38 results about "Malathion" patented technology

The invention relates to a pesticide residue detection test strip as well as a preparation method and application thereof, in particular to a rapid detection technology based on an enzyme inhibition method and a chitosan non-woven fabric carrier. The test strip can specifically detect organophosphorus and carbamate pesticides such as trichlorfon, malathion and phoxim through the enzyme activity inhibition principle, the detection limit of the pesticides such as phoxim is as low as 0.1 mg / kg, the developing intensity is in negative correlation with the pesticide concentration, and semi-quantitative judgment can be achieved. The preparation process is simple, the chitosan non-woven fabric is adopted as a fixed carrier, the material is environment-friendly and degradable, the enzyme activity recovery rate reaches 90%, the stability is excellent (the enzyme activity recovery rate can be preserved for 60 days at the temperature of 4 DEG C in a dark place), sample pretreatment is not needed in detection operation, a result can be obtained at the normal temperature for 15 min, the stability and repeatability are good, and the problems that a traditional instrument is high in detection cost and long in time consumption are solved; the method has a remarkable basic popularization value.

The invention belongs to the technical field of environmental engineering, and discloses a microbial agent for degrading malathion as well as a preparation method and application of the microbial agent. The microbial agent comprises the following raw materials: compound bacteria, a nutritional supplement and a biomass carrier material, wherein the compound bacteria comprise functional bacteria and synergistic bacteria, and the mass ratio of the functional bacteria to the synergistic bacteria is (1.2-1.8): 1; the functional bacteria comprise issatchenkia orientalis, nocardia, brevibacillus laterosporus and bacillus cereus; the synergistic bacteria comprise azospirillum brasilense, bacillus thuringiensis and lactobacillus buchneri. Functional bacteria with direct degradation capacity and synergistic bacteria with auxiliary functions are creatively compounded according to a specific proportion, meanwhile, the garlic straw powder is selected as a biomass carrier material, all the raw materials complement each other in function and have a synergistic effect, the degradation rate and thoroughness of malathion are remarkably improved, and the degradation efficiency of malathion is greatly improved. The defects of low degradation efficiency and poor environmental adaptability of a single strain are overcome.

The invention belongs to the field of detection, and particularly relates to an electrochemical sensing platform for detecting lead ions and malathion as well as a preparation method and a detection method of the electrochemical sensing platform. The preparation method of the electrochemical sensing platform comprises the steps of designing a three-chain DNA compound, preparing a gold nanoparticle electrode, preparing the electrochemical sensing platform and the like. The electrochemical sensing platform realizes dual detection of lead ions (in a signal enhancement mode) and malathion (in a signal weakening mode) through specific recognition of DNAzyme and an aptamer on the basis of a three-chain DNA compound and a gold nanoparticle electrode. The method has the advantages of no label, high sensitivity (the detection limits respectively reach 5.42 pM and 0.34 pM), strong anti-interference capability and good stability, and is suitable for rapid detection of trace pollutants in environment and food samples.

The invention belongs to the technical field of sensor materials and malathion detection, and relates to a nano-silver modified covalent organic framework composite material, a preparation method and application thereof, and an electrochemical aptamer sensor. The preparation method comprises the following steps: S1, dissolving 1, 3, 5-tri (4-aminophenyl) benzene and 2, 5-divinyl-1, 4-phthalaldehyde in acetonitrile, adding a catalyst to carry out condensation reaction, standing, separating, washing and drying to obtain a covalent organic framework material; s2, dispersing the covalent organic framework material and silver nitrate in water, adding sodium citrate after ultrasonic dispersion, and washing and drying to obtain the nano-silver modified covalent organic framework composite material. The nano-silver modified covalent organic framework composite material prepared by the invention shows excellent crystallinity, extremely high porosity and good conductivity, and realizes high-sensitivity, rapid and accurate quantitative analysis of malathion in traditional Chinese medicinal materials.

