64 results about "Neonicotinoid" patented technology

The invention relates to the technical field of pesticide analysis and detection, in particular to a method for enriching and detecting pesticide based on garlic straw magnetic biochar. The preparation method comprises the following steps: by taking garlic straw as a carbon source, activating, calcining and carbonizing, and then mixing and reacting with a trivalent ferric salt solution and a divalent ferric salt solution to obtain the garlic straw magnetic biochar. The garlic straw magnetic biochar has a large number of pore structures, a high specific surface area, rich oxygen-containing functional groups and excellent magnetism, and has a good adsorption effect on neonicotinoid and methoxy acrylate pesticides. The garlic straw magnetic biochar is used for magnetic solid-phase extraction detection of neonicotinoid and methoxyl acrylate pesticides in an environmental water sample, and the detection recovery rate and precision are good.

The invention belongs to the technical field of gene engineering, and particularly relates to application of a drug-resistant gene CSP8 in regulation and control of resistance of diaphorina citri to neonicotinoid pesticides and a detection method of the drug-resistant gene CSP8, and the nucleotide sequence of the drug-resistant gene CSP8 is shown as SEQ ID NO.1. By regulating and controlling the drug-resistant gene CSP8, the resistance of diaphorina citri to neonicotinoid pesticides can be effectively regulated. The drug-resistant gene CSP8 disclosed by the invention can be used as a molecular target for field monitoring of diaphorina citri. Aiming at the resistance of diaphorina citri to neonicotinoid pesticides, the drug-resistant gene CSP8 is analyzed and screened based on an expression mode.

The invention discloses a nucleic acid aptamer capable of simultaneously identifying neonicotinoid pesticide imidacloprid and clothianidin and application of the nucleic acid aptamer. The nucleic acid aptamer is XQ-20 or XQ-20.1, and the nucleotide sequence of the XQ-20 is as shown in SEQ ID NO.2; the nucleotide sequence of the XQ-20.1 is as shown in SEQ ID NO. 1. The nucleic acid aptamer disclosed by the invention can be simultaneously combined with imidacloprid and clothianidin in a high affinity manner, and has the advantages of low immunogenicity, batch synthesis, good biocompatibility and stability, small molecular weight, easiness in storage and the like.

A method and system for the treatment of Monarch butterflies (Danaus plexippus Kluk (Lepidoptera: Nymphalidae) to protect them from various life-threatening conditions, including the negative effects of various pesticides, provides Monarch butterflies with the ability to assimilate and degrade pesticides such as neonicotinoids and fipronil. Certain embodiments involve the inoculation of flowers by honey bees with desired bacteria that are able to degrade pesticides, such that when Monarch butterflies visit such flowers, they are exposed to such bacteria, transforming the microbiome of the Monarch butterflies so that pesticides can be degraded, thus enhancing the health of the Monarch butterflies.

The invention discloses a rapid treatment device and method for neonicotinoid pesticide polluted water in a river network area, and belongs to the technical field of water treatment. The device comprises a mixing unit, a settling separation unit and an adsorption medium circulating unit, the mixing unit is provided with a tangential water inlet and an adsorption medium feeding port; the settling separation unit is used for realizing rapid gravity separation of the adsorbed medium and water; and the adsorption medium circulation unit is used for medium recovery and regeneration. The invention also discloses a special core-shell structure composite particle adsorption medium and a preparation method thereof, and a rapid adsorption treatment method based on the device. Through the physical process of eddy enhanced mixing-rapid settling separation, the problems that a traditional adsorption technology is low in treatment speed, prone to blockage and low in flux are solved, and the device and the method are particularly suitable for emergency treatment of sudden and large-flow trace neonicotinoid pesticide polluted water in river network areas and have the advantages of being rapid in response, large in treatment flux, reliable in operation and low in cost.

