The invention belongs to the technical field of zoocytologic experiments and particularly relates to a method for preparing a
chromosome of
procambarus clarkii. The method comprises the steps: separately carrying out injection treatment on
procambarus clarkii bodies by using phytohemagglutinin PHA and
colchicine, then, taking antennal glands of the
procambarus clarkii, rinsing the antennal glands in normal
saline, then, shearing tissue into muddy tissue, and carrying out a
centrifugation method, so as to obtain a
cell suspension; separately carrying out hypotonic treatment and pre-immobilization by using a 0.75% KCl solution and an immobilization solution-1; then, carrying out immobilization
on cells separately by using the immobilization solution-1 and an immobilization solution-2 which are in the ratio of 3:1; obliquely placing a precooled
glass slide, sucking a proper volume of
cell suspension by a
pipette, vertically spraying the
cell suspension onto the
glass slide in a rapid and powerful manner, rapidly baking the
glass slide over
flame of an
alcohol lamp for 2 to 3 seconds, and airing the glass slide in;
staining the glass slide accompanied by the
chromosome with a Giemsa
staining solution; carrying out microscopical observation and photographing. The method has the advantages of relatively good
chromosome dispersion, discernible form and countable chromosome split phase.