76 results about "Chinese pistachio" patented technology

The invention discloses a method for removing endophytes from pistacia chinensis bunge seeds and quickly germinating the pistacia chinensis bunge seeds. The method mainly comprises the following steps of: sterilizing the pistacia chinensis bunge seeds, of which the shells are removed by using ethanol and sodium hypochlorite; soaking the seeds in water to perform ultrasonic processing; and flushing the seeds, and then inoculating the seeds to a seed germination culture medium to obtain aseptic robust seedlings. By adoption of the method, the difficulty that the germination rate of the seeds is influenced by pollution caused by inexhaustive sterilization and long sterilization time is solved, and more importantly, the germination rate of the seeds is increased, the seedlings grow robustly, and a basis is provided for tissue culture and genetic transformation of pistacia chinensis bunge.

The invention discloses a breeding method of a new variety red leaf Pistacia chinensis Bunge. The method includes: drying and refrigerating collected seeds, and soaking the seeds in an auxin solution to germinate the seeds; employing a bactericide to perform sterilization and disinfection on the germinated seeds and seedbed, and at the same time conducting sterilization and disinfection on seedlings. According to the invention, optimal seed sources and collected seeds are dried and then preserved at low temperature, the auxin solution is employed to soak and germinate the seeds, so that the seed germination rate is increased from 1-3% to 10-15%. The bactericide is adopted for sterilization and disinfection of the germinated seeds and seedbed, and sterilization and disinfection are simultaneously carried out on seedlings, so that the seedling emergency rate of the seedlings is enhanced from 20-30% to 80-90%. Both the naphthylacetic acid and gibberellin used by the method provided by the invention have the functions of breaking plant seed dormancy and promoting seed germination. The method is simple and is convenient to operate, and improves the germination rate.

The invention provides a method for quickly propagating pistacia chinensis bunge by using stem sections, which comprises: cutting off stem sections from pistacia chinensis bunge plants, disinfecting and inoculating onto a bud-inducing culture medium; transferring induced buds into a rooting culture medium to obtain whole plants; cutting induced tender shoots a plurality of stem sections; and repeatedly culturing in the same way to realize quick propagation. The offsprings of plants cultured by the method have few variations, and thus, a support is provided for the quick development of pistacia chinensis bunge ecological energy forests.

The invention discloses a natural multi-functional foliage fertilizer. The foliage fertilizer comprises the following raw materials in percentage by weight: 10-30% of Pistacia chinensis extraction solution, 5-10% of amino acid nutrient solution, 1-3% of trace elements, 2-4% of attapulgite, 1-3% of diatomaceous earth, and the balance of water. The invention also provides a preparation method of the foliage fertilizer, the method comprises the steps of taking leaves, buds, twigs and fruit peels of Pistacia chinensis, drying and crushing to fine powder; adding fine powder into the extraction solution at a ratio of fine powder to extraction solution being 1g/(5-40) ml and soaking; after soaking, heating, stirring and extracting, filtering the primary extraction solution and collecting the filtrate; centrifuging the filtrate, collecting the supernatant as the Pistacia chinensis extract solution; taking the raw materials in percentage by weight, mixing the raw material, heating and stirring until the raw materials are fully dissolved. The natural multi-functional foliage fertilizer has the advantages that the foliage fertilizer is natural, green, environmentally friendly and efficient, the yield-increasing effect is obvious, no residues of pesticides and fertilizers occur, the natural multi-functional foliage fertilizer can prevent pests and diseases of plants and is especially applicable to organic agriculture.

The invention is aimed at providing pistacia chinensis bunge microsatellite loci, a primer and application, and provides the twelve pistacia chinensis bunge microsatellite loci, and the primer for designing the microsatellite loci and detecting the population genetic variation of pistacia chinensis bunge. The twelve microsatellite loci are obtained from a pistacia chinensis bunge genome through a magnetic bead enrichment method and a fluorescent label sequencing technology, the specific primer is designed according to flanking sequences of two ends of a microsatellite, the amplification result has high polymorphism and stability and can be used for the fields of population genetic diversity detection, genetic relationship identification, establishment of a genetic linkage map, molecular mark assisted breeding and the like of pistacia chinensis bunge.

