Method for producing ginsenoside and polysaccharide by cell culture method

Abstract

The invention discloses a method for producing ginsenoside and polysaccharide by a cell culture method. According to the method, the root cell culture of perennial ginsengs is utilized and an enzymatic technology is adopted to shorten the cell culture cycle to produce the ginsenoside and the polysaccharide. The method adopts the cell culture method to culture and produce ginseng plant cells and then the ginsenoside and the polysaccharide are extracted. Cellulose is added when seed cells are collected to increase the collection rate of the seed cells by 5-10%. During the culture of the seed cells, growth hormone and pectinase are added to induce the histocytes to accelerate growth and reproduction and the culture cycle can be shortened by 1 / 4 to 1 / 6, so that the infection rate during the culture process is effectively reduced and the production yield is increased. The method is simple and stable in process and the enzymatic technology is utilized to shorten the cell culture cycle and improve the yield, thus the method is applicable to industrial production.

Description

technical field [0001] The invention relates to the production technology of glycosides and sugars, in particular to the production technology of ginsenosides and polysaccharides, specifically a method for producing ginsenosides and polysaccharides by cell culture. Background technique [0002] Ginsenoside is the main component of ginseng, which can delay the aging of nerve cells and reduce the memory damage in old age. It can stabilize the membrane structure and promote protein synthesis, which can improve memory. Ginsenosides, monomers, and polysaccharides are widely used in many fields such as food, health products, medicine, and chemical industry. With the rapid development of society, the amount of ginsenosides and polysaccharides is getting higher and higher. However, the extraction of traditional ginsenosides and polysaccharides The method is to extract the ginseng roots, stems, leaves, fibrous roots and other plant bodies, and the products obtained by this process ca...

AI Technical Summary

Problems solved by technology

The technology of producing ginsenosides, monomers and polysaccharides by using cell biology technology has been studied at home and abroad for a long time, and many achievements have been made, but there are still many difficult problems in the process of industrial production and promotion, such as complex process, harsh conditions, easy Pollution, low yield, etc.