30 results about "Malathion" patented technology

The invention relates to a pesticide residue detection test strip as well as a preparation method and application thereof, in particular to a rapid detection technology based on an enzyme inhibition method and a chitosan non-woven fabric carrier. The test strip can specifically detect organophosphorus and carbamate pesticides such as trichlorfon, malathion and phoxim through the enzyme activity inhibition principle, the detection limit of the pesticides such as phoxim is as low as 0.1 mg / kg, the developing intensity is in negative correlation with the pesticide concentration, and semi-quantitative judgment can be achieved. The preparation process is simple, the chitosan non-woven fabric is adopted as a fixed carrier, the material is environment-friendly and degradable, the enzyme activity recovery rate reaches 90%, the stability is excellent (the enzyme activity recovery rate can be preserved for 60 days at the temperature of 4 DEG C in a dark place), sample pretreatment is not needed in detection operation, a result can be obtained at the normal temperature for 15 min, the stability and repeatability are good, and the problems that a traditional instrument is high in detection cost and long in time consumption are solved; the method has a remarkable basic popularization value.

The invention belongs to the technical field of environmental engineering, and discloses a microbial agent for degrading malathion as well as a preparation method and application of the microbial agent. The microbial agent comprises the following raw materials: compound bacteria, a nutritional supplement and a biomass carrier material, wherein the compound bacteria comprise functional bacteria and synergistic bacteria, and the mass ratio of the functional bacteria to the synergistic bacteria is (1.2-1.8): 1; the functional bacteria comprise issatchenkia orientalis, nocardia, brevibacillus laterosporus and bacillus cereus; the synergistic bacteria comprise azospirillum brasilense, bacillus thuringiensis and lactobacillus buchneri. Functional bacteria with direct degradation capacity and synergistic bacteria with auxiliary functions are creatively compounded according to a specific proportion, meanwhile, the garlic straw powder is selected as a biomass carrier material, all the raw materials complement each other in function and have a synergistic effect, the degradation rate and thoroughness of malathion are remarkably improved, and the degradation efficiency of malathion is greatly improved. The defects of low degradation efficiency and poor environmental adaptability of a single strain are overcome.

The invention belongs to the field of detection, and particularly relates to an electrochemical sensing platform for detecting lead ions and malathion as well as a preparation method and a detection method of the electrochemical sensing platform. The preparation method of the electrochemical sensing platform comprises the steps of designing a three-chain DNA compound, preparing a gold nanoparticle electrode, preparing the electrochemical sensing platform and the like. The electrochemical sensing platform realizes dual detection of lead ions (in a signal enhancement mode) and malathion (in a signal weakening mode) through specific recognition of DNAzyme and an aptamer on the basis of a three-chain DNA compound and a gold nanoparticle electrode. The method has the advantages of no label, high sensitivity (the detection limits respectively reach 5.42 pM and 0.34 pM), strong anti-interference capability and good stability, and is suitable for rapid detection of trace pollutants in environment and food samples.

The invention belongs to the technical field of sensor materials and malathion detection, and relates to a nano-silver modified covalent organic framework composite material, a preparation method and application thereof, and an electrochemical aptamer sensor. The preparation method comprises the following steps: S1, dissolving 1, 3, 5-tri (4-aminophenyl) benzene and 2, 5-divinyl-1, 4-phthalaldehyde in acetonitrile, adding a catalyst to carry out condensation reaction, standing, separating, washing and drying to obtain a covalent organic framework material; s2, dispersing the covalent organic framework material and silver nitrate in water, adding sodium citrate after ultrasonic dispersion, and washing and drying to obtain the nano-silver modified covalent organic framework composite material. The nano-silver modified covalent organic framework composite material prepared by the invention shows excellent crystallinity, extremely high porosity and good conductivity, and realizes high-sensitivity, rapid and accurate quantitative analysis of malathion in traditional Chinese medicinal materials.