The invention discloses a preparation method of a food-medicine fungus fermentation product rich in folate. The preparation method comprises inoculating activated phellinus igniarius and lucid ganoderma strains in a liquid seed medium added with a pistacia chinensis bunge extract, culturing to obtain liquid strains of phellinus igniarius and lucid ganoderma; inoculating the liquid phellinus igniarius strain into a solid medium added with carrot and barley, culturing, drying and crushing a cultured product to obtain a solid culture I; then inoculating the liquid lucid ganoderma strains into a liquid fermentation medium added with the solid culture I and cabbage zymolyte, culturing for a certain while, then inoculating a lactobacillus strain, and culturing to obtain a fermentation product. According to the characteristics of fruits and vegetables rich in folate, like carrots and cabbages, and the fermentation characteristics of the food-medicine phellinus igniarius and lucid ganoderma, two-stage fermentation of a food-medicine fungus fermentation process and then a lactobacillus fermentation process is adopted, so that the content of the folate in the final fermentation product is increased, and the preparation method is a production method having an extensive industrial prospect.

The invention discloses a tissue culture and rapid propagation process of Chinese Pistache. The tissue culture and rapid propagation process of Chinese Pistache includes the steps of A, collecting ripe seeds of Chinese Pistache, and removing episperm by sulfuric acid digestion; sterilizing; inoculating the seeds to MS (Murashige and Skoog) minimal medium in which no hormone is added; B, cutting aseptic seedlings into stem segments with buds, removing part of leaves, inoculating to rooting medium or inoculating to enrichment medium prior to transferring to the rooting medium; and C, exercisingand transplanting the tissue culture seedlings. The tissue culture and rapid propagation process of Chinese Pistache has the advantages that the tissue culture method is modified, a medium formula isoptimized, and aseptic seedlings can be obtained easily and can be propagated rapidly and grow healthy and vigorous; mass high-quality test-tube seedlings can be obtained rapidly from the Chinese Pistache which is difficult or slow to propagate under natural conditions and can be applied to culture and production, and variation rate of offspring of the Chinese Pistache obtained by the process is low.

The invention discloses a pistacia chinensis bunge cutting propagation method under the condition of high-concentration CO2, which comprises the following steps: 1) dipping selected semi-ligneous pistacia chinensis bunge cuttings in plant growth regulator solution; and 2) cutting the treated cuttings in matrix, and then putting the matrix in an incubator with the air CO2 concentration of 750+/-50mu mol.mol<-1>-3000+/-50mu mol.mol<-1> for culture. In the pistacia chinensis bunge cutting propagation method, firstly the pistacia chinensis bunge semi-ligneous cuttings are dipped in a plant growth regulator, and then specific matrix, CO2 concentration, illumination intensity, air humidity, temperature and other culture conditions are selected for cutting propagation so as to greatly improve the cutting propagation survival rate and seedling quality of pistacia chinensis bunge.

The invention relates to the technical field of transplantation of pistacia chinensis, and particularly relates to a soil ecological remediation method suitable for transplantation of the pistacia chinensis and a method for transplanting the pistacia chinensis. The method comprises the following steps: sequentially distributing a composted straw composting layer, a livestock manure layer, a lime layer and a leguminous green manure plant planting layer at transplanted soil from bottom to top, and planting leguminous green manure plants on the leguminous green manure plant planting layer, wherein different layers are separated through thin soil; after the leguminous green manure plants are mature, pulling the leguminous green manure plants out, burying the leguminous green manure plants locally so as to form a leguminous green manure rotten material layer, and paving a compound bacteria agent soil layer on the leguminous green manure rotten material layer, wherein the leguminous green manure rotten material layer and the compound bacteria agent soil layer are separated through thin soil; and covering the compound bacteria agent soil layer with phloem bark, and introducing earthwormsinto a planting region, so as to obtain a remediated transplanting region after soil remediation is finished. According to the soil ecological remediation method, the soil is ecologically remediated by virtue of a large amount of environment-friendly substances, so that the soil is relatively suitable for growth of the pistacia chinensis, and the soil ecological remediation method is very environment-friendly on the whole.

