76 results about "Gene screening" patented technology

The invention discloses a spatial transcriptome data analysis method based on artificial intelligence, and belongs to the technical field of spatial transcriptomics data analysis. Firstly, self-adaptive normalization and hypervariant gene screening preprocessing are carried out on original gene expression data; then constructing a hierarchical map integrating spatial proximity and transcription similarity, and ensuring the connectivity and robustness of the map through a dynamic radius pruning and neighborhood inheritance strategy; dividing positive and negative sample sets based on the atlas, and inputting a type modulation contrast graph auto-encoder for training; and finally, spatial domain identification and downstream function analysis are completed based on the low-dimensional potential representation or reconstructed gene expression matrix output by the model. The method effectively improves the accuracy and stability of spatial domain recognition, adapts to multi-technology-source data, enhances the biological interpretability of model output, and can be widely applied to biomedical scenes such as tumor microenvironment analysis and organ development research.

The invention discloses a carbapenemase OXA-58-like positive plasmid and a host bacterium propagation risk assessment method thereof, which are used for carrying out integrated assessment on the plasmid and the host bacterium in three dimensions of drug resistance, pathogenic potential and propagation potential, and carrying out comprehensive risk grading and classification by taking the OXA-58-like positive plasmid and the host bacterium thereof as a plasmid-host complex. The invention develops a set of OXA-58-like gene screening and systematic analysis process based on a public plasmid database aiming at the defects that the existing research of the OXA-58-like gene is mostly limited to a single species or a local sample and a standardized automatic analysis method is lacked. According to the method, all OXA-58-like gene positive plasmids in a database can be subjected to unified and efficient drug-resistant gene mining, the association rule between the drug-resistant genes and plasmid pedigree, host strains and geographical distribution is systematically disclosed, and important technical support is provided for drug resistance propagation monitoring and risk assessment.

The invention discloses an adversity high-photosynthetic-efficiency transcription factor screening method based on deep learning, and relates to the technical field of biological information analys.The method comprises the steps that rice multi-modal stress response data is obtained and preprocessed, and preprocessed gene expression data is obtained; carrying out differential expression gene screening and co-expression network analysis on the preprocessed gene expression data, extracting multi-modal features, and fusing the multi-modal features to generate a multi-modal input feature matrix; constructing a double-layer deep learning model, training the double-layer deep learning model by using the multi-modal input feature matrix, and respectively outputting a regulation and control relationship matrix of transcription factors and target genes and a regulation and control relationship matrix of transcription factors and target pathways; and according to an output result, calculating a comprehensive score of each transcription factor through a multi-dimensional scoring system, and screening out the stress high-photosynthetic-efficiency transcription factor according to a predetermined screening standard.

This invention relates to soybean pod-resistant trait loci. qPD08‑1 This invention relates to the soybean pod-bomb resistance gene locus and its applications, belonging to the field of plant molecular biotechnology. qPD08‑1 Located in soybeans Glyma The reference genome of .Wm82.a2.v1, located between 35983663 and 36106921 bp on chromosome 8, has a total physical interval length of 123.258 kb. The molecular marker detection method of this invention can be used for identification of soybean pod-resistant germplasm resources, screening and functional studies of pod-resistant genes, marker-assisted selection breeding for pod-resistant soybeans, and natural resource applications. It features convenient detection, stable amplification products, and high specificity, helping to broaden the sources of superior breeding parents and improve the efficiency of soybean breeding selection.

The invention belongs to the technical field of gene engineering, and discloses application of an F-box protein gene OsFBX235 in improvement of bacterial blight resistance. The invention specifically discloses an application of knocking out OsFBX235 gene in improving the bacterial blight resistance of rice or cultivating a bacterial blight resistant rice variety. The F-box protein coding gene OsFBX235 is separated and cloned from rice, and the gene is proved to participate in the defensive reaction of the rice to the bacterial blight for the first time through functional analysis, and is an important negative regulation factor for regulating the resistance of the rice bacterial blight. The gene OsFBX235 is knocked out through target gene screening in combination with a CRISPR / Cas9 technology, and the importance of the gene OsFBX235 on resistance regulation and control of the rice bacterial blight is proved. Meanwhile, knockout of the gene can be used for creating rice germplasm resources with high bacterial leaf blight resistance and can be applied to breeding of new varieties with the bacterial leaf blight resistance of the rice.

