25 results about "Tail vein" patented technology

This utility model relates to a simple experimental mouse fixation and tail vein injection device, including a fixation platform, a transparent isolation cover, and a tail fixation component. The transparent isolation cover is used to cover the experimental mouse on the fixation platform. An isolation cover notch is formed at the edge of the open end of the transparent isolation cover, through which the tail of the experimental mouse protrudes. The tail fixation component is used to fix the protruding tail of the experimental mouse on the fixation platform. This utility model combines experimental mouse fixation, tail fixation, and visual monitoring, achieving a dual improvement in drug administration accuracy and simplified experimental operation. It can be operated by a single person and is suitable for precise drug delivery and real-time image monitoring in small animal experiments, possessing high practical value and promising prospects for widespread application.

The invention relates to the field of constant-temperature heating, and particularly discloses an automatic constant-temperature heating system for tails of rats and mice, which comprises a temperature sensing module for collecting tail temperature data in real time, an environment compensation module for generating heating compensation parameters based on a Fourier transform model, and dynamically adjusting the power of the heating module; the intelligent clamping module recognizes the diameter of the tail and automatically adapts to and fixes the tail and the body of the mouse; the whole-body heat preservation module is used for maintaining mouse body temperature; and the energy storage power supply module is used for storing and supplying electric energy. By self-adaptive environment compensation and heating power adjustment, accurate temperature control is realized, experimental efficiency and data reliability are remarkably improved, animal stress response is reduced, and the experimental device is suitable for experimental requirements of tail vein injection.

The utility model discloses a caudal vein injection device, relates to the technical field of mouse caudal vein injection devices, and aims to solve the problems that the stability of the existing mouse caudal vein injection device, a mounting plate and a transparent fixing barrel are of an integrated structure, but the tail end of a mouse is positioned at the joint of the mounting plate and the transparent fixing barrel during injection; the mouse is easily frightened to excrete in the device during injection, and the part is difficult to clean after injection is completed. A transparent fixing barrel is mounted in the middle of one side of the mounting plate, a plugging mechanism is movably mounted in the transparent fixing barrel, locking hooks are symmetrically and fixedly arranged on the upper portion and the lower portion of the side, close to the mounting plate, of the transparent fixing barrel, and locking hook grooves are symmetrically formed in the mounting plate; the locking hook groove and the locking hook are correspondingly arranged, a sliding groove is formed in the front side of the transparent fixing barrel, a locking bolt groove is formed in the rear side of the transparent fixing barrel, and a mouse tail through groove directly communicated with the edge is formed in the center of one side of the mounting plate.

The present invention discloses a method for constructing an animal model of glioma based on AAV-mediated immune microenvironment, comprising: selecting immunodeficient mice of appropriate age; injecting human CD34+ hematopoietic stem cells for immune system reconstruction; detecting human CD45+ immune cells in peripheral blood PBMC; selecting tumor tissues that highly express glioma marker genes, performing rapid qPCR or targeted RNA-seq immunohistochemistry multiple verification, and screening tumor tissues that meet the requirements; physically cutting the tumor tissue and treating it with a pretreatment solution, digesting it into a single cell suspension, and inoculating it into the mouse's skull; injecting an AAV vector carrying a specific immune regulatory gene into the mouse's tail vein to regulate the glioma immune microenvironment; when the tumor tissue is observed to grow stably and have immune cell infiltration characteristics, the construction of the glioma animal model is completed. Compared with existing models, the present invention provides a more stable, more scientific, and more human immune background glioma model, which is suitable for related immunotherapy drug screening and mechanism research.

