Method for producing ginsenoside and polysaccharide by cell culture method

A ginsenoside and cell culture technology, applied in plant cells, steroids, organic chemistry, etc., can solve the problems of harsh conditions, complex process, low yield, etc., and achieve improved yield, simple process, and improved product yield. Effect

Inactive Publication Date: 2012-08-15
江西中天医药生物有限公司
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Problems solved by technology

The technology of producing ginsenosides, monomers and polysaccharides by using cell biology technology has been studied at home and abroad for a long time, and many achievements have

Method used

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Embodiment Construction

[0021] In order to make the technical means, creative features, goals and effects achieved by the present invention easy to understand, the present invention will be further elaborated below in conjunction with examples.

[0022] 1. Cell culture stage:

[0023] (1) Seed cell collection:

[0024] ①Clean and disinfect fresh perennial ginseng roots, soak the washed ginseng tubers in 10% sodium hypochlorite solution for 30 minutes, and wash the tubers with sterile water until no sodium hypochlorite remains;

[0025] ②Put the cleaned roots into a beater and add MS medium, add 10ml per 1.5g, and beat;

[0026] ③ Add cellulase 100mg / kg to the beating solution, let it stand for 3 hours, filter it with a microporous filter with a pore size of 50 microns, separate the filtrate with a low-speed centrifuge, remove impurities and collect the seed cells from the supernatant;

[0027] (2) Seed cell culture:

[0028] ①Inoculate the collected seed cells into the seed tank (bottle) containin...

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Abstract

The invention discloses a method for producing ginsenoside and polysaccharide by a cell culture method. According to the method, the root cell culture of perennial ginsengs is utilized and an enzymatic technology is adopted to shorten the cell culture cycle to produce the ginsenoside and the polysaccharide. The method adopts the cell culture method to culture and produce ginseng plant cells and then the ginsenoside and the polysaccharide are extracted. Cellulose is added when seed cells are collected to increase the collection rate of the seed cells by 5-10%. During the culture of the seed cells, growth hormone and pectinase are added to induce the histocytes to accelerate growth and reproduction and the culture cycle can be shortened by 1/4 to 1/6, so that the infection rate during the culture process is effectively reduced and the production yield is increased. The method is simple and stable in process and the enzymatic technology is utilized to shorten the cell culture cycle and improve the yield, thus the method is applicable to industrial production.

Description

technical field [0001] The invention relates to the production technology of glycosides and sugars, in particular to the production technology of ginsenosides and polysaccharides, specifically a method for producing ginsenosides and polysaccharides by cell culture. Background technique [0002] Ginsenoside is the main component of ginseng, which can delay the aging of nerve cells and reduce the memory damage in old age. It can stabilize the membrane structure and promote protein synthesis, which can improve memory. Ginsenosides, monomers, and polysaccharides are widely used in many fields such as food, health products, medicine, and chemical industry. With the rapid development of society, the amount of ginsenosides and polysaccharides is getting higher and higher. However, the extraction of traditional ginsenosides and polysaccharides The method is to extract the ginseng roots, stems, leaves, fibrous roots and other plant bodies, and the products obtained by this process ca...

Claims

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Application Information

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IPC IPC(8): C12N5/04C07J17/00C08B37/00
Inventor 高国文李成林
Owner 江西中天医药生物有限公司
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