxin transport protein in the present invention can significantly increase the test weight and ear row number of maize kernels in inbred lines and hybrids, thereby significantly increasing the yield. Under the same genetic background, both the test weight and ear row number of kernels are positively correlated with the yield of maize. Selecting transgenic maize into which the auxin transport protein gene has been introduced using the test weight and ear row number can effectively obtain high-yield transgenic maize.

The invention discloses a primer group for homozygosity and heterozygosity identification of transgenic maize ND207 and a detection method. The invention provides a primer pair group. The primer pair group consists of a primer pair 1 (SEQ ID No.1 and SEQ ID No.2) and a primer pair 2 (SEQ ID No.3 and SEQ ID No.4); the primer pair 1 is used for specifically detecting whether to-be-detected corn is transgenic corn ND207 or not; the primer pair 2 is used for detecting whether the insertion of an exogenous target gene in the transgenic maize ND207 is heterozygous or homozygous. Two pairs of primers are designed for the transgenic maize ND207, a set of PCR detection method for homozygosis and heterozygosis of the transgenic maize ND207 is established, and the PCR detection method is of great significance to screening of homozygous transformants and improvement of seed purity and quality in the breeding process.

The invention relates to the field of transgenic plants, in particular to a nucleic acid molecule, a primer group and application thereof. The present invention provides a nucleic acid molecule having a nucleotide sequence as any of SEQ ID NO: 1 to SEQ ID NO: 7. The invention provides a left flanking sequence and a right flanking sequence of an exogenous insertion vector of an insect-resistant and herbicide-resistant corn WYN8158 transformation event and application of the left flanking sequence and the right flanking sequence in detection of transgenic corn, and can be used for rapidly, efficiently and specifically detecting whether a sample to be detected is derived from a transgenic component or not. Meanwhile, technical support is provided for market identification and identification after commercialization and industrialization of the product, and meanwhile the method has important significance and wide prospects for promoting corn germplasm resource identification in China.

The invention discloses an insect-resistant and herbicide-resistant transgenic maize event JZL22k-1 and a detection method of the insect-resistant and herbicide-resistant transgenic maize event JZL22k-1. The transgenic maize event JZL22k-1 nucleic acid sequence comprises SEQ ID NO: 1 or a complementary sequence thereof, and / or SEQ ID NO: 2 or a complementary sequence thereof; the transgenic maize plant JZL22k-1 has good resistance to lepidoptera insects and good tolerance to glyphosate herbicides and has no influence on the yield, and the detection method can accurately and stably identify the existence of a JZL22k-1 transgenic event by applying a nucleic acid amplification method. The method is of great significance in implementation of research of JZL22k-1, traceability of production processing and application and whole-process supervision.

This invention discloses the application of LNA1 protein and related biological materials in regulating the number of leaves on the ear and yield of maize. The invention first involves overexpression of LNA1 protein in wild-type maize. lna1 , received lna1 Gene-modified maize and statistical analysis of its leaf number revealed that overexpression... lna1 It can increase the number of leaves on the corn ear and the total number of leaves. To further verify the function of LNA1, this invention knocked out LNA1 in wild-type corn. lna1, get lna1 Knockout corn and statistical analysis of its leaf number and plant height revealed that: lna1 It can reduce the number of leaves on the corn ear and the plant height. Furthermore, this invention has demonstrated improved efficiency by constructing NIL-Popcorn and NIL-MS71. lna1 Gene expression levels can increase maize yield. These results indicate that the LNA1 protein and related biological materials can regulate leaf number, yield, and plant height, and will play an important role in breeding high-yielding maize varieties.

The present invention belongs to the field of molecular biology technology, and specifically relates to a transgenic corn event LP1141-1 and a detection method thereof. The present invention provides a nucleic acid sequence for detecting transgenic corn event LP1141-1, wherein the nucleic acid sequence is selected from one or more of the sequences SEQ ID NO: 1-7 or their complementary sequences, and the nucleic acid sequence is derived from transgenic corn event LP1141-1. Representative samples of seeds of the transgenic corn event LP1141-2 have been deposited in the China Center for Type Culture Collection on April 13, 2025, with a deposit number of CCTCC NO: P202511. The transgenic corn event LP1141-1 of the present invention has the triple traits of obtaining red maintainer seeds, breeding sterile materials, and glufosinate-ammonium herbicide resistance. The event simplifies the screening method and saves labor costs; does not affect corn yield; and can track transgenic inserts in the breeding population and its progeny using molecular markers. The detection method provided by the present invention can quickly, accurately, and stably identify the presence of plant material derived from the transgenic corn event LP1141-1.

The invention relates to the field of transgenic plants, in particular to a nucleic acid molecule, a primer group and application thereof. The present invention provides a nucleic acid molecule having a nucleotide sequence as any of SEQ ID NO: 1 to SEQ ID NO: 7. The invention provides a left flanking sequence and a right flanking sequence of an exogenous insertion vector of an insect-resistant and herbicide-resistant corn WYN8158 transformation event and application of the left flanking sequence and the right flanking sequence in detection of transgenic corn, and can be used for rapidly, efficiently and specifically detecting whether a sample to be detected is derived from a transgenic component or not. Meanwhile, technical support is provided for market identification and identification after commercialization and industrialization of the product, and meanwhile the method has important significance and wide prospects for promoting corn germplasm resource identification in China.