The invention relates to the technical field of organic detection, in particular to a nanoparticle modified quantum dot MOF material and a preparation method and application thereof. A zirconium-based double-ligand metal organic framework material is used as a carrier, a co-reaction accelerant Ti3C2 quantum dot is modified to enhance the electrochemical luminescence reaction efficiency, and copper-gold nanoparticles are loaded to amplify signals. The nanoparticle modified quantum dot MOF material provided by the invention can be prepared into an aptamer biosensor for accurately identifying malathion, and has high sensitivity and high selectivity. According to the invention, a nanoparticle modified quantum dot MOF material is coupled and combined with an aptamer, and an aptamer probe with high electrochemical luminescence activity and specific sensitivity to malathion is constructed. When the malathion acts on the aptamer probe, the malathion is combined with the aptamer in a high affinity manner, the aptamer probe is separated from the surface of the electrode, and meanwhile, an electrochemical luminescence signal is changed, so that the malathion residue in the agricultural product is rapidly detected.

The invention relates to the field of chemical warfare agent simulation agent synthesis, in particular to a method for synthesizing malathion in a laboratory. The method comprises the following steps: firstly, reacting methanol with phosphorus pentasulfide in a toluene medium to generate dimethoxy dithiophosphate, then reacting maleic anhydride with ethanol in a benzene and sulfuric acid medium to generate diethyl maleate, and finally synthesizing the malathion from the dimethoxy dithiophosphate and the diethyl maleate. According to the method, common instruments / equipment in a laboratory are used as a synthesis device, and phosphorus pentasulfide, methanol and ethanol are used as raw materials to prepare a malathion finished product with high yield, high purity and high safety; according to the method disclosed by the invention, 50 mL of malathion of which the purity is greater than or equal to 90% can be prepared at a time by controlling the technological process of each link and setting and adjusting parameters, so that the problem of safely synthesizing a small amount of malathion of which the purity is higher in a laboratory is solved.

The invention discloses an extraction head for detecting malathion residues in agricultural products and a detection method applying the extraction head, the extraction head is a composite material of poly (5-amino indole pyrrole)-ionic liquid-porous nitrogen-doped graphene, and the ionic liquid is 1-butyl-3-methylimidazolium hexafluorophosphate. The extraction head has the beneficial effects that the extraction head made of the composite material has relatively good stability and repeatability, and can be used for rapidly detecting the residue of malafos sulfur in agricultural products.

This invention relates to the field of food safety testing technology and discloses an automatic matching method for batch parameters of malathion test cards with QR code traceability. The method includes collecting the dual-matrix Lab color space values ​​of the QR code label paper and NC film in the test card, constructing an aging decay function relationship table and an initial coupling correlation matrix, and encoding an encrypted QR code. During testing, a single frame of original image is acquired to extract real-time chromaticity feature vectors of the dual-matrix region, and the aging feature modulus is calculated by combining it with the initial coupling correlation matrix. Based on the aging feature modulus, the aging decay function relationship table is queried to dynamically correct the initial standard curve parameters and generate a real-time standard curve, thereby outputting the malathion concentration. This invention constructs an intrinsic aging indication mechanism through dual-matrix chromaticity difference, achieving adaptive reconstruction of the standard curve according to the aging state of the test card, ensuring detection accuracy throughout the entire lifecycle.

The present invention discloses an analytical method for detecting the residues of various pesticides in tea leaves and tea soups. The method process is as follows: firstly classify the types of pesticides to be detected, respectively draw the standard curves of the pesticide standards to be detected by gas chromatography or liquid chromatography-tandem mass spectrometry (LC-MS / MS), extract the tea leaves or tea soup samples by solvent extraction, obtain the extracted sample liquid, and obtain the pesticide residue results by the above-mentioned standard curve after testing by gas chromatography GC or LC-MS / MS. The method of the present invention has the advantages of less interference from impurities, convenient sample pretreatment, reliable precision and accuracy, etc. The GC-ECD method can be applied to the trace analysis and detection of chlorpyrifos, bifenthrin, cypermethrin and cypermethrin in tea leaves and tea soup samples; the GC-FPD method can be applied to the analysis and detection of chlorpyrifos and malathion in tea leaves and tea soup samples; the LC-MS / MS method has high sensitivity, accurate qualitative analysis, and very low detection limit, and can be widely used in the trace analysis and detection of neonicotinoid pesticides in tea leaves and tea soup samples.

