19 results about "Root cell" patented technology

This invention discloses Chinese kale BoaFLZ35 The application of genes in promoting vegetative growth of plants. This invention utilizes a gene-based approach to construct a *Gynostemma pentaphyllum* plant. BoaFLZ35 Heterologous overexpression of the gene in Arabidopsis thaliana was used to observe and analyze its effects on vegetative growth traits such as root elongation and rosette leaf morphology in transgenic plants. The results showed that heterologous overexpression... BoaFLZ35 This gene can significantly increase root cell length, thereby promoting the elongation of the Arabidopsis taproot; simultaneously, it can also increase leaf cell volume, resulting in significantly larger rosette leaves in transgenic plants. Therefore, it is evident that... BoaFLZ35 This is an important gene in the FLZ family of plants that positively regulates vegetative growth. This invention provides new insights into the function of FLZ family genes in the genus Brassica and also provides important candidate genes for high-yield molecular breeding of Brassica vegetables such as Chinese kale.

The invention discloses a planting method for guiding the growth of a main root system of a forest tree and improving the survival rate. The planting method comprises the steps of digging a planting pit, digging a guiding pit, applying a bag-controlled slow-release fertilizer, placing a dry corn cob core, placing a hollow plastic pipe, applying a root-promoting base fertilizer, backfilling soil, planting the forest tree, arranging a water collecting ring and topdressing. According to the method, a water collecting ring, a main root guide pit and a hollow plastic pipe are cooperatively arranged, a bag-controlled slow release fertilizer and a dry corn cob core are placed in the guide pit, and then a root-promoting fertilizer is applied to promote root system cell division. Water collected by the water collecting ring is drained to the guiding pit through the hollow plastic pipe and is absorbed and stored by the dry corn cob core. The root system is guided to grow downwards to absorb nutrients and moisture at the bottom of the guiding pit by utilizing the water and fertilizer tendency of the root system, so that the growth of the main root system is promoted, the stability and stress resistance of the forest are improved, the toppling is prevented, the planting survival rate is finally improved, and additional fertilizer can be applied from a plastic pipe opening.

The invention discloses application of a keratin related protein gene GbKAP related to cotton fiber quality characters, and belongs to the field of biotechnology application. The growth vigor and plant height of root cells, main roots, hypocotyls and rosette leaves of an arabidopsis thaliana plant for heterologous expression of the cotton keratin related protein gene GbKAP are superior to those of a control group. The GbKAP plays an important role and has an application prospect in promoting cell elongation, improving the quality character of cotton fibers and cultivating new varieties of high-quality cotton fibers.

The present application relates to the technical field of biological medicine, in particular to a pyrone compound, a preparation method and application thereof. The novel pyrone compound has a structure of formula 1, is separated into secondary metabolites of fungi Curvularia sp. ZYX-Z-4, and is found to have herbicidal effect through biological activity test evaluation. The pyrone compound has the advantages of low toxicity, high selectivity, target specificity and ecological friendliness when applied to herbicides. The herbicidal effect of the compound is evaluated by using model plants Arabidopsis thaliana. The results show that the pyrone compound has good inhibitory effect on the growth of Arabidopsis thaliana roots, can cause damage to root cytoplasmic membrane, and can cause root cell death. The pyrone compound can significantly inhibit the root length and fresh weight of three weeds, and further provides relevant theories and experimental basis for the development of biological herbicides.

The application discloses application of a GmPG39 gene in regulating soybean growth and phosphorus efficiency. The application research finds that the GmPG39 gene is involved in soybean root cell wall remodeling, the protein is expressed in the plant cell wall, and is regulated by the root system under the phosphorus, and is up-regulated in the main root section in response to low-phosphorus stress. Overexpression of the GmPG39 can increase polygalacturonase activity, reduce the pectin content in the cell wall of the hair root, aggravate the decomposition of the cell wall under low-phosphorus stress, and promote the relaxation of the cell wall. Meanwhile, the GmPG39 gene positively regulates the soybean growth and the phosphorus efficiency, under normal phosphorus conditions, overexpression of the GmPG39 gene can significantly promote the growth of the in-vitro hair root of the soybean, increase the fresh weight and the total root length of the hair root, increase the total phosphorus content and the soluble phosphorus concentration, and regulate the phosphorus absorption efficiency of the soybean, and has an important role in promoting the growth of the soybean root system and improving the phosphorus absorption efficiency of the soybean root system.

