41 results about "Mammalian sperm" patented technology

The present invention provides a method of cryopreserving sperm that have been selected for a specific characteristic. In a preferred embodiment, the method is employed to freeze sex-selected sperm. Although the cryopreservation method of the invention can be used to freeze sperm selected by any number of selection methods, selection using flow cytometry is preferred. The present invention also provides a frozen sperm sample that has been selected for a particular characteristic, such as sex-type. In preferred embodiments, the frozen sperm sample includes mammalian sperm, such as, for example, human, bovine, equine, porcine, ovine, elk, or bison sperm. The frozen selected sperm sample can be used in a variety of applications. In particular, the sample can be thawed and used for fertilization. Accordingly, the invention also includes a method of using the frozen selected sperm sample for artificial insemination or in vitro fertilization.

Methods and compositions for inhibiting or preventing spermatogenesis in a male mammal. Also disclosed are compounds and formulations for use in such methods.

The present invention relates to a method and a system for producing a mammalian pre-embryo and a stem cell having a better quality than prior art methods. The system comprises means for obtaining a mammalian oocyte, and means for obtaining a mammalian spermatozoa, and an apparatus having at least two separate air-tight chambers, for which the oxygen tension of one chamber may be changed independent of the oxygen tension of the other chamber, said at least two separate air-tight chambers constitute a main chamber and at least one residence chamber. The method for in vitro producing a mammalian pre-embryo comprising the steps: a1) providing a mammalian oocyte, a2) providing a mammalian spermatozoa, b) culturing the oocyte and the spermatozoa, c) fertilizing the oocyte with the spermatozoa obtaining a fertilized oocyte, and d) allowing cell-division of the fertilized oocyte obtaining a multicellular pre-embryo wherein at least one of the steps a1) or a2) is conducted at an oxygen tension below 15%, or e) allowing cell-division of the fertilized oocyte obtaining a multicellular pre-embryo, wherein the culture is performed at an oxygen tension allowing cultivation of the cells and wherein at least one of the steps comprises a change in the oxygen tension. Stem cells are produced from the multicellular pre-embryo.

The present invention provides an aptamer or pool of aptamers that bind(s) to a target molecule on the surface of a mammalian sperm cell and a method for producing the aptamers. The method comprises contacting a collection of different nucleic acid molecules with the target molecule under conditions favorable for binding between at least one of the nucleic acid molecules and the target molecule, to form at least one complex comprising the nucleic acid molecule bound to the target molecule, wherein each of the nucleic acid molecules comprises at least one segment of randomized nucleotide sequences. The complexes are then separated from the unbound nucleic acid molecules and unbound target molecules, and the bound nucleic acid molecule is recovered from the separated complex. The aptamers are used to separate sperm cells based upon sperm cell qualities, such as whether the cells contain X chromosomes or Y chromosomes.

The invention discloses a fluorescent staining method for evaluating sperm motility of domesticated mammals. The fluorescent staining method comprises the following step: according to the characteristic that a mammal live sperm probe Hoechst 333422 and a mammal dead sperm probe PI can be excited by the same ultraviolet light to emit fluorescent light, performing fluorescent staining on sperms of four important domesticated mammals which are pigs, cows, sheep and dogs to accurately perform sperm mobility evaluation. Compared with the conventional PI/SYBR-14 staining method, the PI/Hoechst 33342 staining method adopts Hoechst 33342 to substitute an expensive SYBR-14 probe, so that the detecting cost is greatly lowered. The fluorescent staining method can be used for successfully evaluating the sperm mobility of important domesticated mammals such as pigs, cows, sheep and dogs, and can be widely applied to evaluating the sperm motility of various domesticated mammals.

A purified antibody, or an antigen-binding fragment thereof, is provided that binds selectively to a protein specific to an X-chromosome of a mammalian sperm cell. The sperm cell protein comprises an amino acid sequence set forth in SEQ ID NOs: 4 and 9-16. The antibody or antigen-binding fragment thereof may be derived by immunization of a host by an antigenic peptide composition comprising one or more natural or synthetic antigenic peptide sequences set forth as SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, and SEQ ID NOs: 4 and 9-16. The antibody finds utility in identifying an X-chromosome bearing sperm cell population, and in methods for skewing a sex ratio in mammals.

