50 results about "Genetically modified maize" patented technology

The invention discloses a transgenic corn insect-resistant effect evaluation method, which comprises the following steps: constructing a standardized planting system, adopting a special powder-flocculent feed to realize unified inoculation of multiple insects, standardizing survey indexes and calculation logic, and finishing data processing and insect-resistant grade judgment in combination with a Python automatic analysis system. According to the method, a complete evaluation system of standardized planting, unified insect inoculation, unified investigation and automatic analysis is formed, the insect inoculation efficiency of the method is improved by 17 times compared with that of a traditional method, the data processing efficiency of the method is improved by 1620 times compared with that of an Excel + SPSS combination, standardized technical support is provided for safety evaluation, variety certification and industrial popularization of transgenic maize, and the method has important practical application value.

The invention discloses an insect-resistant and herbicide-resistant transgenic maize event JZL22k-1 and a detection method of the insect-resistant and herbicide-resistant transgenic maize event JZL22k-1. The transgenic maize event JZL22k-1 nucleic acid sequence comprises SEQ ID NO: 1 or a complementary sequence thereof, and / or SEQ ID NO: 2 or a complementary sequence thereof; the transgenic maize plant JZL22k-1 has good resistance to lepidoptera insects and good tolerance to glyphosate herbicides and has no influence on the yield, and the detection method can accurately and stably identify the existence of a JZL22k-1 transgenic event by applying a nucleic acid amplification method. The method is of great significance in implementation of research of JZL22k-1, traceability of production processing and application and whole-process supervision.

This invention discloses the application of LNA1 protein and related biological materials in regulating the number of leaves on the ear and yield of maize. The invention first involves overexpression of LNA1 protein in wild-type maize. lna1 , received lna1 Gene-modified maize and statistical analysis of its leaf number revealed that overexpression... lna1 It can increase the number of leaves on the corn ear and the total number of leaves. To further verify the function of LNA1, this invention knocked out LNA1 in wild-type corn. lna1, get lna1 Knockout corn and statistical analysis of its leaf number and plant height revealed that: lna1 It can reduce the number of leaves on the corn ear and the plant height. Furthermore, this invention has demonstrated improved efficiency by constructing NIL-Popcorn and NIL-MS71. lna1 Gene expression levels can increase maize yield. These results indicate that the LNA1 protein and related biological materials can regulate leaf number, yield, and plant height, and will play an important role in breeding high-yielding maize varieties.

The invention relates to the field of transgenic plants, in particular to a nucleic acid molecule, a primer group and application thereof. The present invention provides a nucleic acid molecule having a nucleotide sequence as any of SEQ ID NO: 1 to SEQ ID NO: 7. The invention provides a left flanking sequence and a right flanking sequence of an exogenous insertion vector of an insect-resistant and herbicide-resistant corn WYN8158 transformation event and application of the left flanking sequence and the right flanking sequence in detection of transgenic corn, and can be used for rapidly, efficiently and specifically detecting whether a sample to be detected is derived from a transgenic component or not. Meanwhile, technical support is provided for market identification and identification after commercialization and industrialization of the product, and meanwhile the method has important significance and wide prospects for promoting corn germplasm resource identification in China.

The application discloses a kind of evaluation Spodoptera frugiperda to corn ear damage method, belong to harmful insect ecological control technical field.The application first proposes a kind of new method by measuring corn ear bending angle to judge the damage degree of Spodoptera frugiperda to corn ear, specifically: corn milk ripening period, select the corn damaged by Spodoptera frugiperda, and measure corn ear bending angle, according to corn ear bending angle, the damaged condition of corn yield is evaluated.The application can not only efficiently, quickly and objectively judge the damage degree of corn ear, but also solve the problems of high difficulty in area measurement and large error caused by irregular damaged parts of corn ear in the existing evaluation method, provide a scientific basis for transgenic corn insect resistance evaluation, and have important significance for improving Spodoptera frugiperda control technology system.

The invention discloses a method for creating a male sterile line and a maintainer line of maize based on a CRISPR-Cas12i. 3 system and application of the method. The invention belongs to the field of genetic breeding, and particularly relates to a method for creating a male sterile line and a maintainer line of maize based on a CRISPR-Cas12i. 3 system and application of the method. The method for preparing the transgenic corn comprises the following steps: mutating an Ms26 gene of a receptor corn genome to cause Ms26 protein function deletion to obtain the transgenic corn, and the transgenic corn has at least one of the following characteristics: (1) compared with the receptor corn, the tassel of the transgenic corn shows compact spikelet, tight glume protection and incapability of normal pollen scattering, and the transgenic corn has the characteristics that the Ms26 protein function deletion is caused by the mutation of the Ms26 gene of the receptor corn genome; no pollen is exposed; and (2) compared with the receptor corn, the transgenic corn is completely aborted. By establishing an efficient backcross transformation method, the genic male sterile line without transgenic ingredients is rapidly obtained, the breeding period is greatly shortened, and the application cost is reduced.

