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Method for obtaining capsicum phytophthora resistance candidate gene and molecular marker, and application

A technology of pepper blight and candidate genes, applied in the direction of DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of restricting the breeding process of pepper blight-resistant varieties, QTL cannot be mutually verified, and the effectiveness and versatility are not high. Achieve the effects of improving resistance and quality, satisfying the acceleration of replacement, and shortening the selection process

Inactive Publication Date: 2015-04-29
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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Problems solved by technology

However, due to the different resistance and susceptibility materials and Phytophthora strains used by different researchers, these QTLs obtained cannot be mutually corroborated, and the related molecular markers developed based on QTLs and disease resistance-related genes are due to insufficient linkage and The characteristics of the marker itself make its effectiveness and versatility not high, which restricts the breeding process of pepper blight-resistant varieties to a certain extent

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  • Method for obtaining capsicum phytophthora resistance candidate gene and molecular marker, and application
  • Method for obtaining capsicum phytophthora resistance candidate gene and molecular marker, and application
  • Method for obtaining capsicum phytophthora resistance candidate gene and molecular marker, and application

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Embodiment 1

[0024] Pepper (Capsicum spp.) is one of the most important vegetable crops in the world and has become the second largest vegetable crop in China. At present, pepper is one of the important economic crops in protected cultivation in my country, and has become a pillar industry for increasing farmers' income in the local planting industry in key production areas. However, many unfavorable factors in production have restricted the development of pepper planting industry. At present, blight (Phytophthora capsici Leon) has become the most important disease in pepper production in my country, with a wide distribution and serious damage.

[0025] Due to the prevalence of pepper blight and the seriousness of the disease caused by it, and the variety of transmission routes, the occurrence of the disease is often explosive, resulting in no effective methods for preventing and controlling the disease in production. In order to effectively prevent the occurrence of pepper blight, select...

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Abstract

The invention relates to a method for obtaining a capsicum phytophthora resistance candidate gene and a molecular marker, and application. The method is used for obtaining the capsicum phytophthora resistance candidate gene by utilizing capsicum phytophthora transcriptome and whole-genome sequencing data information, differentially-expressed gene identification, bioinformatics analysis, molecular marker development and phytophthora inoculation identification and belongs to the technical field of capsicum biology. The method comprises the following steps: sequencing a phytophthora resistant and susceptible gene pool transcriptome obtained after phytophthora inoculation of an F2 population constructed by capsicum highly-resistant and highly-susceptible phytophthora materials, performing expression analysis and functional annotation on differential genes, extracting DNAs (Desoxvribose Nucleic Acid) of a capsicum phytophthora highly-resistant and highly-susceptible phytophthora material genome, performing primer design and PCR (Polymerase Chain Reaction) amplification, performing sequence difference analysis and SNP site identification, performing SNP specific primer design and validity verification, and performing other steps to efficiently obtain the capsicum phytophthora resistance candidate gene and the molecular marker. According to the method, the capsicum phytophthora resistance candidate gene can be accurately identified, and the effective molecular marker can be developed.

Description

technical field [0001] The invention belongs to the field of capsicum biotechnology, and specifically relates to a method and application for obtaining capsicum blight resistance candidate genes and molecular markers, mainly through capsicum blight transcriptome sequencing, differential gene expression analysis and functional annotation, genomic DNA extraction, and PCR amplification , Amplified sequence difference analysis and SNP site identification, SNP primer design and validity verification, and then obtain pepper blight resistance candidate genes and molecular markers. This method is suitable for pepper blight resistance gene identification and molecular marker-assisted selection breeding. Background technique [0002] Pepper (Capsicum spp.) is one of the most important vegetable crops in the world. It has many varieties, high yield, rich nutrition and wide application, and plays an important role in people's daily life. In China, after decades of development, the chili...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 刁卫平王述彬刘金兵潘宝贵戈伟郭广君高长洲
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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