The invention relates to a La-1-like peptide agent that increases sperm motility. It is non-toxic and it may be used to treat male infertility in humans, and also to increase fertility in animals. It may be used in artificial reproductive techniques such as in vitro fertilisation (IVF), including intra-cytoplasmic sperm injection (ICSI). The La-1 like peptide agent may also be used in artificial insemination techniques such as intra-cytoplasmic uterine injection (IUI).

An apparatus for microfluidic flow cytometry analysis of a particulate containing fluid An apparatus for microfluidic flow cytometry analysis of a particulate containing fluid comprises a hydrodynamic focussing apparatus for providing a focused stream of particulate containing fluid; and a microfluidic chip. The chip has a plurality of layers and comprises a microfluidic channel that extends through the chip substantially orthogonal to a plane of the layers of the chip, and is in fluid communication with the hydrodynamic focusing apparatus for receipt of a focused steam of particulate containing fluid. The chip also comprises a detection zone comprising at least one pair of electrodes in electrical communication with the microfluidic channel. At least one pair of electrodes comprise an excitation electrode coupled to an AC signal source and a detection electrode configured to detect AC impedance changes in the microfluidic channel between the electrodes resulting from particles passing between the electrodes in the microfluidic channel. Methods of sorting mammalian sperm cells according to sex is also described.

The present invention provides an aptamer or pool of aptamers (nucleic acid sequences) that bind(s) to a target molecule on the surface, accessible from the surface or inside of a mammalian sperm cell and a method for producing the aptamers. The method comprises contacting a collection of different nucleic acid molecules with the target molecule under conditions favorable for binding between at least one of the nucleic acid molecules and the target molecule, to form at least one complex comprising the nucleic acid molecule bound to the target molecule, wherein each of the nucleic acid molecules comprises at least one segment of randomized nucleotide sequences. The complexes are then separated from the unbound nucleic acid molecules and unbound target molecules, and the bound nucleic acid molecule is recovered from the separated complex. The aptamers are used to separate sperm cells based upon sperm cell qualities, such as whether the cells contain X chromosomes or Y chromosomes.

Liposomes comprising an FKBP52 targeting agent (FTA) are disclosed. Pharmaceutical compositions comprising an FTA, a solvent, and a surfactant are disclosed. Pharmaceutical compositions comprising a cyclodextrin and/or a derivative thereof and an FTA are also disclosed. Method of detecting one or more compounds in a sample by liquid chromatography/tandem mass spectrometry (LC/MS/MS), methods of treating or preventing cancer, benign prostatic hyperplasia (BPH), prostatic intraepithelial neoplasia (PIN), prostatitis, enlarged prostate, or insulin-independent diabetes, and methods of inhibiting spermatogenesis or fertilized oocyte implantation in a mammal are also provided.

The invention relates to a sperm dilution medium that comprises a base medium to which are added yolk or yolk plasma, glycerol and native phosphocaseinate. The medium can particularly be used for the freeze-preservation of mammal sperm in order to enhance the fertilization capability of the frozen sperm.

A method for adjusting the maturation state of mammalian sperm for use in assisted reproductive technologies (ART) is disclosed. A mammalian ejaculate is provided and incubated under controlled conditions. Aliquots of the ejaculate are assayed during incubation period at intervals to determine maturation state and changes in the maturation state by observing the percent positive cells in the aliquot. The assays are repeated with successive aliquots at intervals during incubation to observe real time changes in the maturation state. The ejaculate remaining is processed for the desired ART after the percentage of positive cells in the latest aliquot being assayed begins to decline.

A composition of matter for increasing the motility and/or percentage of intact acrosomes (PIA) in sperm is described. The composition includes, in combination, an amount of FAA and an amount of TIMP-2, wherein the amounts are effective to increase the motility, the PIA, or the motility and PIA of sperm contacted with the composition. The composition can be used as a cryopreservation medium for sperm.

The present invention relates to an immunological method that selects spermatozoa having X-chromosome or Y-chromosome. The method of the invention is based on the use of monoclonal antibodies directed against the genus-specific proteins located in the cytoplasm membrane of spermatozoa associated to the action of the classical complement pathway to increase the percentage of one of the gender in the offspring of mammals.

