249 results about "Vacuole" patented technology

The present invention relates to a transgenic plant, comprising one or more plant cells transformed with exogenous nucleic acid which increases expression of vacuolar pyrophosphatase in the plant. Also encompassed by the present invention are transgenic progeny and seeds of the transgenic plants described herein. Progeny transgenic plants grown from seed are also described. Plant cells (e.g., root cells, stem cells, leaf cells) comprising exogenous nucleic acid which increases expression of vacuolar pyrophosphatase in the plant cells are also the subject of the present invention. Also encompassed by the present invention are methods of making a transgenic plant described herein. The present invention also relates to a method of increasing the yield of a plant, a method of making a plant which is larger than its corresponding wild type plant, and a method of producing a transgenic plant with increased salt tolerance.

A heterogeneous mass comprising a longitudinal axis, a lateral axis, a vertical axis, one or more enrobeable elements and one or more discrete open cell foam pieces is disclosed. The open cell foam pieces comprise pores and vacuoles.

Methods of treating tissue with fractional laser radiation are disclosed. The fractional laser treatment methods reversibly increase skin permeability while maintaining a substantially intact stratum corneum and producing alterations within the epidermis and dermis. The alterations in the epidermis and dermis can include necrosis and / or coagulation. The alterations in the epidermis can include the creation of a plurality of pores in the stratum corneum and / or the creation of vacuoles in the layers of the epidermis below the stratum corneum. The fractional laser treatment methods disclosed herein can be used to provide treatments to the skin, to increase permeation of active substances into or through tissue, to deliver active substances locally or systemically, and to control the delivery of active substances.

Subjects treated with nonablative fractional photothermolysis (FP) have an intact stratum corneum, but can have microscopic lesions and vacuole formation within the epidermis. The vacuoles thus formed can trap dermal material and extrude it through the epidermis. Thus, FP can be used for the treatment of recalcitrant melasma, solar elastosis, and tattoos.

The present invention relates to a method which utilizes fresh aromatic plants as materials and combines the microwave heating technology with the vacuum technology to prepare essential oil. The method includes the following steps: the tissue structure of aromatic plant material is broken by cutting and grinding; after microwave irradiation is utilized to heat the aromatic plant material to a certain temperature, pressure is rapidly reduced to intensely gasify the moisture in the material so that the histiocytes, oil vacuoles and gland cells of the aromatic plants are broken; an aromatic substance is released from the cells and quickly volatilize; and the effect of the intense gasification and spreading of the moisture in the fresh material can effectively promote and intensify the volatilization of the aromatic ingredient, so that the abstraction of the essential aromatic plant oil can be fulfilled in a short time. The processing temperature in the process of the abstraction of the essential plant oil is low, the oxidation of sensitive ingredients can be avoided, the stability of the ingredients of the essential aromatic plant oil can be kept to the max, the time of abstraction is short, the efficiency is high, energy can be saved, and industrialized production can be conveniently realized.

The methods and apparatus disclosed herein can be used to treat glaucoma of the eye. The methods and apparatus can be configured to apply energy to the sclera, cornea, and / or other regions of the eye in order to shrink collagenous tissue near Schlemm's canal. Juxtacanalicular treatment of the sclera and / or cornea adjacent Schlemm's canal can be used to dilate Schlemm's canal, collector channels, and / or the Trabecular Meshwork. The methods and apparatus can be configured to apply energy to the sclera to generate scleral vacuoles in the sclera to improve uveoscleral outflow.

Compositions and methods for producing chitin and chitosan are provided. The compositions comprise genetically modified organisms, including fungi, yeast, bacterial and plant organisms that have been engineered to express heterologous genes involved in chitin and chitosan synthesis. Microorganisms and plants that have been modified for production of chitin and / or chitosan within the vacuole of a cell are encompassed. Methods for production of chitin also comprise culturing the genetically engineered organisms in conditions that allow for chitin production. Further methods include converting the chitin to chitosan by a chemical process. Production of chitosan also comprises culturing organisms that are genetically modified to produce chitosan without the need for chemical modification. Methods for in vitro chitosan production are also encompassed.

