The invention relates to a multifunctional mouse tail intravenous injection platform. The mouse tail intravenous injection platform comprises a platform body, a mouse cage, a pressure regulating device, a mouse tail bracket, a temperature control device, a light guide system device and a magnifying lens; the mouse cage is fixed to the upper surface of the platform body; the pressure regulating device is arranged at the front end of the mouse cage, and the mouse tail bracket is arranged on the outer side of the pressure regulating device; the temperature control device is arranged on one side of the mouse tail bracket; the magnifying lens is arranged at the edge of one end of the mouse tail bracket. The multifunctional mouse tail intravenous injection platform has the advantages that pressure regulation can be conducted on the root of a mouse tail, and under a pressurization state, the intravenous distal lumen of the mouse tail can be expanded, so that decompression is facilitated, quick and stable caudal intravenous puncture and injection are facilitated, and manpower and cost can be reduced; the mouse tail can be heated, and the temperature can be controlled and adjusted to 35 DEG C, so that the tail vein is expanded; meanwhile, through the light guide system device, the magnifying lens and other auxiliary devices, the visual resolution of an operator is improved, and the success rate of puncturing is increased.