34 results about "Biomass yield" patented technology

Provided in the present disclosure are a method for increasing the biomass, yield and nitrogen utilization rate of crops, and an iron-rich compound fertilizer. The method comprises: supplementing and applying an iron fertilizer to a crop, wherein the microelement iron in the iron fertilizer can significantly promote the absorption and assimilation of the mineral nutrition of a nitrogen-containing chemical fertilizer by the crop to which the fertilizer is applied, thereby producing a synergistic effect, and the amount of the iron fertilizer applied to the crop is 0.37-0.74 kg per Chinese acre. Moreover, by means of the method provided by the present disclosure, the tillering number and the nutrient absorption and utilization of the crop can be improved after fertilization according to a method for field crops and normal field management of crops, thereby effectively improving the biomass, yield and nitrogen utilization rate of the crop.

The invention provides a method for improving biomass, yield and nitrogen utilization rate of crops and an iron-rich compound fertilizer.The method comprises the steps that an iron fertilizer is supplemented and applied to the crops, so that the microelement iron in the iron fertilizer can remarkably promote absorption and assimilation of the applied crops on mineral nutrition of the nitrogenous fertilizer, and the synergistic effect is achieved; wherein the application amount of the iron fertilizer to the crops is 0.37-0.74 kg per mu. And by adopting the method provided by the invention, the tillering number and nutrition absorption and utilization of crops can be enhanced according to a fertilization method of field crops and normal crop field management, so that the biomass, the yield and the nitrogen utilization rate of the crops are effectively improved.

The invention relates to the technical field of plant genetic engineering, and provides application of Leymus chinensis LcCBP60b gene in regulation and control of plant tiller number and biological yield. It is found for the first time that the LcCBP60b gene can improve the tiller number and the biological yield of leymus chinensis, and the LcCBP60b gene has important guiding significance on cultivation of high-yield and high-quality leymus chinensis varieties; the invention also clarifies the molecular mechanism of the LcCBP60b gene in regulation and control of the tiller number and the biological yield of Leymus chinensis, further analyzes the molecular module for regulating and controlling the tiller number and the biological yield of plants, can enrich the corresponding molecular mechanism, and provides a new breeding direction for breeding high-yield and high-quality varieties.

The utility model discloses a photobioreactor, which relates to the technical field of reactors and comprises a support plate, a reaction container is fixed at the upper end of the support plate, an L-shaped support plate is fixed on the upper end face of the support plate and positioned on one side of the reaction container, one end of a horizontal section of the L-shaped support plate is connected with a connecting ring, and a U-shaped rod is fixed on the upper end face of the connecting ring. An annular lamp strip is fixed to the lower end face of the connecting ring, an electromagnetic valve is installed on the top of the side wall of the air supplementing pipe, and one end of the electromagnetic valve is connected with a connecting hose. According to the device, carbon dioxide is supplemented into the reaction container through the air supplementing pipe, favorable conditions are provided for photosynthesis of algae, the air supplementing pipe and the turning plate can be driven to move up and down through stretching and retracting of the telescopic air cylinder, a nutrient solution is stirred through the turning plate, nutrient substances are evenly distributed, and the utilization rate of the nutrient solution is improved. Therefore, the growth rate and the biomass yield of the microalgae are ensured.

The present invention relates to a transgenic plant having increased biomass, yield, and / or abiotic stress tolerance. In particular, the present invention relates to a transgenic plant or plant cell into which a nucleic acid molecule encoding an RNA demethylase FTO (fat mass associated with obesity) protein is introduced. The present disclosure also provides methods of producing plants having increased biomass, yield, and / or abiotic stress tolerance.

This invention discloses a method for cultivating Spirulina, relating to the field of microalgae biotechnology, aiming to overcome the shortcomings of existing technologies in terms of low Spirulina biomass yield and protein content. The method includes: in a first cultivation stage, inoculating Spirulina into a basal culture medium and conducting photoautotrophic cultivation at a light intensity of 5000-10000 lux until the optical density (OD560) reaches 0.6-0.8; in a second cultivation stage, reducing the light intensity to 1000-3000 lux and adding calcium alginate-encapsulated sodium bicarbonate microspheres, cultivating until the algal cell dry weight reaches 1.5-2.0 g / L; in a third cultivation stage, increasing the light intensity to 8000-12000 lux and adding sodium nitrate slow-release granules supported on anion exchange resin. This invention can achieve a synergistic increase in Spirulina biomass dry weight and protein content.