The invention discloses an application of an alkyl acid compound in preparation of a medicine for regulating ethanol autonomous intake preference. The invention also discloses a pharmaceutical composition for adjusting the ethanol autonomous intake preference, and the pharmaceutical composition comprises the alkyl acid compound or the pharmaceutically acceptable derivative thereof, and a pharmaceutically acceptable carrier and / or excipient. The application of the alkyl acid compound in preparing the medicine for adjusting the ethanol autonomous intake preference is provided for the first time, and animal experiments show that single injection of propofol into the tail vein of a mouse can continuously inhibit the ethanol autonomous intake preference of the mouse. The active ethanol intake absolute amount of mice and the relative ethanol intake proportion relative to the water intake amount can be obviously reduced, and the inhibition effect can last for at least 6 days.

This utility model discloses an auxiliary device for tail vein injection in medical experimental mice. Relating to the technical field of medical experimental equipment, the device includes a base with a fixing frame installed near the edge of the top. A fixing component is provided on the top of the base, and an observation component is provided on one outer wall of the fixing frame. The fixing component includes a lower fixing plate and an upper fixing plate, with the upper fixing plate positioned above the lower fixing plate. This auxiliary device for tail vein injection in medical experimental mice allows the upper fixing plate to move upward a certain distance by pulling a lever, overcoming the spring's thrust, and completely separating the upper and lower arc-shaped plates. The mouse is then placed on the lower arc-shaped plate. Releasing the lever causes the upper arc-shaped plate to move downward under the spring's thrust, thus securing the mouse and effectively avoiding the problem of mice being difficult to stabilize due to their active and restless nature.

The invention relates to an elastin-like polypeptide-based composite material and a cell membrane bionic elastin-like polypeptide-based nano-drug. After the nano-drug is injected into the body of a mouse through caudal vein, the nano-drug can be promoted to cross a blood brain barrier to enter in-situ brain glioma, meanwhile, MR imaging, chemical kinetic treatment and sonodynamic treatment of tumors are achieved, and by activating a cGAS-STING pathway, immunogenic cell death is caused to activate anti-tumor immune response, so that the anti-tumor effect is achieved. The obvious anti-tumor effect and the potential clinical application value are realized.

The invention relates to a mitochondrial epilepsy mouse disease model and a construction method thereof, which are applied to mitochondrial epilepsy targeted therapy. The PHP.eB-UL12.5 is injected through caudal veins, blood brain barriers can be effectively permeated, mitochondrial genes of mouse brain tissue are cut, mitochondria of the brain tissue loses functions, an epilepsy mouse model induced by mitochondrial DNA deletion is obtained, and the epilepsy mouse model is high in induction success rate, safe, efficient and good in repeatability.

The utility model belongs to the field of animal experiments, and particularly relates to an experimental mouse fixing device which comprises a weighing scale, a frame and an adjusting mechanism. Wherein the frame is arranged on the weighing scale, the frame is provided with a transversely-arranged grabbing rod, the top of the frame is provided with a top cover, the top cover is provided with an opening, the grabbing rod is located below the opening, and at least one face of the frame is provided with a plate body; the adjusting mechanism is used for adjusting the size of the opening, the front claw of the mouse grabs the grabbing rod, the head of the mouse is led out from the opening, then the linear reciprocating adjusting piece is operated to drive the four movable plates to enable the area of the surrounding area to be gradually reduced until the head of the mouse is controlled, then the tail of the mouse is led out from the through hole of the plate body, and the weight information of the mouse can be obtained through the weight scale. An operator only needs to slightly pull the scalp of a mouse to carry out gavage and carry out caudal vein injection operation, so that the experimental animal does not need to be fixed and loosened for multiple times, then gavage, weight measurement and caudal vein injection operation can be continuously carried out, and the operation process is greatly simplified.

This invention discloses a method for constructing a mouse model of non-obese NAFLD in humans under normal dietary conditions. The method involves injecting adeno-associated virus (adenovirus) shIDO2-AAV8 into mice via the tail vein to target and silence IDO2 gene expression in the liver, while simultaneously administering a normal diet. The adenovirus shIDO2-AAV8 is obtained by recombining an IDO2-shRNA sequence inserted into an AAV8 vector. This invention, through tail vein injection of adenovirus shIDO2-AAV8 combined with a normal diet, can construct a mouse model simulating non-obese NAFLD in humans. Compared with traditional dietary induction methods and combined induction methods, the dietary conditions are consistent with daily human intake, more closely reflecting the actual pathological situation in humans, and more closely reproducing the pathogenesis environment and physical state of non-obese NAFLD in humans, making the model more clinically valuable.

