208 results about "Candidate Gene Association Study" patented technology

The invention belongs to the technical field of pepper planting, and discloses a pepper multi-branch gene CaBr1 linked KASP molecular marker as well as a primer, a kit and application thereof, the KASP molecular marker takes pepper S8 (zhangshuang) as a reference genome, and the single nucleotide polymorphism at the 103175415th basic group on the No.8 chromosome is subjected to basic group G-to-A replacement. A chromosome region closely linked with multiple branches of the pepper is obtained by using a BSA (Bovine Serum Albumin) population positioning method, a molecular marker is developed in a candidate region to determine a candidate gene CaBr1, a KASP molecular marker is screened according to base mutation of the candidate gene, and the marker is used for carrying out genotype identification on 90 randomly sampled single plants of an F2 population, and the coincidence rate reaches 100%. The result not only contributes to pepper branch phenotype identification and assistant breeding, but also lays a foundation for map-based cloning of the branch gene and analysis of a molecular mechanism of pepper plant type regulation.

The invention belongs to the technical field of plant genetic engineering, and discloses application of an OsSRO1c gene in controlling high-temperature resistance of rice. The OsSRO1c gene capable of improving the drought tolerance of rice is obtained through separation, cloning and functional verification, the nucleotide sequence of the OsSRO1c gene is as shown in SEQ ID NO.1, and the sequence of protein coded by the gene is as shown in SEQ ID NO.2. The gene OsSRO1c for controlling rice high-temperature response is cloned, CRISPR mutant phenotype identification is carried out on candidate genes, high-temperature stress phenotype identification in a seedling stage and an adult-plant stage shows that when a gene segment is deleted, the high-temperature resistance of rice is reduced, and when the expression quantity of the gene is increased, the high-temperature resistance of rice is improved, and the function and the application way of the gene are proved.

The present disclosure relates to a multi-phase method for determining a set of candidate genes. During a first phase, the method includes prompting a naïve language model with a plurality of prompts corresponding to a plurality of candidate genes to generate a set of initial scores indicative of each corresponding candidate gene's potential as a biomarker or therapeutic target. During a second phase, the method includes determining, for each candidate gene, a set of relevant documents from a curated document library. The method also includes prompting a further language model using the relevant documents to generate secondary scores. During a third phase, the method includes determining, for each candidate gene, at least one of: a decision classification, a recalibrated score, and a detailed scientific explanation. The method includes determining a final candidate set and conducting a multi-dimensional optimization analysis on each candidate gene of the final candidate set.

The invention belongs to the technical field of agricultural biology, and provides a soybean drought stress tolerance gene and application thereof, and the nucleotide sequence of the gene comprises a CDS sequence as shown in SEQ ID NO: 1; the nucleotide sequence has at least 90% of homology with the sequence shown in SEQ ID NO: 1 and is used for coding a protein with a drought stress response function; one or more nucleotides are substituted, deleted or inserted in the sequence as shown in SEQ ID NO: 1, and a nucleotide sequence of a protein with a drought stress response function is coded; according to the application disclosed by the invention, the direct association between the soybean gene Gm12g076200 and the drought stress tolerance is clarified, and the deletion of the soybean gene Gm12g076200 causes a soybean drought sensitive phenotype, so that the research blank of the gene on the stress response function is filled; the deletion of the gene can significantly reduce the tolerance of the soybean to drought stress, and the expression quantity is significantly increased under the drought stress, so that a key candidate gene is provided for research of a soybean drought-resistant molecular mechanism.

The invention discloses a QTL (Quantitative Trait Loci) influencing wheat plant height as well as a candidate gene TaMYB-A1 and application thereof. The QTL is QPh-7A-1534 and is located on a 7A chromosome, and the genetic interval of the QTL is 1528.3 cM to 1542.6 cM. A candidate gene exists on the QTL-QPh-7A-1534, specifically, the candidate gene is TaMYB-A1, and the nucleotide sequence of the candidate gene is as shown in SEQ ID No. 1. The candidate gene TaMYB-A1 is subjected to gene editing, and experiments prove that the TaMYB-A1 gene can influence the plant height of wheat, so that the genetic improvement and molecular breeding of the plant height character of wheat are facilitated, and the screening of wheat varieties can be accelerated to obtain dwarf wheat varieties.