The present invention is directed towards a transgenic plant that has an increased biomass, yield and / or abiotic stress tolerance. In particular, the present invention is directed to a transgenic plant or plant cell in which a nucleic acid molecule encoding an RNA demethylase FTO (fat mass and obesity-associated) protein is introduced. Also provided by the disclosure are methods of producing the plants with increased biomass, yield and / or abiotic stress tolerance.

The application belongs to the field of optical functional composite material application, and discloses a preparation method of conjugated oligomer / gold nanocluster light energy conversion material. The preparation method is to synthesize conjugated oligomer material with main chain of fluorene and thiophene and side chain containing active groups, and to compound the conjugated oligomer material with gold nanocluster containing mercapto ligand through covalent connection to prepare conjugated oligomer / gold nanocluster compound. The preparation method is simple and easy to operate, and can effectively compound two kinds of semiconductor materials. The nanocomposite has a size of about 2 nanometers and has the ability to quickly enter photosynthetic organisms and structures. The conjugated oligomer / gold nanocluster heterojunction is cultured with photosynthetic organisms and structures, and under sunlight irradiation, high-efficiency photo-generated electrons are generated and quickly transferred to the electron transport chain of the photosynthetic organisms and structures, so as to improve the solar energy conversion efficiency, increase the biomass yield, and have practical application value in the field of energy conversion.

The present invention belongs to the technical field of high-ammonia nitrogen wastewater treatment. The present invention discloses a strain of Synechocystis S1 that can form a mixed algae film with green algae and its application. The present invention also discloses a method for treating high-ammonia nitrogen wastewater by using Synechocystis S1 and green algae to form a mixed algae film, the method comprising adding a mixed seed liquid of Synechocystis and green algae into a rotating biofilm reactor for film culture to form a mixed algae film, and then operating the rotating biofilm reactor to treat high-ammonia nitrogen wastewater. The method of the present invention can treat high-ammonia nitrogen wastewater with a concentration of 1000 mg / L, and has a high biomass yield and a good ammonia nitrogen removal effect. Algal cells can be recovered by scraping, overcoming the shortcomings of low light utilization, difficult harvesting, and inability to continuously process and operate in traditional suspension culture methods. Ammonia nitrogen in wastewater can be continuously and efficiently bioconverted while harvesting high-value microalgae biomass.

This invention proposes a bioecological process for utilizing waste from sugary drinks by producing highly nutritious proteins from single-celled organisms, as well as other molecules of various interest, while significantly reducing the pollutant load of this waste. The process involves the upstream separation of the returned products, which are highly concentrated in sugars. The first step involves preparing the musts for fermentation by degassing and adjusting the sugars to 20 g / L and the pH to 5. An enrichment matrix is ​​used for musts made from carbonated beverage waste. The culture is carried out in batch or semi-continuous mode at a temperature of 30 ± 2°C with an agitation speed of approximately 150 rpm and oxygenation to promote oxidative metabolism. The incorporation of nectars and juices into the fermentation musts of carbonated beverages at a rate of ≥ 50% ensures a significant improvement in biomass yields. The process guarantees almost complete depletion of the sugars initially present in the musts (≥ 95%), producing biomass with a protein content exceeding 50% on a dry matter basis, which can be used in animal feed, aquaculture, or cosmetics.