Provided are a method for separating mammalian sperm, by which even a large amount of sperm can be separated in a short time and decrease in motility of the separated sperm can be prevented; and an artificial insemination method, and an in vitro fertilization method. The method for separating mammalian sperm includes incubating the sperm in a medium containing a TLR7 ligand, and separating them into sperm floating in the upper layer and sperm sinking in the lower layer of the medium. The artificial insemination and in vitro fertilization methods include artificial insemination or in vitro fertilization for a non-human mammal using sperm of the non-human mammal separated by the method for separating mammalian sperm.

The invention belongs to the biotechnology field and discloses a use of an epididymis specific expression gene HongrES1 or an agonist thereof or an antagonist in the preparation of compositions for regulating sperm capacitation of mammals; the invention further discloses a method for screening effective substances for regulating the sperm capacitation of the mammals by taking the epididymis specific expression gene as a target point. The invention firstly reveals that the HongrES1 has an important role on the aspect of regulating the sperm capacitation and the fertility of the mammals, thereby providing the effective target point for researching male reproduction.

The invention discloses an application of CLRN3 protein as cell surface identification protein for separating X sperms. The CLRN3 protein is in specific expression on the surface of the mammal X spermoblast, and an amino acid sequence of the CLRN3 protein is as shown in sequence 2 in a sequence table. The method for separating the X sperms provided by the invention is not only simple and easy to do, and the whole separation process is free from using dye and radiation, the pressure on the spermoblast is small, and the harmful influence on the sperm is relatively small. Through the method provided by the invention, the heavy demand on the sexed sperm in the production can be satisfied. The application disclosed by the invention has important application value.

The invention discloses a method for realizing animal sex control by performing gene editing on X chromosome multi-copy genes. The method comprises the following steps: screening and selecting one ormore multi-copy genes on an X chromosome of a mammal as target sites, and designing and synthesizing corresponding sgRNA; then, constructing a CRISPR/Cas9 expression system of a targeted X chromosomemulti-copy gene to cut the X chromosome in sperms or fertilized eggs of mammals; according to the method, the whole X chromosome can be inactivated, X sperms can be killed, the birth rate of male mammals can be increased, or XY type embryos can be killed, and the birth rate of female mammals can be increased. According to the method provided by the invention, the X chromosome cutting efficiency ishigh, the off-target rate is low, and a good sex control effect is achieved.

A method for increasing the fertilizing capacity of sperm cells, which method includes irradiating sperm cells with non-coherent red light in a discontinuous manner according to a pattern that includes at least one sequence of two irradiation periods of specific durations, which are separated by an intermediate period of darkness of a specific duration. Each of the specific periods of irradiation and darkness lasts between 8 and 15 minutes.

The invention relates to a manchette binding protein and effects thereof during spermiogenesis, and discloses application of rab interaction molecule binding protein 3 (RIM-BP3) or an accelerator or an inhibitor thereof in preparing a composition for regulating the spermioteleosis of male mammals. The invention also discloses a compound for regulating the spermioteleosis of the male mammals, which comprises the RIM-BP3 and Hook1 protein. The invention also discloses a method for selecting the potential substance for regulating the spermioteleosis of the male mammals.

The present invention provides a new apparatus/system for determining fertility of mammalian sperm based on a specific sperm-binding test that mimics sperm binding to an egg. The inventive apparatus/system comprises surface-modified beads with a compound or conjugates containing a carbohydrate sequence associated with the mammalian zona-pellucida (ZP) glycoproteins.

The invention provides a method for rapidly typing mammal sperms by using biologically modified beta-cyclodextrin nano magnetic particles, by which a large amount of sperms can be separated even in a short time, and the motility of the separated sperms can be prevented from being reduced. On one hand, the nano magnetic particle can be used as a carrier of biomacromolecules, and because the nano magnetic particle is coated with a TLR7/TLR8 ligand, the nano magnetic particle not only has the characteristic of specifically recognizing X/Y sperms, but also has the characteristic of magnetism, so that the nano magnetic particle has more advantages in the aspects of separating, purifying and concentrating target sperms from a complex sample and the like; the method has the advantages of rapidness (5-20 minutes), high specificity, simplicity and convenience in operation, wide application range (almost applicable to treatment of all samples) and the like. The defects of low separation efficiency, relatively strong interaction among particles and relatively poor dispersity of conventional micron-sized immunomagnetic particles can be overcome.