Construction method of systemic lupus erythematosus animal model and application thereof

A technology of lupus erythematosus and animal models, applied in the direction of medical raw materials derived from mammals, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve the problems of short duration, active immunity, specific defects, etc., and achieve the goal of onset Fast and efficient results

Active Publication Date: 2018-02-16
THE THIRD AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The murine systemic lupus erythematosus model induced by subcutaneous immunization with DNA derived from activated lymphocytes is currently a relatively common induced systemic lupus disease model, but it has defects in active immunity and specificity, and the duration is short, which is harmful to the system. There are still some limitations in the study of the disease mechanism of lupus erythematosus

Method used

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  • Construction method of systemic lupus erythematosus animal model and application thereof
  • Construction method of systemic lupus erythematosus animal model and application thereof
  • Construction method of systemic lupus erythematosus animal model and application thereof

Examples

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Embodiment 1

[0044] Example 1 An example of the construction method of the animal model of systemic lupus erythematosus and its application, wherein the schematic diagram of the construction method is as follows figure 1 As shown, the specific steps are as follows:

[0045] 1. Extract the DNA of lymphocytes in the state of apoptosis, the method is as follows:

[0046]1) The C57BL / 6 mice were killed by carbon dioxide asphyxiation, and the spleen was taken out aseptically, and placed in PBS on ice until all the mice were prepared; 2 Grind the spleen with sterile glass slides in a plate to make a single cell suspension;

[0047] 2) Collect the suspension in a 50ml centrifuge tube after passing through a sieve (removing granular connective tissue), centrifuge at 1500rpm at 4°C for 5 minutes, and discard the supernatant;

[0048] 3) Add 3ml of red blood cell lysate ACK, let stand at room temperature for 3 minutes, centrifuge at 1500rpm for 5 minutes, and discard the supernatant;

[0049] 4) ...

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Abstract

The invention discloses a method for constructing an animal model of systemic lupus erythematosus. According to the method, after dendritic cells of mice are coated in vitro with lymphocyte DNA in apoptosis state for 12 hours, the dendritic cells are tail-vein injected into mice body circulation to induce systemic lupus erythematosus in mice. Systemic lupus erythematosus disease in mice is evaluated by detecting the increase of autoantibody level in mice, hyperplasia and swelling of mice lymphoid tissues, and later changes in renal glomerular inflammation traits in mice. This method can help induce mice systemic lupus erythematosus within 2 weeks, and the induction model success rate reaches up to 100%. The method of the invention is a rapid, concise, efficient, immune cell and immunogen clear construction method of the systemic lupus erythematosus animal model, and can facilitate the exploration of the mechanism of systemic lupus erythematosus disease and the search for new and effective treatment methods.

Description

technical field [0001] The invention relates to a method for constructing an animal disease model, in particular to a method for constructing an animal model of systemic lupus erythematosus and its application. Background technique [0002] Systemic lupus erythematosus is a common autoimmune disease characterized by multi-system chronic inflammatory damage to organs and tissues. Its etiology and pathogenesis are not yet fully understood. Effects, causing immune homeostasis imbalance, regulation disorder, antigen-antibody complexes, complement complex deposition in blood vessels, causing immune response and thrombosis, eventually leading to local or systemic tissue and organ damage. [0003] Systemic lupus erythematosus cannot be cured temporarily. The current treatment methods are mainly cellular immunosuppressants and glucocorticoids. Although glucocorticoids and immunosuppressants can effectively alleviate the condition of systemic lupus erythematosus, patients are still p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/17A01K67/02A61K35/15
CPCA01K67/02A61K35/15A61K35/17A61K2300/00
Inventor 肖泽秀郑旭郑颂国黄峰
Owner THE THIRD AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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