The invention relates to the technical field of organic detection, in particular to a nanoparticle modified quantum dot MOF material and a preparation method and application thereof. A zirconium-based double-ligand metal organic framework material is used as a carrier, a co-reaction accelerant Ti3C2 quantum dot is modified to enhance the electrochemical luminescence reaction efficiency, and copper-gold nanoparticles are loaded to amplify signals. The nanoparticle modified quantum dot MOF material provided by the invention can be prepared into an aptamer biosensor for accurately identifying malathion, and has high sensitivity and high selectivity. According to the invention, a nanoparticle modified quantum dot MOF material is coupled and combined with an aptamer, and an aptamer probe with high electrochemical luminescence activity and specific sensitivity to malathion is constructed. When the malathion acts on the aptamer probe, the malathion is combined with the aptamer in a high affinity manner, the aptamer probe is separated from the surface of the electrode, and meanwhile, an electrochemical luminescence signal is changed, so that the malathion residue in the agricultural product is rapidly detected.

The invention discloses an extraction head for detecting malathion residues in agricultural products and a detection method applying the extraction head, the extraction head is a composite material of poly (5-amino indole pyrrole)-ionic liquid-porous nitrogen-doped graphene, and the ionic liquid is 1-butyl-3-methylimidazolium hexafluorophosphate. The extraction head has the beneficial effects that the extraction head made of the composite material has relatively good stability and repeatability, and can be used for rapidly detecting the residue of malafos sulfur in agricultural products.

This invention relates to the field of food safety testing technology and discloses an automatic matching method for batch parameters of malathion test cards with QR code traceability. The method includes collecting the dual-matrix Lab color space values ​​of the QR code label paper and NC film in the test card, constructing an aging decay function relationship table and an initial coupling correlation matrix, and encoding an encrypted QR code. During testing, a single frame of original image is acquired to extract real-time chromaticity feature vectors of the dual-matrix region, and the aging feature modulus is calculated by combining it with the initial coupling correlation matrix. Based on the aging feature modulus, the aging decay function relationship table is queried to dynamically correct the initial standard curve parameters and generate a real-time standard curve, thereby outputting the malathion concentration. This invention constructs an intrinsic aging indication mechanism through dual-matrix chromaticity difference, achieving adaptive reconstruction of the standard curve according to the aging state of the test card, ensuring detection accuracy throughout the entire lifecycle.

The invention discloses a method for multicolor detection of malathion based on Fe-Zr bimetallic nano-enzyme, a Fe-Zr bimetallic active center is formed, the synthesized Fe3O4-coated PDA-coated UiO-66 shows excellent peroxidase-like catalytic activity, the malathion can be combined with a composite material through a Zr-O-P bond, and the composite material can be used for detecting the peroxidase-like catalytic activity of the malathion, so that the malathion-coated UiO-66 can be used for detecting the peroxidase-like catalytic activity of the malathion-coated UiO-66, and the malathion-coated UiO-66 can be used for detecting the peroxidase-like catalytic activity of the malathion. By combining the Au NRs, multicolor detection of malathion is realized. The multicolor colorimetric detection method disclosed by the invention gets rid of the limitation that the traditional colorimetric method depends on different shade changes of a single color, so that the visual resolution is relatively poor, and the colorimetric signal can be combined with the smart phone, so that on-site instant detection is facilitated; meanwhile, a new way is provided for designing and applying the effective nano-enzyme to carry out pesticide field monitoring.

The invention relates to a method for detecting pesticide based on a ferriporphyrin modified covalent organic framework nano-enzyme sensing array. According to the invention, ferriporphyrin molecules are loaded on a porous and ordered COFs skeleton, so that excellent peroxidase simulated catalytic activity is realized, H2O2 can be catalyzed to be decomposed to generate reactive oxygen free radicals, and TMB, OPD and ABTS chromogenic reactions are catalyzed. Based on the performance, a three-channel colorimetric sensing array is constructed to detect and distinguish various pesticides such as malathion, profenofos, chlorpyrifos, acephate and dimethoate, and the detection limit is as low as 0.341 nM. After different pesticides are adsorbed to active sites, catalytic reaction can be remarkably inhibited, and specific colorimetric fingerprint signals are generated. Colorimetric data is further combined with a machine learning algorithm, high-accuracy classification and recognition of single pesticides and mixtures thereof are achieved by means of a multilayer sensor, a support vector machine, a random forest and other models, the classification accuracy of an MLP model reaches 90%, ROC curve AUC exceeds 0.97, and the reliability of the detection method is proved.

