57 results about "Sex ratio" patented technology

The invention discloses an animal sex control method based on Rbmy gene editing and application. The method controls animal sex by using CRISPR / Cas9 system to specifically cut Rbmy gene on Y chromosome. Rbmy gene encodes a germ cell specific nucleoprotein, this protein is an important factor related to spermatogenesis, and by cutting Rbmy gene, it is possible to deactivate Rbmy gene, thus Y sperm or fertilized ovum with Y sperm is deactivated and cannot develop into a normal embryo, birth ratio of female animals is finally increased and sex control is achieved. Therefore, sex ratio of animal offspring can be controlled through the method so that the ratio of female animals with better economic value is increased and production efficiency and economic effect are greatly improved.

The invention discloses a food attractant for melon flies, which consists of the following components in weight percentage: 1 to 10 percent of a melon aroma substance, 10 to 25 percent of a solvent, 60 to 80 percent of a nutritional agent, and 1 to 10 percent of a preservative. The melon aroma substance can be a volatile aroma substance extracted from melons or other substances which also have melon aroma. The attractant has the advantages that the attractant has extremely strong attracting effect on adult female and male melon fruit flies (melon flies, pumpkin fruit flies), ensures that the attracted female and male proportion satisfies the natural population sex ratio of the melon fruit flies in vegetable gardens, can remarkably reduce the harm of egg laying of female fruit flies, achieve the aim of effectively controlling the melon flies and the pumpkin fruit flies, and is applicable to the nuisanceless or green good production of melon vegetables.

The invention discloses an artificial pairing and breeding method of platysternon megacephalum. The method comprises the steps of: provenance selection, domestication before pairing, setting of a breeding pool, pairing and breeding, collection and incubation of eggs and the like. The method takes full account of the ecological and biological characteristics of platysternon megacephalum, including ethological characteristics, diet characteristics, habitat characteristics, breeding ecological characteristics and the like. The method can effectively solve the major problems in the current artificial pairing and breeding of platysternon megacephalum, including the problems in seed selection, pairing, selection of pairing time, sex ratio, density, bait, setting of breeding and incubation environment and the like; the occurrence of impairment and death in the breeding mating process is obviously reduced, the egg laying rate of female platysternon megacephalum and the incubation rate of eggs are obviously increased, artificial efficient breeding of platysternon megacephalum is realized, healthy platysternon megacephalum seedlings are obtained, and a technical support is provided for expanding the breeding population of platysternon megacephalum, promoting species protection and developing large-scale breeding; and the method requires little human input, has low cost, is easy to operate, and is favorable for popularizing the artificial breeding of platysternon megacephalum.

The invention discloses a regulating method for controlling the sex ratio of the floral organs of jatropha curcas by chemical reagents, pertaining to the technical field of plant growth regulators. The method includes that (1) 40 percent ethephon water solution and gibberellin (GA3) are selected as the chemical agents; (2) the 40 percent ethephon water solution is prepared into the solution with a concentration of 500mg / L to 2000mg / L and the gibberellin is prepared into the solution with the concentration 75mg / L to 100mg / L; (3) the surviving plant which is over two years old and has the capacity of flowering and fruiting is sprayed with the reagent; (4) the whole plant of the jatropha curcas is sprayed with the ethephon water solution just before the flowering prior period; (5) management and caring are carried out after spraying; (6) the gibberellin is sprayed. The method solves the problem of the ratio of the male jatropha curcas flower and the female jatropha curcas flower and can increase the ratio of the male jatropha curcas flower and the female jatropha curcas flower by 10percent to 20percent and effectively promote the flowering and the fruiting of the jatropha curcas and the yield of an individual plant.

This invention is directed to methods for manipulating sex, reversing sex, changing sex ratio, or moderating crustacean androgenic gland peptide expression in crustaceans for producing a monosex culture. In one embodiment, the present method comprises the step of injecting a dsRNA for the gene of IAG into a male crustacean. In another embodiment, the present method comprises the step of injecting a dsRNA for the gene of IAG into a female crustacean that had acquired a spermatophore (a sperm sac) after mating with a male. In another embodiment, the present invention provides a method of producing a monosex culture of crustacean by introducing a recombinant IAG peptide into the female crustacean, thereby obtains male with altered sexual characteristics for mating with a normal female. In one embodiment, the crustacean is a shrimp or lobster.

