A method of synthesizing beta-caryophyllene by microbial catalysis and a reconstituted cell capable of synthesizing the beta-caryophyllene are disclosed. Acetyl coenzyme A used in the method is obtained from simple initial materials such as glucose. The method includes: A) a step of constructing the reconstituted cell capable of synthesizing the beta-caryophyllene from the acetyl coenzyme A, wherein the reconstituted cell comprises gene segments comprising acetyl-CoA acyltransferase, 3-hydroxy-3-methylglutaryl coenzyme A synthetase, hydroxymethylglutaryl-CoA reductase, mevalonate kinase, phosphomevalonate kinase, diphosphomevalonate decarboxylase, isopentenyl diphosphate isomerase, geranyl pyrophosphate synthase, farnesyl pyrophosphate synthase, and beta-caryophyllene synthase; and B) a step of culturing the reconstituted cell in a culture medium containing glucose, inducing with a proper inducer, separating and purifying to obtain the beta-caryophyllene.