The invention relates to the technical field of agricultural biology, in particular to a verticillium dahliae heat shock protein 90 ATP enzyme activating factor VdAHA1 as well as a coding gene and application of the verticillium dahliae heat shock protein 90 ATP enzyme activating factor VdAHA1. The invention provides a verticillium dahliae heat shock protein 90 ATP enzyme activating factor VdAHA1 related to high temperature resistance, drug resistance, environmental adaptability, ergosterol synthesis capability and pathogenicity of verticillium dahliae and a coding gene of the VdAHA1, and the VdAHA1 gene positively regulates the pathogenicity of verticillium dahliae and can be used as an important candidate gene for pathogenesis research of verticillium dahliae. The invention provides a theoretical basis for further understanding the pathogenic mechanism of pathogenic bacteria, also provides a new gene resource for verticillium dahliae pathogenic related genes, also provides a new idea for prevention and control of cotton verticillium wilt, and has a wide application prospect.

The invention provides an application of a rape RPM1 interaction protein BnaRIN4 in regulating and controlling the oil content of rape seeds. On the basis of whole genome association analysis and whole transcriptome association analysis, a candidate gene BnaRIN4 for regulating and controlling the oil content is predicted, a homozygous mutant material with deletion of the gene BnaRIN4 function is further created through a transgenic technology, and the gene plays an important role in negative regulation and control of the oil content of the rape seeds, so that the application of the gene in negative regulation and control of the oil content of the rape seeds is realized. By specifically knocking out or mutating the gene, a novel rape germplasm of which the oil content of the seeds is remarkably improved can be obtained. The invention discloses the biological function of the gene in regulating and controlling the oil content of rape seeds for the first time, and provides theoretical basis and technical support for molecular breeding of high-oil-content rape varieties.

The invention discloses a method and a system for assisting in inputting clinical information of rare diseases and analyzing gene mutation based on phenotypes. The method comprises the following steps: firstly, acquiring clinical information in voice, text and image forms of a patient through a multi-source data acquisition module, converting the clinical information into characters, and performing entity recognition and standardization processing to generate structured medical record data; secondly, extracting clinical phenotypes from the structured data; furthermore, a candidate gene list is obtained according to the gene-disease relationship, comprehensive scoring and sorting are carried out, and a concerned gene list is output. According to the method, efficient structured input and standardization of clinical information are realized, the accuracy and automation level of phenotype-gene matching are remarkably improved, the gene variation interpretation period is effectively shortened, and intelligent support is provided for precise diagnosis of genetic diseases.

The invention discloses a ZmRIPK2 protein and application of a coding gene thereof in plant drought resistance, the amino acid sequence of the ZmRIPK2 protein is shown as SEQ ID No.1, and the nucleotide sequence of the coding gene of the protein is shown as SEQ ID No.2. After the ZmRIPK2 gene is knocked out from a plant and drought stress treatment is carried out, the drought resistance of a mutant plant is improved compared with that of a control group. The invention further discloses a method for improving the drought resistance of the corn, the ZmRIPK2 gene is knocked out from a receptor plant, and the corn with high drought resistance is obtained. The invention verifies that the ZmRIPK2 protein and the coding gene thereof have a negative regulation function on the drought resistance of the corn, and an excellent candidate gene resource is provided for cultivating and improving a new variety of drought-resistant corn; according to the provided drought-resistant plant breeding method, the breeding process is more efficient, reliable, accurate and controllable, and the method has important theoretical and practical significance for improving crop resistance and accelerating the corn breeding process.

This invention provides a gene related to the glossy leaf trait in Rugao black cabbage and its application, belonging to the field of molecular genetics and breeding technology. Specifically, this invention identifies the gene controlling the glossy leaf trait in 'Rugao black cabbage', confirms that this trait is controlled by a single dominant gene, and successfully locates the candidate gene. BraA02g02617P This study confirmed the association between the gene mutation and the glossy leaf trait. The discovery of the new dominant gene controlling glossy leaves greatly improves the convenience of molecular breeding of cruciferous plants, especially Brassica species, and has promising application prospects.

The invention relates to the technical field of sugarcane smut resistance, and provides a molecular marking method for sugarcane smut resistance, which comprises a sample preparation unit, a double-sugarcane area inoculation identification unit, a whole genome association analysis unit, an SNP (Single Nucleotide Polymorphism) marker development unit, a marker verification unit and a result application unit, the output end of the sample preparation unit is connected with the input end of the double-sugarcane-area inoculation identification unit through a sample transmission channel, the double-sugarcane-area inoculation identification unit outputs phenotypic data to the whole-genome association analysis unit, and the whole-genome association analysis unit outputs remarkably associated SNP sites to the SNP marker development unit; and the SNP marker development unit outputs a specific primer to the marker verification unit. The molecular marker provided by the invention is based on the correlation between the candidate gene of the site and the known disease-resistant pathway of the plant, and the MYB transcription factor can improve or reduce the resistance level of the plant to pathogenic bacteria by regulating the synthesis of secondary metabolites, transduction of plant hormone signals, expression of defensive genes and the like.

