Cucumber downy mildew resistance main effect QTL linkage molecule labeling method and applying method

A technology for cucumber downy mildew and molecular markers, which is applied in the directions of biochemical equipment and methods, microbial determination/inspection, etc., and can solve the problem of long breeding cycle, difficulties in downy mildew-resistant materials, and difficulty in identifying resistant materials. Control and other issues to reduce workload, improve selection accuracy and breeding efficiency

Inactive Publication Date: 2008-08-13
SHANGHAI JIAO TONG UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

In conventional breeding practice, it is very difficult to select materials resistant to downy mildew: on the one hand, the breeding cycle is long; on the other hand, the occurrence of downy mildew is affected by many fac

Method used

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  • Cucumber downy mildew resistance main effect QTL linkage molecule labeling method and applying method
  • Cucumber downy mildew resistance main effect QTL linkage molecule labeling method and applying method
  • Cucumber downy mildew resistance main effect QTL linkage molecule labeling method and applying method

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Experimental program
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Effect test

Embodiment 1

[0028] Molecular marker method of closely linked major QTL for cucumber downy mildew resistance

[0029] (1) Hybridization of S94 with cucumber downy mildew susceptible cultivar S06 to produce F 1 mongrel.

[0030] (2)F 1 F 2 , F 2 The 252 single plants of the 252 plants were randomly selected for planting a seed, and propagated to F 7 generation, resulting in 224 families.

[0031] (3) Select SRAP, SSR, SCAR and STS primer combinations with amplified polymorphisms between the two parents, and use existing methods for 224 families (Wang Gang, Chinese Science C Life Science 2004, 34 (6): 510 ~516; Sakata Y, Theor Appl Genet (Applied Genetic Theory), 2006, 112: 243~250) for analysis, isolate the DNA of cucumber leaves of each recombinant inbred line, and use related sequence amplified polymorphism (SRAP), Microsatellite (SSR), sequence-specific amplification region (SCAR) and sequence tag site (STS) molecular marker primers carry out PCR amplification, and the amplified pr...

Embodiment 2

[0039] Example 2: Verification of molecular markers linked to the main QTL for downy mildew resistance of S94 in high-generation genetic populations:

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Abstract

The present invention relates to a molecular marker method linked with major QTL for cucumber downy mildew resistance and an application method, belonging to the field of biological technology. The molecular marker method: getting F2 filial generation by self-pollination of hybrid F1; acquiring F7 generation recombinant inbred lines by filial generation single seed descent method; describing the gene type of each strain of the recombinant inbred lines; constructing genetic linkage map for molecular marker; testing the downy mildew resistance of each strain of the recombinant inbred lines; and composite interval mapping for interval possible located in QTL. The application method: hybridizing S94 and derive verities thereof with other cucumbers and multiplying to F2 generation; separating genome DNA of single cucumber plant, and testing whether each cucumber plant has molecular marker method linked with major QTL for cucumber downy mildew resistance. The invention develops an efficient, rapid breeding technology, selects breeding variety with conventional downy mildew, reduces workload of breeder, and improves precise of selection and breeding efficiency.

Description

technical field [0001] The invention relates to a marker method in the field of molecular biology technology, in particular to a molecular marker method and an application method for the main effect QTL linkage of cucumber downy mildew resistance. Background technique [0002] Downy mildew (downy mildew) is one of the important diseases of cucumber (Cucumis sativus L.) producing areas all over the world. In my country, downy mildew is listed as the three major diseases of cucumber along with downy mildew and Fusarium wilt. Cucumber downy mildew is caused by Pseudoperonospora cubensis (Berk. & Curt.) Rostov.], which belongs to the subphylum Flagellate, Peronosporales, and the genus Pseudoperonospora. It mainly harms the leaves of cucumbers, and can be diseased at the seedling stage and the adult plant stage. Under suitable conditions, its disease develops rapidly, and it will spread to the whole field in a few days, causing the leaves to turn yellow and die, and the yield wi...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 蔡润刘龙洲白智龙潘俊松何欢乐
Owner SHANGHAI JIAO TONG UNIV
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