The present application belongs to the technical field of agricultural environmental science, and particularly relates to a transgenic corn gene drift prediction method based on wind speed dominance, which collects environmental parameters of wind speed, wind direction, humidity, atmospheric stability, positioning, range and altitude, corn variety specificity parameters and historical gene drift observation data in a target area; constructs a Gaussian plume diffusion model based on the preprocessed data to determine a gene drift frequency atlas; under a Bayesian neural field framework, the gene drift frequency atlas is taken as input, the actually measured gene drift data is taken as model observation values, and the preprocessed environmental parameters and corn variety specificity parameters are taken as covariates to optimize the model, so as to output a predicted gene drift flow threshold distance and give a risk prevention and control report. The present application combines the advantages of physical models and empirical models, overcomes the defects that the prior art does not fully consider the differences in varieties and environmental factors, and realizes accurate prediction of gene drift.

The invention discloses a chitin synthase 1 gene and application thereof in prevention and treatment of diplodia juncoides, and belongs to the technical field of genetic engineering and biological prevention and treatment. The nucleotide sequence of the chitin synthetase 1 gene is as shown in SEQ ID NO. 1. The invention also provides dsRNA (double-stranded ribonucleic acid) for preventing and treating diplodia diplosa, and the dsRNA is formed by transcription of the nucleotide sequence as shown in SEQ ID NO.3. The invention discloses the chitin synthetase 1 gene of the diplodia juncoides for the first time, and designs dsRNA capable of preventing and treating the diplodia juncoides and transgenic corn for expressing the dsRNA of the diplodia juncoides based on the gene. By feeding the transgenic corn leaves, the death rate of the leucotina diplosa can be remarkably increased, and the leaf damage area and degree can be reduced. The invention provides a new gene resource and an efficient and environment-friendly prevention and control method for prevention and control of the diplodia juncoides, provides a new choice for prevention and control of the diplodia juncoides, and has a wide application prospect.

The invention provides a transgenic corn event MON95275, plants, plant cells, seeds, plant parts (including pollen, seed, and cells, and tissues corresponding to tassel, root, stalk, stem, leaf, cobb, and the like), progeny plants, commodity products comprising detectable amounts of corn event MON95275 DNA. The invention also provides polynucleotides specific for corn event MON95275 and methods for using and detecting corn event MON95275 DNA as well as plants, plant cells, seeds, plant parts, progeny plants, and commodity products comprising corn event MON95275. The invention also provides methods related to making and using corn event MON95275.

The invention relates to a composition for detecting corn nucleic acid and application of the composition, belongs to the field of biological detection, and particularly relates to a real-time fluorescent quantitative detection system and a lateral flow chromatography sensor constructed by recombinase polymerase nucleic acid amplification and CRISPR / Cas12b through a simple and easy-to-operate process of single temperature change. The two-step reaction of RPA and CRISPR / Cas12b is sequentially carried out in the same reaction tube, so that the mutual interference of RPA and CRISPR / Cas12b is solved. SgRNA is designed according to an RPA product of a specific sequence of a transgenic corn DBN9936 transformant, and whether a to-be-detected sample contains a transgenic corn component or not can be rapidly detected according to an end point fluorescence value of a real-time fluorescence PCR instrument or a detection result of reading a lateral flow chromatography test strip by utilizing trans-cleavage activity of a reaction between a base complementation principle and CRISPR / Cas12b (Clustered Regularly Interspaced Short Palindromic Repeats / CRISPR / Cas12b).

The present application provides a nucleic acid sequence comprising one or more selected from the sequences SEQ ID NO: 1-7 and their complements, which is derived from a plant, seed or cell comprising corn event LP026-5, a representative sample of the seed comprising the event has been deposited under accession number CCTCC NO: P202210. The transgenic corn event LP026-5 of the present application not only has good resistance to the feeding of Lepidoptera pests, but also can tolerate agricultural herbicides containing glyphosate. The corn plants with this double trait have the advantages of being free from economic losses caused by Lepidoptera pests, being able to tolerate the commonly used commercial herbicide glyphosate in corn crops, not reducing corn yield, enhancing breeding efficiency, and being able to track the transgenic insert in the breeding population and its offspring with molecular markers. Meanwhile, the detection method provided by the present application can quickly, accurately and stably identify the presence of plant materials derived from the transgenic corn event LP026-5.