The invention belongs to the technical field of pollutant treatment, and particularly relates to a Cu < 2 + >-mediated method for degrading neonicotinoid pollutants in a water body, which comprises the following steps: S1, adjusting the initial pH value of the water body containing the neonicotinoid pollutants to 6.0-9.0; s2, adding bivalent copper ions Cu < 2 + > and hypochlorous acid HOCl into the water body, and reacting for 12-48 hours; the concentration of bivalent copper ions Cu < 2 + > is not higher than 1mg / L, and the concentration of hypochlorous acid HOCl is not higher than 10mg Cl2 / L. Compared with the prior art, the induction mechanism of Cu < 2 + > on free radical oxidation of the neonicotinoid pollutants is clarified, the degradation speed and the total degradation rate of the neonicotinoid pollutants are improved by utilizing Cu < 2 + >, the degradation rate of the neonicotinoid pollutants after 6 hours exceeds 60%, and the degradation rate of the neonicotinoid pollutants after 36 hours can reach 99%.

The present invention provides a synergistic agrochemical composition comprising at least one strobilurin, at least one neonicotinoid, and at least one pyridine-based pesticide, and a process for preparation of the composition and uses thereof.

The invention discloses an interlayer confinement structure electro-Fenton catalyst and a preparation method and application thereof.The method comprises the steps that ferric salt and polyvinylpyrrolidone are added into purified water and stirred to be dissolved, then sodium sulfide and powdered sulfur are added, after the sodium sulfide and the powdered sulfur are thoroughly dissolved, the pH of the solution is adjusted, a hydrothermal reaction is conducted, and the obtained solid matter is FeS2; gO and FeS2 are taken and subjected to ultrasonic dispersion through a solvent respectively, then mixed and subjected to self-assembly through vacuum filtration, efficient compounding of FeS and GO is achieved, the electro-Fenton catalyst of the interlayer confinement structure is obtained, and the degradation efficiency of neonicotinoid pesticide pollutants is effectively improved.

The invention provides a composite repairing agent capable of synchronously removing heavy metal cadmium and neonicotinoid pesticides and a preparation method and application of the composite repairing agent. The composite repairing agent comprises a functional fungicide and iron minerals, and the ratio of the dry weight of the iron minerals to the volume of a fungicide liquid of the functional fungicide is 1: (1-10); the functional microbial agent is a viable bacterium culture of dissimilatory iron reducing bacteria, the thallus concentration of the functional microbial agent is not lower than 1 * 10 < 8 > CFU / mL, and the iron mineral is Fe2O3.nH2O. According to the method, an iron reducing bacteria-ferrihydrite mixed system formed by in-situ active minerals is constructed, efficient treatment of combined pollution is achieved through an adsorption-conversion-degradation synergistic mechanism, and ferrihydrite is converted into magnetite in situ within 24-72 hours through iron reducing bacteria in the iron reducing bacteria-ferrihydrite; the new magnetite shows excellent dual functions, and the removal rate of Cd can be increased to gt by crystal lattices of the new magnetite through obligate adsorption and coprecipitation; meanwhile, structural ferrous iron is used as a strong reducing agent and can efficiently attack nitryl in imidacloprid molecules, and gt, gt, gt, gt, gt, gt and gt are achieved; the degradation rate is 90%.

The application discloses a comprehensive detection method and system for neonicotinoid compounds in an environment, and is characterized in that: the coverage of mass spectrum information is improved based on coupling of a multi-module data-dependent acquisition mode and a multi-window data-independent acquisition mode; a suspected screening analysis database is established based on a core structure of neonicotinoid compounds; compounds matched with the suspected screening compounds are detected; and the neonicotinoid compounds in the environment sample to be detected are subjected to non-target analysis based on coupling of a feature molecule network technology in the data-dependent acquisition mode and a feature fragment ion extraction technology in the data-independent acquisition mode, so as to back-propagate candidate compounds different from the target compounds and the suspected screening compounds; and the comprehensive detection of potential neonicotinoid compounds in the environment sample to be detected is realized through target analysis, suspected screening analysis and non-target analysis.

The application discloses a kind of ferrocarbonate / biochar composite electrofenton cathode material and its preparation method and application, the composite electrofenton cathode material (Fe3C / BC) is formed by iron-based metal organic framework material and straw mixed calcination, iron-based metal organic framework material accounts for 5% to 20% of straw mass, wherein metal iron mainly exists in the form of Fe3C.The Fe3C / BC electrofenton cathode material of the application only needs one-step hydrothermal and simple mixing grinding calcination to be prepared, the cathode material prepared can not only improve the dispersity and stability of electrofenton active site, while BC good conductivity and molecular oxygen activation capacity, can accelerate the electron transfer in system, promote the generation of active substance, thereby significantly improve the degradation effect of neonicotinoid pesticide pollutants.The electrofenton cathode material prepared by the application has excellent water matrix interference resistance and relatively wide pH and pollutant concentration application range, thus has great application value.

