Seed production method for producing high-purity hybrid rape seeds by using double-pollination control system

Abstract

The invention discloses a seed production method for producing high-purity hybrid rape seeds by using a double-pollination control system, which combines a self-incompatible line with a chemical male killing technique, uses the self-incompatible line as a female parent of the hybrid seeds to perform inter-planting with a male parent restoration line. A chemical male gametocide is sprayed from the time before blossom to the time of first blossom of the female parent, so the probability of self-pollination of the female parent can be significantly reduced. When the female parent is hybridized with the male parent restoration line, the high-purity hybrid rape seeds can be produced.

Description

technical field [0001] The invention belongs to the field of obtaining new plants, and relates to the utilization of rapeseed heterosis, in particular to a method for producing high-purity rapeseed hybrids with a dual pollination control system. Capable of producing high-purity hybrids. Background technique [0002] The utilization of crop heterosis must depend on a stable and reliable pollination control system. In rapeseed, self-incompatibility and chemical male killing are important pollination control systems. In particular, the male parents required by the two methods to prepare hybrid combinations are basically not restricted by genotype, and the selection range is very wide. Two-line hybrids can be directly produced, which is of positive significance for expanding the range of heterosis utilization in rapeseed. [0003] In Brassica napus, Brassica oleracea, Brassica napus x Brassica napus hybrid offspring, Brassica napus x Brassica napus, self-incompatibility phenom...

AI Technical Summary

Problems solved by technology

In particular, self-incompatibility lines are not stable enough in special climatic conditions such as high temperature environments;

[0005] (2) Self-incompatibility lines lack obvious morphological characteristics, and it is difficult to identify and remove impurities (Wang Hanzhong et al., patent application number 200410060822.5)

[0006] Especially the first factor causes the risk of self-incompatible line seed production to be relatively large, and the hybrid rate is sometimes less than 70% (Zhang Ruimao et al., Breeding of Brassica napus self-incompatible line and its hybrid seed production and temperature Research on Relationship, Guizhou Agricultural Science, 1993, Issue 6: 1-5)

[0008] In short, the advantages and disadvantages of chemical male killing of self-incompatibility lines are very prominent. The common advantages are that the selection of male parents (or restorer lines) is basically not restricted by genotype, the degree of freedom in the preparation of hybrid combinations is large, and the hybrid vigor potential ratio of the two lines is relatively large. The cytoplasmic sterile three-line system is larger; the disadvantages of self-incompatible lines are that self-fertilization can be strong and unstable at high temperature, and the disadvantages of chemical male killing are strong technical dependence and great environmental impact

These shortcomings severely limit the unique advantages of the two systems in the production of two-line hybrid rapeseed