The invention relates to a method for efficiently breeding blue crabs. The method comprises the following steps: performing intermediate breeding on II-stage baby blue crabs in an outdoor cement pit greenhouse; disinfecting the water for breeding through an ultraviolet lamp and mounting an oxygenating device in a breeding pond; feeding big disinfected brine shrimps at regular time; exchanging 15% water per day; after the II-stage baby blue crabs grow and become V-stage baby blue crabs, putting juvenile crabs into the breeding pond at a sex ratio of female to male being 3:1; after ending mating, fishing out the male crabs; continuously breeding the female crabs; and when the water temperature is lower than 10 DEG C, putting the female crabs into the outdoor cement pit greenhouse for temporarily breeding and putting into the market before and after the Spring Festival, thereby greatly increasing the breeding yield and economic benefit. According to the method provided by the invention, the juvenile crabs are prevented from being killed by enemies and severe environment; the use ratio of baits is increased and the happening of diseases is reduced; the breeding yield of the blue crabs is increased by 26.99% and the breeding benefit is increased by 2.39 times, by adopting the method provided by the invention; the method can be widely applied to the breeding for the blue crabs; the energy conservation and the environment protection are realized; and the economic benefit and the social benefit are obvious.

The invention discloses an artificial pairing and breeding method of Platysternon megacephalum. The method comprises the selection of species sources, domestication before pairing, setting of a breeding pond, artificial pairing, collection and hatching of turtle eggs. The method fully considers the ecological and biological characteristics of the Platysternon megacephalum, including the behavior, feeding habit, habitat, and breeding ecology. The main problems in artificial pairing and captive breeding of the Platysternon megacephalum can be effectively solved, including the breeding turtle selection, pairing, pairing stage selection, sex ratio, density, artificial food, setting of the reproduction and hatching environments, the occurrence of disabilities and fatal events during the pairing and mating process is significantly reduced; the egg laying rate of female turtles and the hatching rate of turtle eggs are both significantly improved. Artificial high-efficiency breeding of the Playsternon megacephalum is realized, and healthy turtle hatchlings can be obtained.

The invention discloses an artificial breeding method for Crocodylus siamensis. The method includes the steps of provenance selection, domestication before pairing, breeding place setting, manual pairing and Crocodylus siamensis egg collection and hatching. The main problems, about provenance selection, pairing, pairing period selection, sex ratio setting, density setting, bait preparation and breeding and hatching environment setting, of a present artificial breeding method for the Crocodylus siamensis can be effectively solved, damage and death in the breeding mating process are remarkably reduced, the spawning rate of the female Crocodylus siamensis is remarkably increased, the hatching rate of Crocodylus siamensis eggs is remarkably increased, efficient artificial breeding of the Crocodylus siamensis is achieved, healthy Crocodylus siamensis larvae are acquired, and solid technical supports are provided for expanding the breeding population of the Crocodylus siamensis, promoting species protection and developing large-scale breeding.

A method of improving cumulative embryo score may comprise administering an androgen to a human female, for example, DHEA, for at least about four consecutive months followed by harvesting and fertilizing oocytes and forming embryos. Between about 50 mg and about 100 mg of DHEA may be administered to a human female per day. Moreover, a method of increasing the quantity of fertilized oocytes in one cycle of in vitro fertilization may comprise administering an androgen to a human female for at least about four consecutive months, harvesting and fertilizing the oocytes. Furthermore, a method of increasing the quantity of day 3 embryos from one cycle of in vitro fertilization may comprise administering an androgen for at least about four consecutive months, harvesting and fertilizing the oocytes and forming day 3 embryos. A method of normalizing ovarian DHEA also may include administering an androgen for at least about four consecutive months. A method of increasing the rate and number of euploid oocytes may include administering an androgen for at least about four consecutive weeks. In addition, a method of increasing male fetus sex ratio may comprise raising baseline androgen levels in a female prior to or at time of embryo implantation.