The present invention discloses an analytical method for detecting the residues of various pesticides in tea leaves and tea soups. The method process is as follows: firstly classify the types of pesticides to be detected, respectively draw the standard curves of the pesticide standards to be detected by gas chromatography or liquid chromatography-tandem mass spectrometry (LC-MS / MS), extract the tea leaves or tea soup samples by solvent extraction, obtain the extracted sample liquid, and obtain the pesticide residue results by the above-mentioned standard curve after testing by gas chromatography GC or LC-MS / MS. The method of the present invention has the advantages of less interference from impurities, convenient sample pretreatment, reliable precision and accuracy, etc. The GC-ECD method can be applied to the trace analysis and detection of chlorpyrifos, bifenthrin, cypermethrin and cypermethrin in tea leaves and tea soup samples; the GC-FPD method can be applied to the analysis and detection of chlorpyrifos and malathion in tea leaves and tea soup samples; the LC-MS / MS method has high sensitivity, accurate qualitative analysis, and very low detection limit, and can be widely used in the trace analysis and detection of neonicotinoid pesticides in tea leaves and tea soup samples.

The invention relates to the technical field of pesticide residue detection, in particular to a method for rapidly screening neonicotinoid and macrolide pesticide residues in vegetables through trichogramma chilonis. The 10% lethal effect condition of the trichogramma on nine kinds of common neonicotinoid or macrolide pesticides such as imidacloprid, acetamiprid, dinotefuran, thiamethoxam, clothianidin, nitenpyram, abamectin, methylamino abamectin benzoate and ivermectin is determined; acute toxic effects and action rules of nine common neonicotinoid or macrolide pesticides in vegetables on trichogramma are researched; according to the method, the 1-hour action dose of the pesticide is determined by adopting 10% of death effect, the detection limit of the trichogramma biotoxicity method on the residues of the 9 pesticides in the vegetables is calculated by combining the quality and surface area of the vegetables, and finally, the common neonicotinoid or macrolide pesticide residues in the vegetables are rapidly screened by adopting the trichogramma biotoxicity limit value determination method.

The present invention relates to the technical field of environmental and food pollution detection, and particularly relates to a method for constructing a dual-mode detection of neonicotinoid pesticide residues based on waste tobacco-based biomass carbon dots. In the present invention, waste tobacco by-products (such as leaves and stems) are used as raw materials to synthesize waste tobacco-based biomass carbon dots (CDs), which have the activities of oxidase-like (OD) and peroxidase-like (POD). On this basis, taking imidacloprid (IMI) as a neonicotinoid pesticide model, a highly efficient, low-cost, green and environmentally friendly detection of neonicotinoid pesticide residues in medicated and edible homologous samples such as wolfberry, honeysuckle, and notoginseng is established based on the dual enzyme-like activities of OD-like and POD-like of waste tobacco-based biomass carbon dots, driven by two detection modes: ultraviolet spectral absorbance and fluorescence spectral fluorescence intensity. The present invention combines the advantages of colorimetry and fluorimetry, with a dual-ratio built-in reference, effectively avoiding the false positive phenomenon caused by external signal interference, while also enhancing the sensitivity and specificity of the detection and avoiding the occurrence of false negatives.

The invention discloses a method for evaluating the ecological health risk of a typical neonicotinoid pesticide, which is characterized in that drosophila melanogaster is taken as a biological model, and the ecological health risk of the typical neonicotinoid pesticide is evaluated through comprehensive analysis of multiple indexes such as morphology (phenotype, life, development and behavior), histology, energy metabolism, oxidative stress and cell apoptosis of the drosophila melanogaster. According to the method disclosed by the invention, the influence of the typical neonicotinoid pesticide on the growth process of the drosophila melanogaster can be quickly and intuitively evaluated, and the ecological health risk of the typical neonicotinoid pesticide can be reflected; meanwhile, the method systematically evaluates the biotoxicity possibly generated when the typical neonicotinoid pesticide enters the ecological environment after being applied, makes up the research blank in the field, and plays a reference role in safe use of the typical neonicotinoid pesticide.