The application provides a Caragana korshinskii beta-D-xylosidase gene and application thereof in promoting stubble regeneration. The full-length of the Caragana korshinskii beta-D-xylosidase gene CDS is 2322 bp, contains 6 exons, encodes a polypeptide with 773 amino acids, and contains two glycosyl hydrolase domains. The plant overexpressing the beta-D-xylosidase gene has more vigorous growth ability, and the root is longer than that of the wild type. After stubble, the beta-D-xylosidase degrades polysaccharides in the root cell wall, and transports upwards, so as to provide energy for the regeneration process. The Caragana korshinskii beta-D-xylosidase gene and the above functions are discovered for the first time, so as to provide a target gene for analyzing the stubble regeneration mechanism of Caragana korshinskii and breeding high-quality Caragana korshinskii.

This invention provides a static resource allocation method for hybrid critical systems based on NUMA topology awareness, belonging to the field of computer technology. The method includes the following steps: Step 1: Parsing the platform hardware topology; Step 2: Defining node roles and delineating root cell boundaries; Step 3: Real-time domain resource planning; Step 4: Non-real-time extended domain resource planning; Step 5: Root cell resource convergence and retention; Step 6: Generating and solidifying the configuration. This invention fundamentally eliminates the impact of cross-NUMA node access on real-time performance and determinism.

The invention relates to the technical field of biology, in particular to antibacterial composite nanoparticle microspheres and application thereof in cultivation of ginseng seedlings. According to the antibacterial composite nanoparticle microspheres, the lactic acid bacteria and the witch hazel extract are adopted as antibacterial active substances, the lactic acid bacteria and the witch hazel extract are compatible with each other and have a synergistic effect, and the prepared nanoparticles have the effects of protecting root cells, inhibiting bacteria and enhancing plant resistance and can be controllably released along with acidity change; the resistance and the adaptability of the plant to an acid environment are enhanced. A multi-system continuous culture system is combined with a nano-composite microsphere mode, so that the survival rate, the oxidation resistance and the nutrient content of the ginseng seedlings are improved, and the resistance of the ginseng seedlings to an acid environment is enhanced. The ginseng seedlings obtained by the technology are used as extraction raw materials, the production cost is low, the production process is environment-friendly and pollution-free, and the method is suitable for popularization and application.

The invention discloses a preparation method of an active bacterium enzyme for strong rooting and seedling strengthening of crops, and relates to the technical field of crops. Comprising the following steps: screening to obtain a compound strain with rooting promoting activity, wherein the compound strain comprises bacillus subtilis, bacillus mucilaginosus and trichoderma harzianum; respectively carrying out expanding culture on the compound strains to obtain seed solutions of the strains; according to the application, the bacillus subtilis secretes plant growth regulators such as indoleacetic acid and the like to directly stimulate division and elongation of root system cells, meanwhile, antibacterial substances are generated to inhibit soil-borne diseases, the bacillus mucilaginosus can efficiently dissolve phosphorus and potassium, insoluble nutrients in soil are converted into available nutrients which can be absorbed by crops, nutritional support is provided for growth of seedlings, and the growth of the seedlings is promoted. Trichoderma harzianum can antagonize pathogenic bacteria, can generate chitinase and other enzymes, promotes thickening of root cell walls, and enhances the stress resistance of seedlings.

The invention relates to the technical field of nutrition injections, in particular to a nutrition injection for fruit tree trunks and a preparation method of the nutrition injection. The nutrition injection is prepared from the following raw materials: urea, a compound nutrient, an additive solution, cane sugar and water. In the preparation process of the nutrition injection for the fruit tree trunks, the ginkgo leaves, the rosemary and the lotus leaves are combined and added, and bilobalide components of the ginkgo leaves can promote division and elongation of root cells of fruit trees, so that the root systems of the fruit trees are more developed, and the water and nutrient absorption capacity of the fruit trees after transplanting is enhanced; rosmarinic acid has the effect of adjusting plant hormone balance and can stimulate fruit trees to root again at damaged positions of root systems after transplanting and improve the activity and absorption efficiency of the root systems, nuciferine is beneficial to improving the growth environment of the fruit tree root systems and promoting microbial activity of the fruit tree root systems, good conditions are provided for growth of the fruit tree root systems, and the yield of the fruit trees is increased. The yield and the transplanting survival rate of the fruit trees are effectively improved.