The invention relates to the technical field of biology, in particular to the technical field of formic acid biological utilization and efficient biological conversion. The invention provides a formic acid biotransformation engineering strain. The bacterial strain is obtained after a plasmid pED31-FFM and a plasmid pAD31-gcvTHP are introduced into escherichia coli, and the plasmid pED31-FF M is obtained after an ftfl-fchA-mtdA (FFM) gene is connected to a pED31 empty plasmid; the plasmid pA D31-gcvTHP is obtained by connecting a gcvTHP gene to a pAD31 empty plasmid, and the plasmid pA D31-gcvTHP can be obtained by connecting the gcvTHP gene to the pAD31 empty plasmid. According to the strain provided by the invention, under the initial concentration of 20-60mM formic acid, the multiplication time is within 5 hours, the biomass yield can reach 6g CDW / mol formic acid, the single-cell protein yield can reach 2.45-4.2 g / mol, and the international leading level of formic acid conversion and biomass is reached.

The invention discloses a method for producing biodiesel by using organic solid waste anaerobic fermentation liquor and microalgae, which comprises the following steps: inoculating microalgae into a culture medium for culture, and collecting algae cells; and adding the organic solid waste anaerobic fermentation liquor into the residual culture medium, inoculating microalgae for culture, and collecting new algae cells. According to the method, the organic solid waste anaerobic fermentation liquid is directly added into the residual culture medium, so that a new microalgae culture system can be quickly formed, the production process can be simplified, continuous culture of microalgae can be realized, the accumulation amount of microalgae biomass and the yield of biodiesel can be increased, the use amount of water can be remarkably reduced, and the production cost can be reduced. And addition of a new carbon source is avoided, so that the production cost is reduced. The method has the advantages of being simple in process, convenient to operate, low in cost, high in microalgae biomass yield, high in biodiesel yield, good in biodiesel quality and the like, is suitable for large-scale production of the biodiesel, and facilitates industrial application of the biodiesel.

Compositions and methods for generating transgenic plants with increased plant growth and improved multiple yield-related traits, which comprise vascular xylem tissue-targeting overexpression of transcription factors (TFs) involved in vascular xylem cell development, are provided. One of the methods includes introducing into a plant cell a nucleic acid construct consisting of a heterologous plant tissue-specific promoter which is operably linked to a polynucleotide encoding a polypeptide of subgroup 4 R2R3-MYB transcription factor that shares at least 60% sequence similarity with SEQ ID NO: 04, and obtaining transformed plant cells; obtaining transgenic plants expressing the polypeptide from the transformed plant cells; and selecting a transgenic plant from the transgenic plants that expresses the polypeptide. Expression of the polypeptide in the selected transgenic plant exhibits both increased biomass yield and lower insoluble lignin content, as compared to a non-transgenic or wild-type control plant of the same plant species.

The application discloses application of an NRT2.1 gene in improving drought resistance and / or high-temperature resistance of crops and belongs to the technical field of biotechnology. The application provides a gene NRT2.1 capable of regulating nitrogen utilization of crops, wherein a CDS region nucleotide sequence of the NRT2.1 gene is shown in SEQ ID NO. 1 or has at least 70 % homology with the sequence shown in SEQ ID NO. 1 and the coded protein is functionally equivalent. The function of the gene is verified by obtaining NRT2.1 gene overexpression plants through transgenic technology, and the NRT2.1 gene overexpression significantly improves the biomass, yield and nitrogen utilization efficiency of the plants under drought or high-temperature stress conditions. The application provides a candidate gene for crop new variety breeding and has potential application value in agricultural development.

The invention discloses a system and a method for improving oil yield and cadmium removal rate of microalgae under cadmium stress, and belongs to the technical field of biological engineering. According to the method, a mixotrophic culture mode is adopted, and algae species mixotrophic culture is carried out, so that algae cells rapidly grow in a short time and are cultured to a logarithmic growth expectation for later use; and applying cadmium stress by using the same mixotrophic mode, and simultaneously adding exogenous fulvic acid to induce microalgae to grow and accumulate grease. According to the method, a mixotrophic culture mode is adopted, under the stress of 0.2 mu M Cd, when the concentration of fulvic acid is 40mg / L, the biomass, biomass yield and grease yield of the microalgae are respectively improved by 20.22%, 20.32% and 26.55% compared with those of single cadmium treatment, and the cadmium removal rate is 100% and is improved by 16.6% compared with that of single separation treatment. According to the method, the biomass and grease yield of the microalgae can be improved, meanwhile, removal of cadmium is increased, and a new theoretical basis is provided for co-production of grease by treating cadmium-containing wastewater through the microalgae.

