11 results about "Mammalian sperm" patented technology

To provide a sperm diluent capable of obtaining a sperm population rich in X-chromosome-bearing sperm with acrosome retention by a simple method, to provide a method for preparing mammalian sperm, to provide an artificial insemination method, and to provide an in vitro fertilization method.SOLUTION: A sperm diluent induces an acrosome reaction in Y chromosome-bearing sperm to obtain a sperm population rich in X chromosome-bearing sperm that retain an acrosome and contains a TLR7 ligand and has a citric acid content of 4 mM or less. A method for preparing mammalian sperm comprises inducing an acrosome reaction in Y chromosome-bearing sperm by culturing with the sperm diluent to obtain a sperm population rich in X chromosome-bearing sperm that retain an acrosome.SELECTED DRAWING: Figure 3

The present invention is based on wild-type histone methyltransferase (SETDB1), obtains a SETDB1 mutant by site-directed mutagenesis technology, and verifies that the SETDB1 mutant can further improve the number, vitality and survival index of pig sperm compared with wild-type SETDB1 and the control group. At the same time, the SETDB1 mutant is used to treat boar semen / spermatocysts, and PDRN is prepared after inducing sperm cell maturation. The prepared PDRN has a higher extraction rate and purity than no treatment or treatment with wild-type SETDB1. The SETDB1 mutant of the present invention provides a new idea for improving the number, vitality, and survival index of sperm in mammals such as pigs and industrial preparation and production of PDRN, showing a wide range of application potential.

The invention discloses application of lycopene in preparation of medicines for treating hidden testis, and belongs to the technical field of biological medicines. As a naturally existing carotenoid, the lycopene can effectively improve spermatogenesis of a mouse suffering from cryptorrhea, and the research result provides a solid foundation for treatment of a patient suffering from cryptorrhea by the lycopene; the lycopene regulates and controls the spermatogenesis of the hidden testis through a hedgehog signal channel by reducing the level of active oxygen in tissues. The invention verifies the effects of lycopene on the aspects of reproductive system, influence on spermatogenesis, sperm motility protection and the like, and aims to verify factors influencing spermatogenesis and the action mechanism of lycopene on spermatogenesis of mammals. And a reliable theoretical basis is provided for the application of the gene in the aspects of excellent breeding of large animals, even male infertility and the like of human beings.

The application belongs to the technical field of gene diagnosis, and discloses a new pathogenic gene CYLC1 of sperm head deformity and a mutation detection reagent thereof; the new pathogenic gene CYLC1 of sperm head deformity is used as a candidate gene diagnosis object of sperm head deformity, and four homozygous missense mutations thereof are c.1157A>C / p.N386T, c.1377G>T / p.K459N, c.1402T>G / p.S468A and c.1834T>A / p.C612S. The application can use the detection reagent to perform simple, fast and accurate gene diagnosis on sperm head deformity patients, and is also helpful for understanding pathogenesis, genetic counseling, prenatal diagnosis and gene therapy. The application can also use the established Cylc1 gene knockout mouse model to reveal the role and mechanism of Calicin-1 protein on sperm acrosome anchoring and head development of mammals.

The present disclosure relates to a mesoscale fluidic device for separating motile cells from non-motile cells, the mesoscale fluidic device including a substrate having a sample application compartment below a medium compartment, and a cell permeable filter with a pore size in the range of 1 μm to 20 μm, the cell permeable filter having an upper surface facing the medium compartment and a lower surface facing the sample application compartment, with the sample application compartment and the medium compartment being in fluid communication via the cell permeable filter, wherein the sample application compartment has a floor and at least one flow structure extending from the floor towards the lower surface of the cell permeable filter facing the sample application compartment. The device is especially useful for obtaining a medium enriched in motile sperm cells from a mammal sperm sample.

In a method of preparing mammalian sperm, mammalian sperm are cultured in a medium at a pH of 7.7 to 7.9 containing a TLR7 ligand, to induce acrosome reaction of Y-chromosome-bearing sperm, to obtain a sperm population rich in X-chromosome-bearing sperm retaining the acrosome. By subjecting the sperm population to artificial insemination or in vitro fertilization, gender preselection for obtaining male offspring is possible.

The invention relates to the field of biological detection and toxicological evaluation, and discloses an in-vitro detection method for screening or evaluating sperm motility toxicity of a compound. The method is realized by detecting a group of specific biomarkers in mammalian sperm treated by a tested compound, and the marker combination comprises a phosphoserine / threonine PKA substrate level, an AKAP3 protein level and an AKAP4 protein level. Researches show that environmental toxin fenvalerate can specifically reduce the levels of the markers by destroying cAMP / PKA signal channel, so that sperm motility is reduced. Through quantitative analysis of changes of the markers, whether a tested compound has a similar sperm direct toxicity effect or not can be specifically and efficiently evaluated, and a new tool is provided for chemical safety evaluation and male infertility pathogenesis diagnosis.

The invention belongs to the field of medicines for treating male infertility diseases, and discloses application of 12-ketolithocholic acid (12-keto-LCA) in the aspect of improving sperm quality decline, namely application of 12-keto-LCA in preparation of medicines for improving sperm quality decline. The invention provides a new application of 12-keto-LCA, reveals for the first time that 12-keto-LCA can support the spermatogenesis process, especially can improve the reproductive function of obese males, realizes the effects of improving testosterone secretion, repairing the spermatogenic function, improving the sperm quality and embryonic developmental ability and the like, and improves the condition that the sperm quality of mammals is reduced. The 12-keto-LCA can effectively relieve dyszoospermia of males, especially relieve damage of obesity to the reproductive function of males, and a new way is provided for developing drugs for improving the reproductive function of obese males.

The invention relates to an improved method for analysing sperm DNA structure, by means of which it is possible to reduce the time required to process samples by 80%, optimising every stage of the process. This effect is achieved through a synergetic improvement in each step of the process. The formation of microgels is accelerated with cold metal plates (2–8°C) and using 0.8%-1.5% agarose. DNA denaturation is carried out using HCl (100 mM). The deproteinisation solution contains DTT (0.22 M), NaCl (2.75 M), detergents of the alcohol ethoxylate (0.044 M) and SDS (0.0014 M) type. Fixation is carried out using 96% ethanol at a low temperature. The staining process uses a panoptic dye, improving the quality of the images. The invention not only speeds up the analysis by reducing the time required by 80%, but also enables faster and more accurate diagnoses, thanks to the high quality of the images obtained.

Provided are a method for separating mammalian sperm, by which even a large amount of sperm can be separated in a short time and decrease in motility of the separated sperm can be prevented; and an artificial insemination method, and an in vitro fertilization method. The method for separating mammalian sperm includes incubating the sperm in a medium containing a TLR7 ligand, and separating them into sperm floating in the upper layer and sperm sinking in the lower layer of the medium. The artificial insemination and in vitro fertilization methods include artificial insemination or in vitro fertilization for a non-human mammal using sperm of the non-human mammal separated by the method for separating mammalian sperm.