1062results about How to "Short training period" patented technology

The invention discloses a composite efficient microbial preparation for treating difficultly-degradable waste water. The composite efficient microbial preparation comprises the following active ingredients of: bacillus, pseudomonas, alcaligenes eutrophus, aspergillus and yeast. The composite efficient microbial preparation has a unique treating effect on high-concentration organic sewage and highammonia and nitrogen sewage which are difficult to treat by the conventional activated sludge process and contain macromolecules and difficultly-degradable harmful constituents.

The invention provides a cardiovascular interventional virtual surgery simulation system based on force feedback. The cardiovascular interventional virtual surgery simulation system comprises a displacement collecting unit, a force feedback mechanism, a motor driver, a processor and a computer, wherein the displacement collecting unit comprises a guide wire displacement collecting unit, a balloon catheter displacement collecting unit and a guiding catheter displacement collecting unit; and the force feedback mechanism comprises a guide wire force feedback mechanism, a balloon catheter force feedback mechanism and a guiding catheter force feedback mechanism. The cardiovascular interventional virtual surgery simulation system is used for simulating the movement sense and the force sense in the process of inserting guide wires, catheters and the like in vascular intervention therapy. The surgery accuracy is high, the repeatability is good, the training cycle of doctors can be shortened, and the surgical risk is reduced.

The invention discloses a processing method for dendrobium candidum health-card tea, which mainly comprises the following steps of: pre-processing, airing, drying, de-enzyming, rolling, initially drying, forming, drying and enhancing aroma. According to the processing method, reasonable development and utilization of tissue culture seedling during production of dendrobium candidum seedling is realized, the dendrobium candidum health-card tea has unique aroma which ordinary tea does not have, and can be eaten after drinking, a sticky feeling is generated by chewing, and moreover, the dendrobium candidum health-card tea is low in processing cost, and is simple to prepare, easy to preserve and much convenient to drink.

A combined equipment for artificially culturing plants is composed of a number of hexagonal transparent culture containers which are combined in cellular mode, the overflow unit of nutritive liquid, nano lamp, humidity controller, CO2 source, speed-adjustable motor, blower and pipelines. Its advantages are low pollution, high quality of seedlings and short culture period.

The invention discloses a method for primary culture of tumor cells and relates to the field of cell biology. According to the method disclosed by the invention, a tumor sample is prepared into a single-cell suspension liquid by use of an enzyme method or a mechanical method, and then tumor cell bladders are prepared by use of a serum-free suspension culture method, wherein alkaline fibroblast growth factors, epidermal growth factors, insulin and bovine serum albumin need to be added during a culturing process, then the growth factors are removed, and the culture is implemented in a general culture medium to obtain adherent cells capable of realizing continuous passage. The method disclosed by the invention is short in culture period and has the effect of increasing the success rate of the primary culture of tumors.

The invention provides freshwater chlorella sorokiniana XJ02 and application thereof in fixation of CO2 and production of microalgae oil. The preservation number of the chlorella is CCTCC (China Center For Type Culture Collection) NO: M2013104. The chlorella disclosed by the invention can be used for fixing 0.03-20% of CO2 at high efficiency, the CO2 fixation efficiency of chlorella is 130-343mg / L / d, the biomass (dry weight) concentration of the chlorella is 720-2250mg / L, the oil content of chlorella is 29.2-41%, the oil fatty acid of the chlorella is mainly composed of C16 and C18 short-chain fatty acids, and the chlorella is suitable for production of biodiesel. Compared with the reported microalgae, the chlorella has higher CO2 fixation efficiency and biomass yield; and the chlorella can be used for obtaining the microalgae oil at high yield by selecting an appropriate culture condition, thus the production cost of the microalgae oil can be greatly reduced. An excellent production algae is provided for high-efficient fixation of CO2 in the environment and preparation of biodiesel by the microalgae oil.