The utility model relates to microalgae cultivation technical field discloses a kind of microalgae outdoor cultivation integrated device, including the support of four columns, the top end of the support swing installation is self retracting sunshade mechanism, the self retracting sunshade mechanism includes self retracting sunshade net, driving assembly is installed on the support and drives self retracting sunshade net contraction, further including adjustable color acrylic plate installed on the support and located below self retracting sunshade net. Through the design of self retracting sunshade mechanism cooperation colorable acrylic plate, self retracting sunshade mechanism can be automatically adjusted according to real-time light intensity self retracting sunshade net's unfolding range, acrylic plate can show different color in green cell stage and red cell stress stage, and with self retracting sunshade net linkage control, realize the accurate control of spectrum, solve artificial operation hysteresis problem, achieve the core purpose of biomass yield, astaxanthin accumulation speed up, and reduce energy consumption.

The invention provides a microalgae carbon sequestration efficiency improving device with built-in optical fiber bundle-quantum dot light guide, and belongs to the field of biological carbon sequestration. A traditional external light source irradiates a microalgae photobioreactor through a spatial light path, and the problems that light cannot penetrate into algae liquid, and the wavelength is limited by the light source exist. According to the invention, the optical fiber bundle protected by the transparent light guide tube is placed in the microalgae photoreactor for light guide, so that the illumination depth is prolonged, and the illumination area is increased. In addition, a quantum dot solution can be introduced into the light guide tube, and light with a proper wavelength is introduced through the optical fiber to irradiate the quantum dot solution to pump and emit light. The quantum dot has the characteristics of wide absorption spectrum and narrow emission spectrum, can capture ultraviolet-blue-violet light which is difficult to utilize by microalgae chlorophyll, and emits light with new wavelength through fluorescence resonance energy transfer to irradiate microalgae. The built-in optical fiber bundle-quantum dot solution is introduced to guide light and emit light, so that the illumination depth of algae liquid can be prolonged, the illumination area can be increased, the microalgae carbon sequestration efficiency and the biomass yield can be improved, the wavelength can be regulated and controlled based on the fluorescence resonance energy transfer characteristic of quantum dots, the illumination requirements of different types of microalgae can be accurately matched, and the application prospect is wide. And the device adaptation range is expanded.

This invention discloses a method for producing dendrobium phenols from Dendrobium suspension protocorms. The method involves screening to obtain Dendrobium suspension protocorms with high dendrobium phenol content, followed by induction, subculture and proliferation, and continuous cultivation in a bioreactor. The culture products are then collected, and dendrobium phenols are extracted. This invention enables the industrial-scale production of dendrobium phenols, featuring high biomass yield, stable and controllable dendrobium phenol content, labor savings, and independence from seasonal and temporal influences. Furthermore, by using an optimized culture medium, the dendrobium phenol content in fresh Dendrobium products can be effectively increased.

The application discloses a method for treating dairy farm wastewater based on Synechococcus, Aspergillus and green algae. In the wastewater treatment method, Aspergillus is used in the first step, and can realize rapid conversion of organic matter in dairy farm wastewater. Synechococcus is used in the second step, and has strong treatment capacity for high-concentration wastewater in a dairy farm, and is named Synechococcus ZSL-NS1. On the basis of the first two steps, the biomass can be rapidly increased. Finally, the removal rate of pollutants in the dairy farm wastewater can reach more than 80%, and the biomass yield is increased by 2.3 times. In addition, Synechococcus ZSL-NS1 can also produce chlorophyll, protein, polysaccharide, oil and other biomasses, so that the purpose of recycling is achieved while the pollution is removed, and the method has application value.