The invention relates to a manufacture method of continuous paraffin sections for gonad of pelodiscus sinensis in embryonic period and application of the manufacture method in sex determination. The manufacture method comprises the following steps: (1) fixing a sample, (2) dying the gonad, (3) carrying out dehydration and transparentizing procedure, (4) absorbing paraffin and embedding, (5) cutting into sections and flatting sections, and (6) dying and mounting. The manufacture method is characterized in that the gonad is first dyed in the step (2); sections are subjected twice flatting by adopting water bath and high-temperature paraffin in the step (5). Compared with the prior art, the manufacture method has the advantages that the gonad is dyed first before being embedded, the gonads of a plurality of individuals are embedded in one paraffin block, determination is carried out through a manner of continuous tissue sections, and then the result accuracy is ensured. The manufacture method is simple to operate and convenient to popularize and apply. By utilizing the manufacture method, sex ratio of the pelodiscus sinensis in the embryonic period can be rapidly and accurately subjected to statistics.

The invention discloses application of cytokinin 6-BA in changing sex ratio of plant flowers. In the application, the cytokinin 6-BA is used for regulating the sex ratio of jatropha curcas flowers and increasing the proportion of male and female jatropha curcas flowers. Under a certain condition, the spraying concentration of the cytokinin 6-BA influences the sex ratio of the male flowers to the female flowers and is between 20 mg / L and 100 mg / L, wherein the certain condition is that the age of the plant tree is more than 2 years; the spraying method is to spray the cytokinin 6-BA on the whole jatropha curcas at the bud stage of the first flowers, wherein the drips are small and uniform at the time of spraying, the spraying amount is enough when the sprayed part is wet and fine liquid drops fall, and the spraying time is between 14:30 and 16:30 p.m. in a sunny day; and if rain falls within 36 hours after spraying, the cytokinin 6-BA needs to be sprayed again. The invention solves the problem that the proportion of the male and female jatropha curcas flowers is low and can increase the proportion of the male and female jatropha curcas flowers by 15.89-67.48% and the individual yield by 4-31%.

A purified antibody, or an antigen-binding fragment thereof, is provided that binds selectively to a protein specific to an X-chromosome of a mammalian sperm cell. The sperm cell protein comprises an amino acid sequence set forth in SEQ ID NOs: 4 and 9-16. The antibody or antigen-binding fragment thereof may be derived by immunization of a host by an antigenic peptide composition comprising one or more natural or synthetic antigenic peptide sequences set forth as SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, and SEQ ID NOs: 4 and 9-16. The antibody finds utility in identifying an X-chromosome bearing sperm cell population, and in methods for skewing a sex ratio in mammals.

The invention discloses a method of hatching Yellow throat quasi-water tortoise, in the breeding season, employing ventilating plastic box as hatching box, laying a 3-6cm -thick sand grain layer on the box bottom as hatching base material, putting the fertilized ovum into the box, 2-3 fertilized ovum for every 100cm2, covering a 1-3cm-thick sand grain layer, the water content of the hatching bed during the whole hatching period is maintained 5-10%; and the temperature is controlled in 28-30 Deg C, then getting the infant tortoise with balanced sex ratio after 65-70 days later. The invention can increase the success rate of hatching Yellow throat quasi-water tortoise, and the survival rate of the infant tortoise.

The invention discloses a method for controlling hoplobatrachus tigerinus sex ratio and application of the method and belongs to the technical field of hoplobatrachus tigerinus artificial culture. The method includes: placing hatched tadpoles into a cultivation pond according to cultivation density of 3-4 / liter, using feed containing 0.08-0.12 milligram of testosterone or letrozole in each gram to feed the tadpoles under 29-34 DEG C for two weeks, and then using feed which does not contain the testosterone or letrozole to feed the tadpoles until metamorphosis is completed. By the method, the sex ratio after the metamorphosis can be adjusted effectively, and the cultivation efficiency of hoplobatrachus tigerinus can be increased greatly. Compared with feeding under natural conditions, the method has the advantages that a metamorphosis cycle can be shortened greatly, and metamorphosis rate can be increased.