A novel transgenic corn elite event designated MZIR260 is disclosed. The invention relates to DNA sequences of the recombinant constructs inserted into the corn genome and of genomic sequences flanking the insertion site that resulted in elite event MZIR260. The invention further relates to assays for detecting the presence of the DNA sequences of corn elite event MZIR260, to corn plants and corn seeds comprising the genotype thereof, and to methods for producing a corn plant by crossing a corn plant comprising the elite event MZIR260 genotype with itself or another corn variety.

This invention discloses a chitin synthase 1 gene and its application in controlling the two-spotted leaf beetle, belonging to the fields of genetic engineering and biological control technology. The nucleotide sequence of the chitin synthase 1 gene is shown in SEQ ID NO.1. This invention also provides a dsRNA for controlling the two-spotted leaf beetle, transcribed from the nucleotide sequence shown in SEQ ID NO.3. This invention discloses for the first time the chitin synthase 1 gene of the two-spotted leaf beetle, and designs a dsRNA based on this gene capable of controlling the two-spotted leaf beetle, as well as transgenic maize expressing the two-spotted leaf beetle dsRNA. Feeding transgenic maize leaves significantly increases the mortality rate of the two-spotted leaf beetle and reduces the area and severity of leaf damage. This invention provides a new genetic resource and an efficient and environmentally friendly control method for the two-spotted leaf beetle, offering a new option for its control and possessing broad application prospects.

The present application relates to a method for improving corn yield and lignin content by overexpressing sucrose synthase gene. The method is to overexpress sucrose synthase gene ZmSUS1 in corn to obtain transgenic corn with high yield and high lignin content; the nucleotide sequence of the sucrose synthase gene ZmSUS1 is shown as SEQ ID NO. 1. The present application overexpresses sucrose synthase gene ZmSUS1 in corn, effectively provides precursor substances for the biosynthesis of starch and lignin, so that the corn overexpressing sucrose synthase gene ZmSUS1 has total starch content increased by 3.5-13.5%, yield increased by 19.0%-30.3%, and lignin content increased by 2.84-3.15% compared with normal corn plants, significantly improving the total starch content of corn, the yield of corn and the lignin content in straw.

The invention relates to the technical field of biosensors, in particular to a transgenic corn CP4-EPSPS protein aptamer molecular beacon fluorescent biosensor. According to the invention, a systematic aptamer rational cutting and conformation adaptation strategy is constructed, the strategy obtains an initial aptamer through magnetic bead-SELEX screening, and the CP4-EPSPS protein aptamer is precisely cut and optimized by adopting the steps of loop region active core positioning, stem region structure optimization and base binding domain identification, so that the binding performance and the structural stability are remarkably improved. Subsequently, on the basis of stable conformational characteristics, a complementary chain competition sensing mechanism is designed, and a transgenic corn CP4-EPSPS protein aptamer molecular beacon fluorescent biosensor is successfully constructed, so that amplification-free high-sensitivity rapid detection of CP4-EPSPS protein is realized, and a solution is provided for supervision of transgenic crops.

This invention discloses a P450 gene of crabgrass from paddy fields and its application in conferring resistance to cyhalofop-butyl in plants. The expression level of this gene in the resistant crabgrass biotype is significantly higher than that in the sensitive biotype. By constructing it into a plant expression vector and transforming it into maize, the resulting transgenic maize plants were sprayed with 11.25 g ai ha at the 3-4 leaf stage. ‑1 After treatment with cyhalofop-butyl, the maize exhibited significantly higher herbicide resistance and survival rate than wild-type maize. This invention confirms that the crabgrass P450 gene is directly related to herbicide resistance, providing a foundation for using this gene to breed transgenic crops resistant to ACCase inhibitor herbicides, and has broad application prospects in the field of agricultural biotechnology.

The invention belongs to the technical field of molecular biology, and particularly relates to a flanking sequence of an exogenous insertion element of an insertion site of herbicide-resistant transgenic maize EP81803 and application of the flanking sequence. The invention provides a flanking sequence of an exogenous insertion element of an insertion site of herbicide-resistant transgenic maize EP81803. The flanking sequence comprises a 5'end flanking sequence and / or a 3 'end flanking sequence; the 5'end flanking sequence is a sequence as shown in SEQ ID NO. 5; the 3'end flanking sequence is a sequence as shown in SEQ ID NO.6. The transgenic corn EP81803 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.30709. Specific detection and identification can be performed on the transgenic corn EP81803 and related materials based on the flanking sequence, and the transgenic corn can be better supervised and managed.

