147 results about "Genetically modified maize" patented technology

The invention belongs to the technical field of genetic engineering, and particularly relates to a method for creating a corn dwarfing material and application of the corn dwarfing material. The invention discloses a method for creating a corn dwarfing material, which specifically comprises the following steps: carrying out specific key site editing on a target gene ZmGA20ox3 and / or ZmGA20ox5 in corn by using a genetic engineering means, and carrying out specific amino acid site mutation on a protein coded by the target gene ZmGA20ox3 and / or ZmGA20ox5 subjected to specific key site editing, and further cultivating and screening to obtain the corn dwarfing material. The method provided by the invention can be used for cultivating transgenic corn plants with different plant heights and other agronomic traits, and meanwhile, a new breeding technical scheme is provided for creating dwarf corn, reducing the height of a corn ear, reducing the included angle of corn plant leaves and increasing the corn yield; crops can be more densely planted in agriculture, so that the yield of the crops in unit area is improved, and the method has important breeding significance.

The invention provides a homozygosity and heterozygosity identification method for insect-resistant and herbicide-resistant transgenic maize LD05. The method can accurately distinguish LD05 homozygote from LD05 heterozygote so as to quickly obtain a pure line, effectively shorten the time for introducing excellent characters into a conventional parent selfing line and accelerate the biological breeding process.

The invention discloses an effect protein E56, a coding gene and application of the effect protein E56 to improvement of southern rust resistance of corn, and belongs to the technical field of agricultural biology. The amino acid sequence of the effector protein E56 is as shown in SEQ ID NO. 1. A reverse genetics method is utilized to analyze the Puccinia polystachys effect protein E56 gene, and the E56 gene is up-regulated in expression in the Puccinia polystachys infection process. The E56 gene is silenced by adopting a host-mediated gene silencing technology, and the toxic function of the E56 gene in the infection process of the southern rust disease is determined. A specific fragment of the E56 gene is cloned to an RNAi interference vector, a corn immature embryo is transformed by using an agrobacterium tumefaciens-mediated transgenic technology, and an obtained transgenic corn plant shows resistance to the corn southern rust disease. The E56-RNAi transgenic plant shows resistance to puccinia polypoda, so that the effect protein E56 can be used for creating a new strain for resisting the southern rust disease.

The invention relates to a corn transformation event MN85N-E6 nucleic acid molecule and a detection method. The corn transformation event MN85N-E6 nucleic acid molecule comprises a sequence as shown in SEQ ID NO: 1 or a reverse complementary sequence thereof, or a sequence as shown in SEQ ID NO: 2 or a reverse complementary sequence thereof. The corn transformation event MN85N-E6 provided by the invention has the characteristics of insect resistance and glufosinate-ammonium herbicide resistance, and the detection method can accurately and quickly identify whether a biological sample contains DNA molecules of the transgenic corn event MN85N-E6 or not.

The present invention provides a nucleic acid sequence, which comprises one or more selected from the sequences consisting of SEQ ID NOs: 1-7 and the complementary sequences thereof, the nucleic acid sequence is derived from plants, seeds or cells comprising the transgenic maize event LP026-2, and the representative sample of the seeds comprising the event having been deposited under deposit number CCTCC NO: P202207.

The invention provides a transgenic corn event MON95275, plants, plant cells, seeds, plant parts (including pollen, seed, and cells, and tissues corresponding to tassel, root, stalk, stem, leaf, cobb, and the like), progeny plants, commodity products comprising detectable amounts of corn event MON95275 DNA. The invention also provides polynucleotides specific for corn event MON95275 and methods for using and detecting corn event MON95275 DNA as well as plants, plant cells, seeds, plant parts, progeny plants, and commodity products comprising corn event MON95275. The invention also provides methods related to making and using corn event MON95275.