The invention discloses a PCR (Polymerase Chain Reaction) method for the sex appraisal of Nipponia nippon, and provides a primer pair for detecting the sex of a bird. In the primer pair for detecting the sex of a bird, one primer is the nucleotide disclosed as sequence 1 in the sequence list, and the other primer is the nucleotide disclosed as sequence 2 in the sequence list. Experiments provided by the invention prove that the sex of Nipponia nippon can be conveniently and accurately appraised based on different banding patterns produced by PCR amplification by using cross-intron primers designed on the basis of the length difference between Z and W chromosomes of CHD gene introns. The technology is of far reaching importance in the sex ratio monitoring of wild young Nipponia nippon, the construction and reintroduction of the scientific pairing system of breeding population as well as the selection and reasonable collocation of released individuals.

Described herein are embodiments relating to manipulation of populations and sex ratio in populations through DNA sequence modifications.

The invention provides a method for improving development quality of in vitro fertilization (IVF) female embryos after implantation and correcting sex ratio of IFV embryos. The method comprises: transferring mouse fertilized embryos obtained by IVF technology into a culture solution added with low-dose retinoic acid (RA) for short-term culturing, a female specific apparent modification event (namely x-chromosomal inactivation) in early development of IFV embryos is established normally so that an abnormal development ratio of mouse IFV female embryos after implantation is reduced and the mouse IVF birth sex ratio imbalance problem is finally corrected. In addition, the method is successfully applied in a bovine IVF system, effectively increasing development rate of bovine IFV female blastulas and correcting the sex ratio of bovine IFV blastulas. The RA in the invention is used as a fallopian tube to widely express an endogenous active matter, this matter is suitably added in the IVF system with endogenous ingredients not blindly added, and application safety is greatly ensured.

The invention provides a method for mass-producing parasitic wasps of fruit flies by means of drosophila melanogaster. By fast and simply collecting pupae of the drosophila melanogaster, mass breedingand parasitism of the parasitic wasps of the fruit flies are simply completed, in a pupal stage, the parasitic wasps of the fruit flies are fast and simply separated from the unparasitized drosophilamelanogaster, and the number and a sex ratio of the parasitic wasps of the fruit flies are fast and simply regulated and controlled, so that full mating and a proper parasitism rate are ensured.

The invention discloses a plant sex identification method for pistacia chinensis bunge. The method comprises steps of performing chromosome film production by utilizing meristems of pistacia chinensis bunge plants, and staining and labeling to obtain karyotypes of the pistacia chinensis bunge plants, and achieving sex identification by observing karyotype differences of the pistacia chinensis bunge plants, thus solving a problem that a stable and effective sex identification method before the pistacia chinensis bunge blossoms and yields fruit is not existed at present. An important application prospect of the method provided by the invention is to achieve sex ratio adjustment of the pistacia chinensis bunge plants by sex identification of the pistacia chinensis bunge plants, especially by female and male identification during a seeding stage. The method provided by the invention has significant meaning for cultivation of high-yield pistacia chinensis bunge energy forest with a proper sex ratio, and for large improvement of productivity per area of the pistacia chinensis bunge.