The utility model discloses a mouse caudal vein injector, which belongs to the technical field of mouse caudal vein injection and comprises a base, an inclined bearing plate is fixed at the upper end of the base, a fixed sleeve is arranged at the upper end of the bearing plate, and the bottom end of the fixed sleeve is connected with an injection plate. According to the mouse tail combing device, the combing assembly composed of the guide groove, the guide block, the combing block, the pressing plate, the pressing rod, the bearing base, the rolling shaft and the spring is designed, in the process of combing the tail of a mouse, the mouse penetrates through the combing groove, then external force is applied to the pressing plate, and the pressing plate drives the rolling shaft to move downwards to press the tail; meanwhile, a carding block is pushed to move along the injection plate under the action of a guide block and a guide groove, the tail can be carded and straightened while being pressed, and the tail is continuously pressed after being carded, in this way, workers can apply external force conveniently, then the pressing effect on the tail is improved, smooth injection is guaranteed, and the using effect of the device is improved.

The invention belongs to the technical field of medicines, and particularly relates to application of CD47 as a target spot in treatment of heat stroke. The invention provides application of CD47 in preparation of a medicine or a medicine composition for treating heat stroke. The CD47 is used as a heat stroke treatment target for the first time, and a remarkable treatment effect is achieved. In a heat stroke mouse model, the function damage of multiple organs such as lung, kidney and the like caused by heat stroke can be relieved by injecting the CD47 monoclonal antibody into the caudal vein, the survival rate of the heat stroke mouse is increased, and the survival rate can be increased to about 80% by applying the CD47 monoclonal antibody under the dosage of 10 mg / kg. Therefore, the CD47 can be used as a therapeutic target for preparing the medicine for treating the heat stroke.

The application relates to a method for establishing a systemic amyloidosis model and application, a systemic amyloidosis model is established by injecting amyloidosis lysozyme into the tail vein of a mouse to induce amyloidosis of the mouse tissue, the systemic amyloidosis model obtained can simulate the pathological state of systemic amyloidosis in the body, is used for activity screening of amyloidosis prevention and treatment drugs, and has a good application prospect in the treatment and prevention of amyloidosis.

The invention particularly relates to application of myrobalan tannic acid in preparation of a medicine for treating respiratory syncytial viruses. The administration dosage of the myrobalan tannic acid ranges from 0.016 mg / kg to 0.05 mg / kg. The myrobalan tannic acid disclosed by the invention has the effects of resisting RSV (Respiratory Syndrome Virus) and relieving inflammation on mice in a tail vein injection manner.

The invention relates to an AAV (adeno-associated virus) virus for knocking down CTH (cytidylcholine) in targeted hepatocytes and application thereof, which adopts an adeno-associated virus vector system driven by a TBG promoter to realize specific CTH gene knocking down of the hepatocytes through caudal vein injection. Experiments prove that the targeted knock-down hepatocyte CTH can significantly improve the liver function index of an OC mouse model, relieve inflammatory response and reverse the fibrosis process. The key regulation and control effect of the CTH gene in the OC process is disclosed for the first time, an ideal preclinical research model is provided for developing a CTH-targeted OC treatment medicine, and the application has important application value in the aspect of improving the prognosis of a patient.

The invention belongs to the technical field of biology, and provides an IgM monoclonal antibody for resisting ox-LDL phagocytosis of macrophages, and the IgM monoclonal antibody is respectively named as 1B4, 2B4, 2H8 and 4D3. The four IgM monoclonal antibodies have a good inhibition effect on foam cells formed by phagocytosis of ox-LDL by macrophages, and the IgM antibodies injected through tail vein can significantly inhibit formation of atherosclerotic plaques in a mouse body, and can be used for prevention and treatment of atherosclerosis and atherosclerotic cardiovascular and cerebrovascular diseases caused by atherosclerosis.