The invention belongs to the technical field of feed processing, and discloses a compound bacterium enzyme additive for improving the quality of corn green and yellow silage feed and application, the additive comprises a core functional group and an optional functional group; the core functional group consists of xylanase and lactobacillus plantarum, the activity of the xylanase is 100000Ug, and the addition amount of the xylanase is 0.25 g / kg; the activity of the lactobacillus plantarum is 1 * 10 CFUg, and the addition amount is 1mL / kg; the optional functional group is cellulase, the activity of the cellulase is 20000Ug, the addition amount of the cellulase is 0.1 g / kg, and the optional functional group is used for reducing malathion residues in the silage corn. The green / yellow silage treated by the method has the fragrance of aromatic wine, the stem and leaf structure is well kept, the color is consistent with that of raw materials, and the sensory score reaches 20 (superior grade) which is far superior to the medium grade of a control group.

The present invention discloses a molecular marker for malathion resistance of the oriental fruit fly and its application in malathion content detection. The molecular marker is the oriental fruit fly odor-binding protein BdorOBP28a-2 gene. Also disclosed are a primer pair, a kit, and a detection method for detecting the molecular marker: using an oriental fruit fly pesticide-sensitive population as a control, detecting the expression level of the OBP28a-2 gene of the oriental fruit fly to be tested; if the expression level of the OBP28a-2 gene of the oriental fruit fly to be tested is significantly higher than that of the sensitive population, it can be determined that the oriental fruit fly to be tested is a malathion-resistant population; if there is no significant difference in the expression level of the OBP28a-2 gene between the oriental fruit fly to be tested and the sensitive population, it cannot be determined that the oriental fruit fly to be tested is a malathion-resistant population. The present invention identifies and verifies for the first time that the oriental fruit fly odor-binding protein gene OBP28a-2 regulates the oriental fruit fly's resistance to malathion.

The invention discloses a preparation method and application of a pH-responsive multi-enzyme active fluorescent nano-enzyme, and belongs to the technical field of nano-enzyme preparation and application research. The multi-enzyme active fluorescent nano-enzyme is prepared by taking D-tryptophan or L-tryptophan, silver nitrate and chloroauric acid as raw materials and adopting a heating reflux method, and the preparation process is simple and convenient to operate, green, mild, stable and efficient. The prepared multi-enzyme active fluorescent nano-enzyme not only has strong blue fluorescence, but also shows excellent multi-enzyme activity, including oxidase, laccase and catecholase, and switching of the three enzyme activities can be realized by adjusting pH. In addition, the method is applied to quantitative detection of malathion and 2, 4-dichlorophenol by utilizing the characteristics of different enzyme activities.

The present invention relates to an improved synthesis of malathion. The presence of an acid facilitates the reaction between O,O-dimethyldithiophosphoric acid (O,O-DMDTPA) and maleate, leading to excellent product yield in a shorter reaction time and with fewer impurities.

The invention belongs to the field of environment detection, and particularly relates to application of bimetal Pt / Co nano-enzyme to instant portable detection of malathion. The bimetallic Pt / Co nano-enzyme for malathion detection is ZIF-67NF loaded with Pt nanoparticles and has peroxidase activity, nucleotide can enhance the peroxidase activity of the bimetallic Pt / Co nano-enzyme under an alkaline condition, a colorimetric reaction is started by combining the inhibition effect of malathion on alkaline phosphatase and the decomposition effect of the alkaline phosphatase on the nucleotide, and the sensitivity of malathion detection is improved. The method is used for detecting malathion, has the advantages of low detection cost, instantaneity, accuracy and the like, and provides a new method for detecting malathion.