The invention relates to the technical field of preparation of functional water-based asphalt coatings, in particular to a water-based asphalt anti-insect and anti-corrosion coating and a processing technology thereof. The invention relates to a water-based asphalt anti-insect and anti-corrosion coating which consists of a water-based asphalt basic coating and water-based anti-insect polymer latex, the water-based insect pest prevention polymer latex comprises deionized water, an emulsifier, polymer latex particles and insect pest prevention filler; the insect pest prevention filler comprises a porous inorganic filler carrier and an insect pest prevention agent loaded on the porous inorganic filler carrier, and the porous inorganic filler is one of zeolite, halloysite and porous silicon dioxide; the insect pest prevention agent is at least one of 4-nitrophenyl carbamate, (3-methyl azetidin-3-yl) methyl tert-butyl carbamate, malathion monocarboxylic acid, a folium artemisiae argyi extract, an azadirachta indica extract, a fresh ginger extract, a cinnamomum camphora leaf extract and a fresh ginger extract. The anti-corrosion and anti-rust paint can play an effective anti-corrosion and anti-rust effect and also has an excellent and lasting effect of preventing plant diseases and insect pests.

The invention belongs to the technical field of microorganisms, and particularly relates to a composition for synchronously removing organophosphorus pesticide and heavy metal combined pollution, a preparation method and a removal method. The invention further provides an iron-carbon material and shewanella composite system, the composite system can enable thalli to be attached to the surface of the FeO / C material and grow into a biological membrane, and synchronous removal of composite pollution by the composite system is promoted; meanwhile, electron transfer is improved, so that heavy metal reduction and organophosphorus pesticide degradation are accelerated. Through verification, the compound system also effectively relieves the toxic effect of malathion and Cr (VI) compound pollution on cells. By applying the composite system disclosed by the invention, the composite pollution of malathion and Cr (VI) can be synchronously removed, and the removal rate of malathion and Cr (VI) is increased.

The invention discloses a method for multicolor detection of malathion based on Fe-Zr bimetallic nano-enzyme, a Fe-Zr bimetallic active center is formed, the synthesized Fe3O4-coated PDA-coated UiO-66 shows excellent peroxidase-like catalytic activity, the malathion can be combined with a composite material through a Zr-O-P bond, and the composite material can be used for detecting the peroxidase-like catalytic activity of the malathion, so that the malathion-coated UiO-66 can be used for detecting the peroxidase-like catalytic activity of the malathion-coated UiO-66, and the malathion-coated UiO-66 can be used for detecting the peroxidase-like catalytic activity of the malathion. By combining the Au NRs, multicolor detection of malathion is realized. The multicolor colorimetric detection method disclosed by the invention gets rid of the limitation that the traditional colorimetric method depends on different shade changes of a single color, so that the visual resolution is relatively poor, and the colorimetric signal can be combined with the smart phone, so that on-site instant detection is facilitated; meanwhile, a new way is provided for designing and applying the effective nano-enzyme to carry out pesticide field monitoring.

The invention relates to a method for detecting pesticide based on a ferriporphyrin modified covalent organic framework nano-enzyme sensing array. According to the invention, ferriporphyrin molecules are loaded on a porous and ordered COFs skeleton, so that excellent peroxidase simulated catalytic activity is realized, H2O2 can be catalyzed to be decomposed to generate reactive oxygen free radicals, and TMB, OPD and ABTS chromogenic reactions are catalyzed. Based on the performance, a three-channel colorimetric sensing array is constructed to detect and distinguish various pesticides such as malathion, profenofos, chlorpyrifos, acephate and dimethoate, and the detection limit is as low as 0.341 nM. After different pesticides are adsorbed to active sites, catalytic reaction can be remarkably inhibited, and specific colorimetric fingerprint signals are generated. Colorimetric data is further combined with a machine learning algorithm, high-accuracy classification and recognition of single pesticides and mixtures thereof are achieved by means of a multilayer sensor, a support vector machine, a random forest and other models, the classification accuracy of an MLP model reaches 90%, ROC curve AUC exceeds 0.97, and the reliability of the detection method is proved.

