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Method for large scale cultivating common wheat near isogenic line

A near-isogenic system and a large number of technologies, applied in the fields of plant genetic improvement, botanical equipment and methods, and applications, can solve problems such as increased workload, difficulty in statistical analysis, and impact on the reliability of research results, saving manpower and Material strength, the effect of improving accuracy

Inactive Publication Date: 2006-11-08
NORTHWEST A & F UNIV
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Problems solved by technology

However, the classic backcrossing method to breed near-isogenic lines often only breeds a pair of near-isogenic lines in a single trait combination, which increases the workload for a large number of near-isogenic lines; otherwise, it will bring problems for statistical analysis. Difficulty, affecting the reliability of the research results

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  • Method for large scale cultivating common wheat near isogenic line
  • Method for large scale cultivating common wheat near isogenic line

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Embodiment 1

[0021] Such as figure 1 As shown, by crossing the parent Xiaoyan 6 (containing the gene encoding 14+15) and the parent Pavon (containing the gene encoding 17+18), using the law that self-pollinated crops tend to be homozygous from generation to generation, in In the process of gene homozygosity, biotechnology is used to track and detect the target subunit traits in each generation, that is, starting from the F2 generation, the pedigree selection is carried out the same as the conventional pedigree selection, and the target trait subunit heterozygous strains (both coding 14+ 15 is coded 17+18) and numbered (such as: Xiaopan-1, -2...-16), and pedigree selection and numbering will be carried out on these strains in the coming year (Xiaopan-1-1, -1-2, -2 ...-16-2), in turn, until the other agronomic traits of the strain are stable (about 7-8 generations), self-select two homozygous types, one encoding 14+15 subunit type, one encoding The 17+18 subunit type comes from two differen...

Embodiment 2

[0023] Breeding of near-isogenic lines with stripe rust resistance gene. Such as figure 2 As shown, the material 9434 containing the dominant disease-resistant gene was crossed with the ice-sensitive material 626 (9434×626), F2 was inoculated with stripe rust in the field, and a single disease-resistant plant (at least 4 plants) was selected, and F3 was planted. Select disease-resistant single plants from the strains separated by resistance, and plant the strains in the next year, eliminate stable or fully susceptible strains (one target gene is lost), and then randomly select among the strains isolated from resistance, and proceed sequentially By the 7th to 8th generation, the genetic background was stable and the target resistance was heterozygous. In the first generation of selfing, the disease-resistant and susceptible plants are selected to form a pair of near-isogenic lines, which form a near-isogenic group with the near-isogenic lines of other offspring types of resis...

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Abstract

The present invention discloses a method of large scale cultivating common wheat near isogenic line. Based on the rule that the self pollination crop has gradual selfing gene isozygoty, the target subunit character of each generation during the gene isozygoty process is tracked and detected by means of biological technology. Through 7-8 generations, the agronomic character of the strain become stable, and two isozygoty types as the paired near isogenic lines of target gene are selfing selected. By means of the said method, several pairs of near isogenic lines may be cultivated inside the same combination, and this result in saving in manpower and material, is favorable to target subunit character analysis and has raised accuracy of statistic and analysis results.

Description

technical field [0001] The invention belongs to the field of agricultural science and relates to a method for mass-breeding near-isogenic lines of common wheat. Background technique [0002] Due to the characteristic of individual target quality trait gene differences, near-isogenic lines provide convenience and reliability for research on the role and effect of target quality trait genes. Therefore, in agricultural scientific research, wheat breeders are keen on the cultivation and research utilization of near-isogenic lines. At present, the commonly used method of cultivating classical near isogenic lines is selective continuous backcrossing, using biochemical markers to transfer a certain trait gene or protein subunit pair to a main wheat variety, and comparing different trait genes or different protein subunit pairs in the same variety. Effects of subunit differences on physiological, biochemical or quality traits. However, the classic backcro...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
Inventor 吉万全张宏任志龙王长有王秋英王亚娟蔡东明
Owner NORTHWEST A & F UNIV
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