The invention discloses a molecular marker closely linked with a thin-skinned muskmelon bacterial fruit blotch resistance gene and application thereof, and belongs to the field of muskmelon disease resistance identification. According to the method, a disease-resistant muskmelon strain and a susceptible muskmelon strain are subjected to interspecific hybridization, genetic analysis is carried out on BFB-resistant characters of muskmelons by utilizing an F2 group, a high-resistance gene pool and a high-susceptibility gene pool are constructed on the basis, and resistance candidate genes of the muskmelons are mined by utilizing a BSA-seq technology and a molecular marker technology. Research results can provide a research basis for disease resistance character gene mining and mechanism research, and the method has important scientific significance for screening and application of muskmelon disease-resistant germplasm resources. The molecular marker KASP988272 is developed according to the genotype difference between candidate gene parents, and the genotype identification accuracy of the molecular marker in an F2 group is as high as 80%. The developed molecular marker closely linked with the bacterial fruit blotch resistant gene is used for rapidly and efficiently identifying the bacterial fruit blotch resistant thin-skinned muskmelon variety, and a technical support is provided for selection of the bacterial fruit blotch resistant thin-skinned muskmelon variety.

The invention relates to the technical field of plant genetic engineering, and discloses application of a knockout rice OsWAK55 gene in improvement of drought resistance and salt resistance of rice. According to the invention, a candidate gene is subjected to CRISPR (clustered regularly interspaced short palindromic repeats) knockout, a mutant is subjected to phenotype identification and stress screening, the gene OsWAK55 for regulating and controlling rice drought and salt stress is cloned, a nucleoside sequence of the gene OsWAK55 is shown as SEQ ID NO: 1, and a protein sequence coded by the gene is shown as SEQ ID NO: 2. A stress experiment in the seedling stage of the mutant shows that after the gene is mutated, the drought resistance and the salt resistance of the rice are enhanced, and the enhancement of the drought resistance is related to the condition that the mutant seedling has a more developed and robust root system. Therefore, the gene OsWAK55 is knocked out, so that the improvement of the abiotic stress resistance of the rice is facilitated.

The invention belongs to the technical field of gene engineering, and particularly relates to an application of a BnaA02g04730D gene in improving the salt tolerance of oilseed rape. According to the invention, a high-salt-tolerance germplasm 'Xiangong saline-alkali oil No.1' is obtained by screening, and a key candidate gene BnaA02g04730D responding to salt stress is excavated by combining absolute quantitative transcriptomics analysis. Yeast stress phenotype identification and transgenic rape verification both prove that the gene positively regulates the salt tolerance of rape. Functional verification shows that under the stress of 0.24% NaCl, the SOD activity, the CAT activity and the POD activity of the overexpressed plant are remarkably improved compared with those of a wild type, and the MDA content is remarkably reduced. The invention preliminarily illustrates a molecular mechanism for endowing rape with salt tolerance by regulating and controlling an anti-oxidation defense system and a hormone signal channel. A new perspective is provided for analysis of a salt-tolerant molecular mechanism of the rape, and a gene resource with application value is also provided for molecular breeding of a new salt-tolerant variety.

The invention discloses a populus euphratica PeXTH7 gene and application of the populus euphratica PeXTH7 gene in improving drought resistance of plants, and belongs to the technical field of gene engineering. The CDS sequence of the populus euphratica PeXTH7 gene is disclosed for the first time and is as shown in SEQ ID NO.1, and the coded amino acid sequence is as shown in SEQ ID NO.2; the effect of the populus euphratica PeXTH7 gene in improving the drought resistance of the plant is disclosed for the first time, the root length and lateral root density of the plant are remarkably improved through overexpression of the populus euphratica PeXTH7 gene, and the drought resistance of the plant is improved by adjusting the root structure of the plant and promoting root development; the populus euphratica PeXTH7 gene provided by the invention can be used as a candidate gene for breeding the drought-resistant new variety of the plant, and is of great significance for cultivating the drought-resistant new variety of the plant by adopting a genetic engineering method, accelerating the breeding process and reducing the breeding workload.