The invention discloses transsexual feed for cynoglossus robustus and a preparation method of the transsexual feed. The feed is prepared from the following components in percentage by mass: 20-60% offish meal, 10-30% of protein hydrolysate, 3-10% of beer yeast, 10-30% of wet artemia, 5-10% of shrimp flesh, 5-10% of egg powder, 15-30% of flour, 1-5% of fish oil, 1-3% of phospholipid, 1-3% of glycine betaine, 1-2% of a vitamin mixture, 2-4% of a mineral element mixture, 1-5% of a microbial preparation, 1-3% of a compound immunopotentiator, 1-5% of copepod, 1-3% of casein and 1-2% of a phagostimulant. The transsexual feed for cynoglossus robustus disclosed by the invention can achieve the effects of promoting the development of sexual glands, promoting male-to-female transformation of the cynoglossus robustus, regulating the sex ratio of the cynoglossus robustus cultured population, reducing the ratio of male cynoglossus robustus in the cultured population, increasing the ratio of femalecynoglossus robustus, improving the survival rate, improving the feed conversion ratio and specific growth rate, obviously increasing the aquaculture field of the cynoglossus robustus and reducing the culturing cost and has significances for promoting cynoglossus robustus fries and developing the breeding industry of the cynoglossus robustus.

The present invention relates generally to genetic sequences and their complementary forms capable of facilitating the modification of a phenotype of a plant. More particularly, the present invention relates to polynucleotide sequences defining B-type genes, to the proteins encoded thereby, to methods for isolating such polynucleotides and to nucleic acid constructs for the expression thereof. The present invention further provides cells, particularly transformed bacterial or plant cells and to differentiated tissue including whole plants and their progeny comprising cells which contain these nucleic acid constructs or parts of the constructs. Plants and parts of plants, such as flowering and reproductive parts including seeds, also form part of the present invention. The genetic sequences of the present invention may be used inter alia for the production of plants and, in particular, oil palm plants, which have modified phenotypes and / or which exhibits more highly desired characteristics such as, for example, male sterility or plants in which the sex ratio may be manipulated, and for the diagnosis and, preferably, elimination of the mantled phenotype.

The invention relates to the technical field of genetic breeding of silkworms, in particular to a silkworm breeding method capable of realizing whole male silkworms after two successive generation hybridization through controlling a sex ratio. According to the method, a silkworm sex-linkage balanced lethal system and a Z chromosom linkage condition lethal system are used, and through hybridized combination of two strains, F1-genearion female silkworms are all dead, F1-genearion male silkworms are all survived, after the F1-genearion male silkworms hybridize with other conventional female silkworms, F2-genearion female silkworms are all dead, and F2-genearion male silkworms are all survived, so that whole male silkworms after two successive generation hybridization is realized. By adopting the method, the silkworm breeding cost is reduced and economic benefits for silkworm breeding are increased.

The invention discloses a new use of KT (Kinetin) in plant growth regulation. The KT is used for sex ratio regulation of barbadosnut floral organs to improve the ratio of female flowers to male flowers of the barbadosnut. Under a certain condition, the concentration of sprayed KT ranges from 1mg / L to 5mg / L, wherein the condition refers to barbadosnut age of more than two years; the whole branches of the barbadosnut in a first flower bud period is sprayed; sprayed droplets are fine and uniform; the limit of dosage requires that a sprayed part is wetted and fine droplets drop; the spraying time ranges from 14:30 to 16:30 in the afternoon in a sunny day; supplementary spraying is required if a rain day comes in 36 hours after spraying; and the optimal spraying concentration of the KT is 3mg / L. The invention solves the problem of low ratio of female flowers to male flowers of the barbadosnut and can improve the ratio of female flowers to male flowers of the barbadosnut by 14.80 percent to 36.18 percent, effectively improve the yield o single barbadosnut and achieve the purpose of yield increase.

The invention belongs to the field of plant biology, and relates to a method for determining the sex of an oil palm immature inflorescence by a cytohistology technique. The method comprises the steps: selecting an inflorescence in III period of a male and female non-dominant period, carrying out fixation, dehydration and transparence, wax dipping and embedding, slicing and baking, and dewaxing and staining to prepare a section, observing and photographing the section with a microscope, calculating the number of flower bract primordia, the number of flower bracts, the number of new flower bract primordia and the number of floral primordium meristems, and then determining the sex of the inflorescence according to a determining ruler of the sex of the oil palm immature inflorescence. According to the difference of oil palm male and female inflorescences in morphology and cytohistology levels, the sex of the inflorescence is determined in the male and female non-dominant period in advance by using the cytohistology technique, the research time is effectively shortened, the research process is accelerated, the basis is provided for further researching the relationship of an oil palm inflorescence sex differentiation mechanism with external factors, and important significance on flowering phase and sex ratio regulation, yield increasing and the like in oil palm production is achieved.

