The invention relates to the technical field of
crop breeding, in particular to a
pollination method and device for breaking the self-incompatibility of
isatis tinctoria, and the method comprises the following steps: selecting plants, trimming, installing an
insect prevention cover, spraying, pollinating, removing an
insect prevention device and harvesting. The invention provides the
pollination method for breaking the self-incompatibility of the
isatis tinctoria, the method breaks the self-incompatibility of the
isatis tinctoria, realizes self-
pollination, improves the breeding effect of the
isatis tinctoria, has the characteristics of low production cost, good pollination effect and easiness in operation, can greatly improve the self-pollination efficiency of the
isatis tinctoria and obtain a
selfing line with higher purity, and can be applied to the field of breeding of the
isatis tinctoria. The
insect pollination hybridization can be well prevented, the isatis tinctoria breeding time can be saved, and the method has a very high application value in the field of isatis tinctoria breeding.