12 results about "Genetic linkage map" patented technology

The invention discloses a positioning method of trachinotus ovatus growth trait related QTL (quantitative trait loci) based on 2b-RAD technology and application, the method uses 2b-RAD technology to perform sequencing on 300 trachinotus ovatus full-sib F1 generation and male and female parent individuals and develop SNP (single nucleotide polymorphism) markers, constructs a trachinotus ovatus high-density genetic linkage map, performs linkage positioning analysis in combination with 8 growth phenotype data, and finds that the trachinotus ovatus growth trait related QTL is found. 85 stable QTLs associated with the growth traits are screened out, and the stable QTLs contain 763 SNP sites. The invention also discloses an application of the method in a genetic map of trachinotus ovatus growth character positioning or molecular marker-assisted breeding of trachinotus ovatus, and the SNP molecular markers of trachinotus ovatus can be applied in genetic map construction and growth character positioning. Particularly, the method has a good application prospect in trachinotus ovatus growth trait molecular marker assisted breeding.

The present application relates to the technical field of molecular marker development and molecular detection, discloses development of dwarf tomato InDel molecular marker primer, provides a group of dwarf tomato InDel molecular markers, the dwarf tomato InDel molecular marker includes 79 InDel markers shown in the following table, and the dwarf tomato InDel molecular marker is determined with reference to genome SL5.0.Using the dwarf tomato InDel molecular marker can provide technical support for carrying out target DNA sample rapid molecular detection in dwarf tomato;Since the set of markers is distributed on multiple chromosomes of the dwarf tomato genome, can be selected and used according to actual needs, the genomic DNA of the candidate material can be detected at any stage, has the advantages of flexible use, simple operation, high detection efficiency, less limiting factors, stable and accurate detection results, provides a simple, flexible and efficient way for genetic linkage map construction, genetic diversity analysis, resource identification, hybrid / purity identification and the like in the dwarf tomato.

The invention belongs to the technical field of biology, and discloses a molecular marker ClChr022479391 related to a watermelon fruit shape and application of the molecular marker ClChr022479391. According to the present invention, the watermelon fruit shape is determined, the stable and reliable molecular marker ClChr022479391 closely linked to the watermelon fruit shape is obtained by constructing the high-density genetic linkage map, the molecular marker is the 2479391 site on the second dye of the watermelon reference genome 97103 V1, and the marker for SNP detection is developed based on the site. The invention also provides a method for quickly identifying the shape of the watermelon fruit by using the primer group of the molecular marker, and the method is simple and quick, accurate in identification result and good in popularization and application prospect.

The invention belongs to the technical field of biology, and discloses a molecular marker ClChr085111232 related to the weight of watermelon fruits and application of the molecular marker ClChr085111232. According to the molecular marker ClChr085111232, the fruit weight of watermelons in the mature period is measured, the molecular marker ClChr085111232 which is closely linked with the fruit weight of the watermelons and is stable and reliable is obtained by constructing a high-density genetic linkage map, the molecular marker is located at the 5111232 site on the No.8 dye of a watermelon reference genome 97103V1, and the marker for SNP detection is developed on the basis of the site. The invention also provides a method for quickly identifying the weight of the watermelon fruit by using the primer group of the molecular marker, and the method is simple and quick, accurate in identification result and good in popularization and application prospect.

This invention discloses a primer set for SSR molecular markers in *Pterocarya stenoptera* and its applications. By analyzing *Pterocarya stenoptera* germplasm samples from different regions, this invention obtained SSR molecular markers that can be used for the identification of the germplasm source (property) of *Pterocarya stenoptera*. Based on this, this invention also provides a primer set for SSR molecular markers in *Pterocarya stenoptera*. This primer set can not only be used for the identification of the propagation of *Pterocarya stenoptera*, but also for the analysis of genetic diversity and the construction of genetic linkage maps, exhibiting advantages such as high polymorphism, good repeatability, marker stability, and clear and easily interpretable banding patterns. Furthermore, this invention also provides a detection kit for SSR molecular markers in *Pterocarya stenoptera*, which can be used for the identification of the propagation of *Pterocarya stenoptera*, the construction of DNA fingerprint maps, and the evaluation of genetic diversity and phylogenetic studies, thus contributing to the molecular-assisted breeding of *Pterocarya stenoptera*.

The application belongs to the technical field of biology, and discloses a molecular marker Cl_Chr02_2479391 related to watermelon fruit shape and application thereof. The application determines the watermelon fruit shape, obtains a molecular marker Cl_Chr02_2479391 which is closely linked to the watermelon fruit shape and stable and reliable by constructing a high-density genetic linkage map, the molecular marker is located at the 2nd chromosome of the watermelon reference genome 97103 V1 and the 2479391th position, and a marker for SNP detection is developed based on the position. The application further provides a method for rapidly identifying the watermelon fruit shape by using the primer set of the molecular marker, the method is simple and fast, the identification result is accurate, and the method has a good popularization and application prospect.