The invention belongs to the technical field of pollutant detection, and relates to a Fe-MOF / Fe3S4 composite material and an application of the Fe-MOF / Fe3S4 composite material as a nano enzyme in malathion detection. The preparation method comprises the following steps: mixing a ferric trichloride hexahydrate solution and a fumaric acid solution to obtain a mixed solution A, and then carrying out a reflux reaction to obtain an Fe-MOF precursor; and adding a sulfur source into an ethanol dispersion liquid of the Fe-MOF precursor, carrying out ultrasonic treatment, sealing, and carrying out a hydrothermal reaction to prepare the Fe-MOF / Fe3S4 composite nanomaterial. The preparation method provided by the invention is simple and convenient in process, low in raw material price, rich in resource, low in synthesis cost, stable in product performance and suitable for large-scale synthesis. Visual detection of malathion can be realized through color change of the solution, and the method has the advantages of high sensitivity, low detection limit, wide linear response range and good selectivity and stability.

The invention discloses a method for preparing malathion by adopting a solid acid catalyst, and belongs to the technical field of organophosphorus pesticide synthesis. O, O-dimethyl dithiophosphoric acid and diethyl maleate are used as raw materials, solid acid is used as a catalyst, and addition reaction is performed to prepare malathion; the solid acid is selected from one or more of ethylenediaminetetraacetic acid, sulfonated carbon, sulfate zirconium oxide and silicon-aluminum oxide. The process provided by the invention breaks through the technical bottleneck of catalyst selection in malathion synthesis, solves the environmental protection pain point and the efficiency bottleneck in the prior art through innovative application of specific solid acid, and has the advantages of recoverable catalyst, high raw material conversion rate, excellent product purity, simple process, low energy consumption and the like. The method does not need to greatly transform the existing industrial production device, can be directly popularized and applied, can effectively reduce the production cost, reduce the environmental pollution and improve the product competitiveness, is suitable for large-scale production of agricultural, hygiene and epidemic prevention and medicinal malathion, and has wide industrial application prospects and remarkable economic, social and environmental benefits.

The invention discloses a dual-mode electrochemical / colorimetric aptamer sensor for detecting malathion in vegetables. The sensor is combined with magnetic Fe3O4 (at) ZIF-8-DNA (deoxyribonucleic acid) and CuZr-MOF-cDNA (complementary deoxyribonucleic acid), and a target object can be detected through electrochemical reaction and colorimetric change. The CuZr-MOF not only improves the electrochemical detection sensitivity, but also can catalyze the chromogenic reaction to realize colorimetric detection. The magnetic Fe3O4 ZIF-8 realizes solid-liquid separation under the action of an external magnetic field, and the sensor has a wider detection range due to the large specific surface area of the magnetic Fe3O4 ZIF-8. When the target malathion exists, the aptamer is combined with the target malathion, CuZr-MOF-cDNA is released, colorimetric signals with different intensities are generated, and meanwhile, the target in the precipitate can be quantitatively detected through an electrochemical method. Experimental results show that a colorimetric signal and an electrochemical signal have a good linear relationship. The sensor is high in sensitivity, good in selectivity and suitable for on-site rapid detection.

The application discloses a time sequence control type composite pollution repair material for facility agriculture soil and a preparation method and application thereof, and belongs to the technical field of soil repair. The repair material is a three-layer core-shell structure particle, which comprises, from inside to outside, an inner core layer composed of biodegradable polyester wrapping pesticide degrading enzyme and pesticide adsorbent, a middle layer composed of water / ion responsive hydrogel wrapping heavy metal fixing agent and oxidative degradation catalyst, and an outer shell layer composed of brittle degradable polymer wrapping micro / nano plastic surface capping agent. The repair material realizes time sequence and synergistic repair of micro / nano plastic, available Cd and malathion by triggering the release of the outer shell layer through plowing mechanical force, triggering the slow release of the middle layer through irrigation water response, and triggering the release of the inner core layer through rhizosphere microbial enzyme. The material has simple preparation process, convenient application and good application prospect.