The invention belongs to the technical field of molecular biological detection, and particularly relates to a primer group and a method for detecting homozygote and heterozygote of transgenic maize LP026-2. The homozygote and heterozygote of the transgenic maize LP026-2 are distinguished by adopting a KASP (Competitive Allele Polymerase Chain Reaction) technology. According to the reaction, two possible genotypes of a detection sample in each reaction hole are subjected to two-color fluorescence reaction to reflect whether the genotype of the transgenic maize LP026-2 is a wild type, a homozygote or a heterozygote. The method has the advantages that high-throughput and high-sensitivity genotyping is realized, the method is suitable for LP026-2 derived varieties with different genetic backgrounds, the foreground selection requirements of large-scale samples in the backcross transformation process are met, and a technical template is provided for detection of other transgenic crops.

The invention belongs to the technical field of plant breeding, and relates to a transgenic maize event LP111-2 and a detection method thereof. The invention provides a nucleic acid molecule for detecting a transgenic maize event LP111-2, the sequence of the nucleic acid molecule is as shown in SEQ ID NO: 1-2, SEQ ID NO: 3-4, SEQ ID NO: 5 or one or more groups of complementary sequences thereof, the nucleic acid molecule is derived from the transgenic maize event LP111-2, and the sequence of the nucleic acid molecule is as shown in SEQ ID NO: 1-2, SEQ ID NO: 3-4, SEQ ID NO: 5 or one or more groups of complementary sequences thereof. Maize seeds comprising the transgenic maize event LP111-2 have been preserved in the China Center for Type Culture Collection (CCTCC) with a preservation number of CCTCC NO: P202430. Maize plants comprising the transgenic maize event LP111-2 of the present invention have the following advantages: free of economic losses due to lepidoptera pests; an agricultural herbicide containing glyphosate can be applied to corn crops; the corn yield is not reduced; and the breeding efficiency is enhanced, and the molecular marker can be used for tracking transgenic insertion fragments in a breeding population and a progeny thereof.

The application discloses a kind of evaluation Spodoptera frugiperda to corn ear damage method, belong to harmful insect ecological control technical field.The application first proposes a kind of new method by measuring corn ear bending angle to judge the damage degree of Spodoptera frugiperda to corn ear, specifically: corn milk ripening period, select the corn damaged by Spodoptera frugiperda, and measure corn ear bending angle, according to corn ear bending angle, the damaged condition of corn yield is evaluated.The application can not only efficiently, quickly and objectively judge the damage degree of corn ear, but also solve the problems of high difficulty in area measurement and large error caused by irregular damaged parts of corn ear in the existing evaluation method, provide a scientific basis for transgenic corn insect resistance evaluation, and have important significance for improving Spodoptera frugiperda control technology system.

The invention relates to an exogenous insertion sequence of a glyphosate and glufosinate-ammonium resistant corn transformation event LE4, a detection method and application. A nucleic acid molecule sequence of the corn LE4 comprises SEQ ID NO.1 or SEQ ID NO.2 or SEQ ID NO.13 or a reverse complementary sequence of the SEQ ID NO.1 or SEQ ID NO.2 or SEQ ID NO.13. The invention further relates to a method for detecting the glyphosate and glufosinate-ammonium resistant corn transformation event LE4. The corn transformation event LE4 has glyphosate and glufosinate herbicide resistance and excellent agronomic traits, and the detection method can accurately and quickly identify whether a biological sample contains DNA molecules of the transgenic corn event LE4 or not.

Modified corn event MON95275 and plants, plant cells, seeds, plant parts, progeny plants, commodity products comprising detectable amounts of a modified corn event MON95275 DNA are provided. Polynucleotides and sequences specific for a modified corn event MON95275, methods for making a modified corn event MON95275, methods for making and using plants, plant cells, seeds, plant parts, progeny plants, and commodity products comprising a modified corn event MON95275, and methods for detecting a modified corn event MON95275, and polynucleotides or DNA sequences specific for a modified corn event MON95275, in a DNA molecule or sample, are provided.

The invention discloses a method for creating a male sterile line and a maintainer line of maize based on a CRISPR-Cas12i. 3 system and application of the method. The invention belongs to the field of genetic breeding, and particularly relates to a method for creating a male sterile line and a maintainer line of maize based on a CRISPR-Cas12i. 3 system and application of the method. The method for preparing the transgenic corn comprises the following steps: mutating an Ms26 gene of a receptor corn genome to cause Ms26 protein function deletion to obtain the transgenic corn, and the transgenic corn has at least one of the following characteristics: (1) compared with the receptor corn, the tassel of the transgenic corn shows compact spikelet, tight glume protection and incapability of normal pollen scattering, and the transgenic corn has the characteristics that the Ms26 protein function deletion is caused by the mutation of the Ms26 gene of the receptor corn genome; no pollen is exposed; and (2) compared with the receptor corn, the transgenic corn is completely aborted. By establishing an efficient backcross transformation method, the genic male sterile line without transgenic ingredients is rapidly obtained, the breeding period is greatly shortened, and the application cost is reduced.

