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Plant sex identification method for pistacia chinensis bunge

An identification method and technology of Pistacia chinensis, applied in the field of agricultural biology, can solve the problems of long childhood, excess male plants, low area yield, etc., and achieve the effect of increasing yield

Inactive Publication Date: 2014-01-22
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Pistacia chinensis is a dioecious plant. The ratio of male and female plants in natural secondary forests is about 1:1, and about 10% of male plants can ensure sufficient pollen supply. Therefore, there are excess male plants in natural secondary forests, and the female plants that can bear fruit The proportion is low, and at the same time, Pistacia chinensis has a long childhood period, and it takes 8 to 12 years to bloom and bear fruit. However, there is no effective identification method for male and female plants before flowering and fruiting, resulting in low yield per unit area of ​​Pistacia chinensis.

Method used

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  • Plant sex identification method for pistacia chinensis bunge
  • Plant sex identification method for pistacia chinensis bunge
  • Plant sex identification method for pistacia chinensis bunge

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. Chromosome preparation:

[0036] 1) Fix the root tip tissue in Carnoy's fixative solution for 2 days, and then transfer it to 75% ethanol for later use;

[0037] 2) Use tweezers to take a small piece of tissue, put it on a glass slide, drop a small drop of acetic acid with a volume fraction of 50%, cover it with a cover glass, and press lightly with a dissecting needle to disperse the material into a thin layer. On the baking sheet, flatten the coverslip with your thumb;

[0038] 3) Observe under a phase-contrast microscope, select the slices with clear chromosomes, complete quantity, uniform dispersion, and no breakage and overlap, store in a -80°C refrigerator, and freeze for more than 12 hours for later use.

[0039] 2. Giemsa staining:

[0040] a. Take out the stored slides, quickly uncover the cover slip, soak in 100% alcohol overnight, then soak in 0.2M HCl in a 60°C water bath for 3 minutes;

[0041] b. Put it in a dyeing vat equipped with distilled water, ...

Embodiment 2

[0048] 1. Chromosome preparation:

[0049] 1) Fix the root tip tissue in Carnoy's fixative solution for 1 day, and then transfer it to 50% ethanol for later use;

[0050] 2) Use tweezers to take a small piece of tissue, put it on a glass slide, drop a small drop of 30% acetic acid by volume, cover it with a cover glass, and press lightly with a dissecting needle to disperse the material into a thin layer. On the baking sheet, flatten the coverslip with your thumb;

[0051] 3) Observe under a phase-contrast microscope, select the slices with clear chromosomes, complete quantity, uniform dispersion, and no breakage and overlap, store in a -80°C refrigerator, and freeze for more than 12 hours for later use.

[0052] 2. Giemsa staining:

[0053] a. Take out the stored preparation, quickly uncover the cover glass, soak in 100% alcohol overnight, then soak in 0.1M HCl in a 40°C water bath for 2 minutes;

[0054] b. Put it in a dyeing vat equipped with distilled water, and rinse i...

Embodiment 3

[0061] 1. Chromosome preparation:

[0062] 1) Fix the shoot tip tissue in Carnoy's fixative solution for 3 days, and then transfer it to 80% ethanol for storage for later use;

[0063] 2) Use tweezers to take a small piece of tissue, put it on a glass slide, drop a small drop of acetic acid with a volume fraction of 60%, cover it with a cover glass, and press lightly with a dissecting needle to disperse the material into a thin layer. On the baking sheet, flatten the coverslip with your thumb;

[0064] 3) Observe under a phase-contrast microscope, select the slices with clear chromosomes, complete quantity, uniform dispersion, and no breakage and overlap, store in a -80°C refrigerator, and freeze for more than 12 hours for later use.

[0065] 2. Giemsa staining:

[0066] a. Take out the stored slides, quickly uncover the cover glass, soak in 100% alcohol overnight, then soak in 0.3M HCl in a 70°C water bath for 5 minutes;

[0067] b. Put it in a dyeing vat equipped with dis...

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Abstract

The invention discloses a plant sex identification method for pistacia chinensis bunge. The method comprises steps of performing chromosome film production by utilizing meristems of pistacia chinensis bunge plants, and staining and labeling to obtain karyotypes of the pistacia chinensis bunge plants, and achieving sex identification by observing karyotype differences of the pistacia chinensis bunge plants, thus solving a problem that a stable and effective sex identification method before the pistacia chinensis bunge blossoms and yields fruit is not existed at present. An important application prospect of the method provided by the invention is to achieve sex ratio adjustment of the pistacia chinensis bunge plants by sex identification of the pistacia chinensis bunge plants, especially by female and male identification during a seeding stage. The method provided by the invention has significant meaning for cultivation of high-yield pistacia chinensis bunge energy forest with a proper sex ratio, and for large improvement of productivity per area of the pistacia chinensis bunge.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a sex identification method for Pistacia chinensis plants. Background technique [0002] Pistacia chinensis Bunge (Pistacia chinensis Bunge) is an important perennial woody oil plant with a wide range of distribution. It can be planted on marginal lands such as barren mountains and wastelands. It is suitable for large-scale afforestation, and the oil content of its seeds is as high as 40%. Pistacia chinensis seed oil is a high-quality raw material for biodiesel. It is considered to be a priority tree species for future ecological energy forest construction and biodiesel industry. Therefore, increasing the production of Pistacia chinensis is of great significance to solve the problems of insufficient supply and high cost of bioenergy raw materials in my country. Due to the promising development prospects of Pistacia chinensis, there are more and more researches on Pistaci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/84G01N1/30
Inventor 吴丽芳杨鸣雷
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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