This invention relates to the field of plant genetic engineering technology, and provides sheepgrass. LcCBP60b The application of genes in regulating plant tiller number and biomass yield. This invention is the first to discover... LcCBP60b Genes can increase the number of tillers and biomass of Leymus chinensis, which is of great guiding significance for breeding high-yield and high-quality Leymus chinensis varieties; this invention also clarifies that LcCBP60b Investigating the molecular mechanisms by which genes regulate tiller number and biomass of Leymus chinensis, and further elucidating the molecular modules that regulate plant tiller number and biomass, can enrich the corresponding molecular mechanisms and provide new breeding directions for selecting high-yield and high-quality varieties.

The invention provides a marker combination and a primer for identifying the SV variation site of the biomass yield of the pennisetum americanum, genetic typing is performed on the pennisetum americanum by combining a biomass yield result obtained by actual detection of the pennisetum americanum, and if the sequence is SEQ ID No: 1 (reference), the pennisetum americanum to be detected is a candidate germplasm with the characteristic of low biomass yield, and the to-be-detected pennisetum americanum is a candidate germplasm with the characteristic of low biomass yield. If the gene is SEQ ID No: 2 (variation), the pennisetum americanum to be detected is a candidate germplasm with the characteristic of high biomass yield. Based on the result, an identification result can be effectively, simply, conveniently and quickly provided for the biomass yield of the pennisetum alopecuroides germplasm in advance, and the accuracy of predicting the forage grass yield per mu by the pennisetum alopecuroides can be improved within a certain limit, so that the pennisetum alopecuroides materials or strains with high biomass yield sites can be quickly screened out for breeding.

This invention provides a marker combination and primers for identifying SV variant sites in *Napier grass* (a type of grass) to determine biomass yield. Combined with actual biomass yield data obtained from *Napier grass* testing, genotyping is performed. It is found that if the sequence is SEQ ID No:1 (reference), the tested *Napier grass* is a candidate germplasm with low biomass yield; if it is SEQ ID No:2 (variant), the tested *Napier grass* is a candidate germplasm with high biomass yield. Based on this result, the biomass yield of *Napier grass* germplasm can be identified quickly and easily in advance, improving the accuracy of forage yield prediction to a certain extent. This allows for the rapid screening of *Napier grass* materials or strains with high biomass yield sites for breeding.

The present invention relates to the technical field of rapid statistical analysis of silage corn biomass yield. The present invention discloses a method for rapid statistical analysis of silage corn biomass yield, comprising the following steps: step one, selection of harvesting time; step two, calculation of silage corn plant spacing and row spacing; step three, calculation of silage corn planting density; step four, calculation of silage corn single plant weight; and step five, calculation of silage corn per mu yield. The invention has the following beneficial effects: 1. It standardizes the statistical method of silage corn biomass yield, and provides scientific guidance for the quality and weight of silage corn feed for scientific breeding. 2. It improves the harvesting efficiency of silage corn, reduces the labor intensity of harvesting silage corn, and can weigh the entire harvested silage corn plant, thereby reducing the error in statistical analysis of silage corn biomass yield.

The invention discloses a method for measuring and controlling high-yield fucoxanthin and EPA (eicosapentaenoic acid) through diatom light fermentation by adding an organic acid coupling spectrum and application of the method. The method comprises the following steps: S1, inoculating diatom into a seed solution culture medium, and performing illumination culture to a logarithmic phase to obtain a diatom mixotrophic seed solution; and S2, inoculating the diatom mixotrophic seed solution into a light fermentation tank filled with a fermentation culture medium containing lactic acid, glycerol and a nitrogen source for light fermentation culture, and performing real-time monitoring and feedback control on intermittent material supplementation by using an online near infrared spectrum analyzer so as to improve the biomass yield of the diatom and the yield of high-value natural products. The method for on-line measurement and control of diatom light fermentation provided by the invention can be used for producing fucoxanthin and eicosapentaenoic acid.

Disclosed is a fertilizer composition containing dolomite and either or both of fulvic acid and / or a combination of polyhalite and calcium carbonate. Methods for improving the biomass yield of a plant by using the fertilizer composition are also disclosed.

