4 results about "Mammalian sperm" patented technology

The invention relates to the field of biological detection and toxicological evaluation, and discloses an in-vitro detection method for screening or evaluating sperm motility toxicity of a compound. The method is realized by detecting a group of specific biomarkers in mammalian sperm treated by a tested compound, and the marker combination comprises a phosphoserine / threonine PKA substrate level, an AKAP3 protein level and an AKAP4 protein level. Researches show that environmental toxin fenvalerate can specifically reduce the levels of the markers by destroying cAMP / PKA signal channel, so that sperm motility is reduced. Through quantitative analysis of changes of the markers, whether a tested compound has a similar sperm direct toxicity effect or not can be specifically and efficiently evaluated, and a new tool is provided for chemical safety evaluation and male infertility pathogenesis diagnosis.

The invention belongs to the field of medicines for treating male infertility diseases, and discloses application of 12-ketolithocholic acid (12-keto-LCA) in the aspect of improving sperm quality decline, namely application of 12-keto-LCA in preparation of medicines for improving sperm quality decline. The invention provides a new application of 12-keto-LCA, reveals for the first time that 12-keto-LCA can support the spermatogenesis process, especially can improve the reproductive function of obese males, realizes the effects of improving testosterone secretion, repairing the spermatogenic function, improving the sperm quality and embryonic developmental ability and the like, and improves the condition that the sperm quality of mammals is reduced. The 12-keto-LCA can effectively relieve dyszoospermia of males, especially relieve damage of obesity to the reproductive function of males, and a new way is provided for developing drugs for improving the reproductive function of obese males.

The invention relates to an improved method for analysing sperm DNA structure, by means of which it is possible to reduce the time required to process samples by 80%, optimising every stage of the process. This effect is achieved through a synergetic improvement in each step of the process. The formation of microgels is accelerated with cold metal plates (2–8°C) and using 0.8%-1.5% agarose. DNA denaturation is carried out using HCl (100 mM). The deproteinisation solution contains DTT (0.22 M), NaCl (2.75 M), detergents of the alcohol ethoxylate (0.044 M) and SDS (0.0014 M) type. Fixation is carried out using 96% ethanol at a low temperature. The staining process uses a panoptic dye, improving the quality of the images. The invention not only speeds up the analysis by reducing the time required by 80%, but also enables faster and more accurate diagnoses, thanks to the high quality of the images obtained.