30results about How to "Guaranteed to successfully build" patented technology

The invention provides a network switching method, a mobile network system and a mobile management device; wherein the network switching method comprises: the mobile management equipment on the target side acquires the related anchor point information of the currently used user plane anchor point equipment of a home network or a visit network; the mobile management equipment on the target side selects the corresponding mobile anchor point equipment on the target side according to the related anchor point information of the currently used user plane anchor point equipment of a home network or a visit network and triggers the mobile anchor point equipment to construct connection with the user plane anchor point equipment. The technical proposal provided by the invention embodiment can ensure successful and rapid construction of user plane carrier on the target side.

The invention provides Saccharopolyspora pogona delta Clu13-MmsA and a construction method and application thereof. The Saccharopolyspora pogona delta Clu13-MmsA is preserved in the China Center for Type Culture Collection, and the preservation number of the strain is CCTCC NO: M2021825. The construction method of the Saccharopolyspora pogona delta Clu13-MmsA is easy and convenient to operate, the Saccharopolyspora pogona delta Clu13-MmsA is used for producing butenyl spinosad through fermentation, the yield of the butenyl spinosad is increased by 10.1 times compared with that of Saccharopolyspora pogona original bacteria, and meanwhile the yield of the butenyl spinosad is far higher than that of an S.pogona-MmsA strain.

The invention provides a method for supporting correct establishment of a local IP access (LIPA) service. User equipment (UE) first obtains a public land mobile network (PLMN) supporting the LIPA service and makes a request to establish the LIPA service in the PLMN supporting the LIPA service according to the PLMN supporting the LIPA service. The invention further provides two other methods for supporting correct establishment of the LIPA service, and the two methods are suitable for a network side device. The LIPA service can be established for the UE correctly by applying the method, the service requirements of a user are met to the greatest extent, and optimal network resource distribution is achieved.

The invention relates to an attenuated pseudorabies virus with a deletion gene, as a vaccine, a vaccine and a construction method, and particularly provides an attenuated pseudorabies virus (PRV), wherein all or part of a Us3 gene of a Us region is deleted. Experiment proves that the virus strain has 100% protein induction for attack of parent PRV virulent viruses on skin and hair animals of dogs,minks, foxes, racoon dogs and the like. The attenuated PRV virus strain can be used as a vaccine for safely and effectively preventing and controlling epidemic situation of pseudorabies (PR) of the skin and hair animals, and has great social value.

The invention discloses a method, device and system for establishing a PC5 link, relates to the technical field of communications, and aims at solving the problem in the prior art that a PC5 link between UEs cannot be better established. The method comprises that first UE sends a direct communication request message carrying an identifier of the first UE and used for requesting to establish a PC5link; and the first UE receives a direct communication receiving message sent by second UE, wherein the direct communication receiving message carries the identifier of the second UE, and is used forreceiving establishment of a PC5 link with the first UE. The scheme is specifically applied to a scene of establishing a PC5 link between UEs.

The invention discloses a prokaryotic expression vector for a recombinant protein of casein kinase I(alpha). The prokaryotic expression vector contains a nucleotide sequence as shown in SEQ ID No. 1,a restriction enzyme cutting site Nde I and a restriction enzyme cutting site BamH I. A construction method for the prokaryotic expression vector for the recombinant protein of the casein kinase I(alpha) comprises the following steps: designing primers used for amplifying CKI(alpha) according to the nucleotide sequences of mouse casein kinase I(alpha) included in the GenBank; acquiring the whole cDNAs of mouse cells; cloning a CKI(alpha) gene in the cDNAs by using the primers used for amplifying CKI(alpha); and connecting the cloned CKI(alpha) gene with a prokaryotic expression vector so as toobtain the recombinant prokaryotic expression vector. The prokaryotic expression vector for the recombinant protein of the casein kinase I(alpha) and the construction method thereof successively utilize a prokaryotic expression system to prepare recombinant CKI(alpha).

The invention discloses a construction method of a progressive cerebral arterial thrombosis model. Progressive cerebral arterial thrombosis is a special type of stroke, and has the characteristics of continuous exacerbation of neurological dysfunction, poor prognosis, lack of effective drugs, and very difficult clinical treatment. Due to the lack of animal models capable of accurately simulating the pathological process of progressive stroke, the research on the pathological mechanism of the disease and the research and development of therapeutic drugs are greatly limited. According to the method, related experimental parameters of a photochemical embolism method are improved, so that the cerebral ischemia injury degree of animals accords with the pathological characteristics of progressive stroke, and the progressive cerebral ischemic stroke model is successfully constructed. The method has the main beneficial effects that a new modeling method of the mouse progressive cerebral arterial thrombosis model is provided, and the blank of progressive cerebral arterial thrombosis animal models in academic circles is filled. Meanwhile, the operation is simple, convenient and feasible, the animal wound is small, the repeatability is good, the process is close to the pathogenesis process of human thrombotic cerebral apoplexy, and the method has important application value in the research of the pathological mechanism of the disease and the research and development of new drugs and new targets.

The invention relates to the field of cell gene engineering, and particularly discloses a construction method and application of an ARHGAP11B gene deleted iPSC (induced pluripotent stem cell) cell line. According to the present invention, the targeting site of the specific sgRNA for targeted knockout of the human ARHGAP11B gene on the human ARHGAP11B gene is located on the No.6 exon of the human ARHGAP11B gene; the specific sgRNA provided by the invention can efficiently target the human ARHGAP11B gene, and a method for knocking out the ARHGAP11B gene in an iPSC cell line is constructed based on a CRISPR/Cas9 system, and the method is simple and convenient to operate and more thorough in knockout effect.

The invention relates to a saccharopolyspora tuberosa engineering strain with doubled pII gene and a construction method and application thereof, the strain is saccharopolyspora tuberosa S.pogona-pII and is preserved in China Center for Type Culture Collection on June 9, 2021, and the preservation number of the strain is CCTCC M 2021706 PII. The construction method comprises the following steps: constructing a pOJ260-PkasO-pII recombinant plasmid on the basis of wild type saccharopolyspora tuberosa, and introducing the recombinant plasmid into the saccharopolyspora tuberosa by utilizing a conjugational transfer method. When the S.pogona-pII is used for synthesizing the butenyl spinosad, the yield of the S.pogona-pII is obviously higher than that of a wild type strain and is 245% of that of the butenyl spinosad of the wild type strain.