298 results about "Castration" patented technology

LHRH analogues and LHRH antagonists for use in the treatment or prophylaxis of hormone-dependent cancers, in particular prostate cancer, prostate carcinoma and/or advanced prostate carcinoma, by administering an initial dose of an LHRH analogue over a first period sufficient to effect hormonal castration, then administering a maintenance dose of an LHRH antagonist over a second period, the dose being insufficient to achieve and/or maintain hormonal castration.

The invention provides an application of application of plant alpha-diastase in pollen abortion, and belongs to the technical field of genetic engineering. Through introducing polynucleotide of the plant alpha-diastase, recombinant vector and recombinant vector are structured; a pollen specific promoter drives the plant alpha-diastase to degrade starch in advance during the pollen developmental phase; the pollen sprouting energy cannot be provided, and the pollen sprouting is contained, thus abortion is generated; the transgenetic seedling of transgene pollen abortion is obtained. The plant alpha-diastase can be used as the pollen abortion gene and can be originated from genes of rice endogenesis, millet, broomcorn, barley, wheat and other gramineous plants; the plant alpha-diastase is very good for transgenic plant of pollen abortion. The plant alpha-diastase gene has accurate expression level and can control the spreading of transgenosis; the plant alpha-diastase gene also can keep and breed a male sterility line while leave out the artificial castration step during the hybrid seed production process; the application prospect is wide.

The invention relates to a cultivation method of crops, in particular to a high-yield cultivation method of high-yield high-quality and high-benefit summer maize. The high-yield cultivation method comprises the following steps of: seed selection: selecting Huafeng NO.8; treatment before seeding: 1, drying seeds in the sun; and 2, dressing seeds or soaking seeds; seeding at proper time, wherein the seeding time is between September 20 and September 25, and the number of plants per mu is 4000-4200; weed prevention and weeding of maize land: weeding after seeding and before emergence of seedlings; disease and insect pest prevention and control: evenly spraying Fuge in the period from the emergence of seedlings to 3-5-leaf stage; increase of fertilizing amount, wherein fertilizing amount per mu is 75 kg of multielement fertilizer; alternate-row castration and artificially supplementary pollination: artificially castrating one row every two rows when tassel just grows parietal lobe and does not loose powder and carrying out artificially supplementary pollination at the same time. The high-yield cultivation method of summer maize is simple and easy to implement and has hihg maneuverability, and the yield per mu of maize cultivated by the method provided by the invention is 550-600kg.

The invention discloses a gene ABCG15 for controlling rice fertility, an encoded protein and the application thereof. The invention also discloses a single recessive nuclear sterile gene, which is generated by mutation caused by deletion of the gene 12bp for controlling the rice fertility. The invention also discloses a method for obtaining a rice sterile line by controlling the expression of the rice fertility gene according to an RNAi technology and the method comprises the following steps of: amplifying a DNA fragment for generating an anti-sense RNA, constructing an expression vector, and transforming the DNA fragment into the normal fertile rice, so as to obtain a new rice nuclear sterile material. The newly found gene for controlling the rice fertility provides a new way for culturing the rice nuclear sterile line. The method for generating the rice nuclear sterile line is convenient and quick. The rice nuclear sterile material can be used for replacing the manual castration during the rice hybridization, labor is saved; the rice nuclear sterile material also can be used for recurrent selective breeding and plays an important role in widening the rice germ plasm basis.

The invention relates to application of male sterility gene OsLAP5 and a method for recovering rice male sterility, belonging to the technical field of rice breeding. A base sequence of the male sterility gene OsLAP5 is as shown in SEQ ID NO.2. The application is as follows: the OsLAP5 gene is knocked out or inhibited so that activity of rice sporopollenin synthetase loses or expression level of the rice sporopollenin synthetase drops; and an obtained new rice male sterile line can be used for producing hybrid seeds. Furthermore, the invention relates to a method for creating a rice male sterile strain, application of the rice sterile strain in production of rice seeds and a method for recovering a male sterile character. The rice mutant disclosed by the invention has no abnormity in a vegetative growth stage, but a homozygote plant is completely sterile. The method, if applied to hybrid seeding, can avoid an operation of castration of female parent, greatly improve production efficiency and reduce labor cost, and the method has good application on agricultural production.

