19 results about "Zea mays" patented technology

The presently disclosed subject matter relates to the use of a haploid inducing line transformed such that it contains DNA encoding a cellular mechanism capable of editing a gene. This mechanism is preferentially expressed in zygotic cells. Exemplary promoters include, but are not limited to, a VSP promoter, an RZDP promoter, an SCE1 promoter, and a U3 promoter. The promoters can be obtained from arabidopsis thaliana, corn, soybean, wheat, sunflower, rice and tomato.

The invention relates to a process for dehydrating whole kernels of Zea mays L. (maize), which comprises: providing clean, nixtamilised whole maize kernels; boiling the maize kernels in 4 parts water to 1 part maize kernels at 94ºC for 210-300 mins; rinsing the boiled maize kernels with clean water; freezing the boiled whole kernels at between -30 and -40ºC for 10-12 h; applying vacuum to the maize kernels, maintaining freezing at between -28 and -33ºC at a pressure below 80 Pa for 1-2 h; dehydrating the maize kernels, maintaining freezing at between -28 and -33ºC at a pressure of 23-26 Pa for 10-14 h; and continuing to dehydrate the maize kernels by increasing the temperature to 48-53ºC at 23-26 Pa for 2-4 h.

This invention discloses a traditional Chinese medicine formula for preventing and treating feline urinary tract diseases, its preparation method, and its application. The formula consists of the following components in parts by weight: 15-25 parts of Psoralea corylifolia, 15-25 parts of Alpinia oxyphylla, 10-20 parts of Trigonella foenum-graecum, 5-15 parts of Zea mays, 5-15 parts of Juncus effusus, 10-20 parts of Dioscorea hypoglauca, 10-20 parts of Phellodendron chinense, 10-20 parts of Lonicera japonica, 5-15 parts of Paeonia lactiflora, and 5-15 parts of Glycyrrhiza uralensis. The traditional Chinese medicine formula provided by this invention has diuretic and urinary tract mucosal repairing effects. It can effectively relieve symptoms such as urinary stones, painful urination, hematuria, or gravel in the urine caused by damp-heat in the lower abdomen. It promotes the repair of the urinary tract mucosa in animals with urinary tract diseases, alleviates inflammatory responses, and prevents the formation of crystals in the urine.

The present invention relates to antiperspirant and deodorant compositions for correction of body odour, and more particularly relates to antiperspirant and deodorant compositions for human hygiene. The disclosed anti-perspirant and deodorant composition comprises isobutane, propane, butane, cyclopentasiloxane, caprylic / capric triglyceride, stearalkonium hectorite, propylene carbonate, c12-15 alkyl benzoate, parfum, silver lactate, lactic acid, aqua, zea mays starch, menthyl ethylamido oxalate, argania spinosa kernel oil. Furthermore, the active antimicrobial silver ingredient is dermatologically tested and is capable of diminishing odor-causing bacteria in addition to prevention of irritation and itching.

The application provides a SNP molecular marker combination for whole genome genotyping of explosive corn, a detection chip and application, and relates to the technical field of molecular breeding.The application provides a SNP molecular marker combination for whole genome genotyping of explosive corn, and the genotyping object comprises 6419 SNP sites;the SNP markers in the application uniformly cover the whole corn reference genome, and can provide high-quality molecular markers for map-based cloning of explosive corn specific agronomic trait control genes.Meanwhile, the application can also perform genotyping on different types of corn.The explosive corn germplasm resource is narrower than other types of corn germplasm resources, and the chip of the application can make up for the low diversity of the chip based on the diversity of food corn in explosive corn genotyping.

Provided are a southern corn leaf blight-resistant Thph2 protein from Trichoderma harzianum, a ZmGLP1-17 protein from Zea mays L., and a method for preparing transgenic Zea mays L. Further provided are a cellobiohydrolase protein Thph2 from Trichoderma harzianum T30, a coding gene for the cellobiohydrolase protein, and use of the cellobiohydrolase protein in inducing a response of a GLP ZmGLP1-17 from Zea mays L. against southern corn leaf blight. Trichoderma harzianum strain OEthph2 overexpressing a Thph2 gene is constructed by a transgenic technique. OEthph2 may induce the expression of the GLP ZmGLP1-17 in roots of Zea mays L. and enhance the resistance of leaves of Zea mays L. to the infection of Cochliobolus heterostrophus. ZmGLP1-17 transgenic Zea mays L. material is constructed. The OEthph2 engineered strain may be combined with the ZmGLP1-17 transgenic Zea mays L. material to synergistically improve a control effect for southern corn leaf blight.