The invention belongs to the technical field of livestock thremmatology, and particularly discloses a livestock open nucleus building method.An open nucleus method is integrally built by synthesizing nucleus breed swine sources, population structure, sex ratio, generation interval control, active and passive elimination, breed swine classified management, heritable variation monitoring and the like, a standard digital swine breeding and production management system is built, and the breed swine genetic improvement value is maximized.

The invention discloses novel application of an auxin IAA. In the novel application, the auxin IAA is used for regulating jatropha curcas flower organ sex ratio and enhancing the proportion of male and female flowers of jatropha curcas, particularly, under a certain condition, the spraying concentration of the auxin IAA influences the sex ratio of the male and the female flowers, and a spraying concentration range of the auxin IAA is 20-100mg / L, wherein certain condition is that a plant age is above 2 years, and a spraying method comprises the steps of spraying the whole jatropha curcas plant in a first harvest flower bud stage, wherein drops are fine and uniform in the spraying process, the quantity is limited to that a sprayed part is wet with micro drops flowing down, the spraying time is from two thirty to four thirty at afternoon at a sunny day; and spraying again if raining within 36h after spraying, wherein the optimal spraying concentration of the auxin IAA is 60mg / L. The invention can ensure that the proportion of the male flowers to the female flowers of the jatropha curcas is increased by 14.56-35.89 percent and the yield per plant is enhanced by 9-69 percent.

The invention discloses a method for controlling hoplobatrachus tigerinus sex ratio and application of the method and belongs to the technical field of hoplobatrachus tigerinus artificial culture. The method includes: placing hatched tadpoles into a cultivation pond according to cultivation density of 3-4 / liter, using feed containing 0.08-0.12 milligram of testosterone or letrozole in each gram to feed the tadpoles under 29-34 DEG C for two weeks, and then using feed which does not contain the testosterone or letrozole to feed the tadpoles until metamorphosis is completed. By the method, the sex ratio after the metamorphosis can be adjusted effectively, and the cultivation efficiency of hoplobatrachus tigerinus can be increased greatly. Compared with feeding under natural conditions, the method has the advantages that a metamorphosis cycle can be shortened greatly, and metamorphosis rate can be increased.

The invention discloses a PCR (Polymerase Chain Reaction) method for the sex appraisal of Nipponia nippon, and provides a primer pair for detecting the sex of a bird. In the primer pair for detecting the sex of a bird, one primer is the nucleotide disclosed as sequence 1 in the sequence list, and the other primer is the nucleotide disclosed as sequence 2 in the sequence list. Experiments providedby the invention prove that the sex of Nipponia nippon can be conveniently and accurately appraised based on different banding patterns produced by PCR amplification by using cross-intron primers designed on the basis of the length difference between Z and W chromosomes of CHD gene introns. The technology is of far reaching importance in the sex ratio monitoring of wild young Nipponia nippon, theconstruction and reintroduction of the scientific pairing system of breeding population as well as the selection and reasonable collocation of released individuals.

The invention discloses a method for early classification of unbalanced multivariate time series data. First, to solve the problem of unbalanced data size between classes, according to the imbalance ratio, the undersampling of the large class category data set is divided into multiple subsets, which are combined with the small class category data to form multiple sub-training sets. Secondly, the kernel features are extracted and selected for each sub-training set, and a rule-based sub-classifier is constructed using the kernel features. Among them, in order to solve the problem of unbalanced data scale of sub-concepts within a class, the feature selection process is implemented by clustering to ensure the diversity of kernel features. Finally, based on each sub-classifier, the weight of the classification effect of the sub-classifier on the data in the training set is calculated to construct an integrated classifier. The classifier of the present invention can aim at the multivariate time series classification problem of the unbalanced data set, and achieve higher accuracy and better earlyness.