The invention belongs to the technical field of detection, and particularly relates to an in-situ electrochemical etching silk-screen printing silver electrode, a preparation method thereof and an EC-SERS platform. On the basis of a pesticide protonation-EC-SERS combined strategy, an independently prepared SPE-Ag electrode is used as an enhanced substrate, a mobile phone is coupled to regulate and control a portable electrochemical workstation to apply potential, an EC-SERS rapid detection platform is established, the EC-SERS rapid detection platform is successfully used for rapid and sensitive detection of the residues of the neonicotinoid pesticide imidacloprid, the detection limit is as low as 1.5 nM, and the EC-SERS rapid detection platform can be used for rapid and sensitive detection of the residues of the neonicotinoid pesticide imidacloprid. According to the present invention, the problem that the on-site real-time application of the EC-SERS technology is limited by the equipment size, the cost and the operation complexity is solved, the portable and low-threshold on-site detection platform is provided, the detection cost is significantly reduced while the sensitivity of the traditional EC-SERS is maintained by the platform, the operation threshold is substantially reduced, and the detection efficiency is improved. And a method with a better application prospect is hopefully provided for on-site real-time detection of pesticide residues.

The invention belongs to the technical field of pesticide control, and relates to a pesticide composition and application. The pesticide composition comprises an active component A and an active component B, the active component A is a compound represented by a formula (I), the active component B is a neonicotinoid compound, and the mass ratio of the active component A to the active component B is 1: 85-45: 1. The pesticide composition disclosed by the invention has the advantages of obvious synergistic effect, fast pesticide effect, long lasting period, increased acting sites, expanded prevention and control spectrum and capability of reducing the field dosage of effective components, so that the production and use components are reduced, and the generation of drug resistance of pests and pest mites is delayed.

The invention relates to the technical field of genetic engineering and pesticide detection, in particular to a recombinant plasmid, a genetically engineered bacterium and a method for detecting neonicotinoid pesticides in an environment. According to the invention, a genetic engineering bacterium is constructed by cloning a ligand binding domain ECD part of a nicotine acetylcholine receptor alpha7 subunit into an expression vector and further expressing recombinant plasmids by using escherichia coli. According to the invention, the genetically engineered bacterium is used as a single fluorescent protein bioreceptor for detecting neonicotinoid pesticides in an environment, and the detection method does not need expensive instruments and professional detection personnel, and does not need tedious pretreatment and long-time culture incubation or analysis; according to the method, the environmental neonicotinoid pesticide can be subjected to targeted in-situ rapid identification and quantitative detection, so that the detection time is shortened, the development of an environmental neonicotinoid pesticide analysis and detection method is promoted, and the method is of great significance to subsequent environmental neonicotinoid pesticide control strategy development and water environment ecological protection.

The invention belongs to the field of chemical ecology of agricultural pests and pesticide science, and relates to an insecticide composition for improving the sensitivity of agricultural pests to insecticides and application of the insecticide composition. Folic acid is applied to a pest feeding substrate, a host plant or a feed, so that the sensitivity of pests to various insecticides (including neonicotinoids, pyrimidines, pyrethroids, organophosphorus and the like) can be remarkably enhanced. It is found that folic acid can regulate and control a carbon metabolism network in pests and inhibit cytochrome P450 and other detoxification enzyme related pathways, and then the endurance capacity of the pests to insecticides is reduced. The composition and the method are verified in seven important pests such as brown planthopper, sogatella furcifera, spodoptera frugiperda, cotton aphid, chilo suppressalis, panonychus citri and thrips, have the advantages of improving the pesticide effect, reducing the pesticide application amount and delaying the resistance evolution, and can be widely applied to comprehensive treatment of agricultural pests.