This invention provides the application of Fe / C composite material combined with *S. wanandii* in the degradation of the pesticide malathion, belonging to the field of pesticide degradation technology. This invention provides the application of *S. wanandii* in the degradation of the pesticide malathion, offering a new method for the biodegradation of malathion. This invention also provides a material for degrading the pesticide malathion, comprising an Fe / C composite material and *S. wanandii* loaded within the pores of the Fe / C composite material. The porous structure of the Fe / C composite material serves as a habitat for *S. wanandii*. Scanning electron microscopy reveals clusters of deposits on the spiral vessels of the Fe / C composite material, within the vascular bundles, and within the pits on the radial and transverse walls of the fibrous cell cavities. *S. wanandii* colonizes and grows within these pores, and the Fe / C composite material enhances the degradation of malathion by *S. wanandii*.

This invention belongs to the field of food safety testing technology, and relates to a porous zirconium-based nanozyme and its application in the detection of malathion. Tetra(4-carboxyphenyl)porphyrin and zirconium oxychloride octahydrate are added to N,N-dimethylformamide and mixed thoroughly; then, a hydrothermal reaction is carried out with glacial acetic acid solution to obtain PCN-224 spheres. These spheres are then added to an ethanol solution and mixed thoroughly, followed by the addition of chloroplatinic acid solution and sodium borohydride solution, and then stirred to obtain PCN-224@Pt. The material synthesized by this invention has a mesoporous structure of porous nanospheres, the raw materials are inexpensive, the performance is stable, and it is suitable for large-scale synthesis. It enables the visual detection of malathion.

The application provides a beta-cyclodextrin modified electrochemiluminescence gold cerium bimetallic nanocluster, a preparation method and application thereof in electrochemiluminescence-impedance dual-mode detection of malathion, and relates to the field of malathion detection. The preparation method of the beta-cyclodextrin modified electrochemiluminescence gold cerium bimetallic nanocluster comprises the following steps: after gold source and cerium source are incubated with methionine for the first time in an alkaline environment, the gold source and the cerium source are dispersed in an ammonia solution to be incubated for the second time; and finally, the gold source and the cerium source are incubated with NH2-beta-cyclodextrin for the third time to obtain the beta-cyclodextrin modified electrochemiluminescence gold cerium bimetallic nanocluster. The beta-cyclodextrin modified electrochemiluminescence gold cerium bimetallic nanocluster is used as an ECL luminophore, and a sensor using triethylamine as a co-reaction agent is applied to the detection of malathion, and the sensor has high sensitivity, selectivity, stability, realizes ECL and EIS dual-mode cooperative detection and mutual verification, and has the advantages of simple operation and good repeatability.

The application provides a beta-cyclodextrin modified electrochemiluminescence gold cerium bimetallic nanocluster, a preparation method and application thereof in electrochemiluminescence-impedance dual-mode detection of malathion, and relates to the field of malathion detection. The preparation method of the beta-cyclodextrin modified electrochemiluminescence gold cerium bimetallic nanocluster comprises the following steps: after gold source and cerium source are incubated with methionine for the first time in an alkaline environment, the gold source and the cerium source are dispersed in an ammonia solution to be incubated for the second time; and finally, the gold source and the cerium source are incubated with NH2-beta-cyclodextrin for the third time to obtain the beta-cyclodextrin modified electrochemiluminescence gold cerium bimetallic nanocluster. The beta-cyclodextrin modified electrochemiluminescence gold cerium bimetallic nanocluster is used as an ECL luminophore, and a sensor using triethylamine as a co-reaction agent is applied to the detection of malathion, and the sensor has high sensitivity, selectivity, stability, realizes ECL and EIS dual-mode cooperative detection and mutual verification, and has the advantages of simple operation and good repeatability.