The invention relates to a pulse cavitation multiple jet nozzle, which comprises a nozzle body which is formed into an open end on one end and a jet end with a hollow cylinder on the other end, the open end is used for connecting with a fluid pipe, and the jet end is provided with a plurality of jet orifices; the central position of the jet end is provided with a central jet orifice, and a plurality of lateral jet orifices are arranged around the central jet orifice; the nozzle body is internally provided with an impeller being capable of rotating, a part of the fluid flowing into the nozzle passes through the center of the impeller and the central jet orifice to form continuous straight jet, the other part of the fluid impacts the blades of the impeller to cause the impeller to rotate so as to make flow field on the inlets of the lateral jet orifices around the central jet orifice generate regular disturbance, thus a pulse jet can be formed, vacuole can be produced, a cavitation jet can be formed, and the cavitation jet and the pulse jet are coupled to form a pulse cavitation jet to be ejected from the lateral jet orifices. The invention is simple in structure, and can greatly improve the efficiency of drilling.

The present invention relates to therapeutic and / or prophylactic uses of hydrazide compounds and to pharmaceutical compositions containing one or more of these compounds as an active component for treating a disease or disorder requiring modulation of vacuolar (H+)-ATPases.

The invention discloses a water-scavenger multifunctional environmental-friendly ship equipment and a method thereof, relating to a water-scavenger multifunctional environmental-friendly ship. The invention breaks fake vacuoles of blue-green algae cells by pressure so that blue-green algae loses necessary buoyancy so as to fast remove water-level blue-green algae and absorb the blue-green algae in kieselguhr organisms to be subsided to a lake bottom and absorbs nitrogen, phosphorus and organics contained in a lake to be automatically subsided to the lake bottom by a kieselguhr high-efficiency flocculant, so the purposes of fast lake water cleaning, denitrification and dephosphorization are achieved. The water-scavenger multifunctional environmental-friendly ship equipment has simple and compact structure, stable operation and high efficiency of blue-green removing at prows and denitrification and dephosphorization at sterns; the blue-green algae on a processed water body surface is obviously reduced; a water quality detection result shows that the invention can be used as an emergency measure when the blue-green algae is spread in large area; and in addition, a town lake water body repairing technology applying kieselguhr is a cheapest and most effective and efficient technology which has the best effect, fastest repair and effectiveness.

Methods and systems for method for acoustic treatment of tissue to disperse vacuoles within the tissue. Some of the present methods and systems comprise: directing pulsed acoustic waves from the acoustic wave generator into the tissue containing the vacuoles. Some of the present methods include indentifying the location of tissue containing vacuoles, and / or coupling (e.g., acoustically) an acoustic wave generator to the tissue containing the vacuoles.

Hydrophilic, arylsiloxy-containing monomers and macromonomers have at least a terminal hydrophilic group attached to an arylsiloxy group. The aryl groups attached to siloxy groups can be substituted with other hydrophilic groups. Polymers comprising such hydrophilic, arylsiloxy-containing monomers and macromonomers avoid or reduce the risk of forming vacuoles of absorbed water. Furthermore, such polymers have high refractive index, and, thus, are advantageously used for making ophthalmic devices, such as intraocular lenses, contact lenses, corneal rings, corneal inlays, and keratoprostheses.

The invention discloses a latex-enhanced immunoturbidimetry kit for measuring a Helicobacter pylori antibody as well as a preparation method and application thereof. The kit comprises a diluent, latex reagent and blank liquid of Helicobacter pylori antigen, a standard product and a quality control product, wherein the Helicobacter pylori antigen can be one or more of full-tropina antigen of Helicobacter pylori, urease antigen of Helicobacter pylori, cytotoxin-related protein A antigen of Helicobacter pylori or cell vacuole toxin A antigen of Helicobacter pylori; the latex reagent is a mixture of two latex granules which have different particle sizes and are adsorbed by Helicobacter pylori; and the Helicobacter pylori antibody for preparing the standard product and the quality control product is derived from IgM (immunoglobulin M) and / or IgG (immunoglobulin G) in serum of a patient infected by Helicobacter pylori. The kit is suitable for various full-automatic biochemical analyzers and semiautomatic biochemical analyzers, and has the advantages of rapid detection, high sensitivity, strong specifically, good accuracy and the like.