The invention belongs to the technical field of plant breeding, and relates to a transgenic maize event LP112-1 and a detection method thereof. The invention provides a nucleic acid molecule for detecting a transgenic maize event LP112-1, the sequence of the nucleic acid molecule is as shown in SEQ ID NO: 1-2, SEQ ID NO: 3-4, SEQ ID NO: 5 or one or more groups of complementary sequences thereof, the nucleic acid molecule is derived from the transgenic maize event LP112-1, and the sequence of the nucleic acid molecule is as shown in SEQ ID NO: 1-2, SEQ ID NO: 3-4, SEQ ID NO: 5. The corn seed containing the transgenic corn event LP112-1 has been preserved in the China Center for Type Culture Collection (CCTCC) with the preservation number of CCTCC NO: P202432. Maize plants comprising the transgenic maize event LP112-1 of the present invention have the following advantages: free of economic losses due to lepidoptera pests; an agricultural herbicide containing glyphosate can be applied to corn crops; the corn yield is not reduced; and the breeding efficiency is enhanced, and the molecular marker can be used for tracking transgenic insertion fragments in a breeding population and a progeny thereof.

The invention provides a primer pair, a kit, a PCR (Polymerase Chain Reaction) detection method and application of the PCR detection method in detection of transgenic maize LD05.

The invention provides an application of a corn disease-resistant gene ZmCRK10 and a disease-resistant molecular marker thereof in improvement of corn stem rot resistance, and belongs to the technical field of plant genetic engineering. The invention provides application of a disease-resistant gene ZmCRK10 or a disease-resistant protein ZmCRK10-T03 in improvement of corn stem rot resistance. The disease-resistant gene ZmCRK10 or the disease-resistant protein ZmCRK10-T03 can be used for constructing a transgenic corn variety resistant to corn stem rot. The invention further provides application of the disease-resistant molecular marker ZmCRK10TypeB of the corn disease-resistant gene ZmCRK10 in improving the corn stem rot resistance, and effective auxiliary molecular marker selection can be provided for corn stem rot resistance breeding.

The invention relates to a nucleic acid molecule for detecting a transgenic maize event DBN9641 containing a universal hybrid seed production (UHT) and a detection method of the nucleic acid molecule, and the nucleic acid molecule comprises SEQ ID NO: 1 or a complementary sequence of the SEQ ID NO: 1, and SEQ ID NO: 2 or a complementary sequence of the SEQ ID NO: 2. The corn plant DBN9641 is endowed with a function of keeping sterility of a sterile line in a hybrid seed production technology, so that a castration step in a hybrid seed production process is omitted, manpower and material resources are saved, the operation is simple, and the detection method can accurately and quickly identify whether a biological sample contains DNA molecules of the transgenic corn event DBN9641 or not.

The invention relates to a corn transformation event MN85zN-Ex nucleic acid molecule and a detection method. The corn transformation event MN85zN-Ex nucleic acid molecule comprises a sequence shown in SEQ ID NO: 1 or a reverse complementary sequence thereof, or a sequence shown in SEQ ID NO: 2 or a reverse complementary sequence thereof. The corn transformation event MN85zN-Ex has the characteristics of insect resistance and glufosinate-ammonium herbicide resistance, and the detection method can accurately and quickly identify whether a biological sample contains the DNA molecule of the transgenic corn event MN85zN-Ex or not.

The invention belongs to the technical field of biology, and relates to a synthesized gene tandem expression cassette and application thereof. According to the invention, the genes cry1Ab, vip3Aa, gr79-epsps and gat are combined into a gene tandem expression cassette form and are integrated into a corn material, so that compared with a single-gene insect-resistant or herbicide-resistant gene adopted in the prior art, the resistance of transgenic corn to lepidoptera pests is remarkably improved, the tolerance to glyphosate also reaches a high resistance level, and the application prospect is wide. Especially, the insect-resistant genes have a synergistic effect, and the effect of quickly killing insects is achieved.

Provided is transgenic maize event QY2569-42, and further provided are a nucleic acid sequence and detection method for detecting maize plant QY2569-42. The nucleic acid sequence of the maize plant comprises SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 11 and / or SEQ ID NO: 12 or a nucleic acid sequence complementary thereto. The maize plant QY2569-42 has good resistance to Lepidopteran insects and good tolerance to glufosinate ammonium herbicides, and has no effect on the yield; and the detection method can accurately and rapidly identify whether the DNA molecule of transgenic maize plant QY2569-42 is contained in a biological sample.