The present invention relates to a transgenic Zea mays L. event KA-1G2Ø15-1 and a related nucleic acid sequence, a detection method, a Zea mays L. plant, and a cultivation method. The Zea mays L. plant has herbicide tolerance.

This invention discloses a compound stone granule for treating the urinary system, its preparation method, and its application, belonging to the field of traditional Chinese medicine preparation technology. The granules are made from Lysimachia christinae, Lygodium japonicum, Gallus gallus domesticus gizzard lining, Pyrrosia lingua, Kochia scoparia, Polygonum capitatum, Plantago asiatica, Gentiana scabra, Polygonum cuspidatum, Lysimachia christinae, Sedum sarmentosum, Zea mays silk, Epimedium brevicornu, Juncus effusus, Achyranthes bidentata, and Glycyrrhiza uralensis. Preparation involves reflux extraction with 60% ethanol three times, 2 hours each time, followed by vacuum concentration to obtain a compound stone extract. The extract is then wet-granulated with lactose at a 1:3 ratio, and dried to obtain the final product. The process of this invention has been optimized through orthogonal experiments, resulting in a stable extract yield. The particle size, moisture content, and solubility of the granules all meet the pharmacopoeia standards. A TLC-HPLC quality control method has been established. In vitro experiments have confirmed that the aqueous solution of this compound stone granules has a concentration-dependent inhibitory effect on calcium oxalate crystallization, with a high concentration inhibition rate of 39.773%. It can protect renal tubules and optimize the urinary tract microenvironment, making it suitable for the prevention and treatment of urinary system stones. The process is simple and low-cost, making it suitable for industrial production.

The invention belongs to the technical field of traditional Chinese medicines, mainly relates to a medicinal and edible cream formula and a preparation method thereof, and in particular relates to a Shuanqu cream for clearing damp and reducing phlegm, burning fat and reducing weight and improving edema and a preparation method thereof. The ointment comprises the following medicinal and edible medicinal materials in parts by weight: 10-12 parts of platycodon grandiflorum, 9-12 parts of pericarpium citri reticulatae, 15-20 parts of lotus leaves, 7-10 parts of agastache rugosus, 13-15 parts of poria cocos, 10-13 parts of exocarpium citri rubrum, 7-10 parts of amomum tsao-ko and 14-19 parts of corn stigma. Wherein the agastache rugosus and the amomum tsao-ko are subjected to supercritical CO2 extraction, and the platycodon grandiflorum, the pericarpium citri reticulatae, the poria cocos, the lotus leaves and the corn stigma are fermented The compound bacterial strain is prepared from bacillus coagulans TS346726 and kluyveromyces marxianus ATCC-36534, and the bacillus coagulans TS346726 and the kluyveromyces The prepared ointment has the effects of clearing damp, reducing phlegm, burning fat, reducing weight and improving edema.

The present invention relates, inter alia, to vegetative plant parts, such as from a Sorghum sp. and / or a Zea mays plant, which comprise a total fatty acid (TFA) content which comprises fatty acids esterified in the form of triacylglycerols (TAG) and fatty acids in the form of lipids other than TAG, wherein the vegetative plant parts comprise greatly increased levels of TFA, for example a TFA content of about 5% (w / w dry weight). The present invention also relates to the use of the vegetative plant parts as a feedstuff, and / or to produce a feedstuff, for animal consumption.

The invention provides a method for analyzing the action mechanism of wind-dispelling five-herb decoction for treating gout based on network pharmacology and molecular docking and application, and relates to the technical field of traditional Chinese medicine information analysis, the method comprises the following steps: acquiring a common target spot of a drug target spot of the wind-dispelling five-herb decoction and a gout disease target spot; the wind-dispelling five-herb decoction comprises lysimachia christinae hance, oldenlandia diffusa, lalang grass rhizome, plantain herb Constructing a protein interaction network based on the common target; screening core targets in the protein interaction network, and identifying core active components of the Chinese herbal medicine prescription; performing pathway enrichment analysis and function enrichment analysis on the common target spot to determine a drug action mechanism; and verifying the interaction strength of the core active component and the core target spot through molecular docking. By directly calling a plurality of biological databases and integrating molecular docking verification, the analysis process is simplified, and the mechanism that the wind-dispelling five-herb decoction acts on key targets and signal pathways through core active components to treat gout is accurately disclosed.