Methods for providing transgenic plants and parts hereof that, relative to the wild type state, is modified in a complex cell wall polysaccharide structure including pectins and hemicelluloses, the modification being in the overall glycosidic linkage pattern or the monosaccharide profile, comprising transforming a plant cell with a nucleotide sequence that causes an altered production of a complex cell wall polysaccharide-modifying enzyme such as endo-rhamnogalacturonan hydrolase, an endo-rhamnogalacturonan lyase, an endo-galactanase, an endo-arabinanase, an arabinofuranosidase, a galactosidase such as a beta-galactosidase, a xylosidase and an exo-galacturosidase. The modification can occur in vivo or post harvest, in which latter case the modifying enzyme is separated in the growing plant from its substrate, e.g. by targeting the enzyme to the Golgi, the endoplasmic reticulum or a vacuole, or is in a form that is inactive in the plant. After harvest the enzyme is brought into contact with its substrate or it is activated to provide the desired post harvest modification of the cell wall polysaccharide. The transgenic plant materials have improved functionalities and are useful in food and feed manufacturing and as pharmaceutically or medically active substances.

The present invention is related to methods and for producing higher titers of recombinant protein in a modified yeast host cell, for example Pichia pastoris, wherein the modified yeast cell lacks vacuolar sorting activity or has decreased vacuolar sorting activity relative to an unmodified yeast host cell of the same species. In particular embodiments vacuolar sorting activity is reduced or eliminated by deletion or disruption of a gene encoding Vps10 or a Vps10 homolog. The invention is also related to the modified yeast cells which are modified in accordance with the methods disclosed herein.

The invention is isolated nucleic acid molecules encoding Na+ / H+ exchanger polypeptides with 95% identity to a Na+ / H+ exchanger polypeptide from Arabidopsis thaliana for extrusion of monovalent cations from the cytosol of cells to provide the cell with increased salt tolerance. Crop species transformed with the nucleic acid molecule are capable of surviving in soil with high salt levels that would normally inhibit growth of the crop species.

The instant disclosure describes the application of genetic engineering techniques to produce cellulase in plants. Cellulase coding sequences operably linked to promoters active in plants may be transformed into the nuclear genome and / or the plastid genome of a plant. As cellulases may be toxic to plants, chemically-inducible or wound-inducible promoters may be employed. Additionally, the expressed cellulases may be targeted to vacuoles or other cellular organelles.

The invention discloses a tumor-targeted delivery carrier based on cell-derived micro-vacuoles, a preparation method and an application. The preparation method comprises the following steps of: (A) preparing a conditioned medium: supplementing fetal bovine serum, antibiotics, DSPE-PEG-Biotin and DSPE-PEG-Folate into a basal medium; (B) using the obtained conditioned medium in cell culture, and collecting cell culture supernatant for subsequent separation; (C) carrying out low-speed centrifugation on the obtained culture supernatant to remove cell debris and apoptotic bodies, then adding SA-IONPs, mixing uniformly, incubating, then separating by a magnet, using PBS for re-suspension, eluting for multiple times to obtain the cell-derived micro-vacuoles with membrane surfaces modified by folic acid and iron oxide nano-particles, and freezing for storage; and (D) loading chemotherapeutic drugs or therapeutic genes into the functionalized micro-vacuoles doubly-modified by an electroporation mode, and carrying out re-suspension after separation with the magnet. The tumor-targeted delivery carrier based on cell-derived micro-vacuoles, the preparation method and the application disclosed by the invention are applicable to specific targeting delivery of multiple chemotherapeutic drugs and therapeutic genes, and have the advantages of enhancing the anti-tumor effect, reducing the systemic toxicity and improving the clinical effect of the current therapeutic selection, so that a new hope is brought for clinical therapy of tumors.

The instant disclosure describes the application of genetic engineering techniques to produce cellulase in plants. Cellulase coding sequences operably linked to promoters active in plants may be transformed into the nuclear genome and / or the plastid genome of a plant. As cellulases may be toxic to plants, chemically-inducible or wound-inducible promoters may be employed. Additionally, the expressed cellulases may be targeted to vacuoles or other cellular organelles.

Subjects treated with nonablative fractional photothermolysis (FP) have an intact stratum corneum, but can have microscopic lesions and vacuole formation within the epidermis. The vacuoles thus formed can trap dermal material and extrude it through the epidermis. Thus, FP can be used for the treatment of recalcitrant melasma, solar elastosis, and tattoos.