The invention discloses a plant sex identification method for pistacia chinensis bunge. The method comprises steps of performing chromosome film production by utilizing meristems of pistacia chinensis bunge plants, and staining and labeling to obtain karyotypes of the pistacia chinensis bunge plants, and achieving sex identification by observing karyotype differences of the pistacia chinensis bunge plants, thus solving a problem that a stable and effective sex identification method before the pistacia chinensis bunge blossoms and yields fruit is not existed at present. An important application prospect of the method provided by the invention is to achieve sex ratio adjustment of the pistacia chinensis bunge plants by sex identification of the pistacia chinensis bunge plants, especially by female and male identification during a seeding stage. The method provided by the invention has significant meaning for cultivation of high-yield pistacia chinensis bunge energy forest with a proper sex ratio, and for large improvement of productivity per area of the pistacia chinensis bunge.

The invention relates to a pistacia chinensis culture technology which carries out technical innovation on nursery seed acquisition and processing, seedling stage management, nurture management and insect disease prevention. The invention improves the germination rate of pistacia chinensis seeds, shortens the sprouting time of the seeds, improves the oil content of the seeds, adjusts and controls the survival rate of seedlings and the shapes of tender seedlings, obviously dwarfs trees in the growth period and has the advantages of easy seed picking, high oil yield, high dietary, medical and economic values, no pollution, and the like.

The invention discloses a PcBLFY (Pistacia chinensis Bunge blooming gene) and the cloning of the PcBLFY. According to the known LEAFY gene sequence of other plants, a degenerate primer is designed, the total RNA of the male pistacia chinensis bunge, the female pistacia chinensis bunge and the monoecious pistacia chinensis bunge serves as a template, the RT-PCR method is adopted to synthesize a first cDNA chain, and the 5'RACE and 3'RACE methods are adopted to clone the cDNA full-length sequence of the PcBLFY. According to the obtained cDNA sequence, a primer is designed, the RT-PCR method is adopted to obtain the LEAFY genes of which two sequences, one sequence and two sequences having single nucleotide polymorphism difference from the male pistacia chinensis bunge, the female pistacia chinensis bunge and the monoecious pistacia chinensis bunge. The content disclosed by the invention can be used for creating early flowering and fructifying transgenic breeding. The PcBLFY is mainly used for determinating the sex of different pistacia chinensis bunge plantsx and adjusting the sex proportion of the pistacia chinensis bunge energy forest so as to improve the yield dramatically.

The invention relates to the field of edible fungi cultivation and in particular to an agaric culture substrate and a preparation method thereof. The raw materials include straws, cow dung, folium cortex eucommiae, pistacia chinensis leaf, the seed of cowherb, the root of kudzu vine, soybean hulls, banana skin, wild carrot fruit and sodium carboxymethylcellulose. The method comprises the following steps: mixing the folium cortex eucommiae with water for soaking, heating until the water is boiling, adding the seed of cowherb, the root of kudzu vine and wild carrot fruit, continuously boiling for 15 minutes, cooling, adding the banana skin, heating at a constant temperature, adding sodium carboxymethylcellulose, uniformly stirring, and performing microwave treatment to obtain a product for later use; uniformly mixing the straws, soybean hulls, pistacia chinensis leaf and cow dung, fermenting, sterilizing, uniformly mixing the product for later use, airing until the moisture content of the system is 20-25%, and bagging, thereby obtaining the culture substrate. The culture substrate disclosed by the invention is balanced in nutrition, can meet nutrients needed by growth of agaric, and has high resistance to infectious microbes under the condition that the nutrition is balanced.

The invention relates to the field of edible fungus cultivation, and in particular relates to a mushroom cultivation material and a preparation method thereof. The mushroom cultivation material is prepared from the following raw materials: straw, radix curcuma residues, cow dung, chicken manure, zanthoxylum piperitum leaves, pistacia chinensis bunge leaves, shrimp shells and traditional Chinese medicine liquid. The preparation method comprises the following steps: firstly mixing the chicken manure and shrimp shells with the traditional Chinese medicine liquid uniformly, and sealing for fermenting for one day to obtain a fermentation product for standby use; then mixing and smashing the zanthoxylum piperitum leaves, pistacia chinensis bunge leaves, straw and radix curcuma residues, adding water for pre-wetting for 30 min, controlling the water content of a system to be 30%, and then adding cow dung for blending; and after natural fermentation for one hour, adding the fermentation product for blending, steaming, cooling, and bagging an obtained product to obtain the cultivation material. The cultivation material disclosed by the invention has balanced nutrition, and is beneficial for nutrition supply required for mushroom growth; the infectious microbe inhibiting ability of the cultivation material is also strengthened; and meanwhile, the cultivation material also has a slow-release effect, and is used for long-time mushroom supply.