The invention provides an application of a dahurian angelica root expansion protein gene AdEXPA7 in regulation and control of root development. According to the application disclosed by the invention, the fact that the AdEXPA7 has a positive regulation effect on the elongation of root cells is determined by utilizing a biotechnology. Expression of an auxin response gene AdARFs can be induced by applying exogenous auxin to treat an angelica dahurica strain, and the auxin response gene AdARF8-2 is combined with an AuxRE cis-element on a promoter of an expansion protein gene AdEXPA7 of the angelica dahurica, so that expression of the AdEXPA7 is positively regulated and controlled. The root system, hypocotyl and root cell length of the arabidopsis thaliana obtained by over-expression of AdEXPA7 are significantly increased. Therefore, through overexpression of AdEXPA7, the invention has important breeding utilization value for promoting growth and development of arabidopsis thaliana roots.

This invention discloses a promoter specifically induced by low phosphorus in root cells and its application, relating to the field of genetic engineering technology. The key technical points are: the promoter has the nucleotide sequence shown in SEQ ID NO: 1. This invention also provides a vector containing the above promoter and its application in expressing the reporter gene GUS or the acid phosphatase gene OsPAP16 in rice. The beneficial effects of this invention are: the Pro-PRE provided by this invention is induced by low phosphorus and specifically expressed in root cells, driving the expression of exogenous genes, such as acid phosphatase or organic acid secretion proteins, under low phosphorus conditions, thereby improving the crop's tolerance to low phosphorus and providing important guidance for breeding low phosphorus-tolerant varieties.

The present application discloses a method for in situ culture of plant roots and its application. The method comprises: step 1, spreading sterilized Arabidopsis seeds flat on the upper edge of a glass slide culture medium; the thickness of the culture medium is 0.9-1.4 mm; step 2, placing the glass slide with seeds in a culture dish equipped with a culture device and culturing vertically under light; the culture device comprises a circular bottom plate, and a fixing device provided on the circular bottom plate for fixing the glass slide with seeds and a humidifying device for increasing the air humidity in the culture dish. After the vertical culture is completed, the present application can directly prepare slides without moving the Arabidopsis roots and observe the arrangement of microtubules in the root cells under a microscope, thereby avoiding damage and displacement of the Arabidopsis roots when preparing slides using traditional methods; specifically, by selecting a specific device during the vertical culture process, the technical problem that the existing culture dish cannot fix the glass slide, causing the root growth to bend and thus affecting the root observation is overcome.

The invention discloses a ginseng adventitious root cell strain CCUM-Re-7 as well as an extract, a preparation method and application thereof. A construction method of the cell strain CCUM-Re-7 comprises the following steps: performing double disinfection and sterilization on ginseng, performing culture amplification after slicing, and separating into layered stem cells; inducing the separated cambium stem cells to generate ginseng adventitious roots; culturing the induced ginseng adventitious roots, and screening a cell strain CCUM-Re-7 from the induced adventitious roots; and carrying out large-scale culture on the cell strain to extract and prepare the ginseng adventitious root extract rich in saponin, and taking the ginseng adventitious root extract as an effective component to prepare the anti-aging and whitening composition. The invention belongs to the technical field of biology, and particularly provides a method for extracting an extract rich in ginsenoside Re from ginseng adventitious roots induced by ginseng cambium stem cells and application of the extract in anti-aging and skin whitening.

The invention relates to the technical field of biology, in particular to a naphthoquinone derivative and a preparation method and application thereof. A series of novel naphthoquinone compounds are found from Fusarium sp.L-3 secondary metabolites for the first time, and the influence of treatment of the naphthoquinone derivatives on the growth of purslane roots, the influence of treatment of the naphthoquinone derivatives on the integrity of purslane root cell membranes, the weeding spectrum, the action mechanism and the toxicity of the naphthoquinone derivatives are studied. Results show that the naphthoquinone derivative can significantly inhibit growth of portulaca oleracea roots, destroy plasma membrane integrity and cause root cell death, plays a role mainly through a photosynthetic transmission chain plasma quinone competitive site, is safe and non-toxic, has the advantages of wide herbicide controlling spectrum, significant effect and unobvious toxicity, and meanwhile, has a simple structure, is easy for industrial production and has a broad application prospect. The bacillus subtilis has the potential of being developed into biological herbicides.