The invention belongs to the technical field of food safety detection, and particularly relates to an electrochemical luminescence sensor and application of the electrochemical luminescence sensor in multiple detection of malathion and phorate. In order to detect a target object with high sensitivity and high selectivity, the preparation method comprises the following steps: firstly, synthesizing Co-PTC MOF by taking perylene tetracarboxylic acid potassium as a ligand, and preparing an Ag-Co-PTC composite material on the surface of the Co-PTC MOF through photo-reduction of AgNO3; the electrochemical luminescence biosensor based on the Ag-Co-PTC composite material is constructed by taking Ag-Co-PTC as an electrochemical luminescence signal probe and combining CRISPR / Cas12a and a spherical nucleic acid conversion technology, so that the dual detection of malathion and phorate is realized, and the detection limits are 1.08 * 10 <-13 > M and 1.01 * 10 <-12 > M.

The invention belongs to the technical field of pesticide detection, and particularly relates to a DNA dual-responsive nano-enzyme and a preparation method thereof, a detection kit containing the DNA dual-responsive nano-enzyme and application, the DNA dual-responsive nano-enzyme is carried out under low-temperature and low-pressure conditions, the problems of energy consumption, environmental pollution and the like caused by high-temperature and high-pressure treatment can be effectively avoided, meanwhile, the preparation method is high in operability, and the detection kit is suitable for industrial production. The method is environment-friendly; the compound has relatively strong peroxidase-like activity and can be widely used in the fields of biological catalysis and sensing detection; the prepared oxidized polypyrrole nano-enzyme has good responsiveness with a DNA sequence of a specific basic group; the detection kit containing the DNA dual-responsive nano-enzyme is used for detecting malathion and has the advantages of high specificity, high stability, low detection limit and the like, and the lowest detection limit is 0.0605 nM; the method has the characteristics of simple operation, no need of complex pretreatment of a to-be-detected sample, no need of expensive reagents, low detection cost, realization of portable detection and the like.

The invention provides a method and device for continuously producing dimethoate, and relates to the technical field of continuous and efficient pesticide and medicine production.The device comprises a dimethoate continuous reaction kettle, and a stirring frame is integrally composed of a vertical stirring column and a row of horizontal stirring rods welded to the horizontal stirring rods at equal intervals; the head end part of the horizontal stirring rod is inserted and matched with the peripheral wall of the hollow rotating shaft in a penetrating manner; a plurality of circles of rectangular positioning frames arranged in the axial direction are welded to the inner circle of the peripheral wall of the hollow rotating shaft. The head end part of the horizontal stirring rod is inserted between the two vertically adjacent rectangular positioning frames at the corresponding height, and the vertical insertion plate is in penetrating sliding fit with the two vertically adjacent rectangular positioning frames at the corresponding height and is in penetrating insertion fit with a rectangular positioning groove formed in the head end part of the horizontal stirring rod in a penetrating manner. According to the invention, the trouble of locking and unlocking the stirring frame before and after maintenance or replacement due to additional manual up-and-down sliding of the six-edge driving shaft can be avoided, and the maintenance and replacement efficiency of the stirring frame can be indirectly improved.

The invention discloses a method for preparing a polymerized 6-bromoindole-IL-aminated graphene octadecylamine modified-nitrogen doped graphene-multi-walled carbon nanotube coating on the surface of a stainless steel wire by utilizing a cyclic voltammetry method. A method for detecting malathion residues in fresh vegetables or fruits and a method for detecting phenolic compounds in a water environment are constructed by adopting a headspace extraction technology and combining gas chromatography-mass spectrometry (GC-MS). The established analysis method has good linear response to phenolic substances and malathion, and the established analysis method is used for detecting malathion residues in fresh vegetables or fruits and analyzing phenolic compounds in water samples. Wherein the adding standard recovery rate of the pear synthase water sample is between 99.6% and 100.3%, and the adding standard recovery rate of the pear synthase water sample is between 99.7% and 100.3%, so that the method has relatively good practicability.