The invention discloses a drawing and loading device for cells, which relates to a cellular mechanics loading device that belongs to the medical instrument. The drawing and loading device for cells consists of a control part, a mechanical part and an electromagnetic part, which is characterized in that: the cells are cultivated on an elastic silicon membrane, and circumferential-direction drawing and compressive loading are carried out for the cells through a dynamic system, meanwhile, magnetic bead collagen with ferroferric oxide enveloped is attached to the surfaces of the cells through integrin family, then alternating magnetic field is respectively loaded at an upper and a lower bottoms of a cell cultivating box, rendering the cells to be drawn under the magnetic force while being under the circumferential stress in a base strain field, so as to control the transformation of the cells along the axis Z and control the cell morphology. By simulating the borne mechanical stress of cells under physiological conditions, the drawing and loading device for cells can realize mechanical loading with different dimensions under different mechanical forces, and has the advantages of simple structure, convenient use, wide application and excellent reproducibility, etc.

The invention provides a timed and circulating AM (arbuscular mycorrhizal) fungus expanding propagation and functional experiment device and relates to culture of soil microorganisms. The device is provided with an incubator cover, an incubator, a culture medium, a space for adding an isotope and the like, a hypha chamber, a water drainage tank cover, a water drainage tank, a filter pump, a plant growth chamber, a nozzle regulation and control hook and a nylon net, wherein the incubator cover covers the incubator, the incubator is divided into the hypha chamber and the plant growth chamber by virtue of a longitudinal net, the culture medium and the space for adding the isotope and the like are placed in the hypha chamber, and a water drainage hole is formed in the bottom of the incubator; the water drainage tank is arranged at the bottom of the incubator, the water drainage tank cover covers the water drainage tank, the filter pump is arranged in the water drainage tank, the nozzle regulation and control hook and the nylon net are arranged in the plant growth chamber, an atomizing nozzle is formed in the nozzle regulation and control hook, the atomizing nozzle and the filter pump are connected by virtue of a PVC hose, and a plant fixing device is arranged on the top of the plant growth chamber.

The invention relates to a porous bacterial cellulose skin repair material with a density structure and a preparation method thereof. The porous bacterial cellulose skin repair material with the 'upper dense and lower loose' structure similar to human skin is prepared by controlling the culture condition of bacterial cellulose and adding sustained-release microspheres in the culture process. A loose layer is tightly combined with a dense layer, so that obvious physical layering is avoided, and the structural continuity is good; and gradient changes of the structures are produced in the loose layer and the dense layer, and multiple pores distributed uniformly are formed in the loose layer, so that the upper dense and lower loose gradient structure of the human skin is simulated to the utmost degree. Cells easily enter the material, the healing period of a wound is obviously shortened, and proliferation of healed scars is effectively reduced; and good air permeability and water holding property of the bacterial cellulose are kept, the wound surface can be kept in a wet environment, and healing of the wound surface is facilitated. The forming process is simple, the culture period is short, the preparation process is environment-friendly, simple, convenient and quick, and the preparation cost is low.

The invention belongs to the field of microalgae culture and discloses a formula of a Dicrateria zhanjiangensis improved culture medium and a simple culture technique of Dicrateria zhanjiangensis. Dicrateria zhanjiangensis is traditionally cultured by using an f / 2 culture medium in an open cement pit manner, so the doubling time of algae cells is long, the grow speed is low, the investment is high, the yield is low, pollution is easily caused, and the operation is troublesome. When Dicrateria zhanjiangensis cells are cultured by using the culture medium provided by the invention and mineral water barrels, the content of unsaturated fatty acid is high, the cost is low, pollution is avoided, the operation is easy, and the workload is greatly reduced; and nutrients such as sodium hydrogen carbonate, ferric citrate, potassium sorbate, glucose, sea mud extract, soil extract, ethylenediamine tetraacetic acid complexing agent and cow dung and goat manure extract are added in the culture medium, and organic fertilizers and inorganic fertilizers are used together, so that more nutrients are available, the growth speed of the Dicrateria zhanjiangensis is greatly improved, the culture period is shortened, and the yield is improved by 92%.

The invention aims at providing an automatic analyzing method and system for vaginal microecological morphology. A low-resolution smear image obtained through conventional equipment is subjected to super-resolution amplification rebuilding to be clearer, a foundation is laid for follow-up classification, then, a nerual network technology is applied for judging and recognizing the rebuilt high-resolution image, and the variety and density of microorganisms are further output, so that the purpose of carrying out automatic analysis and computer assisted recognition on vaginal microecological morphology is achieved, and objective and accurate clinic evaluation is carried out on a vaginal microecological system.