The application discloses a LLC allocation method and system based on Jailhouse and a medium, and the method comprises the following steps: formulating a LLC allocation strategy, determining the mapping relationship between each way group of LLC and each scheme, binding a scheme id for each CPU core, filling in a configuration file, writing the LLC allocation strategy as allocation configuration information, and writing the allocation configuration information into a root cell configuration file of Jailhouse, LLC allocation strategy issuing, in the enabling stage of Jailhouse, parsing the root cell configuration file, extracting the allocation configuration information therein, and configuring relevant hardware registers according to the allocation configuration information. The application can overcome the LLC cache conflict between cells, has high memory utilization and good scalability, and can enable Jailhouse to perceive and manage LLC configuration.

The application discloses application and methods of polyaspartic acid in improving the cadmium fixation capacity of plant root cell walls and reducing cadmium accumulation in the aboveground part, and belongs to the technical field of agricultural environment. The polyaspartic acid can promote the distribution of cadmium in the root cell wall, hinder the transportation of cadmium to the aboveground part through the xylem, thereby reducing the cadmium content of the aboveground part of the plant, relieving the cadmium toxic stress of the aboveground part, and reducing the influence of the edible part of the aboveground crop on human health. In addition, under cadmium stress, the application of the polyaspartic acid can reduce the damage of cadmium to the structure of plant chloroplasts, increase the content of photosynthetic pigments, and promote the accumulation of plant biomass. Therefore, the application of the polyaspartic acid in improving the cadmium fixation capacity of plant root cell walls and reducing cadmium accumulation in the aboveground part has a positive significance for guaranteeing food safety.

The invention discloses a biomass charcoal synergistic probiotic improved matrix and a preparation method and application thereof, and belongs to the technical field of solid waste resource utilization, the porous structure of biomass charcoal is utilized to provide a stable attachment carrier for probiotics, the high cation exchange capacity and water retention characteristic of the biomass charcoal are utilized to improve the utilization rate of the probiotics, and the utilization rate of the biomass charcoal is improved. The bacillus subtilis and the metabolic activity of the probiotics are matched to form a synergistic effect, auxin secreted by the bacillus subtilis after rhizosphere colonization can directly stimulate division and elongation of plant cells, and the phosphorus dissolving capacity of the auxin improves the effectiveness of matrix nutrients; the filiform network of piriformospora indica can reinforce soil aggregates and reduce pore blockage, meanwhile, generated phosphatase converts insoluble phosphate into an absorbable form, active absorption of nutrients by root cells is promoted through signal exchange, and when the filiform network of piriformospora indica and the phosphatase are applied in a compound mode, the proportion of matrix ventilation pores to water holding pores is more adaptive, and the nutrient absorption rate is increased. Supply of quick-acting potassium and available phosphorus is more sufficient, and double guarantee is provided for root growth and nutrient transfer of hydrangea.

The invention discloses a near-infrared fluorescent probe for detecting H2S in a biological system as well as a preparation method and application of the near-infrared fluorescent probe. The structural formula of the fluorescent probe is shown in the specification, and the probe has excellent mitochondrial targeting ability and can be successfully applied to H2S detection and fluorescence imaging in a biological system. By virtue of the unique dynamic response characteristic, the probe can quickly distinguish H2S from other sulfur-containing biological small molecules and amino acids. And obvious color change is accompanied in the reaction process, so that naked eye observation is facilitated. The probe can be used for effectively monitoring changes of endogenous and exogenous H2S in onion endoepidermis cells, soybean slice cells, soybean root cells, tobacco root cells and wheat root cells under stress conditions, and is suitable for whole-plant fluorescence imaging analysis of mung bean plants and peanut plants. On the other hand, the prepared fluorescent probe is successfully applied to co-localization imaging of wheat root mitochondria, and real-time monitoring of H2S in juvenile zebrafish bodies is achieved.