A novel electrochemiluminescence (ECL) aptamer biosensor based on functional nanomaterials was developed for the detection of malathion in tea. Firstly, multi-walled carbon nanotubes (MWCNTs) were used to immobilize gold nanoparticles (AuNPs) on the surface of the electrode by potentiostatic electrodeposition. Secondly, amino-luminol / silver nanoparticles@silica nanospheres (NH2-Luminol / Ag@SiO2NSs) were synthesized by one-pot method. Finally, the signal probes (SPs) were prepared by combining NH2-Luminol / Ag@SiO2NSs with malathion aptamer. Under the optimized conditions, the biosensor showed a wide linear range (1´10 ‑3 3 ng / mL) and a low detection limit (0.3×10 ‑3 ng / mL). The aptamer biosensor was sensitive, stable and specific, and could be used for the detection of malathion in tea. This study provided an effective method for the construction of aptamer biosensor with rapid, high sensitivity and low cost.​

The invention relates to a method for rapidly identifying malathion based on a fluorescent nanoprobe, which comprises the following steps: step 1, preparing a Tris-HCl solution, an HP solution, an Aptamer solution, a chloroauric acid solution and a trisodium citrate solution for later use; 2, preparing a gold nanoparticle solution for later use through the chloroauric acid solution and the trisodium citrate solution prepared in the step 1; step 3, preparing the fluorescent nano-probe from the Tris-HCl solution, the HP solution and the Aptamer solution prepared in the step 1 and the nano-gold particle solution prepared in the step 2; and step 4, identifying malathion through the fluorescent nano probe. Compared with a traditional malathion analysis method, the method has the beneficial effects that the steps of sample treatment are simplified, the operation is simple, the identification time of pesticide malathion is saved, the accuracy is high, and the prepared nano fluorescent probe is easy to purify, good in selectivity and high in sensitivity. The advantages of simple operation and the like of a spectrum method are reserved in the operation of analyzing and identifying malathion, and the method is more beneficial to popularization and application.

The application relates to a foldable multi-channel paper-based microfluidic chip and a preparation method and application thereof. The paper-based microfluidic chip comprises a plurality of micro-detection channels. The multi-channel detection capacity enables the microfluidic paper-based chip to simultaneously detect a plurality of pollutant items. The application also discloses application of the paper-based microfluidic chip in simultaneous detection of environmental endocrine disruptors, i.e. bisphenol A, 17beta estradiol and malathion, in pretreated landfill leachate by a solid-phase microextraction technology in combination with a nano-mixed material. The paper-based microfluidic chip has been successfully applied to detection and analysis of environmental endocrine disruptors, and can be completed in a short time only by using a small amount of reaction reagents and samples, and has the characteristics of rapidity, convenience, systematic identification, economy and high efficiency. According to the structure of target analytes, corresponding aptamers can be screened and applied, and a new idea is provided for analysis of other environmental endocrine disruptors or environmental pollutants, and the application has important significance for environmental risk assessment, pollutant control and public health.

The application discloses a dimethoate molecular imprinting photoelectrochemical sensor based on a multi-center affinity MOF, takes dimethoate (DIM) as a target analyte, and uses PCN-224 (Zn) as not only a photoelectric signal generation center of the sensor, but also a matching center of a molecular imprinting cavity, so that multiple recognitions are performed on the target, and the affinity effect of Lewis acid center sites (Zn (II), Zr (IV)) and the synergistic effect of spatial matching of the molecular imprinting ensure accurate capture of the DIM; through a competition strategy of taking dopamine as an electron donor and an interface probe, a high-selectivity PEC sensor for trace DIM is finally constructed, and the method can be popularized to other organophosphorus chemical structures, thereby providing a new direction for multifunctional application of photoactive MOFs.