The invention relates to a hybridization breeding pollination method for peony, which comprises the following steps of: a, selecting a female parent and a male parent according to a breeding target; b, carrying out artificial castration on the female parent; c, collecting pollen; d, carrying out artificial pollination, wherein a pollination method adopts a method for bending stigmas to dip the pollen; e, bagging and marking; and f, harvesting and sowing seeds, wherein the seeds blossom out successively after being sown for 5 to 6 years. In the method, artificial pollination is carried out in time by a simple and easy method so as to obtain more hybrid progenies and quicken the directed hybridization breeding process of the peony. The adopted method for bending the stigmas to dip the pollen not only solves the problem of easy mixing of the pollen and avoids mechanical damage of pollination tools to the stigmas in the pollinating process, but also can save masses of pollen, guarantee the purity of the pollen of the male parent and improve the maturing rate of hybridization, particularly distant hybridization. The method has the advantages of simple operation, easy popularization and capability of greatly improving the working efficiency of the breeding industry of the peony and similar plants.

The invention provides a sexual hybridization method for abelmoschus esculentus. The sexual hybridization method comprises the following steps of: 1. selecting a flower bud on a female parent, which can bloom in the second day in the afternoon of a sunny day; 2. aslant cutting in a place far away from a base part of the flower bud by 0.4-0.6cm for 0.4-0.6cm by using a pencil knife, cutting around the flower bud by 3-4 times, cutting out a corolla to expose a style, scraping a stamen on a stamen tube from bottom to top by using a knife back, wherein anthocaulus is marked, a parchment tube is sleeved on the style, the diameter of the parchment tube is 0.7-0.9cm, the length of the parchment tube is 3.5-4.0cm, and one end of the parchment tube is closed; and 3. extending a small box for collecting pollen into a male parent flower at 7:00-10:00 in the morning of the second day, slightly tapping the base part of the flower by using index fingers to ensure that the pollen falls in the small box without picking the flower, wherein the parchment tube on the style of the female parent is taken off, the pollen is coated on the stigma by using a writing brush, and the parchment tube is sleeved after the pollination is finished. According to the sexual hybridization method, the complete stigma and the stamen tube are remained when the female parent is subjected to castration, and thus the castration success rate and the working efficiency can be improved. The parchment tube is sleeved on the style, and thus the hybridized seed purity and the legume forming rate can be increased. The flower is remained when the pollen is taken, and thus a tender legume can be harvested.

The invention discloses application of a male sterility gene OsDPW3 and a rice fertility restoration method. The gene OsDPW3 is knocked out, changed or inhibited by using the conventional genetic engineering method or based on a CRISPR/Cas9 system, and the OsDPW3 gene expression level is decreased in wild type rice with japonica rice background, so that a rice male sterile line is further obtained. The invention also relates to the rice fertility restoration method. According to the method, the OsDPW3 gene is amplified by means of primers, and mutant plants are transformed by using a genetic transformation means, so that the mutant can be restored to a wild-type phenotype. The rice male sterility line prepared by the method has no abnormal phenotype in the rice vegetative growth period, but abnormality occurs in an anther development process of the reproductive growth period. If the rice male sterility line is applied to cross breeding, the work of castration of a female parent can beeliminated, so that the production efficiency is greatly improved, and the labor cost is lowered. The male sterility gene OsDPW3 has important application to agricultural production.

A method for non-surgical neutering or castration of a non-human mammal for AAV-mediated delivery of an anti-GnRH polypeptide to a non-human animal is described. More particularly, the animal is administered an adeno-associated virus (AAV) vector having an AAV capsid having packaged therein nucleic acid sequences comprising an AAV 5′ inverted terminal repeat (ITR), a sequence encoding a polypeptide which specifically binds gonadotropin releasing hormone (GnRH) under control of regulatory sequences which direct expression of the polypeptide, and an AAV 3′ ITR. A composition comprising the AAV-anti-GnRH may also be used for inhibiting tumor growth in a mammal with a cancer responsive to gonadal steroid hormones.

