16 results about "Biomass yield" patented technology

The present invention relates to a transgenic plant having increased biomass, yield, and / or abiotic stress tolerance. In particular, the present invention relates to a transgenic plant or plant cell into which a nucleic acid molecule encoding an RNA demethylase FTO (fat mass associated with obesity) protein is introduced. The present disclosure also provides methods of producing plants having increased biomass, yield, and / or abiotic stress tolerance.

This invention discloses a method for cultivating Spirulina, relating to the field of microalgae biotechnology, aiming to overcome the shortcomings of existing technologies in terms of low Spirulina biomass yield and protein content. The method includes: in a first cultivation stage, inoculating Spirulina into a basal culture medium and conducting photoautotrophic cultivation at a light intensity of 5000-10000 lux until the optical density (OD560) reaches 0.6-0.8; in a second cultivation stage, reducing the light intensity to 1000-3000 lux and adding calcium alginate-encapsulated sodium bicarbonate microspheres, cultivating until the algal cell dry weight reaches 1.5-2.0 g / L; in a third cultivation stage, increasing the light intensity to 8000-12000 lux and adding sodium nitrate slow-release granules supported on anion exchange resin. This invention can achieve a synergistic increase in Spirulina biomass dry weight and protein content.

This invention proposes a bioecological process for utilizing waste from sugary drinks by producing highly nutritious proteins from single-celled organisms, as well as other molecules of various interest, while significantly reducing the pollutant load of this waste. The process involves the upstream separation of the returned products, which are highly concentrated in sugars. The first step involves preparing the musts for fermentation by degassing and adjusting the sugars to 20 g / L and the pH to 5. An enrichment matrix is ​​used for musts made from carbonated beverage waste. The culture is carried out in batch or semi-continuous mode at a temperature of 30 ± 2°C with an agitation speed of approximately 150 rpm and oxygenation to promote oxidative metabolism. The incorporation of nectars and juices into the fermentation musts of carbonated beverages at a rate of ≥ 50% ensures a significant improvement in biomass yields. The process guarantees almost complete depletion of the sugars initially present in the musts (≥ 95%), producing biomass with a protein content exceeding 50% on a dry matter basis, which can be used in animal feed, aquaculture, or cosmetics.

The invention relates to the technical field of biology, in particular to the technical field of formic acid biological utilization and efficient biological conversion. The invention provides a formic acid biotransformation engineering strain. The bacterial strain is obtained after a plasmid pED31-FFM and a plasmid pAD31-gcvTHP are introduced into escherichia coli, and the plasmid pED31-FF M is obtained after an ftfl-fchA-mtdA (FFM) gene is connected to a pED31 empty plasmid; the plasmid pA D31-gcvTHP is obtained by connecting a gcvTHP gene to a pAD31 empty plasmid, and the plasmid pA D31-gcvTHP can be obtained by connecting the gcvTHP gene to the pAD31 empty plasmid. According to the strain provided by the invention, under the initial concentration of 20-60mM formic acid, the multiplication time is within 5 hours, the biomass yield can reach 6g CDW / mol formic acid, the single-cell protein yield can reach 2.45-4.2 g / mol, and the international leading level of formic acid conversion and biomass is reached.

The application discloses application of an NRT2.1 gene in improving drought resistance and / or high-temperature resistance of crops and belongs to the technical field of biotechnology. The application provides a gene NRT2.1 capable of regulating nitrogen utilization of crops, wherein a CDS region nucleotide sequence of the NRT2.1 gene is shown in SEQ ID NO. 1 or has at least 70 % homology with the sequence shown in SEQ ID NO. 1 and the coded protein is functionally equivalent. The function of the gene is verified by obtaining NRT2.1 gene overexpression plants through transgenic technology, and the NRT2.1 gene overexpression significantly improves the biomass, yield and nitrogen utilization efficiency of the plants under drought or high-temperature stress conditions. The application provides a candidate gene for crop new variety breeding and has potential application value in agricultural development.