The present application relates to the technical field of molecular biology, and particularly relates to a flanking sequence of an exogenous inserted fragment of a pest-resistant transgenic corn AM11 and application thereof. The flanking sequence is a 5' end flanking sequence of the exogenous inserted fragment, specifically a sequence as shown in SEQ ID NO. 9 or a specific fragment thereof; or a 3' end flanking sequence of the exogenous inserted fragment, specifically a sequence as shown in SEQ ID NO. 10 or a specific fragment thereof. The flanking sequence is derived from a pest-resistant transgenic corn event AM11. The corn seed of the pest-resistant transgenic corn AM11 has been preserved in the China General Microbiological Culture Collection Center on September 5, 2025, with a preservation number of CGMCC NO. 46691 and a classification name of Zea mays L. Zea mays The flanking sequence of the present application can effectively determine whether the pest-resistant transgenic corn event AM11 is successfully obtained, thereby providing a strong guarantee for monitoring the pest-resistant transgenic corn AM11 and its offspring.

The application relates to the technical field of rice planting methods, in particular to a nutrient solution for cultivating Xiang He Nu 'black He', and application and cultivation method thereof. The application can use the common rice nutrient solution added with exogenous amino acids, added with exogenous organic acids, and added with the common rice nutrient solution with a compound treatment of a final concentration of 0.4 mM phenylalanine and 5 mu M salicylic acid for 'black He'. The nutrient solution for cultivating Xiang He Nu 'black He' can be applied to improve the quality of rice, especially Xiang He Nu 'black He'. The nutrient solution for cultivating Xiang He Nu 'black He' can effectively improve the plant height, biomass (fresh weight), nitrogen content, chlorophyll content and single plant dry weight of 'black He'. The problem of poor growth and development of 'black He' in the common nutrient solution can be solved, the adaptability of 'black He' is enhanced to a certain extent, and the yield is slightly improved, which will be beneficial to the process of building green agriculture.

The invention belongs to the technical field of traditional Chinese medicine compositions, and particularly relates to a traditional Chinese medicine composition for treating upper digestion type diabetes and a preparation method and application thereof. The traditional Chinese medicine composition is prepared from the following raw materials in parts by mass: 25 to 35 parts of herba eupatorii, 25 to 35 parts of radix codonopsis, 25 to 35 parts of radix rehmanniae recen, 25 to 35 parts of radix puerariae, 25 to 35 parts of radix trichosanthis, 20 to 30 parts of radix ophiopogonis, 15 to 25 parts of rhizoma coptidis, 15 to 25 parts of radix adenophorae, 15 to 25 parts of rhizoma anemarrhenae, 15 to 25 parts of rhizoma polygonati, 15 to 25 parts of poria with hostwood, 15 to 25 parts of corn stigma, 15 to 25 parts of folium mori, 5 to 15 parts of fructus schizandrae and 5 to 10 parts of liquorice root. The traditional Chinese medicine composition provided by the invention can be used for effectively treating the upper-digestion diabetes mellitus on the basis of the main pathogenesis of the upper-digestion diabetes mellitus according to medication experience and traditional Chinese medicine theories.

This invention relates to the field of biotechnology, specifically disclosing the Zm00001d021666 gene, which regulates the secretion of organic acids in maize under alkaline stress, and its application in maize resistance breeding. The nucleotide sequence of this gene is shown in SEQ ID No. 1, and the amino acid sequence of the encoded protein is shown in SEQ ID No. 2. The gene is located on chromosome 7 of maize, between 159493892 and 159495965 (reference genome: Zea mays RefGen_V4), and encodes a protein with isocitrate lyase activity. This invention is the first to identify and confirm that this gene is associated with the maize alkaline stress response. Functional verification shows that upregulating the expression of the Zm00001d021666 gene can significantly increase the secretion of organic acids in maize under alkaline stress, improve the alkaline environment of the rhizosphere soil, and enhance alkalinity tolerance. This invention also relates to a recombinant expression vector containing this gene and its application in breeding alkaline-tolerant transgenic maize. This invention provides key gene resources and technical support for improving maize's adaptability to alkali stress and cultivating new high-yield maize varieties suitable for planting in saline-alkali land, and has important theoretical and applied value.

The invention is applicable to the field of molecular biology and biotechnology, and provides a corn vacuolar H < + >-pyrophosphatase 1 gene ZmVPP1 and application thereof. The nucleotide sequence of the corn vacuole type H < + >-pyrophosphatase 1 gene ZmVPP1 is as shown in SEQ ID NO: 1; the corn vacuole type H < + >-pyrophosphatase 1 is coded by a ZmVPP1 gene, and the amino acid sequence of the corn vacuole type H < + >-pyrophosphatase 1 is shown as SEQ ID NO: 2. The gene is cloned by taking a corn inbred line B73 as a material, and after a plant expression vector is constructed, arabidopsis thaliana is transformed by an agrobacterium flower dipping method, so that a T3-generation transgenic arabidopsis thaliana strain with genetic stability is obtained. Saline-alkali stress treatment is carried out on transgenic arabidopsis thaliana and wild type arabidopsis thaliana, it is found that the corn vacuole type H < + >-pyrophosphatase 1 gene ZmVPP1 can effectively improve saline-alkali resistance of arabidopsis thaliana, and a key gene and technical support are provided for saline-alkali resistance improvement of plants.

