Corn plant having herbicide resistance trait through base-editing of epsps gene, and method for producing same

By employing base correction technology to mutate the EPSPS gene in corn, the method addresses the limitations of existing GMO-based herbicide tolerance development, enabling the creation of non-GMO, herbicide-tolerant corn plants that reduce import dependence and enhance accessibility for diverse companies.

WO2025110693A1 Publication Date: 2025-05-30TOOLGEN INC +1
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Patent Information

Application Number
PCT/KR2024/018347
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Priority Date
2023-11-21
Filing Date
2024-11-20
Publication Date
2025-05-30

AI Technical Summary

Technical Problem

Current methods for developing herbicide-tolerant crops, particularly corn, are dominated by multinational companies using GMO technology, which is expensive and time-consuming, limiting access for small and medium-sized companies. Additionally, there is a need for non-GMO alternatives to reduce public concerns and import dependence on GM soybeans and corn.

Method used

The development of a corn plant with a herbicide tolerance trait achieved through base correction of the EPSPS gene using cytosine base editors, which introduces targeted mutations in the EPSPS gene to confer resistance to glyphosate without causing double-strand breaks in DNA.

Benefits of technology

This approach allows for the creation of herbicide-tolerant corn plants that are scientifically proven to be non-GMO, potentially reducing the reliance on imported GM crops and making the development process more accessible and cost-effective for a broader range of companies.

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Abstract

The present invention relates to a recombinant vector comprising: a guide RNA expression cassette targeting the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene of corn (Zea mays L.); and a cytosine base editor expression cassette having a cytidine deaminase and a nuclease variant fused therein, and a method for producing a corn plant having a herbicide resistance trait through base-editing of the EPSPS gene using the recombinant vector.
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