The invention discloses a primer group for homozygosity and heterozygosity identification of transgenic maize CC-2 and a detection method. The invention provides a primer pair group. The primer pair group consists of a primer pair 1 (SEQ ID No.1 and SEQ ID No.2) and a primer pair 2 (SEQ ID No.3 and SEQ ID No.2), the primer pair 1 is used for specifically detecting whether to-be-detected corn is transgenic corn CC-2 or not; the primer pair 2 is used for detecting whether the insertion of an exogenous target gene maroACC in the transgenic maize CC-2 is heterozygous or homozygous. Two pairs of primers are designed for the transgenic maize CC-2, a set of PCR detection method for homozygosis and heterozygosis of the transgenic maize CC-2 is established, and the PCR detection method is of great significance to screening of homozygous transformants and improvement of seed purity and quality in the breeding process.

The invention discloses a transgenic corn insect-resistant effect evaluation method, which comprises the following steps: constructing a standardized planting system, adopting a special powder-flocculent feed to realize unified inoculation of multiple insects, standardizing survey indexes and calculation logic, and finishing data processing and insect-resistant grade judgment in combination with a Python automatic analysis system. According to the method, a complete evaluation system of standardized planting, unified insect inoculation, unified investigation and automatic analysis is formed, the insect inoculation efficiency of the method is improved by 17 times compared with that of a traditional method, the data processing efficiency of the method is improved by 1620 times compared with that of an Excel + SPSS combination, standardized technical support is provided for safety evaluation, variety certification and industrial popularization of transgenic maize, and the method has important practical application value.

The invention belongs to the technical field of herbicides, and particularly relates to a herbicide for preventing and removing weeds in transgenic corn and soybean fields. A herbicide for preventing and removing weeds in transgenic corn and soybean fields comprises an active component and an auxiliary component, and the active component is formed by compounding glyphosate or glufosinate-ammonium and Epyrifenacil according to the mass ratio of (1-80): (80-1). After glyphosate or glufosinate-ammonium is compounded with Epyrifenacil, the control effect on eleusine indica, abutilon fiber and the like can be improved, and the herbicide controlling spectrum is expanded. Besides, the herbicide active component is formed by compounding two weeding active components according to a certain proportion, compared with a single-action-mechanism herbicide, the herbicide resistance evolution of weeds can be effectively controlled, the service life of the herbicide can be prolonged, and then important support is provided for scientific and effective prevention and control of the weeds.

The invention discloses a primer probe combination and a kit for on-site detection of a transgenic maize transformant, and relates to the technical field of transgenic detection. The invention provides a primer probe combination for on-site detection of a transgenic maize transformant. The primer probe combination comprises a primer pair and a probe for detecting a transgenic maize strain MON810, the primer pair and the probe are used for detecting the transgenic maize strain TC1507; the primer pair and the probe are used for detecting the transgenic maize strain Bt11; the invention discloses a primer pair and a probe for detecting corn internal reference zSSIIb. The method for detecting the transgenic maize by adopting the primer probe combination and the kit disclosed by the invention integrates sample pretreatment, nucleic acid extraction, purification, amplification and detection, has better sensitivity and accuracy, is simple, convenient, rapid and time-saving to operate, and plays an important role in on-site rapid detection of transgenic maize research and safety management.

The present invention discloses a method for increasing the yield of maize. The method is to overexpress an auxin influx carrier protein in maize, screen for transformed plants with significantly increased ear row number, grain weight per ear, or test weight, and increase the yield of maize. Overexpression of the auxin transport protein in the present invention can significantly increase the test weight and ear row number of maize kernels in inbred lines and hybrids, thereby significantly increasing the yield. Under the same genetic background, both the test weight and ear row number of kernels are positively correlated with the yield of maize. Selecting transgenic maize into which the auxin transport protein gene has been introduced using the test weight and ear row number can effectively obtain high-yield transgenic maize.

The invention discloses a primer group for homozygosity and heterozygosity identification of transgenic maize ND207 and a detection method. The invention provides a primer pair group. The primer pair group consists of a primer pair 1 (SEQ ID No.1 and SEQ ID No.2) and a primer pair 2 (SEQ ID No.3 and SEQ ID No.4); the primer pair 1 is used for specifically detecting whether to-be-detected corn is transgenic corn ND207 or not; the primer pair 2 is used for detecting whether the insertion of an exogenous target gene in the transgenic maize ND207 is heterozygous or homozygous. Two pairs of primers are designed for the transgenic maize ND207, a set of PCR detection method for homozygosis and heterozygosis of the transgenic maize ND207 is established, and the PCR detection method is of great significance to screening of homozygous transformants and improvement of seed purity and quality in the breeding process.