The application discloses an InDel molecular marker for identifying pepper purple fruits, primers and application thereof. The application locates a linkage candidate interval related to the purple fruits by constructing a genetic linkage map of different pepper fruit colors, screens candidate genes and InDel markers in the interval, and develops the InDel molecular marker for identifying pepper purple fruits and the primers, so that the pepper purple fruits can be accurately and quickly distinguished and identified. Meanwhile, the application also provides a method for identifying pepper purple fruits or typing of pepper purple fruit plants, which can more accurately type pepper parents and offspring, identify F1 generation and parental purple traits, solves the problems that only the purple traits can be identified in the prior art and the F1 generation traits of the pepper purple fruits are not accurately typed, shortens a screening period of pepper purple fruit germplasm resources, and provides technical support for identification of the pepper purple fruits and offspring.

This invention relates to the field of genetic engineering technology, and more particularly to a molecular marker for detecting wheat leaf rust resistance genes LrN4B or LrN3B and its application. The molecular marker LrN4B-1 is located in the 578-585Mb region of chromosome 4BL, with a polymorphism of C / G. The molecular marker LrN3B-1 is located in the 4-8Mb region of chromosome 3BS, with a polymorphism of T / C. This invention obtains molecular markers linked to wheat leaf rust resistance genes LrN4B and LrN3B through the construction of genetic linkage maps and the screening and localization of molecular markers. These two molecular markers can be used to distinguish the LrN4B+LrN3B disease-resistant genotype, screen for wheat lines containing these two disease-resistant genes, accelerate the process of wheat disease-resistant breeding, and are of great significance in the field of breeding leaf rust-resistant wheat.

The present application belongs to the field of genetic engineering and molecular breeding, and particularly relates to a wheat callus induction rate QTL positioning and closely linked molecular marker and application. The present application uses the material Jimai 262 with high callus induction rate and Yanong 24 with excellent agronomic performance and relatively low callus induction rate as parents to construct a DH line, positions the QTL about the high callus induction rate of wheat by constructing a high-throughput SNP marker genetic linkage map. Meanwhile, the related molecular marker is developed to provide accurate genetic markers for anther culture breeding. The present application has important significance for improving the breeding efficiency and accelerating the breeding process.

This invention belongs to the field of biotechnology and discloses a molecular marker Cl_Chr08_5111232 related to watermelon fruit weight and its applications. This invention measures the weight of watermelon fruits at maturity and, by constructing a high-density genetic linkage map, obtains a stable and reliable molecular marker Cl_Chr08_5111232 that is closely linked to watermelon fruit weight. This molecular marker is located at position 5111232 on chromosome 8 of the watermelon reference genome 97103V1, and a marker for SNP detection is developed based on this site. This invention also provides a method for rapidly identifying watermelon fruit weight using primer sets based on the aforementioned molecular marker. This method is simple, fast, and accurate, and has good prospects for widespread application.

The invention discloses a molecular marker closely linked with eggplant peel stripes, a KASP primer and application of the molecular marker and the KASP primer. According to the method, WT and BW2-2 are taken as parents, an F2 hybrid population is constructed, a genetic linkage map is constructed through statistical analysis of population pericarp stripe traits and combination of InDel sites obtained through genome re-sequencing, and QTL positioning analysis of eggplant pericarp stripes is carried out. And finally, an InDel molecular marker significantly related to the eggplant peel stripes is screened, and the marker is located at the 4043249 bp site of the chromosome 4. Three KASP primers designed based on the site can be directly used for early identification and screening of corresponding genotypes of eggplant peel stripes, and rapid and accurate identification of target phenotypes is realized. The marker provides reliable technical support for molecular-assisted selection of eggplant peel stripe traits, the backcross offspring breeding time can be remarkably shortened, the breeding workload is reduced, and the breeding efficiency is improved.

The present application relates to the field of oilseed rape molecular breeding and biotechnology, and particularly relates to application of QTL site of Brassica napus in construction of SNP genetic linkage map for positioning nitrogen efficient absorption related traits. The present application provides application of QTL site of Brassica napus in construction of SNP genetic linkage map for positioning nitrogen efficient absorption related traits, which obtains a large number of SNP markers with gene sequences by determining phenotype data of nitrogen efficient absorption related traits of Brassica napus and high-throughput genotype data, constructs high-density molecular genetic linkage map of Brassica napus by using these marker sites, and positions nitrogen efficient absorption related traits of Brassica napus, obtains markers significantly associated with traits, mines QTL sites associated with nitrogen efficient absorption related traits, and the positioning site is good in stability and wide in application range, and provides basic information for molecular marker assisted breeding of nitrogen efficient absorption of Brassica napus.