This invention belongs to the field of bioinformatics and relates to a single-cell trajectory inference method based on adaptive feature selection. First, an initial gene expression matrix is ​​obtained through data preprocessing and screening for highly variable genes. Second, a two-dimensional evaluation strategy is employed to calculate the scores of highly variable genes with gene expression variability and the trajectory importance score related to differentiation trajectories. Then, a dynamic weight fusion mechanism is introduced, adaptively adjusting the fusion weights of the two scores based on performance feedback, and highlighting key genes through nonlinear enhancement. Next, an intelligent inflection point detection algorithm adaptively determines the optimal number of features. Finally, trajectory inference is performed based on a variational autoencoder model reconstructed from feature subsets, and a performance-driven feature selection closed loop is formed through multiple rounds of iterative optimization. This invention achieves high-precision, adaptive single-cell trajectory inference, solving the technical problems of single feature selection and fixed weights in traditional methods.

The utility model relates to the field of medical detection, provides a sample storage and treatment device for gene detection, and aims to solve the problems of inconvenience in storage, easiness in omission and risk of pollution of DNA (deoxyribonucleic acid) samples during treatment in the prior art, and the sample storage and treatment device comprises a shell, a puncher, a punching die and a storage positioning mechanism, the punching die is arranged on the shell; the storing and positioning mechanism is detachably connected to the punching die and used for storing and positioning the dried blood spot collection cards during punching. The storage positioning mechanism can store a plurality of dried blood spot collection cards after sampling and can also perform positioning; when the blood spot needs to be punched and cut out, the storage positioning mechanism is directly installed on the shell, and the puncher is used for punching. And through the matched design of the storage positioning mechanism and the punching die, the dried blood spot collection cards do not need to be sequentially taken out for punching during sample punching treatment, pollution to dried blood spot collection card samples for Rh blood group gene detection is effectively reduced, and more convenience is achieved. The method is especially suitable for large-scale Rh blood group gene screening.

The application provides an analysis method, device and equipment based on single-cell transcriptome sequencing data, which comprises the following steps: performing quality control, downstream analysis and visual display on single-cell transcriptome sequencing expression quantitative data, performing cell filtering by using Grubbs test method to obtain effective single-cell transcriptome sequencing quantitative data, performing initialization clustering analysis on the data to obtain single-cell subgroup classification results; performing screening on the single-cell subgroup classification results to obtain target single-cell subgroups, and performing re-clustering analysis to obtain single-cell sub-subgroup classification results; performing significant difference gene screening analysis on the single-cell transcriptome sequencing quantitative data between single-cell subgroups; and performing regression analysis based on characteristic genes of cell cycles to predict cell division periods corresponding to different cell types. The application effectively solves the technical complex problems of existing single-cell transcriptome sequencing quantitative data analysis, makes data analysis more simple and reliable, and reduces the difficulty of data analysis.

The invention discloses a bacterial gene editing tool based on an Ago2 and UvrD protein co-expression system and application. The bacterial gene editing tool comprises a first plasmid and a second plasmid, wherein the first plasmid comprises an Ago2 expression cassette and left and right homologous arms of a target gene, and the second plasmid comprises a UvrD expression cassette. According to the invention, the two plasmids are co-transformed into bacteria to obtain a strain without a target gene related sequence. The method is easy to operate, wide in application, free of potential off-target effect, high in knockout efficiency, free of resistance gene selection markers and suitable for bacteria which are difficult to edit or low in editing efficiency through a conventional gene editing method, and an excellent tool is provided for research and development of genetic engineering vaccines.