The invention discloses a method for improving the capability of Xinjiang pistachio stocks for living through winter safely. In the last ten-day period of Match every year, when the body fluid of the stocks of pistacia chinensis bunge begins to flow, the stocks of pistacia chinensis bunge are required to be irrigated 10 to 15 days before grafting; and in grafting, the soil around the base part of the trunks of the stocks is dug to a general depth of 5 to 10 centimeters, and the stocks are grafted at the root stalk parts. The low-position grafting method of the invention can bury the stocks of pistacia chinensis bunge with low cold resistance underground, thereby effectively preventing low-temperature cold damage in winter. The method provides a safe guarantee for the large-area promotion and application of pistacia chinensis bunge serving as the Xinjiang pistachio stocks and therefore has a high application value.

The invention discloses a method for preparing biodiesel from pistacia chinensis bunge. The method comprises the following steps that a kieselguhr adsorbent is added for pretreating pistacia seed oil to obtain pure pistacia seed oil; the pure pistacia seed oil, methyl alcohol and a magnetic nanoparticle catalyst are added into a reaction kettle according to the weight ratio of 100:25-30:0.5-0.8 for an esterification reaction; biodiesel crude products are separated out, purification is carried out, and therefore the biodiesel is obtained. Due to the fact that the magnetic nanoparticle catalyst is introduced into the esterification reaction, the catalytic efficiency is high, the catalytic effect is significant, and therefore the reaction time is shortened, and the biodiesel yield is improved; the magnetic nanoparticle catalyst can conveniently and rapidly separate and recycle the product according to an external magnetic field, and can be repeatedly recycled. In addition, the method has the advantages that the preparation technology is simple, energy conservation and environment protection are achieved, the reaction time is short, the yield is high, and the product purity is high, has the good social benefits, ecological benefits and economic benefits, and can achieve large-scale industrial production easily.

The invention relates to a processing method of dried pistacia chinensis bunge. The method comprises the following steps: picking tender shoots of pistacia chinensis bunge, cleaning, performing fixation, drying, sterilizing, and packaging. Pistacia chinensis bunge is widely distributed in China, is a conventional medicinal plant with homology of medicine and food, and also is one of energy forest tree species with development potential. The pistacia chinensis bunge shoots have the effects of reducing blood pressure, blood sugar and blood fat, inhibiting bacteria, resisting viruses, mutation and radiation, preventing cancers, resisting oxidation and ageing, improving immunity and the like; 3,3-bipolymer-[3,4-dihydrogen-4-(4'-hydroxyphenyl)]-7-hydroxycoumarin of 4-aryldihydrocoumarin in the pistacia chinensis bunge shoots also has an effect of phytoestrogen (such as soy isoflavone, lignin and soybean agglutinin) and can balance the estrogen level in bodies, prevent osteoporosis and cancers, especially breast cancers, prostate cancers and the like, relieve climacteric syndrome, reduce the content of cholesterol in bodies and reduce an occurrence rate of cardiovascular diseases such as hypercholesteremia and atherosclerotic lesion.

The invention belongs to the technical field of plant biology, particularly relating to a method for rapidly and efficiently rooting pistacia chinensis bunge by taking stem tips and stem segments as explants. In the method, inoculated culture comprises the following specific operation steps of inoculating disinfected and rinsed seeds on an MS culture medium without any hormone, wherein the culture conditions are as follow: 20 to 30 DEG C of temperature, 1,500 to 2,500 lx of illumination intensity and 14 h/d of illumination time; explant culture comprises the following specific operation steps of taking pistacia chinensis bunge seed seedlings; and inoculating the stem tips and the stem segments which are cut off on an ultra-clean workbench onto a rooting culture medium with 1/2 WPM inorganic salt, MS organic matter, 1.0 mg/L of IBA (Indole Butyric Acid), 0.2 mg/L of NAA (Naphthyl Acetic Acid), 30 g/L of sucrose and 6.8 g/L of agar powder, wherein the pH of the rooting culture medium is 5.8. According to the method, the rooting time of the pistacia chinensis bunge is further shortened, and a fast, high-efficiency, high-quality, wide-application range method for rooting the pistacia chinensis bunge is obtained.