The application discloses a method for quickly exploring quantitative trait genes of rice, and the method comprises the following steps: performing whole genome association analysis on a rice nested linkage mapping population and linkage analysis on each subpopulation, and exploring quantitative trait loci (QTL) which can be identified by both methods; comprehensively analyzing the function, variation and expression of candidate genes in the QTL; performing weighted scoring and sorting on the number of keyword matches in the annotation information of the candidate genes, the influence of the variation sites of the candidate genes on the gene function, the expression amount of the candidate genes in specific tissues and the like, and selecting three genes with the highest scores as the candidate genes of the target quantitative trait.

The invention relates to a corn ZmNF-YC3 gene and an SNP molecular marker and application thereof, and relates to the technical field of plant genetic engineering, the nucleotide sequence of the corn ZmNF-YC3 gene is as shown in SEQ ID NO.1, the amino acid of encoding protein is as shown in SEQ ID NO.2, and the corn ZmNF-YC3 gene can positively regulate the plant height and ear height of a corn plant. The corn ZmNF-YC3 gene has the advantages that by analyzing the expression profile of the corn ZmNF-YC3 gene, carrying out candidate gene correlation analysis on the plant height character of the corn ZmNF-YC3 gene and carrying out plant height and ear position height phenotype analysis on a ZmNF-YC3 gene knockout corn mutant material, the corn ZmNF-YC3 gene is proved to have a positive regulation effect on the plant height and ear position height character of corn; important gene resources and molecular markers are provided for creating novel dwarf density-tolerant maize germplasm by utilizing a molecular breeding means.

The application discloses a method for improving the resistance of citrus to Huangyanbing by using ClPsaN gene, comprising the following steps: (1) cloning a VIGS fragment of the citrus ClPsaN gene; (2) constructing two VIGS expression vectors of TRV and CLBV; and (3) transforming the VIGS expression vector into citrus to obtain VIGS plants in which the citrus ClPsaN gene is silenced. According to the method, the VIGS vector of the citrus ClPsaN gene is constructed, then the vector is transformed into citrus, and the obtained citrus plants can obviously show resistance to Huangyanbing. The titer of Huangyanbing, i.e., Huangyanbing yellow vein-illness virus, can be reduced by 22-34%, and the accumulation amount of virus protein can be reduced by 35-50%, so that the incidence of Huangyanbing is significantly reduced. In addition, the TRV silencing of the citrus ClPsaN gene does not affect the phenotype of the citrus plants, and the citrus ClPsaN gene can be used as a candidate gene to be used in the breeding of citrus plants resistant to Huangyanbing by using VIGS silencing, RNA interference and gene editing technologies in cooperation with multiple genes of the citrus plants resistant to or susceptible to Huangyanbing.

This invention discloses Chinese kale BoaFLZ35 The application of genes in promoting vegetative growth of plants. This invention utilizes a gene-based approach to construct a *Gynostemma pentaphyllum* plant. BoaFLZ35 Heterologous overexpression of the gene in Arabidopsis thaliana was used to observe and analyze its effects on vegetative growth traits such as root elongation and rosette leaf morphology in transgenic plants. The results showed that heterologous overexpression... BoaFLZ35 This gene can significantly increase root cell length, thereby promoting the elongation of the Arabidopsis taproot; simultaneously, it can also increase leaf cell volume, resulting in significantly larger rosette leaves in transgenic plants. Therefore, it is evident that... BoaFLZ35 This is an important gene in the FLZ family of plants that positively regulates vegetative growth. This invention provides new insights into the function of FLZ family genes in the genus Brassica and also provides important candidate genes for high-yield molecular breeding of Brassica vegetables such as Chinese kale.

The application discloses a gene data processing method and device, computer equipment and a storage medium, and belongs to the technical field of computers. Through a gene function query request of a to-be-tested gene, the application can call a gene association model corresponding to a cell type to which the to-be-tested gene belongs, mine a nonlinear association degree between the to-be-tested gene and a known candidate gene, and use the candidate gene with a higher nonlinear association degree to label function annotation information of the to-be-tested gene. The way of calling the gene association model to extract the nonlinear relationship is completely different from the way of extracting the linear relationship in traditional statistics, can deeply mine the candidate gene with a higher similarity to the to-be-tested gene, and the similarity is not a linear similarity but an implicit nonlinear similarity, so that the accuracy of the gene data processing process is greatly improved.