The invention belongs to the technical field of biological medicine and molecular biology, and provides a hypertrophic cardiomyopathy virulence gene MYH7c.794Cgt; the invention relates to T (p.Thr265Ile) and an application thereof. The MYH7 gene mutation is located on the ninth exon, the 794th base is mutated from C to T, namely ACC is mutated to ATC, and the 265th amino acid in the coded amino acid sequence is mutated from threonine to isoleucine. The mutation induces cardiac hypertrophy by disrupting energy metabolism-this defect occurs prior to the occurrence of systolic dysfunction. Along with increasingly prominent status of precision medicine in cardiovascular treatment, a treatment strategy aiming at an upstream pathological process (such as energy homeostasis and mitochondrial dysfunction) provides a way with a wide prospect for preventing and treating MYH7-related hypertrophic cardiomyopathy. MYH7 gene screening has important values in the aspects of promoting early diagnosis, guiding timely treatment intervention and realizing risk-based prevention and management.

The invention discloses a homologous recombination system, a transposition system, a fruit fly animal gene high-throughput screening method and application. The screening method comprises the following steps: firstly, separating and establishing a stable cell line from a fruit fly animal; then co-transfecting a CRISPR / Cas9 mediated homologous recombination system, and obtaining a cell line for stably expressing Cas9 protein through puromycin screening and a limited dilution method; the sgRNAs plasmid library constructed by combining a PiggyBac transposon system can realize efficient screening and functional analysis of a target gene in the Cas9 cell line. The high-throughput screening method provided by the invention is widely applicable to gene function research and target gene screening, is simple in preparation process and convenient to operate, has good universality, and provides effective technical support and solution for related fields.

The application relates to a cancer driver gene explainable identification method based on trust calibration and prototype learning, and relates to the technical field of biological information identification. A gene graph is constructed by fusing a protein interaction network and gene multi-omics characteristics, and part of nodes are labeled. Label-aware message passing is performed through a trust calibration encoder, the neighborhood is split into a labeled part and a non-labeled part for independent calibration, and node embedding is adaptively fused. An angle margin prototype classifier is used to construct a class prototype on a hypersphere, the decision boundary is expanded, and a prediction result is output. A pivot node self-supervised regularizer is introduced, center nodes are screened from labeled driver genes, positive constraints are applied to neighbor non-labeled nodes, negative penalties are applied to non-neighbors, and a supervised boundary is maintained when non-labeled data is used. Finally, a structured explanation module is used to reuse the internal evidence of the model, a verifiable explanation is provided for prediction, and the unification of high precision and credible explanation is realized.

This invention relates to the fields of synthetic biology and metabolic engineering, specifically to a Streptomyces promoter selection marker and its application. The selection marker gene is *Streptomyces lilacinus*. Streptomyces lavendulae Glutamine indigo synthase gene derived from CGMCC 4.1386 idgS This invention screens endogenous promoters of *Streptomyces freundii* using heterologously expressed idgS genes; and constructs a detection system for endogenous promoters of *Streptomyces freundii* using this scheme, which can rapidly detect endogenous promoters of *Streptomyces freundii* and the strength of each promoter.

The application discloses a method and system for rapid screening and analysis of homologous genes, and relates to the technical field of biological information and molecular breeding. The method comprises the following steps: obtaining multi-source input files; the multi-source input files comprise a reference genome file, a sample genome file, a reference annotation file and a target gene list file; pre-processing the multi-source input files to obtain pre-processed multi-source input files; the pre-processing comprises standardization processing, integrity detection, format consistency detection, repeated record detection and sequence file information improvement; performing sequence alignment, variation site extraction and variation marking on the pre-processed multi-source input files to obtain gene screening analysis results; and performing visual processing and data management on the gene screening analysis results. The application can reduce manual intervention, improve the efficiency and consistency of data analysis, and realize rapid standardized processing and intuitive result display of genomic variation information.