The invention provides a method for preparing ultrafine polysulfonainide fiber. The method comprises the following steps: dissolving the polyphenylene sulfone terephthaloyl amide master batch prepared by a conventional method in a strong polar solvent to form 11 to 25 percent spinning solution; stirring the spinning solution for 30 to 90 minutes at a high speed and filtering the spinning solution; heating the spinning solution to a temperature of between 40 and 150 DEG C till regular vacuole occurs in the spinning solution; performing rapid pressurization on the spinning solution in a solution storage pool through an air duct; acting on the heated spinning solution by utilizing a metal electrode; after electric field force exceeds the surface tension of the spinning solution, forming mass injection streams on the top end of the vacuole; and subsequently, rapidly stretching and differentiating the injection streams to obtain the ultrafine polysulfonainide fiber. When the method is used to prepare polysulfonainide yarn, the production speed can reach between 50 and 200m / min, and the diameter of the polysulfonainide fiber is between 0.6 and 2.2 mu m and is only 1 / 8 to 1 / 10 of that of traditional polysulfonainide.

The invention relates to a self-adaptive biomimetic composite propeller blade. The body of the self-adaptive biomimetic composite propeller blade is formed by a guiding edge part and a following edge part. The self-adaptive biomimetic composite propeller blade is characterized in that the guiding edge part is arranged by taking the reference axis of a propeller as a center and provided with a rigid blade core, the rigid blade core is covered by a flexible material layer, and the flexible material layer extends along the chord length direction to form the following edge part. The self-adaptive biomimetic composite propeller blade has a simple and compact structure and is convenient to install. Due to the adoption of the self-adaptive biomimetic composite propeller blade, the overall manufacturing cost of the ship is reduced. Due to the adoption of the rigid blade core of the guiding edge part and the flexible material structure of the following edge part as well as the structure and the appearance of the propeller blade with the self-adaptive wake flow attack angle change, the violent bearing force vibration of the propeller blade working in the non-uniform wake field and the vacuole induced ship hull surface fluctuating pressure are reduced, the power of a host can be fully utilized in multiple working conditions, and the waste of the power of the conventional propeller host and the ducted propeller host can be reduced.

A shaped body, particularly a sorbent shaped body, made of microporous and mesoporous adsorbents and of composite adsorbents, which serve to store useful heat and useful cold, comprises a reduced proportion of binding agents. The sorbent shaped bodies have non-uniformly distributed cavities in the form of vacuoles that form gap volumes, and are obtained by placing the solid pre-mixed constituents of the shaped body onto a porous or perforated support. After adding the binding agent, the process of pre-solidifying the binding agent ensues by the action of an external force field, and this force field results in the formation of the non-uniformly distributed cavities. The proportion of binding agent on the support, which depicts the wall of the shaped body, is higher than that inside the shaped body. An optimally possible combining of the reaction efficacy of modified alumosilicates and / or aluminum silicates with their fluidic action during the exchange of materials and heat with the aim of achieving high space-time yields while storing useful heat and useful cold is thereby achieved.

The invention discloses a cyclone-static micro bubble flotation column ultrasonic enhanced pipe flow section mineralization device. The cyclone-static micro bubble flotation column ultrasonic enhanced pipe flow section mineralization device comprises an upstream narrow pipe section, an ultrasonic enhanced region, a downstream wide pipe section, an ultrasonic generator, a plurality of ultrasonic oscillators, a resonance screw and a hydrophone. The ultrasonic oscillators are arranged inside the ultrasonic enhanced region, the ultrasonic generator is connected with the ultrasonic oscillators, and the resonance screw is arranged in the ultrasonic enhanced region and the downstream wide pipe section; and the hydrophone is arranged in the ultrasonic enhanced region and the downstream wide pipe section. According to the cyclone-static micro bubble flotation column ultrasonic enhanced pipe flow section mineralization device, the evenly-distributed ultrasonic oscillators are adopted, so that spreading of ultrasonic waves in the ultrasonic enhanced region is facilitated, and sound waves are spread to the upstream and the downstream at the same time with the same transmitting characteristics. The ultrasonic enhanced region is arranged so that ore pulp flowing through the region can bear the strong ultrasonic effect, vacuoles can be generated, disturbance to flow can be generated, continuous generation of the vacuoles can continuously generate pulsating flow, and accordingly the turbulence intensity is greatly improved. The resonance screw has the effects of flow disturbance and turbulence intensity improving.