The application provides a method for identifying potato drought-resistant genes using hyperspectral, and relates to the technical field of crop drought-resistant gene identification, and comprises the following steps: setting a drought group and a control group, synchronously acquiring plant canopy hyperspectral images and transcriptome data and establishing a correlation data set; extracting spectral features, screening drought response candidate genes; calculating the correlation coefficient of each candidate gene and the spectral features based on Spearman correlation analysis, and determining the primary gene set according to whether the average value of a plurality of positions after the absolute value is sorted exceeds a threshold value; constructing a random forest regression model with the spectral features as input and the expression amount of the primary gene as output, predicting the expression amount through a verification sample, and screening target drought-resistant genes according to the coefficient of determination and the prediction error; constructing a target gene overexpression plant, measuring the relative change rate of physiological indexes and the change multiple of gene expression amount under drought, calculating a comprehensive function verification index, and determining the gene as a drought-resistant gene if the index exceeds a threshold value.

This invention discloses a rice OsRbohB protein, its encoding gene, and its applications. The amino acid sequence of the OsRbohB protein is shown in SEQ ID NO.2, and the nucleotide sequence of its encoding gene is shown in SEQ ID NO.1. This invention provides a rice transformation overexpression vector that overexpresses the OsRbohB gene using the ZmUbi promoter. The plant height, number of grains per panicle, and grain length of the overexpressed plants were significantly increased. This invention is the first to demonstrate that rice OsRbohB is a functional gene regulating rice plant height, number of grains per panicle, and grain length. The cloning and biological function verification of this gene have important reference value for the study of the molecular mechanisms regulating important agronomic traits in rice. Since the above traits are important traits related to rice plant type and yield, the functional analysis of OsRbohB in this invention provides a new candidate gene for rice design breeding and has great potential application value.

The application discloses an InDel molecular marker related to peanut crown rot resistance and application, and belongs to the field of molecular biology.The polymorphism of the InDel molecular marker on an A01 chromosome is that a 177 bp nucleotide sequence exists or is lost, and the nucleotide sequence is shown as SEQ ID NO.5.The molecular marker is based on a resistance major gene qCRA01 The co-dominant marker developed from the candidate gene is closely related to the resistance trait, and rapid resistance identification and screening of peanut breeding materials and germplasm resources can be realized.The application provides a reliable tool for peanut crown rot resistance molecular marker assisted breeding. Early selection is carried out by using the molecular marker, the workload of field identification can be greatly reduced, the introduction and aggregation of disease resistance genes to excellent backgrounds can be accelerated, the breeding period is shortened, and the breeding efficiency is improved, and therefore the application has important application value for cultivating high-yield, high-quality and disease-resistant peanut new varieties.

The invention belongs to the field of molecular biology of forestry insects, and particularly relates to a fluorescent quantitative reference gene of different gender adults of yellow shield elephant, as well as a primer and application of the fluorescent quantitative reference gene. According to the method, 8 candidate genes commonly used as reference genes are referenced and selected according to transcriptome sequencing data of the hedychium aureum, gene sequences of the 8 reference genes are identified, the stability of the candidate reference genes is evaluated through five algorithms, and the fluorescent quantitative reference genes suitable for the hedychium aureum adults with different genders are obtained. And a real-time fluorescent quantitative PCR (Polymerase Chain Reaction) primer of the candidate reference gene is designed. The detection primer provided by the invention is strong in specificity and high in amplification efficiency, fills up the current situation that no reference gene exists under adults with different genders of the hedychium aureum, provides a reliable analysis basis for expression quantification of related functional genes under the adults with different genders of the hedychium aureum, and has a good application prospect. A necessary earlier-stage foundation is laid for analysis of subsequent molecular mechanisms and mining of key functional genes, and repeatability, stability and reliability of functional gene research can be improved at the same time.

The application provides a corn breeding method based on MAS technology, comprising the following steps: marking existing corn low-temperature-tolerant quantitative trait locus information to a reference map, obtaining corn low-temperature-tolerant consistent quantitative trait loci through Meta analysis on the marked reference map; obtaining corn germination stage low-temperature-tolerant quantitative trait locus information based on the strongest low-temperature-tolerant corn inbred line and the weakest low-temperature-tolerant corn inbred line; determining corn germination stage low-temperature-tolerant trait candidate region mining response low-temperature significant difference candidate genes based on the consistent quantitative trait locus information and the corn germination stage low-temperature-tolerant quantitative trait locus information; and performing corn breeding according to the candidate genes. The corn low-temperature-tolerant quantitative trait locus information of a preset quantity is integrated, the relationship between the corn low-temperature-tolerant related quantitative trait locus effect and position is evaluated, the molecular marker technology applied to the MAS technology in the development process of the corresponding section is provided with powerful help, and the MAS assisted breeding efficiency is improved.