The invention relates to the field of animal husbandry molecular breeding and precise breeding, in particular to an animal husbandry improved variety breeding twin-fetal cattle system based on gene screening. The system comprises an ovulation potential typing module, a uterine bearing capacity quantification module, a sex compatibility verification module and a decision module. Constructing a combined evaluation model of the double-ovulation basis and the uterus metabolism capability by systematically integrating site specific amplification and gene expression quantification technologies; the core of the method is that a precise reproduction instruction is generated according to full-link data of genotypes and phenotypes; according to the method, a single ovulation rate screening mode is changed, the problem of abortion and weak abortion caused by insufficient intrauterine environment is effectively solved, and the success rate of double-fetal pregnancy is remarkably increased.

The invention relates to the technical field of gene sequencing, in particular to a pretreatment method for gene screening based on pathogenic bacteria carried by food crops, a sequencing method and application thereof. According to the pretreatment method and the sequencing method for screening the pathogenic bacteria genes of the food crops, a liquid culture medium containing a potato extracting solution and a carbon source is used for carrying out enrichment culture on a food crop sample, enrichment of the pathogenic bacteria to be detected can be well guaranteed under the nutritional condition suitable for growth of the pathogenic bacteria, especially fungi, and the detection accuracy is improved. According to the method, fungi in the sample can be effectively prevented from being broken and cracked, the concentration and quality of total DNA extraction of the subsequent sample are improved, the sequencing effect is guaranteed, and then high-throughput sequencing is achieved.

The application discloses a specific molecular marker screening method for microbial tracing, comprising the following steps: step one, microbial genome characteristic analysis; step two, specific candidate gene screening; step three, homologous arm design and target fragment amplification; step four, recombination plasmid construction; step five, positive control system establishment; step six, specificity verification; step seven, sensitivity detection; step eight, stability evaluation; step nine, marker practicability verification; and step ten, standardization and shaping; the unique gene fragment of the target microorganism is screened through whole genome alignment, and multiple verifications such as specificity, sensitivity, stability and practicability are combined, so that the screened molecular marker has high specificity, can effectively distinguish the target microorganism from the non-target microorganism close to the target microorganism, and cross reaction is avoided.

The invention provides compositions and methods for performing mammalian cell genetics, e.g., genetic screens, using near-haploid cells. The invention further provides genes and gene products isolated using the inventive methods and methods of use thereof.

The invention discloses a construction method and application of an animal model for in-vivo ubiquitination enzyme screening, and belongs to the technical field of animal model construction. The traditional in-vitro screening of ubiquitination modification enzyme genes has the defects of unreal simulation environment, poor repeatability, narrow application range and the like, 150 SgRNAs are designed to construct library plasmids, loxP TC9 sequences and filling sequences are introduced to prepare transgenic Sanyang mice, and the transgenic Sanyang mice are used for in-vivo screening after being induced by tamoxifen. The mouse model constructed by the invention can realize gene screening in 29 tissues and organs, the success rate of target verification is more than 80%, the coefficient of variation is lower than 10%, the model can be stably passed, and the screening cost and technical threshold are greatly reduced.

The present application relates to the technical field of biological detection, and a double-path apple codling moth identification system integrating RPA-LFS rapid screening and TaqMan qPCR real-time quantification, a locked nucleic acid probe primer set, a kit and a method thereof. COI Through gene screening and primer screening, the present application designs recombinase-mediated isothermal amplification (RPA) and TaqMan qPCR primers and probes for the barcode of the cytochrome oxidase subunit I (COI) gene of apple codling moth, and establishes an RPA-LFS method in combination with immunolateral flow. The RPA-LFS method can complete amplification and visual judgment within 20 minutes at 39 DEG C, and is suitable for on-site rapid detection. The TaqMan qPCR method can realize high-sensitivity quantitative analysis under the condition of crude extract. Through double-path complementary design, the consistency of the results of on-site screening and laboratory confirmation detection is realized, and the method is simple to operate and suitable for pest quarantine at ports, epidemic monitoring in agricultural production areas and quarantine at storage and transportation links.