The invention discloses a method for cultivating tissues to obtain seedlings of dendrobium huoshanense at one step. The method includes steps of preparing solid cultivation media; preparing liquid nutrient liquid according to a formula of the solid cultivation media; selecting and disinfecting dendrobium huoshanense capsules; preparing dendrobium huoshanense seed suspension liquid; sowing the dendrobium huoshanense seed suspension liquid in an aseptic condition; cultivating the seedlings; germinating the seedlings. The solid cultivation media are solid nutrient media, and the solid nutrient media in a formula comprise macroelements, trace elements, iron salt, organic components, hormone and additives. The liquid nutrient liquid is free of hormone, agar, chlormequat and banana and apple extracted liquid. The method for cultivating the dendrobium huoshanense has the advantages that the dendrobium huoshanense cultivation period can be shortened, the seedlings can be germinated in 6 months, in other words, the seedlings can be germinated in exactly seedling planting periods in April of a second year if seeds in October of a first year are sown, accordingly, the production cost for cultivating the seedlings of dendrobium huoshanense from the tissues can be reduced by 50%, the planting input can be reduced, and the cultivation survival rate and the cultivation germination rate can be increased.

The invention discloses a method for breeding black soldier fly larvae. The method includes steps of S1, uniformly mixing livestock and poultry feces which is fermented at the high temperatures, wheatbran, humus, brown sugar and clostridium butyricum with one another according to a mass ratio to prepare black soldier fly breeding bait; S2, breeding the larvae, to be more specific, inoculating 1-2g of newly hatched black soldier fly larvae in every 1 kg of black soldier fly breeding bait, carrying out culture on the newly hatched black soldier fly larvae at the temperatures of 25-35 DEG C for6-8 days and carrying out screening to obtain the mature black soldier fly larvae. The mass ratio of the livestock and poultry feces to the wheat bran to the humus to the brown sugar to the clostridium butyricum is 900-1000:40-60:20-40:4-5:1-3. The method has the advantages that growth and ingestion of the black soldier fly larvae can be promoted by the aid of the method, the livestock and poultry feces consumption speeds can be increased, the culture cycle of the black soldier fly larvae can be shortened, the protein content of black soldier fly can be increased, the black soldier fly larvaeobtained by the aid of the method can be used as a raw material for active feed or protein-source feed, and the method has a great application prospect.

A citric acid aspergillus niger seed continuous culture method based on a mycelium pellet dispersion technology comprises the steps as follows: (1), an aspergillus niger spore solution is inoculated to a seed culture medium and cultured and prepared into a mature seed solution; (2), the mature seed solution is treated by a disperser to obtain a dispersed mycelium seed solution; (3), part of the dispersed mycelium seed solution obtained in the step (2) is transferred to a fermentation medium for fermentation with 5%-15% of inoculum concentration, and the fermentation is finished when the reducing sugar concentration of the fermentation medium is lower than 0.5%; and (4), part of the dispersed mycelium seed solution obtained in the step (2) is inoculated to a seed culture medium with 5%-15% of inoculum concentration to be cultured and prepared into a next level of mature seed solution, and then the operation returns to the step (2), so that the continuous culture for aspergillus niger seeds is realized. According to the aspergillus niger seed continuous culture method provided by the invention, the tedious culture process of aspergillus niger spores can be directly reduced, simultaneously, the culture time of mature seeds can be effectively shortened, and the utilization rate of raw materials is increased.

The invention relates to a method for increasing biomass and astaxanthin content of haematococcus pluvialis, and belongs to the technical field of bioengineering. The method comprises steps as follows: a BBM culture medium with sodium acetate or magnesium acetate added is prepared firstly, after being sterilized, the BBM culture medium is inoculated with the haematococcus pluvialis cultured to theexponential phase until the concentration reaches 2.0*10<5>-2.5*10<5> cells / mL, culturing is performed at the temperature of 24-26 DEG C under the condition of light intensity being 2000-2500 lx, andsampling is performed every other day for biomass measurement; algae cells are collected when the biomass is maximum, diluted by the nitrogen-deficient BBM culture medium with melatonin added until the concentration is 2.0*10<5>-2.5*10<5> cells / mL, culturing is performed at the temperature of 26-28 DEG C under the condition of light intensity being 12000-14000 lx, and sampling is performed everyother day for astaxanthin content measurement. The method is simple and easy to operate, growth cycle of the haematococcus pluvialis can be shortened to a certain degree, and the biomass and the astaxanthin content of the haematococcus pluvialis are increased.