An easily operated castrating tool that allows placement of an improved continuous elastic band for ligation and removal of an appendage. The present invention includes a tool that allows such bands to be placed in a manner that is significantly more easy to use, and less fatiguing to the parties that utilize the device. The instant invention is also an improved elastic band which allows for better compressive ligation about a body part as well as a simple, but effective, spreading tool that allows for quicker and easier placement of the improved elastic band about a body part to be ligated.

The invention discloses a positive and reversed distant hybridization method of tideland smooth cordgrass and rice. The positive and reversed distant hybridization method comprises the following steps of sorting spikes, carrying out castration, pollinating and the like in positive and reversed hybridization processes. The positive and reversed distant hybridization method comprises (1) positive hybridization: rice (female)*smooth cordgrass (male): a method of carrying out hot air castration and flower promoting and pollen suction on rice (female), and pollinating smooth cordgrass (male) properly is adopted; rice spikes are treated by hot gas and anthers are adsorbed and removed by a pollen suction device, so that a process of killing male by warm liquid and carrying out spikelet shearing castration of a rice female parent is omitted; proper pollination is adopted so that a spikelet shearing flower promoting process of a smooth cordgrass male parent is omitted, and seeds obtained by hybridization are unhusked rice, but not rice grains, and the seeds have good grain-filling rate; and (2) reversed hybridization: smooth cordgrass (female)*rice (male): a continuous castration and pollination process of the smooth cordgrass (female) is adopted. The castration is carried out while the pollination is carried out so that a process of killing male of the smooth cordgrass (female) by the warm liquid and carrying out rice flower promotion of the smooth cordgrass (female) is omitted, and damage to spikelets is reduced. With the adoption of the two positive and reversed hybridization methods, the hybridization maturing rate, the emergence rate and the working efficiency are greatly improved. The positive and reversed distant hybridization method can be used for cultivating smooth cordgrass/rice anti-salt grain and feed dual-purpose type crops which are suitable for growing in saline and alkaline lands.

The invention discloses a cowpea isolating hybridization pollination method. The technical solution is that: choosing male parent and female parent of cowpea with excellent performance; selecting hale anthotaxy bud 1 to 3 days before the female parent flower open and suiting their necking with partition to separate the gynoecia from the androecium; collecting farina of the unopened male parent in a farina box for standby and then place the farina of the male parent into the partition for pollination on the next day of isolating the necking to complete the hybridization and marked with labels. The invention saves the castration procedure, which features simple operation, time and labor-saving and free of damaging the flower's natural structure and affecting the normal growth conditions for germ cell with high breeding success rates; besides, the invention, by applying the partition to suit the necking to isolating the gynoecia and the androecium, avoids the self cross and hybridization and effectively reduces the fake hybridization rate.

The invention discloses a cultivation method for new okra varieties. The cultivation method includes seeding and planting management and sexual hybridization of okra parents, pollen activity of the okra parents is tested, the okra parents are assisted in sexual hybridization by means of manual castration and pollination when the pollen activity of the okra parents reaches more than 30%, seeds are collected, hybrid progenies are screened and purified, expressed characters of the parents and the hybrid progenies are compared in the same planting environment, the hybrid progenies with excellent characters are screened out and inbred, and the excellent characters are purified and stabilized. The okra varieties 'Wu Fu' and 'pentagon' are used as female parents, red okra is used as a male parent, sexual hybridization is performed by means of manual pollination, and the obtained new okra varieties have the advantages of nutrient-rich pods, long collecting period, high yield per mu and the like as compared with the female parents 'Wu Fu' and 'pentagon', have the advantages of excellent pod quality, short plant form, lodging resistance and the like as compared with the male parent red okra, and are novel healthcare new vegetable varieties with excellent quality.

The invention discloses a high-efficiency pea cross breeding method. The high-efficiency pea cross breeding method comprises the following steps of: (1) performing castration, namely selecting a bud to bloom from a female parent plant, holding the torus lightly with the hand, clamping the position 3.5 to 4.5 cm away from the top of vexil and the connecting position of the vexil and the torus by using forceps, and clamping and pulling out petals, wherein the included angle of the forceps and the bud back line is 40 degrees; (2) performing pollination, namely selecting a male patent flower withpollen to be scattered or scattered just now, taking out pollen grains to place into a culture dish, clamping the pollen grains scattered just now by using the forceps, and coating the pollen on the tomentum of an exposed stigma of the female parent flower; (3) fastening a label; and (4) pruning. By the method, all the petals are pulled out at one step by the forceps, so the castration speed is high and the castration is performed completely; the stigma is not damaged; the working efficiency is three times higher than that of the traditional method and two times higher than that of a Suzonganmethod; success rate of hybrid fructification is increased by 33 percent compared with that of the traditional method and is increased by 13 percent compared with that of the Sizongan method; and theoccurrence probability of descendant pseudo hybrid is 0.