The invention discloses a preparation method of a modified nano composite material and application of the modified nano composite material in degradation of new pollutants, and belongs to the field of new pollutant treatment. According to the preparation method disclosed by the invention, LaFeO3 is doped into MIL-88A to synthesize heterojunction MIL-88A-LaFeO3, and citric acid is added, so that the photocatalytic performance of the material is improved. The photo-Fenton degradation efficiency of the MIL-88A-coated LaFeO3 prepared by the preparation method disclosed by the invention on acetamiprid reaches 99.9% within 180 minutes, and the photo-Fenton degradation efficiency of the MIL-88A-coated LaFeO3 prepared by the preparation method disclosed by the invention on sulfamethoxazole reaches 100% within 180 minutes. The photo-Fenton degradation efficiency of the composite material modified by citric acid on acetamiprid reaches 100% within 75 min, and the composite material shows more excellent photocatalytic activity. The preparation method disclosed by the invention is simple in material and low in cost, and the prepared material is high in degradation rate, has broad spectrum and is applied to degradation of various types of neonicotinoid pesticides and antibiotics in a water body.

The invention belongs to the field of pesticide pollution remediation. The invention provides a preparation method of a TiO2-ZnO photocatalyst for efficiently degrading neonicotinoid pesticides, which comprises the following steps: (1) grinding and fully mixing ZnO and 2-methylimidazole, transferring into a reaction kettle, and reacting to obtain an MOFs material ZIF-8; (2) adding polyethyleneimine and acetic acid into tetrabutyl titanate, stirring, then adding ZIF-8, and continuously stirring; (3) transferring the mixed solution in the step (2) into a polytetrafluoroethylene reaction kettle for reaction; (4) washing and drying the precursor substance after the reaction in the step (3); and (5) transferring the precursor substance obtained in the step (4) into a crucible, and carrying out high-temperature pyrolysis in a tubular furnace in an air atmosphere to obtain TiO2-ZnO. The invention further provides the TiO2-ZnO photocatalyst for efficiently degrading the neonicotinoid pesticide and application of the TiO2-ZnO photocatalyst. The TiO2-ZnO photocatalyst provided by the invention shows good degradation performance and long-term stability on the neonicotinoid pesticide. The synthesis steps are simple and suitable for industrial large-scale production.

The invention discloses a shiitake mushroom dreg polysaccharide nano-selenium fertilizer and a preparation method and application thereof, the shiitake mushroom dreg polysaccharide nano-selenium fertilizer comprises shiitake mushroom dreg polysaccharide and nano-selenium, and the shiitake mushroom dreg polysaccharide coats the surface of the nano-selenium; the preparation method comprises the following steps: preparing crude polysaccharide from shiitake mushroom residues by adopting ultrasonic-assisted extraction, hydrothermal extraction, enzymolysis deproteinization and alcohol precipitation processes; taking the polysaccharide as a carrier, and reacting with sodium selenite and ascorbic acid to prepare the flower mushroom dreg polysaccharide nano-selenium fertilizer. The method realizes resource utilization of the mushroom dregs, and is low in cost and simple and efficient in process. The obtained fertilizer is good in stability and high in biological activity, can remarkably increase the content of selenium and microelements in vegetables, reduce pesticide residues, relieve neonicotinoid pesticide stress and salt damage of saline-alkali soil, enhance the osmotic regulation capacity of crops and improve biomass when applied to crops, and provides a new path for efficient agricultural production of the saline-alkali soil.

The invention relates to a plasma activated water enhancement-based pesticide composition and application thereof, and aims to solve the technical problems of drug resistance crisis, environmental residue pollution and unstable control effect existing in chemical control of cotton aphids. Plasma activated water (PAW) and neonicotinoid / picolinamide insecticides are compounded to form a synergistic system with the concentration of LC10-LC75, oriented application is conducted on the basis of the insect age adaptation rule (imidacloprid is used for evaluation for 24 h, flonicamid is used for evaluation for young aphids, 72 h is used for evaluation for young aphids), the epidermis structure of cotton aphids is destroyed through reactive oxygen nitrogen substances (RONS) in the PAW, the permeability of the pesticides is enhanced, and therefore the cotton aphids with the concentration of LC10-LC75 are obtained. The comprehensive technical effects of remarkably improving the fatality rate of the cotton aphids at each age, reducing the pesticide dosage, reducing environmental residues and delaying the development of drug resistance are achieved.

The present application relates to PxGSTD2 protein mutants, and the use of wild type and mutants of PxGSTD2 protein in catalyzing the conjugated coupling of pesticides with reduced glutathione (GSH) and improving the pesticide tolerance of non-target organisms. The PxGSTD2 protein and its mutants provided by the present application catalyze the conjugated coupling of various types of pesticides such as oxadiazines, neonicotinoids, pyrethroids and the like with GSH, improve the water solubility of the pesticides, reduce the toxicity of the pesticides, form adducts that are easily excreted out of the body by organisms, and thus significantly enhance the tolerance of non-target organisms to pesticides.