The invention discloses an artificial culture method of Zn-enriched cordyceps militaris mycelia and a culture medium thereof; according to weight percentage, the formulation components of the culture medium comprise: 1.0 to 3.0 percent of glucose, 0.5 to 1.0 percent of peptone, 0.1 to 1.0 percent of yeast extract, 1.0 to 2.0 percent of agar powder and the rest of water, and the pH value of the culture medium is 5.0 to 7.5. The artificial culture method comprises the steps of test tube slant surface strain culture (activation), test tube liquid strain culture, triangular flask liquid seed culture, seeding tank culture, fermenter culture, plate-frame filtration, crashing, drying in vacuum, crashing and leading the mycelia into powder. The operation is simple, the culture period is short, the yield of mycelia is high, no pollution and waste are produced, and the method has the function of supplementing zinc, thus being capable of carrying out industrial production.

The invention provides a method for acquiring a safe and effective umbilical cord mesenchymal stem cell. A pollution-free umbilical cord mesenchymal stem cell can be prepared by umbilical cord acquisition, umbilical cord transportation, umbilical cord preparation and methods of detecting, screening, adding antibiotics and the like in a cell culturing process, so that the safety can be guaranteed; and improved culture system and culture method are adopted in the separating and culturing processes, so that the separation efficiency is improved, the culturing cycle is shortened, and a high-purity excellent-characterization cell can be obtained. According to the method, the cost can be effectively reduced, the high-quality umbilical cord mesenchymal stem cell can be quickly acquired, injury on cells can be reduced, the culturing cycle can be shortened, pollution can be reduced, and the cell purity can be improved.

The invention discloses a high-yield mixing culturing method of phaeodactylum tricornutum bohlin and nitzschia closterium, aiming at solving the problems that the phaeodactylum tricornutum bohlin and the nitzschia closterium are individually cultured with an f / 2 culture medium in an open cement pit, which causes low growth rate, low yield, easy polluting, and complex operation in the traditional culture method. The culture medium, disclosed by the invention, is characterized in that a mineral water barrel is utilized for mixing culturing of the phaeodactylum tricornutum bohlin and the nitzschia closterium, thus high fatty acid content can be obtained, the cost is low, the pollution is difficult to cause, the operation is easy, and the workload is greatly decreased; in addition, peptone, agar, maltose, potassium sorbate, glucose, sea mud extracting liquor, soil extract liquor, human urine, and cow and sheep manure leaching liquor are added to the culture medium, so that the nutrition is comprehensive and balanced, the growth speed of algae can be greatly raised, and the output is increased by 180%; moreover, the mixing culturing enables the mutualism of the two kinds of algae, and the two kinds of algae can absorb advantages mutually so as to promote each other; nutritive salt is fully utilized, so that the economic benefit is also increased.

The invention relates to a clonal tissue culture breeding method for a camphor tree and belongs to the technical field of forest tree tissue culture. The clonal tissue culture breeding method comprises the steps of explant material sterilization, bud induction, bud propagation, rooting induction, rooted-seedling hardening, and transplantation and management of tissue-cultured seedling, and particularly comprises the following steps of: removing the leaves of an annual shoot; cutting the shoot into stems which are respectively provided with 1-2 axillary buds; sterilizing the stems; carrying out induced culture by an inducing medium; carrying out enrichment culture and rooting induction on the obtained new bud; hardening and naturalizing the obtained rooted seedling; and finally transplanting the naturalized seedling into a matrix which is sterilized, and managing the transplanted seedling. Seedling raising by the clonal tissue culture breeding method for the camphor tree, disclosed by the invention, is not affected by natural factors such as season and weather, and besides, production cost is low, land resources are saved, and seedling raising efficiency is improved; meanwhile, with adoption of the clonal tissue culture breeding method disclosed by the invention, the phenomenon of browning can be effectively prevented, the effective reproduction rate is high, the rooted seedling is grown regularly, the culture period is short, the survival rate for the transplanting of the tissue-cultured seedling is high, and the seedling stage culture period is short; and the clonal tissue culture breeding method is significant.