The utility model relates to microalgae cultivation technical field discloses a kind of microalgae outdoor cultivation integrated device, including the support of four columns, the top end of the support swing installation is self retracting sunshade mechanism, the self retracting sunshade mechanism includes self retracting sunshade net, driving assembly is installed on the support and drives self retracting sunshade net contraction, further including adjustable color acrylic plate installed on the support and located below self retracting sunshade net. Through the design of self retracting sunshade mechanism cooperation colorable acrylic plate, self retracting sunshade mechanism can be automatically adjusted according to real-time light intensity self retracting sunshade net's unfolding range, acrylic plate can show different color in green cell stage and red cell stress stage, and with self retracting sunshade net linkage control, realize the accurate control of spectrum, solve artificial operation hysteresis problem, achieve the core purpose of biomass yield, astaxanthin accumulation speed up, and reduce energy consumption.

The invention provides a microalgae carbon sequestration efficiency improving device with built-in optical fiber bundle-quantum dot light guide, and belongs to the field of biological carbon sequestration. A traditional external light source irradiates a microalgae photobioreactor through a spatial light path, and the problems that light cannot penetrate into algae liquid, and the wavelength is limited by the light source exist. According to the invention, the optical fiber bundle protected by the transparent light guide tube is placed in the microalgae photoreactor for light guide, so that the illumination depth is prolonged, and the illumination area is increased. In addition, a quantum dot solution can be introduced into the light guide tube, and light with a proper wavelength is introduced through the optical fiber to irradiate the quantum dot solution to pump and emit light. The quantum dot has the characteristics of wide absorption spectrum and narrow emission spectrum, can capture ultraviolet-blue-violet light which is difficult to utilize by microalgae chlorophyll, and emits light with new wavelength through fluorescence resonance energy transfer to irradiate microalgae. The built-in optical fiber bundle-quantum dot solution is introduced to guide light and emit light, so that the illumination depth of algae liquid can be prolonged, the illumination area can be increased, the microalgae carbon sequestration efficiency and the biomass yield can be improved, the wavelength can be regulated and controlled based on the fluorescence resonance energy transfer characteristic of quantum dots, the illumination requirements of different types of microalgae can be accurately matched, and the application prospect is wide. And the device adaptation range is expanded.

The application discloses a method for treating dairy farm wastewater based on Synechococcus, Aspergillus and green algae. In the wastewater treatment method, Aspergillus is used in the first step, and can realize rapid conversion of organic matter in dairy farm wastewater. Synechococcus is used in the second step, and has strong treatment capacity for high-concentration wastewater in a dairy farm, and is named Synechococcus ZSL-NS1. On the basis of the first two steps, the biomass can be rapidly increased. Finally, the removal rate of pollutants in the dairy farm wastewater can reach more than 80%, and the biomass yield is increased by 2.3 times. In addition, Synechococcus ZSL-NS1 can also produce chlorophyll, protein, polysaccharide, oil and other biomasses, so that the purpose of recycling is achieved while the pollution is removed, and the method has application value.

This invention relates to the field of plant genetic engineering technology, and provides sheepgrass. LcCBP60b The application of genes in regulating plant tiller number and biomass yield. This invention is the first to discover... LcCBP60b Genes can increase the number of tillers and biomass of Leymus chinensis, which is of great guiding significance for breeding high-yield and high-quality Leymus chinensis varieties; this invention also clarifies that LcCBP60b Investigating the molecular mechanisms by which genes regulate tiller number and biomass of Leymus chinensis, and further elucidating the molecular modules that regulate plant tiller number and biomass, can enrich the corresponding molecular mechanisms and provide new breeding directions for selecting high-yield and high-quality varieties.

This invention provides a marker combination and primers for identifying SV variant sites in *Napier grass* (a type of grass) to determine biomass yield. Combined with actual biomass yield data obtained from *Napier grass* testing, genotyping is performed. It is found that if the sequence is SEQ ID No:1 (reference), the tested *Napier grass* is a candidate germplasm with low biomass yield; if it is SEQ ID No:2 (variant), the tested *Napier grass* is a candidate germplasm with high biomass yield. Based on this result, the biomass yield of *Napier grass* germplasm can be identified quickly and easily in advance, improving the accuracy of forage yield prediction to a certain extent. This allows for the rapid screening of *Napier grass* materials or strains with high biomass yield sites for breeding.