The invention belongs to the technical field of feed processing, and discloses a compound bacterium enzyme additive for improving the quality of corn green and yellow silage feed and application, the additive comprises a core functional group and an optional functional group; the core functional group consists of xylanase and lactobacillus plantarum, the activity of the xylanase is 100000Ug, and the addition amount of the xylanase is 0.25 g / kg; the activity of the lactobacillus plantarum is 1 * 10 CFUg, and the addition amount is 1mL / kg; the optional functional group is cellulase, the activity of the cellulase is 20000Ug, the addition amount of the cellulase is 0.1 g / kg, and the optional functional group is used for reducing malathion residues in the silage corn. The green / yellow silage treated by the method has the fragrance of aromatic wine, the stem and leaf structure is well kept, the color is consistent with that of raw materials, and the sensory score reaches 20 (superior grade) which is far superior to the medium grade of a control group.

The present invention discloses a molecular marker for malathion resistance of the oriental fruit fly and its application in malathion content detection. The molecular marker is the oriental fruit fly odor-binding protein BdorOBP28a-2 gene. Also disclosed are a primer pair, a kit, and a detection method for detecting the molecular marker: using an oriental fruit fly pesticide-sensitive population as a control, detecting the expression level of the OBP28a-2 gene of the oriental fruit fly to be tested; if the expression level of the OBP28a-2 gene of the oriental fruit fly to be tested is significantly higher than that of the sensitive population, it can be determined that the oriental fruit fly to be tested is a malathion-resistant population; if there is no significant difference in the expression level of the OBP28a-2 gene between the oriental fruit fly to be tested and the sensitive population, it cannot be determined that the oriental fruit fly to be tested is a malathion-resistant population. The present invention identifies and verifies for the first time that the oriental fruit fly odor-binding protein gene OBP28a-2 regulates the oriental fruit fly's resistance to malathion.

The invention discloses a preparation method and application of a pH-responsive multi-enzyme active fluorescent nano-enzyme, and belongs to the technical field of nano-enzyme preparation and application research. The multi-enzyme active fluorescent nano-enzyme is prepared by taking D-tryptophan or L-tryptophan, silver nitrate and chloroauric acid as raw materials and adopting a heating reflux method, and the preparation process is simple and convenient to operate, green, mild, stable and efficient. The prepared multi-enzyme active fluorescent nano-enzyme not only has strong blue fluorescence, but also shows excellent multi-enzyme activity, including oxidase, laccase and catecholase, and switching of the three enzyme activities can be realized by adjusting pH. In addition, the method is applied to quantitative detection of malathion and 2, 4-dichlorophenol by utilizing the characteristics of different enzyme activities.

The invention discloses an SERS detection method for organophosphorus compounds DMMP, DECMP and malathion, and relates to the field of surface enhanced Raman spectrum detection. The method comprises the following specific steps: preparing a metal 1 monatomic precursor solution; dropwise adding a metal 2 compound into the metal 1 monatomic precursor solution, uniformly stirring, diluting, adjusting the pH value, and putting a beaker containing the mixed solution into UV photocuring equipment for illumination, so as to synthesize metal 2 nano-particle sol; centrifugally cleaning with an acid solvent or an organic solvent, and concentrating; immersing a silicon wafer into the nanoparticle sol, continuously illuminating for several minutes, standing for a period of time, illuminating again, and repeating the steps to prepare the SERS substrate; a to-be-detected object is taken and dropwise added to the SERS substrate, and a Raman spectrometer is used for detection. The hot spot density and the hot spot utilization rate of the multilayer SERS substrate are improved, and SERS quantitative detection of dimethyl methylphosphate, diethyl chloromethylphosphate and malathion is realized.