The application discloses a method for preparing maize haploid. The method comprises the following steps: (1) reducing the activity and / or expression amount of protein ZmGEX1 in diploid maize to obtain transgenic maize; the protein ZmGEX1 is a protein with an amino acid sequence as shown in SEQ ID NO: 3; (2) selfing the transgenic maize or crossing the transgenic maize with diploid maize as a female parent to obtain offspring; and separating maize haploid from the offspring. The application firstly utilizes the protein ZmGEX1 to prepare maize haploid, avoids the phenotype of weak growth and poor fertility of an induction system, and can prepare haploid by selfing, so that excellent crop traits are completely reserved. Meanwhile, the method has a high haploid induction rate for preparing maize haploid. The application has important application value.

The invention discloses a corn bract character regulation gene ZmE3 and application thereof, and relates to the technical field of molecular biology and genetic engineering. The invention successfully clones and verifies the function of a corn bract character key regulatory gene ZmE3. By creating an overexpression strain and a knockout strain of the transgenic maize, the invention finds that the expression level of the ZmE3 gene is in significant positive correlation with the width, length, thickness and dry weight of bracts. Specifically, the gene is knocked out, so that the bracts are obviously narrowed, shortened and thinned, and the biomass accumulation is reduced; overexpression of the gene produces an opposite gain effect. More importantly, the gene indirectly affects the dehydration process of corn kernels by regulating and controlling the physical characters of bracts. The ZmE3 gene provides a new perspective for analyzing a bract development molecular mechanism, and provides an important technical tool and theoretical support for corn molecular breeding meeting diversified production targets.

A novel transgenic corn elite event designated MZIR098 is disclosed. The invention relates to DNA sequences of the recombinant constructs inserted into the corn genome and of genomic sequences flanking the insertion site that resulted in elite event MZIR098. The invention further relates to assays for detecting the presence of the DNA sequences of corn elite event MZIR098, to corn plants and corn seeds comprising the genotype thereof, and to methods for producing a corn plant by crossing a corn plant comprising the elite event MZIR098 genotype with itself or another corn variety.

The present invention relates to a transgenic Zea mays L. event KA-1G2Ø15-1 and a related nucleic acid sequence, a detection method, a Zea mays L. plant, and a cultivation method. The Zea mays L. plant has herbicide tolerance.

The invention discloses application of ZmCCT protein and related biological materials thereof in regulation and control of lodging resistance of corn. According to the invention, ZmCCT transgenic corn is firstly constructed, and the lodging resistance of the ZmCCT transgenic corn is analyzed to find that overexpression of ZmCCT can improve the lodging resistance of corn. In order to verify the function of the ZmCCT, ZmCCT knockout corn is also constructed, and the lodging resistance of the corn is analyzed to find that the lodging resistance of the corn can be reduced by knocking out the ZmCCT. In addition, it is found that ZmCCT positively regulates and controls the corn overground part stalk lodging resistance, and the resistance of corn to underground part root lodging can be improved by influencing the root system configuration. It is found for the first time that ZmCCT protein and related biological materials participate in regulation and control of the lodging resistance of corn, and the ZmCCT protein plays an important role in cultivation of lodging-resistant corn varieties.

The invention discloses an application of a transcription factor MYB39 (Th4) in improving plant biomass, overexpression of the transcription factor MYB39 gene in corn can effectively improve the plant biomass and nitrogen utilization efficiency, and when transgenic corn is subjected to low-nitrogen treatment, the overground part fresh weight is improved by 31.5%, the underground part fresh weight is improved by 15.9%, and the total biomass is improved by 23.9%; during high nitrogen treatment, the fresh weight of the overground part is increased by 18.2%, the fresh weight of the underground part is increased by 2.2%, and the total biomass is increased by 11.3%, which indicates that the MYB39 gene has the effect of increasing the biomass of the corn plant and can be used for cultivating high-biomass plant varieties with high nitrogen efficiency.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of corn casein kinase ZmCK2 beta or a coding gene thereof in regulation and control of corn resistance to corn yellow mosaic virus. The host protein ZmCK2 beta interacting with the CP is obtained through screening, it is found that ZmCK2 beta and ZmCK2 alpha can interact inside and outside a plant body, and CP and ZmCK2 alpha only interact in the plant body; the ZmCK2 beta and the ZmCK2 alpha-ZmCK2 beta complex in the plants are respectively silenced, and after the MaYMV is inoculated, the virus accumulation amount on the silenced plants is reduced, which indicates that the silenced ZmCK2 beta and the silenced ZmCK2 alpha-ZmCK2 beta complex can inhibit infection; a ZmCK2 beta-knockout transgenic corn strain is constructed, and it is found that the virus accumulation amount on the plant is also reduced after ZmCK2 beta is knocked out. The invention provides a scientific basis for deeply analyzing the MaYMV pathogenic mechanism, and lays a theoretical foundation for formulating a virus prevention and control strategy.