The invention relates to the field of transgenic plants, in particular to a nucleic acid molecule, a primer group and application thereof. The present invention provides a nucleic acid molecule having a nucleotide sequence as any of SEQ ID NO: 1 to SEQ ID NO: 7. The invention provides a left flanking sequence and a right flanking sequence of an exogenous insertion vector of an insect-resistant and herbicide-resistant corn WYN8158 transformation event and application of the left flanking sequence and the right flanking sequence in detection of transgenic corn, and can be used for rapidly, efficiently and specifically detecting whether a sample to be detected is derived from a transgenic component or not. Meanwhile, technical support is provided for market identification and identification after commercialization and industrialization of the product, and meanwhile the method has important significance and wide prospects for promoting corn germplasm resource identification in China.

The invention discloses an insect-resistant and herbicide-resistant transgenic maize event JZL22k-1 and a detection method of the insect-resistant and herbicide-resistant transgenic maize event JZL22k-1. The transgenic maize event JZL22k-1 nucleic acid sequence comprises SEQ ID NO: 1 or a complementary sequence thereof, and / or SEQ ID NO: 2 or a complementary sequence thereof; the transgenic maize plant JZL22k-1 has good resistance to lepidoptera insects and good tolerance to glyphosate herbicides and has no influence on the yield, and the detection method can accurately and stably identify the existence of a JZL22k-1 transgenic event by applying a nucleic acid amplification method. The method is of great significance in implementation of research of JZL22k-1, traceability of production processing and application and whole-process supervision.

This invention discloses the application of LNA1 protein and related biological materials in regulating the number of leaves on the ear and yield of maize. The invention first involves overexpression of LNA1 protein in wild-type maize. lna1 , received lna1 Gene-modified maize and statistical analysis of its leaf number revealed that overexpression... lna1 It can increase the number of leaves on the corn ear and the total number of leaves. To further verify the function of LNA1, this invention knocked out LNA1 in wild-type corn. lna1, get lna1 Knockout corn and statistical analysis of its leaf number and plant height revealed that: lna1 It can reduce the number of leaves on the corn ear and the plant height. Furthermore, this invention has demonstrated improved efficiency by constructing NIL-Popcorn and NIL-MS71. lna1 Gene expression levels can increase maize yield. These results indicate that the LNA1 protein and related biological materials can regulate leaf number, yield, and plant height, and will play an important role in breeding high-yielding maize varieties.

The present invention belongs to the field of molecular biology technology, and specifically relates to a transgenic corn event LP1141-1 and a detection method thereof. The present invention provides a nucleic acid sequence for detecting transgenic corn event LP1141-1, wherein the nucleic acid sequence is selected from one or more of the sequences SEQ ID NO: 1-7 or their complementary sequences, and the nucleic acid sequence is derived from transgenic corn event LP1141-1. Representative samples of seeds of the transgenic corn event LP1141-2 have been deposited in the China Center for Type Culture Collection on April 13, 2025, with a deposit number of CCTCC NO: P202511. The transgenic corn event LP1141-1 of the present invention has the triple traits of obtaining red maintainer seeds, breeding sterile materials, and glufosinate-ammonium herbicide resistance. The event simplifies the screening method and saves labor costs; does not affect corn yield; and can track transgenic inserts in the breeding population and its progeny using molecular markers. The detection method provided by the present invention can quickly, accurately, and stably identify the presence of plant material derived from the transgenic corn event LP1141-1.

The invention relates to the field of transgenic plants, in particular to a nucleic acid molecule, a primer group and application thereof. The present invention provides a nucleic acid molecule having a nucleotide sequence as any of SEQ ID NO: 1 to SEQ ID NO: 7. The invention provides a left flanking sequence and a right flanking sequence of an exogenous insertion vector of an insect-resistant and herbicide-resistant corn WYN8158 transformation event and application of the left flanking sequence and the right flanking sequence in detection of transgenic corn, and can be used for rapidly, efficiently and specifically detecting whether a sample to be detected is derived from a transgenic component or not. Meanwhile, technical support is provided for market identification and identification after commercialization and industrialization of the product, and meanwhile the method has important significance and wide prospects for promoting corn germplasm resource identification in China.