The present invention relates to an improved synthesis of malathion. The presence of an acid facilitates the reaction between O,O-dimethyldithiophosphoric acid (O,O-DMDTPA) and maleate, leading to excellent product yield in a shorter reaction time and with fewer impurities.

The invention belongs to the field of environment detection, and particularly relates to application of bimetal Pt / Co nano-enzyme to instant portable detection of malathion. The bimetallic Pt / Co nano-enzyme for malathion detection is ZIF-67NF loaded with Pt nanoparticles and has peroxidase activity, nucleotide can enhance the peroxidase activity of the bimetallic Pt / Co nano-enzyme under an alkaline condition, a colorimetric reaction is started by combining the inhibition effect of malathion on alkaline phosphatase and the decomposition effect of the alkaline phosphatase on the nucleotide, and the sensitivity of malathion detection is improved. The method is used for detecting malathion, has the advantages of low detection cost, instantaneity, accuracy and the like, and provides a new method for detecting malathion.

The invention belongs to the technical field of pollutant detection, and relates to a Fe-MOF / Fe3S4 composite material and an application of the Fe-MOF / Fe3S4 composite material as a nano enzyme in malathion detection. The preparation method comprises the following steps: mixing a ferric trichloride hexahydrate solution and a fumaric acid solution to obtain a mixed solution A, and then carrying out a reflux reaction to obtain an Fe-MOF precursor; and adding a sulfur source into an ethanol dispersion liquid of the Fe-MOF precursor, carrying out ultrasonic treatment, sealing, and carrying out a hydrothermal reaction to prepare the Fe-MOF / Fe3S4 composite nanomaterial. The preparation method provided by the invention is simple and convenient in process, low in raw material price, rich in resource, low in synthesis cost, stable in product performance and suitable for large-scale synthesis. Visual detection of malathion can be realized through color change of the solution, and the method has the advantages of high sensitivity, low detection limit, wide linear response range and good selectivity and stability.

The invention discloses a method for preparing malathion by adopting a solid acid catalyst, and belongs to the technical field of organophosphorus pesticide synthesis. O, O-dimethyl dithiophosphoric acid and diethyl maleate are used as raw materials, solid acid is used as a catalyst, and addition reaction is performed to prepare malathion; the solid acid is selected from one or more of ethylenediaminetetraacetic acid, sulfonated carbon, sulfate zirconium oxide and silicon-aluminum oxide. The process provided by the invention breaks through the technical bottleneck of catalyst selection in malathion synthesis, solves the environmental protection pain point and the efficiency bottleneck in the prior art through innovative application of specific solid acid, and has the advantages of recoverable catalyst, high raw material conversion rate, excellent product purity, simple process, low energy consumption and the like. The method does not need to greatly transform the existing industrial production device, can be directly popularized and applied, can effectively reduce the production cost, reduce the environmental pollution and improve the product competitiveness, is suitable for large-scale production of agricultural, hygiene and epidemic prevention and medicinal malathion, and has wide industrial application prospects and remarkable economic, social and environmental benefits.

The invention discloses a dual-mode electrochemical / colorimetric aptamer sensor for detecting malathion in vegetables. The sensor is combined with magnetic Fe3O4 (at) ZIF-8-DNA (deoxyribonucleic acid) and CuZr-MOF-cDNA (complementary deoxyribonucleic acid), and a target object can be detected through electrochemical reaction and colorimetric change. The CuZr-MOF not only improves the electrochemical detection sensitivity, but also can catalyze the chromogenic reaction to realize colorimetric detection. The magnetic Fe3O4 ZIF-8 realizes solid-liquid separation under the action of an external magnetic field, and the sensor has a wider detection range due to the large specific surface area of the magnetic Fe3O4 ZIF-8. When the target malathion exists, the aptamer is combined with the target malathion, CuZr-MOF-cDNA is released, colorimetric signals with different intensities are generated, and meanwhile, the target in the precipitate can be quantitatively detected through an electrochemical method. Experimental results show that a colorimetric signal and an electrochemical signal have a good linear relationship. The sensor is high in sensitivity, good in selectivity and suitable for on-site rapid detection.

