Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Construction method of progressive cerebral arterial thrombosis model

A technology of ischemic stroke and construction method, which is applied in animal husbandry and other fields, can solve the problems of inability to accurately reflect the characteristics of pathological changes in progressive stroke, inability to directly apply research, secondary deterioration, etc.

Pending Publication Date: 2021-09-14
THE FIRST AFFILIATED HOSPITAL ZHEJIANG UNIV COLLEGE OF MEDICINE
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The classic thread embolism model, photochemical embolism model and rat cerebral thromboembolism model mainly simulate the routine pathogenesis of acute ischemic brain injury, and cannot accurately reflect the pathological changes of progressive stroke—one week after cerebral infarction The neurological function of left and right patients will show signs of secondary deterioration, so it cannot be directly applied to the study of progressive stroke. It is urgent to construct a new animal model for progressive stroke to promote the research on the pathological mechanism of this type of disease and related therapeutic drugs R & D process

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Construction method of progressive cerebral arterial thrombosis model
  • Construction method of progressive cerebral arterial thrombosis model
  • Construction method of progressive cerebral arterial thrombosis model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Construction of a mouse model of progressive ischemic stroke induced by photochemical embolization

[0031] (1) Choose C57BL / 6J wild-type male mice aged 10-12 weeks and weighing 23-25 ​​g, and maintain anesthesia with sevoflurane gas;

[0032] (2) Fix the head of the mouse on the stereotaxic instrument, remove the head hair and disinfect the surface, cut the skin through the median incision, expose the bregma, and fix an optical fiber with a diameter of 2.0mm close to the top of the skull;

[0033] (3) The light emitting diameter of the cold light source is 2.0mm, and the light source is vertically attached to the top of the skull, and the center of the light source is shifted to the right by 1.5mm compared with the brine;

[0034] (4) Rose Bengal was injected intraperitoneally at a dose of 100 mg / kg, and then irradiated with a cold light source for 15 minutes after 5 minutes, and the intensity of the cold light source was 16000 lux;

[0035] (5) After the i...

Embodiment 2

[0040] Example 2: Progression of neurological deficits within 14 days after progressive ischemic stroke mice were modeled

[0041] Mouse Stagger Test:

[0042] The mice were placed on a grid with a side length of 40 cm and an inner 1 cm square, the grid was made of iron wire with a diameter of 1 mm, and the height from the ground was 30 cm. A camera is set at the bottom of the grid to record the free movement of the mouse on the grid. When a normal mouse moves freely, both front and rear feet step on the wire. Due to the influence of cerebral ischemia, the front and rear feet on the opposite side of the ischemic brain step on the empty grid. Every missed step is counted as a wrong step. Take pictures of the total number of 100 steps of the mouse's free activities, record the number of wrong steps and calculate the wrong step rate ( figure 2 A).

[0043] Cylinder test:

[0044] Put the mice in a transparent cylinder with a diameter of 10 cm and a height of 15 cm, and place...

Embodiment 3

[0047] Example 3: Progressive ischemic stroke in mice without increased neuronal loss

[0048] Immunohistofluorescence staining:

[0049] First, slides were permeabilized with pre-cooled methanol at -20°C for 15 min. Rinse twice with 1×PBS at room temperature, 5 min each time. Block with blocking solution (PBS+2.5% donkey serum / goat serum, 0.2% Triton X-100) for 1 h at room temperature. Dilute the specific primary antibody (1:200) in blocking solution and incubate overnight at 4°C. On the next day, rewarm on a shaker for half an hour, rinse 3 times with 1×PBS for 10 minutes each time, add the corresponding secondary antibody (1:400) in a dark environment and incubate at room temperature for 1-2 hours. Then, rinse 3 times with 1×PBS, 10min each time. Mount the slides with DAPI-containing mounting solution, cover the coverslip without generating any air bubbles, and seal the edges with transparent nail polish. Store at 4°C in the dark, and observe with an inverted confocal ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Diameteraaaaaaaaaa
Thicknessaaaaaaaaaa
Login to View More

Abstract

The invention discloses a construction method of a progressive cerebral arterial thrombosis model. Progressive cerebral arterial thrombosis is a special type of stroke, and has the characteristics of continuous exacerbation of neurological dysfunction, poor prognosis, lack of effective drugs, and very difficult clinical treatment. Due to the lack of animal models capable of accurately simulating the pathological process of progressive stroke, the research on the pathological mechanism of the disease and the research and development of therapeutic drugs are greatly limited. According to the method, related experimental parameters of a photochemical embolism method are improved, so that the cerebral ischemia injury degree of animals accords with the pathological characteristics of progressive stroke, and the progressive cerebral ischemic stroke model is successfully constructed. The method has the main beneficial effects that a new modeling method of the mouse progressive cerebral arterial thrombosis model is provided, and the blank of progressive cerebral arterial thrombosis animal models in academic circles is filled. Meanwhile, the operation is simple, convenient and feasible, the animal wound is small, the repeatability is good, the process is close to the pathogenesis process of human thrombotic cerebral apoplexy, and the method has important application value in the research of the pathological mechanism of the disease and the research and development of new drugs and new targets.

Description

[0001] (1) Technical field [0002] The invention relates to a method for constructing a progressive ischemic stroke model. [0003] (2) Background technology [0004] Ischemic stroke is a serious neurological disease, which has become the main cause of death and disability in my country. Progressive ischemic stroke (PIS) is a special type of ischemic stroke, which means that despite active clinical intervention, the primary pathological process leading to cerebral ischemia still continues to progress, with onset of several days to a week In China, a type of cerebral infarction in which neurological deficits gradually progress or become progressively aggravated accounts for about 30% of ischemic strokes. Compared with other stroke types, patients with progressive stroke have a higher disability and mortality rate, worse prognosis, and more severe neurological deficits. Once it occurs, only symptomatic treatment is the mainstay, which is a type of stroke that is difficult to co...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01K67/027
CPCA01K67/027A01K2207/30A01K2207/35A01K2207/20A01K2267/0375
Inventor 吴佳莹张翔南胡薇薇陈忠洪东升潘玲唐卫东
Owner THE FIRST AFFILIATED HOSPITAL ZHEJIANG UNIV COLLEGE OF MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com