The present application belongs to the technical field of agricultural environmental science, and particularly relates to a transgenic corn gene drift prediction method based on wind speed dominance, which collects environmental parameters of wind speed, wind direction, humidity, atmospheric stability, positioning, range and altitude, corn variety specificity parameters and historical gene drift observation data in a target area; constructs a Gaussian plume diffusion model based on the preprocessed data to determine a gene drift frequency atlas; under a Bayesian neural field framework, the gene drift frequency atlas is taken as input, the actually measured gene drift data is taken as model observation values, and the preprocessed environmental parameters and corn variety specificity parameters are taken as covariates to optimize the model, so as to output a predicted gene drift flow threshold distance and give a risk prevention and control report. The present application combines the advantages of physical models and empirical models, overcomes the defects that the prior art does not fully consider the differences in varieties and environmental factors, and realizes accurate prediction of gene drift.

The invention discloses a chitin synthase 1 gene and application thereof in prevention and treatment of diplodia juncoides, and belongs to the technical field of genetic engineering and biological prevention and treatment. The nucleotide sequence of the chitin synthetase 1 gene is as shown in SEQ ID NO. 1. The invention also provides dsRNA (double-stranded ribonucleic acid) for preventing and treating diplodia diplosa, and the dsRNA is formed by transcription of the nucleotide sequence as shown in SEQ ID NO.3. The invention discloses the chitin synthetase 1 gene of the diplodia juncoides for the first time, and designs dsRNA capable of preventing and treating the diplodia juncoides and transgenic corn for expressing the dsRNA of the diplodia juncoides based on the gene. By feeding the transgenic corn leaves, the death rate of the leucotina diplosa can be remarkably increased, and the leaf damage area and degree can be reduced. The invention provides a new gene resource and an efficient and environment-friendly prevention and control method for prevention and control of the diplodia juncoides, provides a new choice for prevention and control of the diplodia juncoides, and has a wide application prospect.

The invention provides a transgenic corn event MON95275, plants, plant cells, seeds, plant parts (including pollen, seed, and cells, and tissues corresponding to tassel, root, stalk, stem, leaf, cobb, and the like), progeny plants, commodity products comprising detectable amounts of corn event MON95275 DNA. The invention also provides polynucleotides specific for corn event MON95275 and methods for using and detecting corn event MON95275 DNA as well as plants, plant cells, seeds, plant parts, progeny plants, and commodity products comprising corn event MON95275. The invention also provides methods related to making and using corn event MON95275.

A novel transgenic corn elite event designated MZIR260 is disclosed. The invention relates to DNA sequences of the recombinant constructs inserted into the corn genome and of genomic sequences flanking the insertion site that resulted in elite event MZIR260. The invention further relates to assays for detecting the presence of the DNA sequences of corn elite event MZIR260, to corn plants and corn seeds comprising the genotype thereof, and to methods for producing a corn plant by crossing a corn plant comprising the elite event MZIR260 genotype with itself or another corn variety.

Aspergillus flavus is an opportunistic, saprophytic fungus that infects maize and other fatty acid-rich food and feed crops and produces toxic and carcinogenic secondary metabolites known as aflatoxins. In vitro studies showed a five-fold increase in antifungal activity of AGM182 (vs. tachyplesin1) against A. flavus. Transgenic maize plants expressing AGM182 under maize Ubiquitin-1 promoter were produced through Agrobacterium-mediated transformation. PCR products confirmed integration of the AGM182 gene, while RT-PCR of maize RNA confirmed the presence of AGM182 transcripts. Maize kernel screening assay using a highly aflatoxigenic A. flavus strain (AF70) showed up to 72% reduction in fungal growth in the transgenic AGM182 seeds compared to isogenic negative control seeds.

The invention relates to a composition for detecting corn nucleic acid and application of the composition, belongs to the field of biological detection, and particularly relates to a real-time fluorescent quantitative detection system and a lateral flow chromatography sensor constructed by recombinase polymerase nucleic acid amplification and CRISPR / Cas12b through a simple and easy-to-operate process of single temperature change. The two-step reaction of RPA and CRISPR / Cas12b is sequentially carried out in the same reaction tube, so that the mutual interference of RPA and CRISPR / Cas12b is solved. SgRNA is designed according to an RPA product of a specific sequence of a transgenic corn DBN9936 transformant, and whether a to-be-detected sample contains a transgenic corn component or not can be rapidly detected according to an end point fluorescence value of a real-time fluorescence PCR instrument or a detection result of reading a lateral flow chromatography test strip by utilizing trans-cleavage activity of a reaction between a base complementation principle and CRISPR / Cas12b (Clustered Regularly Interspaced Short Palindromic Repeats / CRISPR / Cas12b).