The utility model belongs to the technical field of animal medicine experiment instruments, and discloses a combined type fixing device for experimental rat knee joint injection, which comprises two combined type sleeves, a fixing knob, a clamping groove module, a sliding groove, a bottom supporting plate and two adjusting sliding doors. The fusiform closing opening of the front sleeve can fix the head of a rat, the top of the rear sleeve is provided with a groove, the two sides of the bottom of the rear sleeve are provided with openings, the sliding door is adjusted, the tail and the lower limbs of the rat can be fixed, and the front sleeve and the rear sleeve are combined and separated through a fixing knob. The device is especially suitable for knee joint injection molding of experimental rats with different body types, and is also suitable for experiments such as tail injection and caudal vein blood collection. The experimental rat fixing device is environment-friendly in material, low in price, easy to manufacture and convenient to use, efficiency and safety of rat knee joint injection molding can be improved, and the defects of an existing experimental rat fixing device are overcome.

The invention discloses a mouse model construction method for simulating intravenous injection circulating tumor cells, and belongs to the field of cell model construction. The invention discloses a method for constructing a tail vein injection circulating tumor cell mouse model for simulating lung metastasis of breast cancer. The method comprises the following steps: S1, treating a tumor operation specimen before transplantation; s2, constructing a PDX model mouse; s3, obtaining a circulating tumor cell suspension; s4, transplanting the circulating tumor cell suspension into the renal capsule of the immunodeficient mouse; s5, obtaining a tail intravenous injection circulating tumor cell mouse model for simulating the lung metastasis of the breast cancer; and S6, monitoring, verifying and evaluating the model. The problems that in the prior art, sources of circulating tumor cells are mixed, and deviation exists between the circulating tumor cells and actual conditions in clinic are solved, the goodness of fit between the circulating tumor cells and the clinical metastasis process is greatly improved, the number of metastases can be precisely regulated and controlled, and the reliability of data and the effectiveness of statistical analysis are ensured.

The application relates to the field of biological medicine, and discloses a medicine for relieving autoimmune prostatitis and application thereof, which comprises an active ingredient and a pharmaceutically acceptable sterile phosphate buffer carrier; the active ingredient is live Lactobacillus johnsonii with a number of BNCC135265 or extracellular vesicles of Lactobacillus johnsonii; the final concentration of live bacteria in the medicine is 1x10 8 to 1x10 10 CFU / mL, which is used for preparing a preparation for intragastric administration; the extracellular vesicles contain 1x10 8 to 5x10 9 particles per unit dosage form, which is used for preparing a preparation for tail vein injection administration. By using the live bacteria with the specific number or the extracellular vesicles thereof, intestinal mucosa colonization can be achieved to block endotoxin into blood, or the extracellular vesicles can be enriched in prostate lesions to inhibit a nuclear factor-kappa B inflammatory signaling pathway, reduce synthesis of proinflammatory cytokines and lymphocyte infiltration, and finally realize relief of autoimmune prostatitis.

The present invention discloses a method for preparing MHCII-positive exosomes rich in antigen presenting molecules, and belongs to the field of biotechnology. The method of the present invention comprises the following steps: after the Vibrio anguillarum is shaken to the logarithmic growth phase, it is inactivated; the inactivated Vibrio anguillarum solution is taken for intraperitoneal injection into fish, and the induction is 6-24h; after the fish is anesthetized, peripheral blood is extracted along the tail vein with a syringe; after the peripheral blood is removed, serum-free L15 culture medium is injected into the peritoneal cavity of the fish with a syringe; after massaging the fish abdomen, the peritoneal fluid is extracted along the upper edge of the abdominal cavity with a syringe; finally, the peritoneal fluid is separated by a continuous gradient centrifugation method to obtain MHCII-positive exosomes. The exosomes rich in antigen presenting molecules MHCII-positive prepared by the present invention have a significant effect in promoting T cell activation and vaccine immune efficacy. The present invention creatively establishes a method for preparing fish-rich antigen presenting molecules MHCII-positive exosomes with high efficiency, high purity and high yield through stimulants suitable for fish and a unique fish peritoneal lavage method, which has important scientific significance and application value.