The present invention discloses a method for quick and artificial cultivation of Nostoc sphaeroids Kutz which comprise: preparing suitable cultivation liquid, screening original stock seed, disinfecting original stock seed, liquid inoculation and culture, algae germplasm formation and purification, amplified cultivation. In the present method of artificial cultivation of Nostoc sphaeroids Kutz , direct liquid inoculation and culture can be employed through optimum culture liquid without need of antibiotic sterilization and purification or solid culture medium purification process, which has short cultivation cycle and yield germplasm in large volume and operate easily compared to the existing technology, which fit the need of cultivating Nostoc sphaeroids Kutz in large scale.

The invention discloses a device for cultivating anaerobic granular sludge. The device is characterized in that an artificial sludge bed is arranged inside a body and is formed in a manner that a packing support pipe is fixed between an upper support plate and a lower support plate in a penetrating manner; the upper support plate and the lower support plate are fixed together with the body through a lower fixing frame; radial packings are evenly distributed on the pipe wall of the packing support pipe; a plurality of paths of inlet water distributors are arranged at the bottom part of the body and are connected with a water inlet pump; a water outlet pipe is arranged at the upper part of the body; an overflow weir is arranged on the body above the water outlet pipe; an exhaust pipe is arranged on the body above the overflow weir and is connected with an air collector; and a sludge pump connected to the lateral wall of the bottom part of the body is connected with a sludge collecting tank. The invention also provides a method for cultivating the anaerobic granular sludge. A strain cultivated by the device and the device is stable in performance and has a strong capacity of adapting to changes of an external environment, an expansion coefficient of the anaerobic granular sludge bed is high, a cultivation period of the anaerobic granular sludge is short, the cultivation efficiency is high, and the performance of the anaerobic granular sludge is stable.

The invention discloses a rapid proliferation method for anoectochilus formosanus tissue culture, which comprises the steps: cleaning and disinfecting an explant, inoculating the explant after cleaning and disinfecting in an MS culture medium, performing low light culture for one week, then performing strong light culture for one week, wherein the illumination time is 10 hours per day, and the temperature is 23+ / -2 DEG C; culturing for 40 days; inoculating the explants after culturing in a liquid culture medium of the MS culture medium, 1.4-1.6mg / L of 6-BA and 0.2-0.3mg / L of NAA in a ratio for enrichment culture under a dark culture condition, wherein the temperature is 23+ / -2 DEG C, and the culture period is 45 days; and by using a strong seedling culture medium as the MS culture medium, performing illumination culture, wherein the illumination time is 8 hours per day, the temperature is 23+ / -2 DEG C, the culture period is 12-15 days, and the ratio of a rooting culture medium is as follows: the content of MS is 1 / 2, the content of bananas is 60g / L, the content of active carbon is 1g / L, the content of casein hydrolysate is 0.5g / L, the illumination time is 10 hours per day, the temperature is 23+ / -2 DEG C, and the culture period is 3 months. According to the rapid proliferation method for the anoectochilus formosanus tissue culture, the industrialized rapid proliferation is realized, and the culture period is shortened.

The invention discloses a plant tissue culture rooting and seedling exercising integrated cultivating device and a cultivating method thereof. The cultivating device comprises a plant cultivation system, an illumination system, a ventilation temperature-control system, a fluid supplying system, a CO2 supplying system and an environment control system. The plant cultivation system comprises a three-dimensional multi-layer cultivation frame and cultivation containers placed on the cultivation frame, each layer of multiple layers of the cultivation frame is provided with one cultivation container, and lifting seedling disc supports are arranged at the bottoms of the containers and used for suspending cultivation plants. The illumination system comprises lamp boxes, LED lamp tubes and fans, and the LED lamp tubes are arranged inside the lamp boxes and connected with the fans to be installed above the cultivation containers in an integrated mode without communication with the cultivation containers. On the premise of guaranteeing that space and time are saved, the cost is low, and the utilization rate of resources is high, the utilization rate of the resources and labor productivity are increased, the cultivation period is shortened, and the production cost is lowered.