The invention discloses a pistacia lentiscus seed lipase-producing endophyte PC1 and a separation method thereof. The separation method comprises the following steps: treating seeds by using alcohol and mercury chloride, then inoculating the seeds to beef extract peptone, culturing to obtain an endophyte, fermenting strains by using liquid LB, inoculating a bacterial liquid to a glycerol tributyrate solid culture medium for culturing, selecting the strains with transparent zones, inoculating the strains to a liquid LB culture medium for fermentation, performing centrifugal separation and filtration on a fermentation liquid, culturing a filtrate by using the glycerol tributyrate solid culture medium, and screening to obtain the strain PC1. A single colony is obtained by performing plate streaking on the strain PC1, the single colony has a white appearance and a wet and smooth surface in an early growth stage, then wrinkles are generated in edges, and the surface becomes dry; and the strain is positive after being subjected to Gram staining, and a thallus is rod-shaped under the observation of an electron microscope.

The invention discloses a processing method of pistacia chinensis bunge black tea. The pistacia chinensis bunge black tea is prepared by compounding dry pistacia chinensis bunge extract powder with black tea according to a mass ratio of 1 : (50-100). The processing method comprises the following steps: (1) proportioning the dry pistacia chinensis bunge powder and the black tea according to the mass ratio of 1 : (50-100); (2) performing mixing adsorption on the dry pistacia chinensis bunge powder and the black tea; (3) drying a mixture at 50-60 DEG C until the water content is not more than 7%; (4) packaging to obtain the pistacia chinensis bunge black tea. The processing method has the beneficial effects that the pistacia chinensis bunge leaf or bud extract is mixed with the black tea to prepare the pistacia chinensis bunge black tea; the medicinal value of pistacia chinensis bunge can be organically combined with the function of the black tea, the difficulty of separate tea making of pistacia chinensis bunge leaves is overcome, and the sour and astringent mouth feel and the medicinal fragrance of the pistacia chinensis bunge during separate tea making can be improved.

The invention belongs to the technical field of biodiesel preparation and particularly relates to a special production line and method for the cold pressing extraction of pistacia chinensis seed oil.The production line sequentially comprises a washing tank, a lifting conveying device, a horizontal conveying device and a spiral oil pressing device in the running direction of materials, wherein thelifting conveying device and the horizontal conveying device both adopt a mesh belt type conveying structure; a storage bin arranged at the feeding end of the lower part of the lifting conveying device is located at the bottom in the washing tank, and the side wall of the washing tank is provided with a material pushing plate and a forward and backward translation driving mechanism; an upper blowing mechanism and a lower blowing mechanism are arranged above and below a conveying mesh belt of the horizontal conveying device, and the discharging end of the horizontal conveying device is locatedabove a feeding opening of the spiral oil pressing device; and in the spiral oil pressing device, a plurality of thread spiral sleeves are fixedly arranged on the surface of a spiral main shaft in asleeving manner, the surface of a bar cage is provided with a cooling coiled pipe, one end of the cooling coiled pipe is provided with a refrigerant inlet, the other end is provided with a refrigerantoutlet, and a filter net is fixedly arranged at the upper part in an oil collecting groove. By optimizing the technology and the structure, labor can be saved, the production efficiency can be increased, the oil yield is high, the output is high, and oil quality is great.

The invention discloses a ferula bungeana insecticidal acaricide and a preparation method thereof. The prepared ferula bungeana insecticidal acaricide is formed by a ferula bungeana extract serving as a main component and auxiliary ingredients. The ferula bungeana insecticidal acaricide can be missible oil or a microemulsion, has a good pesticide effect and is safe to the environment, humans, livestock, natural enemies of pest mites and other beneficial organisms, and pest mites are unlikely to generate drug resistance to the pistacia chinensis acaricide. The insecticidal acaricide manufacturing technology is simple, low in cost and suitable for popularization and application.