The present disclosure belongs to the technical field of biological medicine, and relates to an mRNA composition and application thereof, wherein the mRNA composition comprises a lipid nanoparticle and mRNA contained in the interior of the lipid nanoparticle, and the mRNA can express CXCL3. After the mRNA composition of the present disclosure is infused through the tail vein, a large number of monocyte-derived macrophages can be chemotactically infiltrated into the cirrhotic liver of a mouse, and the macrophages can phagocytize fibrosis, thereby significantly reducing the fibrosis level in the liver of the mouse. Experimental results show that the composition has almost no toxicity to normal tissues, has high safety, and shows potential in treating liver fibrosis.

The application discloses a kind of methods for constructing deep vein thrombosis mouse model by regulating Pyk2 gene expression.The method comprises the following steps: constructing knockdown or overexpression vector for Pyk2 gene; by tail vein injection, the vector is introduced into mouse in vivo, the in vivo transient regulation of Pyk2 gene is realized; the inferior vena stenosis method is used to induce deep vein thrombosis; the thrombus macroscopic morphology, wet weight, length and histopathology are detected.The application overcomes the limitation of existing gene knockout model, such as congenital, irreversible and systemic deletion, realizes the targeted, temporary and bidirectional flexible regulation of Pyk2 gene in vascular tissue, and the model has high stability and good repeatability.The model can simulate the pathological state under the same expression level of Pyk2, and is suitable for the screening, evaluation and deep vein thrombosis pathogenesis research of antithrombotic drugs.

The invention discloses a device for assisting tail vein injection of C57BL / 6 mice. The device comprises a bottom plate, an external cavity, an internal accommodating cavity and an adjusting plug plate. The external cavity is fixed above the bottom plate through supporting feet, the internal accommodating cavity is arranged in the external cavity, and the adjusting plug plate is arranged at the front side of the internal accommodating cavity. The device has a simple structure and is convenient to operate. The spatial position of mice in an experimental state can be quickly and efficiently restricted, and subsequent operations are convenient. The device avoids adverse effects on the experiment caused by stress reaction of mice due to directly grabbing animals for experiments to the greatest extent. In combination with the use of an infrared light source, the tail vein injection process is visualized, and no physical damage is caused to the mice or chemical reagents are caused to penetrate into the mice, thereby further ensuring the accuracy of the experimental results.

The application relates to the field of molecular biology and biomedicine technology, and particularly discloses application of Metrnl as a myocardial infarction first-aid medicine. After a mouse is subjected to myocardial ischemia / reperfusion modeling and then treated by Metrnl protein injection through a tail vein, it is found that the treatment of the Metrnl protein can improve the cardiac function in the first, second and third days after myocardial ischemia / reperfusion, improve the myocardial infarction area in the first day, and improve the mortality of the mouse within 7 days. It is shown that the Metrnl can be used as the myocardial infarction first-aid medicine, and has important significance in the treatment of myocardial infarction diseases.

The present invention discloses a method for constructing a Parkinson's disease model in tree shrews, comprising the following steps: (1) weighing ceramide and dissolving it in sterilized ultrapure water to prepare a solution with a concentration of 0.6 mg / mL; (2) administering 4 μg / g of ceramide to the tree shrews through the tail vein once a day for 7 consecutive days, and then raising the tree shrews normally for 2 weeks after the administration to complete the modeling. In this application, a stable Parkinson's disease model can be established in tree shrews by injecting ceramide into the tail vein at a dose of 4 μg / g for 1 week and then raising the tree shrews normally for 2 weeks after the administration.