The invention belongs to the technical field of molecular biological detection, and particularly relates to a primer group and a method for detecting homozygote and heterozygote of transgenic maize LP026-2. The homozygote and heterozygote of the transgenic maize LP026-2 are distinguished by adopting a KASP (Competitive Allele Polymerase Chain Reaction) technology. According to the reaction, two possible genotypes of a detection sample in each reaction hole are subjected to two-color fluorescence reaction to reflect whether the genotype of the transgenic maize LP026-2 is a wild type, a homozygote or a heterozygote. The method has the advantages that high-throughput and high-sensitivity genotyping is realized, the method is suitable for LP026-2 derived varieties with different genetic backgrounds, the foreground selection requirements of large-scale samples in the backcross transformation process are met, and a technical template is provided for detection of other transgenic crops.

The invention belongs to the technical field of plant breeding, and relates to a transgenic maize event LP111-2 and a detection method thereof. The invention provides a nucleic acid molecule for detecting a transgenic maize event LP111-2, the sequence of the nucleic acid molecule is as shown in SEQ ID NO: 1-2, SEQ ID NO: 3-4, SEQ ID NO: 5 or one or more groups of complementary sequences thereof, the nucleic acid molecule is derived from the transgenic maize event LP111-2, and the sequence of the nucleic acid molecule is as shown in SEQ ID NO: 1-2, SEQ ID NO: 3-4, SEQ ID NO: 5 or one or more groups of complementary sequences thereof. Maize seeds comprising the transgenic maize event LP111-2 have been preserved in the China Center for Type Culture Collection (CCTCC) with a preservation number of CCTCC NO: P202430. Maize plants comprising the transgenic maize event LP111-2 of the present invention have the following advantages: free of economic losses due to lepidoptera pests; an agricultural herbicide containing glyphosate can be applied to corn crops; the corn yield is not reduced; and the breeding efficiency is enhanced, and the molecular marker can be used for tracking transgenic insertion fragments in a breeding population and a progeny thereof.

The application discloses a kind of evaluation Spodoptera frugiperda to corn ear damage method, belong to harmful insect ecological control technical field.The application first proposes a kind of new method by measuring corn ear bending angle to judge the damage degree of Spodoptera frugiperda to corn ear, specifically: corn milk ripening period, select the corn damaged by Spodoptera frugiperda, and measure corn ear bending angle, according to corn ear bending angle, the damaged condition of corn yield is evaluated.The application can not only efficiently, quickly and objectively judge the damage degree of corn ear, but also solve the problems of high difficulty in area measurement and large error caused by irregular damaged parts of corn ear in the existing evaluation method, provide a scientific basis for transgenic corn insect resistance evaluation, and have important significance for improving Spodoptera frugiperda control technology system.

The invention relates to an exogenous insertion sequence of a glyphosate and glufosinate-ammonium resistant corn transformation event LE4, a detection method and application. A nucleic acid molecule sequence of the corn LE4 comprises SEQ ID NO.1 or SEQ ID NO.2 or SEQ ID NO.13 or a reverse complementary sequence of the SEQ ID NO.1 or SEQ ID NO.2 or SEQ ID NO.13. The invention further relates to a method for detecting the glyphosate and glufosinate-ammonium resistant corn transformation event LE4. The corn transformation event LE4 has glyphosate and glufosinate herbicide resistance and excellent agronomic traits, and the detection method can accurately and quickly identify whether a biological sample contains DNA molecules of the transgenic corn event LE4 or not.

Modified corn event MON95275 and plants, plant cells, seeds, plant parts, progeny plants, commodity products comprising detectable amounts of a modified corn event MON95275 DNA are provided. Polynucleotides and sequences specific for a modified corn event MON95275, methods for making a modified corn event MON95275, methods for making and using plants, plant cells, seeds, plant parts, progeny plants, and commodity products comprising a modified corn event MON95275, and methods for detecting a modified corn event MON95275, and polynucleotides or DNA sequences specific for a modified corn event MON95275, in a DNA molecule or sample, are provided.