The invention discloses a corn haploid inducing method. The method comprises the steps of pollinating a female parent by using a corn haploid inducing system 'qiyou 201' as a male parent and harvesting current-generation hybridized grains to obtain corn haploids, wherein the planting modes of the female parent and the male parent are as follows: the female parents and the male parents are planted alternatively in an isolating zone in a row manner, and are sown in different stages to enable the female parents and the male parents to realize flower synchronization which is characterized in that when 3-5cm of filaments of the female parent grow, the male parent starts to perform pollen application, the female parent is subjected to castration before pollen application of the male parent, and subtending leaves of the female parent are cut off when the male parent performs pollen application. Experiments show that the corn haploid inducing method has the advantages that the corn haploid inducing rate is up to be more than 19% compared with the corn haploid inducing rate of the traditional method, the high-efficiency haploid inducing system 'qiyou 201' has strong growth vigor, is mid-maturation, and has high pollinating amount, long pollen dispersing duration time, wide adaptability and strong resistance, solves the technical bottleneck of low inductivity of the traditional haploid induction system and provides technical support for large-scale haploid induction in commercialized corn breeding.

A pharmaceutical composition using natural gonadotropin-releasing hormone (GnRH), and/or some of its mimetic peptides, indistinctly bound by its amino or carboxyl extremes to a carrier molecule; in one case by its carboxyl extreme and in the other case by the amino terminal extreme, thus eliciting a faster and more potent immunological response against the endogenous GnRH hormone. This finally leads to the ablation of the GnRH and consequently of the rest of the involved hormones in the stream GnRH/LH-FSH/Testosterone-(estrogens). An advantage of this formulation consists on facilitating the exposition to the immune system of a greater number of epitopes of the GnRH or its mimetics, minimizing thus the steric hindrance produced by the carriers. This invention has a direct application in the castration of pets and animals of economic interest, in the control of human fertility as well as in the treatment of hormone-sensitive tumors, such as that of the prostate, the breast, ovary, the endometry, testicles, hypophysis, salivary glands and other kinds of human tumors.

The invention relates to a maize plant protection and castration machine, in particular to a multifunctional maize pesticide spraying and castration machine. The multifunctional maize pesticide spraying and castration machine comprises a cab, an engine, a hydraulic pump, a chassis device, a spraying device and a castration device. The multifunctional maize pesticide spraying and castration machine is characterized in that the chassis device comprises a vehicle frame, a front support, front wheels, a rear support and rear wheels, the spraying device comprises a pesticide box, a diaphragm pump, a spraying rod lifting frame and a spraying rod device, the castration device comprises a castration lifting frame and a castration idler wheel set, the spraying rod lifting frame is connected to the rear portion of the vehicle frame, the spraying rod device is connected to the rear portion of the spraying rod lifting frame, the castration lifting frame is connected to the front portion of the vehicle frame, and the castration idler wheel set is connected to the front portion of the castration lifting frame. The multifunctional maize pesticide spraying and castration machine is low in overall gravity center, reasonable in structure, high in use rate and the like. The multifunctional maize pesticide spraying and castration machine can conduct castration and pesticide spraying.

The invention provides a rice hybridization and castration method, belongs to the technical field of crop breeding, and particularly relates to a novel rice hybridization and emasculation-water spraying-castration method. The rice hybridization and castration method comprises the following steps: 1, selection of a reasonable sowing date; 2, selection of rice plants; 3, pruning of rice ears; 4, emasculation, water spraying and castration; 5, timely and efficient pollination. Through the adoption of the rice hybridization and castration method, the problem that the conventional rice hybridization work fails due to high labor intensity, incomplete castration and damage to pistil is solved; the rice hybridization and castration method has the advantages that the labor consumption and the cost are reduced, the operation is simple and convenient, the hybridization success rate is high, and the economical and environment-friendly effect is achieved, is deeply welcomed by scientific research breeding workers, and has a broad application prospect.

