Method for rapidly typing mammal sperms by using biologically modified beta-cyclodextrin nano magnetic particles
A nano-magnetic particle, magnetic nano-particle technology, applied in the fields of nanotechnology, animal cells, nanotechnology, etc. for materials and surface science, can solve the problems of strong interaction, poor dispersion, low separation efficiency, etc., and achieve a wide range of applications Value, uniform and stable coating effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0030] Example 1 Preparation method of nano-magnetic particles
[0031] (1) Preparation of biologically modified β-cyclodextrin nanomagnetic particles:
[0032]Amino-modified magnetic nanoparticles: accurate weighing of FeCl 2 ·4H 2 O (1.25g), add 7.75mL of double distilled water to dissolve, add 6.25mL of ammonia water under vigorous stirring, continue to stir for 10 minutes, then add 2.5mL of 3-aminopropyltriethoxysilane (APTES), at 134 ℃ Autoclave for 3 h at temperature and collect the black magnetic solution. The reaction product was centrifuged at 1000 rpm for 1 minute, after removing large particles, the supernatant was collected, centrifuged at 6000 rpm for 5 minutes, the black precipitate was collected, washed with double distilled water for 3 times, and washed with absolute ethanol twice (to remove the unreacted APTES in the solution). ), the precipitate was washed twice with double distilled water, and finally the magnetic particles were dispersed in 5 mL of doubl...
Embodiment 2
[0037] Example 2 Sperm typing and separation using biomodified β-cyclodextrin nanomagnetic particles
[0038] Select the qualified fresh swine semen as the experimental sample and use the density meter to detect the actual concentration of the original semen, add 100 million fresh semen to 5 ml of 0.1% PBS, mix gently, and centrifuge at 1500 rpm for 7 min at room temperature;
[0039] Remove the supernatant, add 300 μL of 0.01mol / L PBS, 0.5% BSA, pH7.2 adsorption solution, shake and mix well, add an appropriate amount of the TLR7 / TLR8 ligand-coated nanomagnetic particle suspension prepared in Example 1 (3- 5 μl), after mixing, incubate at room temperature for 30 min in the dark;
[0040] After the incubation, place the above centrifuge tube on the magnetic separation rack for 5 minutes to absorb and discard the liquid (reserve part for subsequent experiments); add 300 μL of 0.01mol / L PBS, 0.5% BSA, 2mM EDTA, pH7.2 for washing Liquid washing, magnetic separation rack adsorptio...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com