The invention discloses a novel corn gene editor based on glycosidase and application of the novel corn gene editor, the gene editor realizes accurate base editing of corn genes, G-to-T base replacement, and / or G-to-A base replacement, and / or G-to-C base replacement, and the gene editor has high editing efficiency in corn protoplast and transgenic corn plants. The invention also provides a corn gene fixed-point editing method. The corn gene editor DNA based on glycosidase can realize directional base replacement in corn, is high in accuracy, provides an efficient tool for corn genome editing, fills the blank of corn glycosidase base editor, and can be widely applied to corn molecular breeding.

The application provides a method for improving the disease resistance of corn sheath blight, and belongs to the technical field of genetic engineering disease resistance, wherein the disease resistance of transgenic corn to sheath blight is greatly improved after overexpression of ZmLOX12 protein in corn plants, the disease resistance effect of the transgenic crops is greatly improved, and the problems of few disease resistance countermeasures for corn sheath blight in the prior art are solved; the gene is successfully made to have the characteristics of high sheath blight resistance by means of genetic engineering, and the breeding efficiency of the disease-resistant plants is improved at a certain level, and the breeding cost is reduced. ZmLOX12 The gene has the characteristics of high sheath blight resistance, and the breeding efficiency of the disease-resistant plants is improved at a certain level, and the breeding cost is reduced.

A modified corn event ZMBCS216090 is provided as well as plants, plant cells, seeds, plant parts, progeny plants, and commodity products comprising the modified corn event ZMBCS216090. Provided are polynucleotides and sequences that are specific for the modified corn event ZMBCS216090, methods for making the modified corn event ZMBCS216090, methods for making and using plants, plant cells, seeds, plant parts, progeny plants, and commodity products comprising the modified corn event ZMBCS216090, and methods for making and using the plants, plant cells, seeds, plant parts, progeny plants, and commodity products comprising the modified corn event ZMBCS216090. And a method for detecting the modified corn event ZMBC216090 or a polynucleotide or a DNA sequence that is specific for the modified corn event ZMBC216090 in a DNA molecule or a sample.

The invention discloses a DNA (Deoxyribose Nucleic Acid) molecule of a corn transformant ZD818 and a detection method. The transgenic corn event ZD818 is obtained by inserting an exogenous T-DNA (Transgenic Deoxyribose Nucleic Acid) containing insect-resistant genes cry2Ab and cry1Da and a herbicide-resistant gene cp4epsps into a specific site of a fifth chromosome of a corn genome; a nucleic acid sequence for specifically detecting the event comprises SEQ ID NO.1 or a complementary sequence thereof, and / or SEQ ID NO.2 or a complementary sequence thereof. Plants of the transgenic maize event ZD818 have high resistance to lepidoptera pests and high tolerance to glyphosate herbicides. The event-specific DNA detection method provided by the invention can accurately and stably identify the ZD818 event through nucleic acid amplification, and is of great significance to intellectual property protection, breeding application and safety supervision of the transgenic maize.

The application discloses a method for producing human serum albumin by using corn seeds, and the method comprises the following steps: introducing a human serum albumin gene into corn by a DNA recombination technology, obtaining corn with high expression of human serum albumin, and efficiently purifying human serum albumin in the corn seeds, so as to produce human serum albumin more efficiently and at a lower cost. On a unit area, more human serum albumin can be produced by planting the transgenic corn. The application discloses, for the first time, that human serum albumin is expressed by using corn seeds through the DNA recombination technology, wherein the expression level of the high-expression transformant is at least 1% of the weight of the corn seeds, that is, the yield of human serum albumin per kilogram of corn seeds is more than 10 grams. The transgenic corn obtained by using the application can produce human serum albumin more efficiently and at a lower cost: the photosynthesis efficiency of the corn is relatively high, the yield per unit area is significantly higher than that of rice, and the yield of human serum albumin of the high-expression corn transformant is higher than that of the rice transformant.