The invention relates to the technical field of photobioreactors, and discloses a suspension-biological membrane coupled microalgae culture reactor and an operation method thereof. The biological membrane culture area is located at the upper part of the culture box, a plurality of open carrier containers distributed along the height direction are arranged in the biological membrane culture area, and the upper surface of a bottom plate of each open carrier container is provided with a bulge structure for attaching a microalgae biological membrane containing microalgae cells; the suspension culture area is located at the lower part of the incubator, and a microporous aerator is arranged in the suspension culture area and is used for suspension culture of microalgae cells; the liquid circulation module is used for conveying the microalgae cells in the suspension culture area into the open carrier container, or circularly conveying a liquid culture medium without the microalgae cells between the suspension culture area and the open carrier container, and the problems that illumination is limited, mass transfer is insufficient, and harvesting is difficult in a traditional single culture mode are effectively solved; the space utilization rate, the biomass yield and the system stability of microalgae culture are improved.

The invention discloses polypeptide-assisted molybdenum disulfide nano-enzyme as well as a preparation method and application thereof, and relates to the technical field of polymer composite materials. The preparation method comprises the following steps: (1) dissolving a polypeptide polymer with a flexible main chain and a charged side chain in a solvent to prepare a polypeptide polymer solution; (2) mixing molybdenum source molecules, sulfur source molecules and a reducing agent in water to prepare a precursor solution; and (3) adding the polypeptide polymer solution obtained in the step (1) into the precursor solution obtained in the step (2), uniformly mixing, carrying out hydrothermal reaction, cooling, centrifuging, washing and drying to obtain the polypeptide-assisted molybdenum disulfide nano-enzyme. The polypeptide-assisted molybdenum disulfide nano-enzyme shows excellent succinate-like dehydrogenase and peroxidase catalytic activity, can effectively promote algae metabolism and increase the biomass yield, and has good practical value in the fields of biological catalysis, energy conversion and environmental protection.

Algae cultivation system is an increasingly important field due to their versatility and the high biomass yield produced. The continuous movement and Mixing of Algal Water will help Algal cell to get exposed to light source. Light availability the algal culture depends on the thickness of the system where algal culture is moving. The thin layers of algal culture also facilitate efficient gas exchange, ensuring that carbon dioxide is readily available for photosynthesis and oxygen is effectively removed, preventing oxygen accumulation that could inhibit growth. Algae requires few seconds of light exposure to start its photosynthetic activity. This light availability and culture movement has created huge difference between the laboratory and mass scale productivity. There is a need for a simple, energy-efficient system which makes algal cells to get maximum exposure to light.

The invention belongs to the technical field of microbial culture, and particularly relates to a special culture medium for a protoplast group of encircle coal velvet bacteria as well as a culture method and application of the culture medium. The special culture medium for the protoplast group of the encircle coal velvet bacteria comprises a carbon source, a nitrogen source, inorganic salt and growth factors, the carbon source is selected from one of fructose, maltose and cane sugar; the nitrogen source is selected from one of peptone and yeast powder; the inorganic salt is selected from one of monopotassium phosphate, magnesium sulfate and sodium chloride; the growth factor is vitamin B2 and / or vitamin C. The special culture medium provided by the invention can effectively promote the expansion and growth of the protoplast, can efficiently support the substance accumulation of the protoplast, improves the biomass yield, is more stable in migration behavior, and is beneficial to the uniform growth and resource utilization of the protoplast in the culture medium.

Low moisture content at harvest is important since green biomass material is not suitable for many applications such as for combustion in power stations. It is unusual to find a Miscanthus variety that is both cold tolerant and / or vigorous but that dries down well in years with mild winters. A mild winter may reduce the amount of usable biomass that is obtained from Miscanthus plants, including from cold hardy varieties that will stay green and fail to dry down properly at the end of a season unless there is a heavy prolonged frost. To address this issue, the present description relates to the production of increased usable biomass yield that may be obtained at harvest from Miscanthus plants that are cold tolerant, vigorous, and which also have good dry down properties during mild winters. The present methods include plants and methods for regenerating plants from a cell, rhizome, or cutting of a Miscanthus sacchariflorus x Miscanthus sinensis cross, and then selecting one or more regenerated plants that produce increased usable biomass and / or increased carbon sequestration.