This invention discloses a method based on SOD The primers and methods for gene screening of heat-resistant scallop varieties were developed using scallop cDNA and DNA as templates. PCR amplification was performed using upstream and downstream primers (SEQ ID No. 3 and SEQ ID No. 4, SEQ ID No. 5 and SEQ ID No. 6) to obtain samples containing scallop cDNA and DNA. SOD Gene sequence fragments from the exon and promoter regions were used as molecular markers. The genotypes were CT at the c.229 C>T site, CA at the c.284 C>A site, and CA at the c.348 C>A site in the exon region, and CA at the g.1124 A>C site and AT at the g.1359 T>A site in the promoter region. This method screened for high-temperature resistant scallop varieties, demonstrating advantages such as genetic stability, accuracy, and efficiency.

The invention provides a gene combination related to wheat flour color, a dominant gene combination and application in breeding, and belongs to the technical field of breeding. According to the invention, 12 genes influencing the color of the flour are screened by utilizing a 166 sample group, 9 dominant genes are screened, and the genes can be used as important genes of the color of the flour and have remarkable breeding utilization value. The invention proves that wheat varieties (lines) containing Psy-A1b, Pds-B1b, Lcye-A1b, Lcye-B1b, Lox-B1a, Ppo-A1b, Ppo-D1a, Pd-2D-GG / AG and non-1B / 1R translocation have advantages in flour color, the flour color is gradually improved along with the increase of the number of the wheat varieties (lines), and the wheat varieties containing the combination of at least six of the alleles have excellent flour color, can be used as excellent parents of high-brightness white flour wheat, and have broad application prospects. And an important material is provided for the cultivation of new wheat varieties.

The application relates to the field of molecular biological technology, and discloses dsRNA of a Hyphantria cunea gene and application thereof.The dsRNA of the Hyphantria cunea gene comprises at least one of LKB1 gene dsRNA, Foxo3 gene dsRNA and ECR gene dsRNA;the nucleotide sequence of the LKB1 gene dsRNA is shown as SEQ ID NO.1;the nucleotide sequence of the Foxo3 gene dsRNA is shown as SEQ ID NO.2; and the nucleotide sequence of the ECR gene dsRNA is shown as SEQ ID NO.3.Through the technical scheme, the problem of insufficient prevention and treatment of the Hyphantria cunea in the related art is solved, and the blank of screening of efficient target genes of a core channel of energy metabolism and development regulation of the Hyphantria cunea in the field of RNA interference prevention and treatment is filled.

The application discloses a construction method and application of Corynebacterium glutamicum for synthesizing shinorine, and belongs to the field of genetic engineering and fermentation technology. The Corynebacterium glutamicum ATCC 13032 is used as a starting strain, a competitive pathway gene is knocked out, an optimal exogenous enzyme is screened, and a key enzyme gene cluster is integrated in multiple copies on a genome, so that the synthesis yield of shinorine in the microbial cell is improved, and the strain can accumulate 0.63 g / L of shinorine in 72 hours in a flask. In fed-batch fermentation in a 50 L fermenter, the final yield of shinorine can reach 4.3 g / L.

This invention discloses a pleiotropic gene sharing genetic structure with obesity and mental disorders, along with its screening method and applications, belonging to the field of biomedical technology. The pleiotropic gene includes RERE, NEGR1, DENND1B, CTNNB1, TMEM106B, SP4, TSNARE1, DENND1A, CNNM2, NT5C2, BMAL1, MPHOSPH9, CCDC92, YLPM1, TAOK2, RTN4RL1, and ARFGEF2. The process includes collecting GWAS summary statistics on obesity-related phenotypes and mental disorders, assessing genetic correlation, screening pleiotropic genetic loci and candidate pleiotropic genes, using TWAS to screen pleiotropic genes, functional analysis and drug target prediction, and bidirectional causal association genetic validation. These methods are used to prepare drugs for the treatment of obesity and mental disorders or to construct a risk prediction model for obesity-mental disorder comorbidity.