The present invention provides a method, apparatus, terminal, and medium for screening gene mutation points based on clinical phenotypes. The method includes: identifying candidate genes based on gene detection results, clinical text to be analyzed, and a multi-source database; calculating the basic weights of a first pathway; extracting candidate mutation sites within the genomic regions of the candidate genes and obtaining external evidence corresponding to the candidate mutation sites; performing phenotypic extraction on the clinical text to be analyzed to obtain a structured phenotype; inputting the structured phenotype, contextual information of the candidate mutation sites, and external evidence into a trained mutation site screening model to obtain intermediate semantic analysis results; determining the basic weights of a second pathway based on the intermediate semantic analysis results; obtaining a comprehensive weight for each candidate mutation site based on the basic weights of the first and second pathways; and screening the candidate mutation sites based on the comprehensive weights to obtain screening results. This application can perform site-level screening, improving the accuracy of gene mutation point screening.

The invention discloses screening and application of a fluorescent quantitative reference gene in a root and stem development process of iris vetiveria, and belongs to the technical field of plant genetic engineering. By constructing a strict reference gene evaluation and screening system, the reference gene with the most stable expression in different stages of root and stem development of iris vetiveria is screened out from eight candidate genes for the first time, errors in samples and between samples caused by internal reference selection are remarkably reduced, accurate correction and standardization of the expression level of a target gene are achieved, and the accuracy of the reference gene is improved. And reliable technical guarantee is provided for subsequent large-scale accurate quantitative analysis of irisone synthesis related functional genes.

The invention discloses a gene function prediction method based on semantic correspondence of a regulatory region. The method comprises the following steps: firstly, constructing an inter-species regulation semantic correspondence relationship data set, constructing an artificial intelligence model structure, then, constructing a cross-species semantic correspondence network, and finally, carrying out function annotation on a target gene or identifying a candidate gene with a specific function in a target species. The accuracy of the PhytoBabel model constructed by the method is obviously higher than that of other model structures. By utilizing the method disclosed by the invention, the genes ZmERF104 and ZmGRF16 for promoting the regeneration of the corn somatic embryos and the gene ZmNAC17 for inhibiting the regeneration of the somatic embryos, which cannot be found by the traditional method, are successfully identified.

The invention discloses an SNP marker related to oyster ERK phosphorylation level and high-temperature resistance and application of the SNP marker, and belongs to the technical field of genetic engineering and genetic breeding. The nucleotide sequence of the SNP marker is as shown in SEQ ID NO.1, and A / G mutation exists at the 1188th site of the sequence. The ERK phosphorylation level and high-temperature resistance of an oyster individual of which the mutation site genotype of the SNP marker is GG are obviously higher than those of an oyster individual of AA and AG. Based on the molecular basis that the ERK phosphorylation level of crassostrea gigas and the ERK phosphorylation level of Fujian crassostrea gigas are remarkably different under the condition of heat stress, the key SNP marker for regulating ERK differential heat activation and the candidate gene of the key SNP marker are successfully screened out by systematically analyzing the upstream regulation pathway and combining whole genome association analysis. The marker has stable hereditary characteristics, can realize high-throughput and no-damage genetic typing of high-temperature-resistant oysters, and can be directly applied to assisted breeding.

The application relates to the technical field of biological information, and discloses a plant stress relief key gene tracing method and system based on transcriptome data. The method comprises the following steps: performing high-throughput transcriptome sequencing on plant leaves of four treatment groups, obtaining clean transcriptome data through quality filtering; obtaining a whole genome expression matrix through transcript assembly and redundancy removal processing, performing intersection screening on stress induction up-regulated genes and relief agent silencing down-regulated genes, and obtaining a stress induction-relief agent silencing candidate gene set; constructing a stress induction co-expression topology network by taking the candidate gene set as a silencing annotation basis, calculating a weighted neighbor silencing rate to obtain a topology feature matrix; training a selective silencing probability prediction model through a logistic regression model by taking the topology feature matrix and the candidate gene set, and performing channel enrichment calculation by taking the silencing probability as a weight to screen and obtain a core tracing gene set. The application improves the biological credibility of key gene tracing results and the cross-scene adaptability of the method.