The invention provides a method for efficiently breeding tissue culture seedlings of bletilla striata and a planting method of the bletilla striata. The method for efficiently breeding the tissue culture seedlings of the bletilla striata comprises the following steps: seeding bletilla striata seeds into a seed germination culture medium; carrying out cultivation and germination for 30-40d; and transferring the germinated seeds into a seedling culture medium to cultivate for 3-4 months, wherein the seed germination culture medium comprises 1 / 2 modified MS culture medium, 30-40g / L of potato juice and 20-30g / L of cane sugar; the seedling culture medium comprises the modified MS culture medium, 30-40g / L of a potato juice, 60-70g / L of a banana juice, 20-30g / L of cane sugar and 3.8-4.5g / L of agar powder. The planting method of the bletilla striata comprises the following steps: transplanting the tissue culture seedlings of the bletilla striata into a farmland; carrying out water and fertilizer management after transplanting; and carrying out weed control and diseases and pests prevention and control. The methods are liquid culture medium methods, and only need to transfer once. Compared with a traditional solid culture method, the methods are simple to operate, small in pollution, low in cost, high survival rate and short in culture cycle.

The invention provides a high active induction culture method for lily bulbil which is characterized in that: through an induction culture of embryoid and tissue enrichment culture of embryoid tissue, a relative high rapid propagation velocity is obtained; then through an differentiation culture of bulb-lets and an intumescence culture of bulb-lets, transplanting bulb-lets of a circumferential diameter bigger than 3cm are obtained. The growth rate of the bulb-lets of the invention can reach more than one million grains per years which is ten times that of conventional methods. With a large number of small bulb-lets obtained, the ratio of bulb-lets with large specification and large grain size is increased. The invention optimizes the culture procedures of preferential lily seedball, shortens the propagation time of preferential lily seedball; furthermore, the field planting survival rate of bulb-lets is high and the production cost is low; the invention is suitable for the scale rapid propagation production of cut lily elite.

The invention discloses a detection method for obligate hydrocarbon oxidizing bacteria, belonging to the technical field of microorganism detection methods. The detection method comprises the following steps of: suspending and diluting a soil sample with a basic culture medium; performing short-term culturing in combination with a selective culture carbon source and an oxidation reduction indicating dye for 3-5 days; and computing the quantity of obligate hydrocarbon oxidizing bacteria in the soil sample according to the color change of an airtight culture tube through a computer program. A detection technology for obligate hydrocarbon oxidizing bacteria disclosed by the invention has the advantages of simple operating process, economic efficiency, practicability, quickly-obtained result in a short period of time, high specificity, capability of effectively shielding the interference of other bacteria on counting and simple and accurate counting process, and is suitable for large-scale industrial exploration measurement with a large sample quantity.

The invention discloses a tissue engineering double-layer skin and a preparation method thereof. The tissue engineering double-layer skin is characterized by being constructed by using fibroblast cells and epidermal cells as seed cells and an acellular amniotic membrane as a biological scaffold. The tissue engineering double-layer skin disclosed by the invention has the advantages of low cost, simple operation, wide sources and easy storage, and is a skin substitution having high transplantation efficiency. The invention also provides the preparation method of the tissue engineering double-layer skin.

The invention discloses a mixed culture material for cultivation of eryngii mushroom. The mixed culture material consists of the following raw materials in percentage by weight in drying states: 10-20% of weed tree sawdust, 35-40% of corncob, 10-20% of bagasse, 15-25% of wheat bran, 3-8% of corn meal, 2-7% of soybean meal, 1-5% of calcium carbonate and 1-5% of lime; the water content of the mixed culture material after pre-wetting is 65+ / -1%, and the pH value is 7-9. The invention also designs a method for bagging the mixed culture material for cultivation of eryngii mushroom. The mixed culture material for cultivation of eryngii mushroom and the bagging method of the mixed culture material, designed by the invention, can be used for ensuring the production speed and color and luster of eryngii mushroom, improving the nutritive values of eryngii mushroom, reducing the cost and improving the yield.

The present invention provides one method of culturing Northern Cordyceps in a airlift bioreactor in large scale and separating and extracting Cordyceps extract. Seed for fermentation is cultured in MS culture medium and then transferred to 20 L bioreactor for large scale culture. The culture liquid in the bioreactor is then separated and purified to obtain product with Cordyceps extract content up to 46.25 % and purity of 99 %. The process of the present invention has low production cost and no environmental pollution.