The invention belongs to the technical field of environment and food pollution detection, and particularly relates to a method for detecting neonicotinoid pesticide residues by constructing a dual mode based on waste tobacco-based biomass carbon dots. According to the method, waste tobacco byproducts (such as leaves and stems) are used as raw materials to synthesize the waste tobacco-based biomass carbon dots (CDs), and the carbon dots have oxidase-like (OD) and peroxidase-like (POD) activities. On the basis, imidacloprid (IMI) is used as a neonicotinoid pesticide model; two detection modes of ultraviolet spectrum absorbance and fluorescence spectrum fluorescence intensity driven by OD-like and POD-like enzyme activity based on the waste tobacco-based biomass carbon dots are established for efficient, low-cost and environment-friendly detection of neonicotinoid pesticide residues in medicinal and edible samples such as Chinese wolfberry, honeysuckle and pseudo-ginseng. The advantages of a colorimetric method and a fluorescence method are integrated, double-ratio built-in reference is adopted, the false positive phenomenon caused by external signal interference is effectively avoided, meanwhile, the detection sensitivity and specificity are enhanced, and false negative is avoided.

The invention provides a silica gel foam sealing material capable of preventing termite gnawing and a preparation method of the silica gel foam sealing material, and belongs to the technical field of polymer sealing materials. The raw material formula of the silica gel foam sealing material is as follows: vinyl silicone oil, a reinforcing filler, a flame-retardant filler, a contact insecticide, a bait, an odorant, a structure control agent, an inhibitor, a platinum catalyst, a foaming agent, hydroxyl silicone oil and hydrogen-containing silicone oil. Wherein the contact insecticide is one or more of pyrethroids, neonicotinoids, phenyl pyrazoles, boric acid, glycine, erythritol and fipronil; the bait agent is one or more of diatomite, talcum powder, capsaicin, dihydrocapsaicin and denatonium benzoate; the odorant is one or more of camphor oil, peppermint oil, limonin, limonene, citral, capraldehyde, octanal and nonanal. The silica gel foam sealing material disclosed by the invention has excellent flame retardant property, mechanical property and ant gnawing resistance, and the integrity of the sealing material can be well protected.

The present application belongs to the field of pesticide insecticide, and relates to a neonicotinoid-containing pesticide composition and use. The pesticide composition comprises active ingredient A and active ingredient B, the active ingredient A is a compound of formula (I), the active ingredient B is a neonicotinoid compound, and the mass ratio of the active ingredient A to the active ingredient B is 1:50-40:1. The pesticide composition of the present application has the advantages of synergism and resistance delay, and is used for preventing and treating resistant pests.

The present invention belongs to the field of pesticide control technology and relates to a pesticide composition and its application. The pesticide composition comprises active ingredient A and active ingredient B, wherein active ingredient A is a compound represented by formula (I) and active ingredient B is a neonicotinoid compound, with the mass ratio of active ingredient A to active ingredient B being 1:85 to 45:1. The pesticide composition of the present invention exhibits significant synergistic effects, rapid efficacy, a long-lasting effect, increased sites of action, a broadened control spectrum, and can reduce the field dosage of the active ingredient, thereby reducing production and usage, and helping to delay the development of pesticide resistance in pests and mites.

The application discloses a neonicotinoid pesticide-heavy metal compound contaminated soil remediation method, which comprises the following steps: after ploughing and leveling the neonicotinoid pesticide-heavy metal compound contaminated soil, applying bottom fertilizer and neonicotinoid pesticide remediation agent in sequence, ploughing and maintaining, and then transplanting grain amaranth seedlings; when the grain amaranth seedlings grow to the present budding stage, spraying a heavy metal activator to adsorb the soil heavy metal. The application can effectively adsorb, extract and degrade the heavy metal and the neonicotinoid pesticide in the neonicotinoid pesticide-heavy metal compound contaminated soil, reduce the heavy metal and the pesticide residue in the soil, and has the advantages of fast remediation speed, good effect, simple operation and large treatment capacity.