The invention discloses a binary hybridization method of quinoa and relates to the technical field of crop breeding. The binary hybridization method solves the problem that the quinoa hybridization has strong labor intensity, incomplete castration and pistil damage causing hybridization failure. The method comprises: selecting female and male plants needing hybridization, and carrying out binary hybridization on quinoa through an artificial pollination method, wherein the flower of the female plant used by binary hybridization has a pistil mature in advance of a stamen and is fully opened whenthe pistil is mature and the stamen is immaturate and the pistil is fully exposed. The specific quinoa female plant can break through the trouble of the complicated artificial emasculation and laterbreeding, and realize hybridization through matching of a breeding target and a male parent. The method breaks through the quinoa breeding technology and is fast, accurate and efficient.

The invention relates to a natural-hybrid seed production method for triploid salvia which takes the purple genes as marker traits; the method takes tetraploid white salvia as the female parent and diploid purple salvia as the male parent; in the field, the male parent and the female parent are planted according to a proportion of 1 to 1; pollination is done by wild hornet and domesticated bees; 2/3 of the seeds produced on the tetraploid white salvia female parent plant are triploid salvia, while the remaining 1/3 are tetraploid white salvia; the hybrids with purple flowers are triploid salvia, while the hybrids with white flowers are tetraploid salvia; the triploid salvia is slightly purple at stems, leaves and petioles at seedling stage; the invention does not need manual castration, thereby reducing the labor intensity in seed production, saving the labor and decreasing the production cost; besides, the parental seeds can be accurately separated from the hybrid seeds, and the produced seeds have high purity. The invention can be applied to larger-area hybrid seed production and is easy to promote.

The invention provides a cross breeding method of macadimia nuts and belongs to the field of cross breeding technologies of fruit trees. The cross breeding method comprises three steps of carrying out castration, collecting pollen and artificially pollinating. The step of carrying out castration comprises the following processes: selecting inflorescences with most paled flower buds, cracked perianth tubes and flower columns protruding the 1dth during full-bloom stages of the macadimia nuts, removing little flowers with uncracked perianth tubes, pruning the little flowers with the flower columns protruding the 1dth till each flower bud retains 30-100 small flowers, cutting off the perianth tubes in protruded and bent parts of the flower columns of the little flowers, removing perianth pieces when partially turning parts of the cut perianth tubes outwards or after cutting the perianth tubes for 10-30 minutes, covering the flowers with bags and sealing to obtain castration inflorescences. By virtue of the method, the castration efficiency of the macadimia nuts is effectively improved, the artificial castration is relatively simple and quick, the generation of false hybrid is avoided, and the purity of hybrids is improved.

The invention discloses a green healthy live pig breeding method. In the method, the pig house size, the feeding management, the feed management and the environmental management are scientifically controlled, wherein the feeding management comprises the following steps: after a piglet is born, the teeth are cut, the tail is broken, and an iron supplementation injection is given to the piglet within seven days, and in 11-13 days, castration is carried out, when the piglet is 20-22 days old, the pig is inoculated with swine fever vaccine, the pig is weaned and fed with suckling pig feed; after the pig is fed with the suckling pig for 9-11 days, the pig is fed with nursery feed, and when the pig is 14-16.5 kilograms, the pig is fed with piglet feed, and when the pig is 34-36 kilograms, the pig is fed with middle pig feed, and when the pig is 100-105 kilograms, the pig is fed with fattening feed. The feed management comprises the following steps: the weight ratio of one of the suckling pig feed, the nursery feed, the piglet feed, the middle pig feed or the fattening feed to forage is 1:2-2.5; and the pig is fed with the feed every 7-8 hours. The pig house environment can be well adapted to the growth of live pigs, occurrence of pig disease can be reduced, the daily gain of the live pigs can be increased, the feed-weight ratio can be reduced, the anti-disease ability of the live pigs can be improved, the meat quality of the pork is good, and the pork is green and safe.

The present invention provides at least one LHRH antagonist for the treatment or prophylaxis of at least one lower urinary tract symptom in mammals which is to be administered in an intermediate dose, which does not cause chemical (hormonal) castration.