The application discloses a time sequence control type composite pollution repair material for facility agriculture soil and a preparation method and application thereof, and belongs to the technical field of soil repair. The repair material is a three-layer core-shell structure particle, which comprises, from inside to outside, an inner core layer composed of biodegradable polyester wrapping pesticide degrading enzyme and pesticide adsorbent, a middle layer composed of water / ion responsive hydrogel wrapping heavy metal fixing agent and oxidative degradation catalyst, and an outer shell layer composed of brittle degradable polymer wrapping micro / nano plastic surface capping agent. The repair material realizes time sequence and synergistic repair of micro / nano plastic, available Cd and malathion by triggering the release of the outer shell layer through plowing mechanical force, triggering the slow release of the middle layer through irrigation water response, and triggering the release of the inner core layer through rhizosphere microbial enzyme. The material has simple preparation process, convenient application and good application prospect.

This invention provides the application of Fe / C composite material combined with *S. wanandii* in the degradation of the pesticide malathion, belonging to the field of pesticide degradation technology. This invention provides the application of *S. wanandii* in the degradation of the pesticide malathion, offering a new method for the biodegradation of malathion. This invention also provides a material for degrading the pesticide malathion, comprising an Fe / C composite material and *S. wanandii* loaded within the pores of the Fe / C composite material. The porous structure of the Fe / C composite material serves as a habitat for *S. wanandii*. Scanning electron microscopy reveals clusters of deposits on the spiral vessels of the Fe / C composite material, within the vascular bundles, and within the pits on the radial and transverse walls of the fibrous cell cavities. *S. wanandii* colonizes and grows within these pores, and the Fe / C composite material enhances the degradation of malathion by *S. wanandii*.

This invention belongs to the field of food safety testing technology, and relates to a porous zirconium-based nanozyme and its application in the detection of malathion. Tetra(4-carboxyphenyl)porphyrin and zirconium oxychloride octahydrate are added to N,N-dimethylformamide and mixed thoroughly; then, a hydrothermal reaction is carried out with glacial acetic acid solution to obtain PCN-224 spheres. These spheres are then added to an ethanol solution and mixed thoroughly, followed by the addition of chloroplatinic acid solution and sodium borohydride solution, and then stirred to obtain PCN-224@Pt. The material synthesized by this invention has a mesoporous structure of porous nanospheres, the raw materials are inexpensive, the performance is stable, and it is suitable for large-scale synthesis. It enables the visual detection of malathion.

The application provides a beta-cyclodextrin modified electrochemiluminescence gold cerium bimetallic nanocluster, a preparation method and application thereof in electrochemiluminescence-impedance dual-mode detection of malathion, and relates to the field of malathion detection. The preparation method of the beta-cyclodextrin modified electrochemiluminescence gold cerium bimetallic nanocluster comprises the following steps: after gold source and cerium source are incubated with methionine for the first time in an alkaline environment, the gold source and the cerium source are dispersed in an ammonia solution to be incubated for the second time; and finally, the gold source and the cerium source are incubated with NH2-beta-cyclodextrin for the third time to obtain the beta-cyclodextrin modified electrochemiluminescence gold cerium bimetallic nanocluster. The beta-cyclodextrin modified electrochemiluminescence gold cerium bimetallic nanocluster is used as an ECL luminophore, and a sensor using triethylamine as a co-reaction agent is applied to the detection of malathion, and the sensor has high sensitivity, selectivity, stability, realizes ECL and EIS dual-mode cooperative detection and mutual verification, and has the advantages of simple operation and good repeatability.