The invention discloses a method for producing single-cell protein based on mixed culture of methane-oxidizing bacteria and aerobic zymophyte, which comprises the following steps: introducing mixed gas of methane and oxygen in a certain proportion into a culture medium containing the methane-oxidizing bacteria and the aerobic zymophyte, culturing the methane-oxidizing bacteria and the aerobic zymophyte, and converting the methane-oxidizing bacteria and the aerobic zymophyte into protein. According to the method, the methane-oxidizing bacteria and the aerobic zymophyte are co-cultured, and the aerobic zymophyte is introduced, so that the tolerance of the methane-oxidizing bacteria to exudates in the growth process of the methane-oxidizing bacteria is improved, and a higher biomass yield is achieved. The synergistic effect can effectively promote the growth of methane-oxidizing bacteria and improve the carbon fixation efficiency, and has a demonstrative effect on industrial large-scale production.

The invention relates to the technical field of photobioreactors, and discloses a suspension-biological membrane coupled microalgae culture reactor and an operation method thereof. The biological membrane culture area is located at the upper part of the culture box, a plurality of open carrier containers distributed along the height direction are arranged in the biological membrane culture area, and the upper surface of a bottom plate of each open carrier container is provided with a bulge structure for attaching a microalgae biological membrane containing microalgae cells; the suspension culture area is located at the lower part of the incubator, and a microporous aerator is arranged in the suspension culture area and is used for suspension culture of microalgae cells; the liquid circulation module is used for conveying the microalgae cells in the suspension culture area into the open carrier container, or circularly conveying a liquid culture medium without the microalgae cells between the suspension culture area and the open carrier container, and the problems that illumination is limited, mass transfer is insufficient, and harvesting is difficult in a traditional single culture mode are effectively solved; the space utilization rate, the biomass yield and the system stability of microalgae culture are improved.

The invention discloses polypeptide-assisted molybdenum disulfide nano-enzyme as well as a preparation method and application thereof, and relates to the technical field of polymer composite materials. The preparation method comprises the following steps: (1) dissolving a polypeptide polymer with a flexible main chain and a charged side chain in a solvent to prepare a polypeptide polymer solution; (2) mixing molybdenum source molecules, sulfur source molecules and a reducing agent in water to prepare a precursor solution; and (3) adding the polypeptide polymer solution obtained in the step (1) into the precursor solution obtained in the step (2), uniformly mixing, carrying out hydrothermal reaction, cooling, centrifuging, washing and drying to obtain the polypeptide-assisted molybdenum disulfide nano-enzyme. The polypeptide-assisted molybdenum disulfide nano-enzyme shows excellent succinate-like dehydrogenase and peroxidase catalytic activity, can effectively promote algae metabolism and increase the biomass yield, and has good practical value in the fields of biological catalysis, energy conversion and environmental protection.

Algae cultivation system is an increasingly important field due to their versatility and the high biomass yield produced. The continuous movement and Mixing of Algal Water will help Algal cell to get exposed to light source. Light availability the algal culture depends on the thickness of the system where algal culture is moving. The thin layers of algal culture also facilitate efficient gas exchange, ensuring that carbon dioxide is readily available for photosynthesis and oxygen is effectively removed, preventing oxygen accumulation that could inhibit growth. Algae requires few seconds of light exposure to start its photosynthetic activity. This light availability and culture movement has created huge difference between the laboratory and mass scale productivity. There is a need for a simple, energy-efficient system which makes algal cells to get maximum exposure to light.

The invention belongs to the technical field of microbial culture, and particularly relates to a special culture medium for a protoplast group of encircle coal velvet bacteria as well as a culture method and application of the culture medium. The special culture medium for the protoplast group of the encircle coal velvet bacteria comprises a carbon source, a nitrogen source, inorganic salt and growth factors, the carbon source is selected from one of fructose, maltose and cane sugar; the nitrogen source is selected from one of peptone and yeast powder; the inorganic salt is selected from one of monopotassium phosphate, magnesium sulfate and sodium chloride; the growth factor is vitamin B2 and / or vitamin C. The special culture medium provided by the invention can effectively promote the expansion and growth of the protoplast, can efficiently support the substance accumulation of the protoplast, improves the biomass yield, is more stable in migration behavior, and is beneficial to the uniform growth and resource utilization of the protoplast in the culture medium.