This invention provides a traditional Chinese medicine composition for treating gallstones and its application. It comprises the following raw materials in parts by weight: Bupleurum chinense 6-12 parts, Scutellaria baicalensis 6-12 parts, Curcuma longa 2-5 parts, Artemisia capillaris 3-8 parts, Lysimachia christinae 2-6 parts, Coptis chinensis 4-8 parts, Anemarrhena asphodeloides 3-8 parts, Ophiopogon japonicus 3-8 parts, Citrus reticulata peel (processed with vinegar) 1-3 parts, Paeonia lactiflora 1-3 parts, Astragalus membranaceus 1-5 parts, Poria cocos 1-5 parts, Prunus persica kernel (processed with vinegar) 1-5 parts, Trichosanthes kirilowii peel 1-5 parts, Amomum villosum 1-5 parts, Pogostemon cablin 1-5 parts, Liquidambar formosana 1-5 parts, Luffa cylindrica 1-5 parts, Gleditsia sinensis thorns 1-5 parts, Coix lacryma-jobi 1-5 parts, Zea mays silk 1-5 parts, and Taraxacum mongolicum 1-5 parts. The traditional Chinese medicine composition of this invention has high safety, no toxic side effects, and exhibits synergistic effects among its components, resulting in good therapeutic efficacy and reduced adverse reactions.

The invention relates to the technical field of molecular biology, in particular to a flanking sequence of an insect-resistant transgenic maize AM11 exogenous insertion element and application of the flanking sequence. The flanking sequence is a 5 '-terminal flanking sequence of an exogenous insertion element, and is specifically a sequence as shown in SEQ ID NO.9 or a specific fragment of the sequence as shown in SEQ ID NO.9; the invention discloses an insect-resistant transgenic maize seed sequence, which is a flanking sequence at the end 3'of an exogenous insert fragment, particularly a sequence shown in SEQ ID NO.10 or a specific fragment of the flanking sequence, the flanking sequence is derived from an insect-resistant transgenic maize event AM11, the maize seed of the insect-resistant transgenic maize AM11 is preserved in China General Microbiological Culture Collection Center on September 5, 2025, the preservation number is CGMCC NO.46691, and the preservation number is CGMCC NO.46691. The corn is classified and named as corn Zea mays. The flanking sequence provided by the invention can be used for effectively judging whether the insect-resistant transgenic maize event AM11 is successfully obtained or not, so that a powerful guarantee is provided for monitoring the insect-resistant transgenic maize AM11 and progeny thereof and the like.

This invention belongs to the field of traditional Chinese medicine and relates to a traditional Chinese medicine composition for treating early diabetic nephropathy, its preparation method, and its application. The composition consists of: 15-45g of raw Astragalus membranaceus, 10-15g of Pseudostellaria heterophylla, 9-15g of Salvia miltiorrhiza, 9-15g of Paeonia lactiflora, 10-15g of Achyranthes bidentata, 3-6g of Pheretima aspergillum, 10-15g of Centella asiatica, 9-12g of Hedyotis diffusa, 15-30g of Euryale ferox, 9-12g of Broussonetia papyrifera, 9-12g of Mahonia japonica, 9-12g of Euonymus alatus, 10-15g of Poria cocos, 9-12g of Citrus reticulata peel, and 15-30g of Zea mays silk (wrapped and decocted). The beneficial effects of this invention are: strengthening the spleen and kidneys, replenishing qi and nourishing yin, promoting blood circulation and unblocking collaterals, and resolving blood stasis and eliminating turbidity. This medicine is used for early diabetic nephropathy caused by deficiency of both qi and yin, insufficiency of spleen and kidney, and internal obstruction of blood stasis and turbid toxins. The following symptoms may be observed: cloudy or foamy urine, soreness and weakness of the lower back and knees, fatigue and weakness, dry mouth and throat, mild edema of the limbs (especially the lower limbs), pale complexion, or numbness of the limbs, pale or dark red tongue, or with petechiae and ecchymosis, thin white or slightly greasy tongue coating, and thready or deep and thready pulse.