The invention relates to the technical field of bioinformatics and distributed computing, and discloses a millet gene screening method and system based on data mining and a storage medium, and the method comprises the steps: obtaining millet gene data, carrying out initial clustering processing, and dividing the millet gene data into a plurality of data segments to obtain a segment set; evaluating a resource demand index of each data segment in the segment set; obtaining current load state data of the computing nodes, and distributing the data segments to the corresponding computing nodes to generate task distribution mapping; starting a gene analysis task by adopting a parallel execution mechanism, monitoring an execution progress and generating a real-time synchronous adjustment signal; carrying out migration adjustment on the task according to the adjustment signal, optimizing a task execution sequence, collecting a gene analysis result of each calculation node, and fusing key site information to obtain an integrated analysis data set; performing verification processing on the analysis data set, and outputting a final millet gene screening result; the resource utilization rate and the analysis efficiency during gene data processing are improved.

The invention provides a dairy cow ketosis regulatory gene prediction system based on multi-omics analysis and machine learning, which belongs to the technical field of molecular breeding and disease prevention and control, and comprises a data acquisition and preprocessing module, a feature set establishment module, a machine learning model establishment module and a result evaluation module, the method comprises the following steps: integrating dairy cow genome, transcriptome and metabolome data, screening candidate regulatory genes through whole genome association analysis, gene differential expression analysis, cis-eQTL positioning and co-positioning analysis, and constructing a gene expression feature set; core regulation genes are screened through L1 regularization penalty by means of a Lasso model, weights are distributed, model parameters are optimized in combination with grid search and cross validation, and model performance is evaluated through an ROC curve and an AUC value. According to the invention, an integrated technical system from gene screening to risk prediction is constructed, efficient screening of the core regulation gene and accurate prediction of ketosis risk are realized, and the economic loss of breeding is effectively reduced.

The invention belongs to the technical field of biological detection, and particularly relates to a probe-free multiple SNP (Single Nucleotide Polymorphism) typing method, a kit and application thereof. The invention provides a method for realizing multiple SNP (Single Nucleotide Polymorphism) typing without using a probe, and the method takes a melting temperature (Tm) as a discrimination index to distinguish different allele specific PCR (Polymerase Chain Reaction) products for adjusting the Tm through differential tailing so as to identify allele types of each SNP. Compared with an existing method, the reagent cost, the experiment time and the workload are greatly reduced, and meanwhile high accuracy and stability are kept. The method has the characteristics of high efficiency, expandability and economical efficiency, and is expected to play an important role in the fields of large-scale gene screening, crop breeding, clinical typing analysis and the like, so that the method has a good practical application value.

The application belongs to the field of agricultural biotechnology, and discloses a rice high-temperature-resistant gene OsACX2 and application thereof, and specifically discloses application of the OsACX2 gene in regulating the high-temperature-resistant performance of rice. The application discloses, for the first time, the application of OsACX2 in regulating the high-temperature-resistant performance of rice. Through target gene screening and CRISPR / Cas9 technology, OsACX2 is knocked out or knocked down and overexpressed, and the importance of OsACX2 in regulating the high-temperature-resistant performance of rice is proved. Meanwhile, the knockout of the gene can be used to create rice germplasm resources with high-temperature-resistant performance, and can be applied to the breeding of new rice varieties with high-temperature-resistant performance.

Described herein are systems and methods and systems for preimplantation genetic testing of an embryo derived from consanguineous parents (PGT-C). The methods involve receiving embryonic genetic data and determining a proportion of the genome in long runs of homozygosity for the entire genome (global F value) as well as for one or more regions of interest within the genome (localized F values). The localized F values are weighted based on one or more factors relating to genetic viability and genetic disorders of the embryo. The global F value and weighted localized F values may be integrated with other genetic data to predict relevant risk scores.