The subject invention includes non-naturally occurring salt tolerant plants that accumulate sodium in their tissues substantially in the vacuole. The present invention also includes methods of making such non-naturally occurring plants. One preferred method is generating a transgenic plant that has an ectopically expressed NHX related gene product.

A packaged, sterilized intraocular lens prepared by a process comprising providing a hydrophobic acrylic, or low water acrylic, intraocular lens and positioning the acrylic lens in a lens enclosure with an aqueous solution to provide a lens package. The lens package is then heated to a temperature sufficient for sterilization, however, the heating of the lens package must begin before the acrylic lens reaches an equilibrated, hydrated state following contact of the lens with the aqueous solution. The resulting sterilized acrylic intraocular lens will have less than sixty percent of total volume of disc-like features, or less than forty percent of total volume of water vacuoles, after 60 days following sterilization compared to an acrylic lens of the same composition, which was sterilized under the same conditions, but in an equilibrated, hydrated state.

The invention relates to a gene for improving cadmium tolerance of plants and application of an encoded protein of the gene in restoration of cadmium contaminated soil. The gene for restoring cadmium contaminated soil and improving cadmium tolerance of plants has a sequence shown as SEQ ID No:1 in a sequence table. The gene for improving cadmium tolerance of plants in the invention is transferred into the plants, so that the gene is excessively expressed in the plants, and the plant is cadmium tolerant. When the gene for improving cadmium tolerance of plants is excessively expressed, due to cadmium treatment, the gene can be combined with mannose, and increased expression of related genes such as GSH1, GSH2, PC1, PC2 and the like is promoted, so that the content of GSH and PC is increased. Outside cadmium ions are stored in vacuoles, tolerance to cadmium accumulation is further realized, and the cadmium contaminated soil can be restored to a certain degree.

The present invention provides an antiobesity composition comprising extract of Melissa as an active ingredient, use of Melissa extract, and a method for suppressing obesity using the composition. Also, the present invention provides an antiobesity composition comprising extract of Melissa and extract of Mori Folium as active ingredients, use of a mixture of Melissa extract and Mori Folium extract, and a method for suppressing obesity using the composition. Further, the present invention provides an antiobesity composition comprising extract of Melissa, extract of Artemisia and extract of Mori Folium as active ingredients, use of a mixture of Melissa extract, Artemisia extract and Mori Folium extract, and a method for suppressing obesity using the composition. When the present compositions are administrated into genetically obese mice or high fat diet-induced obese mice, the present compositions reduced body weight, body fat and gonadal fat, and reduce blood cholesterol level and obesity-related blood glucose level. Also, the present compositions suppress hypertrophy of adipocytes, and inhibit the accumulation of lipid in the liver by reducing the size and number of lipid vacuoles. Furthermore, in human clinial trial the present compositions show that body weight, PIBW, body fat, especially visceral fat, apolipoprotin B concentration, ratio of total cholesterol / HDL cholesterol and ratio of LDL cholesterol / HDL cholesterol are significantly decreased, and atherogenic index is improved and muscle mass is increased. Thus, the present compositions can be usefully applied as antiobesity composition for reduction of body weight and suppression of abdominal fat, particularly, visceral fat.

A micro vacuole generating device of a flat slab wall surface of array electrode type is used to reduce the frication resistance for the wall surface, belonging to the technical field of the fluid dynamics. The device consists of a top layer, a middle layer and a bottom layer. The top layer is gilded copper layer and serves as the anode of the power supply. The middle layer is a polyfluortetraethylene layer, serving as an insulated layer. The bottom layer is a copper layer, serving as the cathode of the power supply. The insulated layer is provided with millipores which are distributed to be arrays and the diameter is between 50 microns and 500 microns. The wall of the millipores is coated with the copper and connected with the bottom layer. The millipore shape is formed on the wall surface and the electrodes are distributed in the millipores shape. A water-electrolytic way is used to produce the micro vacuoles and consequently the distributed adsorption vacuoles are formed on the wall surface. In this way higher drag reduction efficiency can be achieved.