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Freshwater chlorella and application thereof in fixation of CO2 and production of microalgae oil

A technology of microalgae oil and chlorella, which is applied in microorganism-based methods, microorganisms, biofuels, etc., can solve the problem of high production cost of microalgae oil, and achieve the effects of good application potential, high yield and short growth cycle.

Inactive Publication Date: 2013-07-24
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a strain of freshwater chlorella, which can be coupled with high concentration of CO while producing high-yield microalgae oil 2 High-efficiency immobilization of microalgae to solve the problem of high production cost of microalgae oil

Method used

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  • Freshwater chlorella and application thereof in fixation of CO2 and production of microalgae oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Pick a single algae on a solid plate under sterile conditions and drop it into a small test tube containing 10ml of BG-11 sterilized culture solution, and culture it in a light incubator at 28°C and 3500lux light intensity, and grow to the end of the index. The inoculation ratio of 1:10 is used to expand the culture. The specific operation is: transfer the 10ml microalgae culture solution to the Erlenmeyer flask containing 100ml BG-11 sterilized culture solution, and place it in a light incubator at 28°C and 3500lux light intensity. When it grows to the end of the index, it will be the microalgae cell fluid that has been expanded and cultured.

[0032] Prepare 1 L of BG-11 medium in an airlift photosynthetic bioreactor, insert the expanded cultured microalgae cell liquid, so that the cell density in the medium is 6×10 7 pieces / ml. The initial pH is 8.0, the temperature is 28°C, and the light intensity is 3500lux, the air of 0.23vvm (CO 2 The content is 0.03% (CO2 volu...

Embodiment 2

[0035] According to the method of Example 1, the microalgae cell fluid was expanded and cultivated.

[0036] Prepare 1 L of BG-11 medium in an airlift photosynthetic bioreactor, insert the expanded cultured microalgae cell liquid, so that the cell density in the medium is 6×10 7 pieces / ml. The initial pH is 8.0, the temperature is 28°C, and the light intensity is 3500lux, the air of 0.23vvm (CO 2 Content is 5%), cultivated for 12 days.

[0037] The algal cells were collected by centrifugation, dried at a constant temperature of 60-80°C, and the dry weight of the algal powder was weighed and calculated. The biomass concentration of algae powder is 2250mg / L, the biomass production rate is 187mg / L / d, CO 2 The fixation efficiency is 343mg / L / d, and the oil content determined by the chloroform-methanol method is 30%. The fatty acid composition of oil is mainly short-chain fatty acids of C16 and C18.

Embodiment 3

[0039] According to the method of Example 1, the microalgae cell fluid was expanded and cultivated.

[0040] Prepare 1 L of BG-11 medium in an airlift photosynthetic bioreactor, insert the expanded cultured microalgae cell liquid, so that the cell density in the medium is 6×10 7 pieces / ml. The initial pH is 8.0, the temperature is 28°C, and the light intensity is 3500lux, the air of 0.23vvm (CO 2 Content is 20%), cultivated for 12 days.

[0041] The algal cells were collected by centrifugation, dried at a constant temperature of 60-80°C, and the dry weight of the algal powder was weighed and calculated. The biomass concentration of algae powder is 1520mg / L, the biomass production rate is 127mg / L / d, CO 2 The fixing efficiency is 240mg / L / d, and the oil content is 41% as determined by the chloroform-methanol method. The fatty acid composition of oil is mainly short-chain fatty acids of C16 and C18.

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Abstract

The invention provides freshwater chlorella sorokiniana XJ02 and application thereof in fixation of CO2 and production of microalgae oil. The preservation number of the chlorella is CCTCC (China Center For Type Culture Collection) NO: M2013104. The chlorella disclosed by the invention can be used for fixing 0.03-20% of CO2 at high efficiency, the CO2 fixation efficiency of chlorella is 130-343mg / L / d, the biomass (dry weight) concentration of the chlorella is 720-2250mg / L, the oil content of chlorella is 29.2-41%, the oil fatty acid of the chlorella is mainly composed of C16 and C18 short-chain fatty acids, and the chlorella is suitable for production of biodiesel. Compared with the reported microalgae, the chlorella has higher CO2 fixation efficiency and biomass yield; and the chlorella can be used for obtaining the microalgae oil at high yield by selecting an appropriate culture condition, thus the production cost of the microalgae oil can be greatly reduced. An excellent production algae is provided for high-efficient fixation of CO2 in the environment and preparation of biodiesel by the microalgae oil.

Description

technical field [0001] The invention belongs to the technical field of microalgae biology. It specifically involves a strain of freshwater chlorella Chlorella sorokiniana XJ02 and its ability to fix CO 2 and applications in the production of microalgal oils. Background technique [0002] Due to the extensive use of global fossil fuels, the "greenhouse effect" is increasing, and CO 2 As the gas that contributes the most to the "greenhouse effect", it accounts for more than 65% of the total effect. According to the World Meteorological Organization's 2011 Greenhouse Gas Bulletin, atmospheric CO 2 Concentrations have increased from about 280 ppmv pre-industrial to about 390 ppmv in 2010. Currently, atmospheric CO 2 The continuous increase of concentration has caused serious climate and environmental problems. Therefore, under the circumstances that the world is jointly facing the challenge of climate change and promoting sustainable economic and social development, CO 2 ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12P7/64C12R1/89
CPCY02E50/13Y02E50/10
Inventor 夏金兰金雪洁巩三强聂珍媛
Owner CENT SOUTH UNIV
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