The application provides a beta-cyclodextrin modified electrochemiluminescence gold cerium bimetallic nanocluster, a preparation method and application thereof in electrochemiluminescence-impedance dual-mode detection of malathion, and relates to the field of malathion detection. The preparation method of the beta-cyclodextrin modified electrochemiluminescence gold cerium bimetallic nanocluster comprises the following steps: after gold source and cerium source are incubated with methionine for the first time in an alkaline environment, the gold source and the cerium source are dispersed in an ammonia solution to be incubated for the second time; and finally, the gold source and the cerium source are incubated with NH2-beta-cyclodextrin for the third time to obtain the beta-cyclodextrin modified electrochemiluminescence gold cerium bimetallic nanocluster. The beta-cyclodextrin modified electrochemiluminescence gold cerium bimetallic nanocluster is used as an ECL luminophore, and a sensor using triethylamine as a co-reaction agent is applied to the detection of malathion, and the sensor has high sensitivity, selectivity, stability, realizes ECL and EIS dual-mode cooperative detection and mutual verification, and has the advantages of simple operation and good repeatability.

The invention belongs to the technical field of food safety detection, and particularly relates to an electrochemical luminescence sensor and application of the electrochemical luminescence sensor in multiple detection of malathion and phorate. In order to detect a target object with high sensitivity and high selectivity, the preparation method comprises the following steps: firstly, synthesizing Co-PTC MOF by taking perylene tetracarboxylic acid potassium as a ligand, and preparing an Ag-Co-PTC composite material on the surface of the Co-PTC MOF through photo-reduction of AgNO3; the electrochemical luminescence biosensor based on the Ag-Co-PTC composite material is constructed by taking Ag-Co-PTC as an electrochemical luminescence signal probe and combining CRISPR / Cas12a and a spherical nucleic acid conversion technology, so that the dual detection of malathion and phorate is realized, and the detection limits are 1.08 * 10 <-13 > M and 1.01 * 10 <-12 > M.

The invention provides a pretreatment method and a detection method of a malathion biomarker in blood, and relates to the technical field of analysis and detection. The method comprises the following steps: separating dimethyl phosphoryl butyrylcholine esterase and methyl phosphoryl butyrylcholine esterase by using antibody magnetic beads, and then carrying out enzymolysis by using pepsin to generate dimethyl phosphoryl nonapeptide and methyl phosphoryl nonapeptide, so as to obtain a nonapeptide solution to be detected; carrying out protein precipitation on the blood subjected to IMS treatment, wherein supernate is to-be-detected liquid of malathion and metabolite (malathion dihydroxy acid) of malathion; according to the method, malathion is taken as a template, protein precipitation is digested by streptavidin to generate dimethyl thiophosphoryl tyrosine, to-be-detected dimethyl thiophosphoryl tyrosine solutions are obtained, and the to-be-detected solutions are subjected to subsequent UPLC-MS detection, so that comprehensive monitoring of malathion at different levels and exposure conditions at different time points is realized, the method has a wide application prospect, and the detection method is high in sensitivity and high in precision.

The invention belongs to the technical field of pesticide detection, and particularly relates to a DNA dual-responsive nano-enzyme and a preparation method thereof, a detection kit containing the DNA dual-responsive nano-enzyme and application, the DNA dual-responsive nano-enzyme is carried out under low-temperature and low-pressure conditions, the problems of energy consumption, environmental pollution and the like caused by high-temperature and high-pressure treatment can be effectively avoided, meanwhile, the preparation method is high in operability, and the detection kit is suitable for industrial production. The method is environment-friendly; the compound has relatively strong peroxidase-like activity and can be widely used in the fields of biological catalysis and sensing detection; the prepared oxidized polypyrrole nano-enzyme has good responsiveness with a DNA sequence of a specific basic group; the detection kit containing the DNA dual-responsive nano-enzyme is used for detecting malathion and has the advantages of high specificity, high stability, low detection limit and the like, and the lowest detection limit is 0.0605 nM; the method has the characteristics of simple operation, no need of complex pretreatment of a to-be-detected sample, no need of expensive reagents, low detection cost, realization of portable detection and the like.